Proliferation and TH1/TH2 Cytokine Production in Human Peripheral Blood Mononuclear Cells after Treatment with Cypermethrin and Mancozeb In Vitro by Rajesh Mandarapu, Rajanna Ajumeera, Vijayalakshmi Venkatesan, Balakrishna Murthy Prakhya

“…Human peripheral blood mononuclear cells (PBMC) were isolated and exposed for 6 hr to noncytotoxic doses (0.45–30 µM) of cypermethrin or mancozeb in the presence of activating rat S9 fraction. Cultures were then further incubated for 48 or 72 hr in fresh medium containing phytohemagglutinin (10 µg/mL) to assess, respectively, effects on cell proliferation (BrdU-ELISA method) and cytokine formation (flow cytometric bead immunoassays). …”
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Identification of microorganisms at different times in a bioleaching process for the recovery of gold and silver from minerals in oxide form by Cuauhtémoc Contreras Mora, Juan Antonio Rojas Contreras, Mayra Cristina Rosales Villarreal, José Luis Urban Martínez, Efren Delgado, Hiram Medrano Roldan, Felipe Samuel Hernández Rodarte, Damián Reyes Jáquez

“…Native bacteria and varying ratios of mineral pulp to culture medium (20/80, 37.5/62.5, and 50/50 %) from a mining operation in Zacatecas, Mexico, were evaluated. …”
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Influences of Temperature on Development and Survival, Reproduction and Growth of a Calanoid Copepod (Pseudodiaptomus dubia) by Changling Li, Xiaoxia Luo, Xianghu Huang, Binhe Gu

“…Pseudodiaptomus dubia is a calanoid copepod that is distributed widely in the estuarine-coastal waters of Asia and is a dominant copepod in the shrimp grow-out ponds in southern China. A laboratory culture experiment was conducted to evaluate the influences of water temperature on larval development, survival, and reproduction. …”
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Intrauterine Growth Restriction: Cytokine Profiles of Trophoblast Antigen-Stimulated Maternal Lymphocytes by Raj Raghupathy, Majedah Al-Azemi, Fawaz Azizieh

“…Peripheral blood mononuclear cells were stimulated with trophoblast antigens and levels of the proinflammatory cytokines IL-6, IL-8, IL-12, IL-23, IFNγ, and TNFα and the anti-inflammatory cytokines IL-4, IL-10, and IL-13 were measured in culture supernatants by ELISA. IL-8 was produced at higher levels by blood cells of the IUGR group than normal pregnant women, while IL-13 was produced at lower levels. …”
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Nigeria's Adoption of Robotic Lawyers: Legal and Socio-Economic Challenges by Paul Atagamen Aidonojie, Obi Eregbuonye, Adesoji Kolawole Adebayo, Shedrack Ekpa, Ketut Sukewati Lanang Putra Perbawa

“…Furthermore, some socio-economic issues such as internet fraudster, unemployment, insecurity, and poor maintenance culture may pose a challenge to the adoption of a robotic lawyer in Nigeria. …”
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Liver macrophage-derived exosomal miRNA-342-3p promotes liver fibrosis by inhibiting HPCAL1 in stellate cells by Wenshuai Li, Lirong Chen, Qi Zhou, Tiansheng Huang, Wanwei Zheng, Feifei Luo, Zhong Guang Luo, Jun Zhang, Jie Liu

“…Method Exosomes were extracted from primary mouse macrophages and cultured with HSCs, and the differential expression of microRNAs was evaluated using high-throughput sequencing technology. …”
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Report on Intersex and Abnormal Mature Aquacultured Walleye Pollock, <i>Gadus chalcogrammus</i> by Hae-Kyun Yoo, Soo-Ji Woo, Ki-Wook Lee, Min-Soo Joo, Kyeong-Duck Kim, Jung-Jun Park, So-Sun Kim

“…Walleye pollock (<i>Gadus chalcogrammus</i>) is a commercially important species widely distributed in cold-water regions. We have been culturing this species artificially since 2015. The average embryo diameter was 1.43 ± 0.056 mm, and hatching occurred at 5 °C approximately 339 h post-fertilization. …”
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Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 by Zhiying Ai, Jingjing Shao, Xinglong Shi, Mengying Yu, Yongyan Wu, Juan Du, Yong Zhang, Zekun Guo

“…Embryonic stem cells (ESCs) have the ability to grow indefinitely and retain their pluripotency in culture, and this self-renewal capacity is governed by several crucial molecular pathways controlled by specific regulatory genes and epigenetic modifications. …”
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Human Suprapatellar Fat Pad-Derived Mesenchymal Stem Cells Induce Chondrogenesis and Cartilage Repair in a Model of Severe Osteoarthritis by Ignacio Muñoz-Criado, Jose Meseguer-Ripolles, Maravillas Mellado-López, Ana Alastrue-Agudo, Richard J Griffeth, Jerónimo Forteza-Vila, Ramón Cugat, Montserrat García, Victoria Moreno-Manzano

“…In addition, the suprapatellar SVF exhibited increased numbers of colony formation units and a higher population doubling in culture compared to the infrapatellar fraction. Both the suprapatellar- and infrapatellar-derived ASC were differentiated in vitro into mature adipocytes, osteocytes, and chondrocytes. …”
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Knowledge about oral health in older adults from a medical office in Ciego de Ávila by Wendy Guerra López, Lina Isabel Torres Gárriz, Evelyn Sardiñas Montero, Yenise García Aldave, Daniela Rivas Torres, Marcos Daniel Estrada López

“…<br /><strong>Conclusions</strong>: it was determined that the level of knowledge of these older adults was regular, which shows that strategies must be intensified to increase the health culture in this group.</p>…”
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Autoantibodies Targeting AT1 Receptor from Patients with Acute Coronary Syndrome Upregulate Proinflammatory Cytokines Expression in Endothelial Cells Involving NF-κB Pathway by Weijuan Li, Zhi Li, Yaoqi Chen, Songhai Li, Yuanyuan Lv, Wenping Zhou, Mengyang Liao, Feng Zhu, Zihua Zhou, Xiang Cheng, Qiutang Zeng, Yuhua Liao, Yumiao Wei

“…Antibodies were determined by chronotropic responses of cultured neonatal rat cardiomyocytes coupled with receptor-specific antagonists (valsartan and AT1-EC2) as described previously. …”
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Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation? by Tulay Irez, Sinem Ercan Dogan, Enver Ciraci, Saadet Busra Aksoyer, Muhammet Sait Toprak, Nurten Dayioglu, Onur Guralp

“…STUDY DESIGN: In this experimental study, donated immature germinal vesicle oocytes were cultured for, in vitro maturation, embryo development in matured germinal vesicle oocytes were compared with apoptotic properties of cumulus cells. …”
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Infections Caused by Actinomyces neuii: A Case Series and Review of an Unusual Bacterium by Nathan Zelyas, Susan Gee, Barb Nilsson, Tracy Bennett, Robert Rennie

“…Cases of A. neuii were identified in 2013-2014 in Alberta. Samples were cultured aerobically and anaerobically. A predominant catalase positive Gram-positive coryneform bacillus with no branching was isolated in each case. …”
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Tilapia Lake Virus (TiLV): a Globally Emerging Threat to Tilapia Aquaculture by Lowia Al-Hussinee, Kuttichantran Subramaniam, Win Surachetpong, Vsevolod Popov, Kathleen Hartman, Katharine Starzel, Roy Yanong, Craig Watson, Hugh Ferguson, Salvatore Frasca Jr, Thomas Waltzek

“… Tilapia lake virus is a globally emerging virus responsible for episodes of mass mortality in cultured and/or feral tilapia (Oreochromis spp. and hybrids) in Asia, Africa, Central America, and South America. …”
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Impact of diabetes mellitus on dental implants: A cross sectional controlled clinical study. by Rezhna Muhammed Hamad-Ameen, Othman Abu-Bakr Omer

“…Results: Diabetic patients show a Statistically significant difference to non diabetic subjects in the level of interleukin 6 with (P value 0.004), Culture and sensitivity with (P value 0.003), Peri -implant bone loss in mesial and distal sides of the implant with P values 0.006 and 0.002 respectively, and also, in gingival recession with (P value 0.017). while the difference was not significant in gingival sulcus depth P value0.220, bleeding on probing P value0.550, implant suppuration P value 0.999, and mobility P value 0.999. …”
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Warming-driven migration of enterotypes mediates host health and disease statuses in ectotherm Litopenaeus vannamei by Shenzheng Zeng, Zhijian Huang, Satapornvanit Kriengkrai, Renjun Zhou, Derun Yuan, Nguyễn Văn Tuấn, Zhiming Zhu, Luwei Zheng, Qilu Hou, Xuanting Li, Qi Chen, Lingyu Zhang, Dongwei Hou, Zhixuan Deng, Shicheng Bao, Wenjun Wang, Sukontorn Khoruamkid, Soo Loon Goh, Shaoping Weng, Jianguo He

“…These findings enlarged the cognition of shrimp health culture management from a microecological perspective, and alerted the inevitable challenge of global warming to ectothermic animals.…”
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Impairments of Antigen-Presenting Cells in Pulmonary Tuberculosis by Ludmila V. Sakhno, Ekaterina Ya. Shevela, Marina A. Tikhonova, Sergey D. Nikonov, Alexandr A. Ostanin, Elena R. Chernykh

“…The macrophages which were in vitro generated from peripheral blood monocytes under GM-CSF were characterized by Th1/Th2-balance shifting (downproduction of IFN-γ coupled with upproduction of IL-10) and by reducing of allostimulatory activity in mixed lymphocyte culture. The dendritic cells (generated in vitro from peripheral blood monocytes upon GM-CSF + IFN-α) were characterized by impaired maturation/activation, a lower level of IFN-γ production in conjunction with an enhanced capacity to produce IL-10 and IL-6, and a profound reduction of allostimulatory activity. …”
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The Effectiveness of Fluorescent Light Energy as Adjunct Therapy in Canine Deep Pyoderma: A Randomized Clinical Trial by Andrea Marchegiani, Alessandro Fruganti, Andrea Spaterna, Matteo Cerquetella, Adolfo M. Tambella, Susan Paterson

“…Dogs with clinical lesions consistent with deep pyoderma, positive bacterial culture, and showing neutrophil engulfing bacteria at cytology were included in the study. …”
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Caregiver’s Opinions on the Design of the Screens of a Future Gamified Mobile Application for Self-Management of Type 1 Diabetes in Children in Saudi Arabia by Demah M. Alsalman, Zahra Bu Ali, Zainab Alnosaier, Norah Alotaibi, Turki M. Alanzi

“…The designed screens are adapted to the Arab culture.…”
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Bimodal Porous Scaffolds by Sequential Electrospinning of Poly(glycolic acid) with Sucrose Particles by B. Wulkersdorfer, K. K. Kao, V. G. Agopian, A. Ahn, J. C. Dunn, B. M. Wu, M. Stelzner

“…After 24 hours of cell culture, conventionally spun scaffolds demonstrated no cellular penetration. …”
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