Effects of carbon sources and temperature on the formation and structural characteristics of food-related Staphylococcus epidermidis biofilms by Mingming Zou, Donghong Liu

“…Therefore, this study was conducted to investigate biofilms formation by Staphylococcus epidermidis under various conditions. Biofilms were cultured in nutrient broth containing different carbon source concentrations (0–10 mg/mL) on polystyrene surfaces for 32 h of incubation at 37 °C or 55 °C, with quantification and enumeration at 8, 16, 24 and 32 h. …”
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Antibiotic-Resistant Bacteria Are Major Threats of Otitis Media in Wollo Area, Northeastern Ethiopia: A Ten-Year Retrospective Analysis by Ayele Argaw-Denboba, Asrat Agalu Abejew, Alemayehu Gashaw Mekonnen

“…A retrospective analysis was performed using culture and antibiotic susceptibility test results of 1225 patients who visited Dessie Regional Health Research Laboratory from 2001 to 2011. …”
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Faecalibacterium prausnitzii: A Next-Generation Probiotic in Gut Disease Improvement by Xiaoya He, Shuyang Zhao, Yan Li

“…It is reported that intervention with higher richness of F. prausnitzii through dietary modulation, fecal microbiota transplantation, or culture strategy can protect the mice or the subjects from inflammatory diseases. …”
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B cells enhance IL-1 beta driven invasiveness in triple negative breast cancer by Nicole J. Toney, Lynn M. Opdenaker, Lisa Frerichs, Shirin R. Modarai, Aihui Ma, Holly Archinal, Grace O. Ajayi, Jennifer Sims-Mourtada

“…This study shows that co-culturing TNBC cells with B cells increases Interleukin-1β (IL-1β) expression and secretion. …”
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Reconstruction of ancient craftsmanship techniques during the Xiongnu-Xianbei period, based on the analysis of bone tools found at the Chultukov Log-9 site in Altai by Krzysztof Michalczewski, Łukasz Oleszczak, Konrad Lewek

“… The paper presents the results of research on bone artifacts from the Maima culture settlement of Chultukov Log-9. The site is dated to the Xiongnu-Xianbei period, specifically from the 3rd to the first half of the 6th century AD; its estimated area is around 5,000 square meters. …”
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Megoura crassicauda promote the ability of Vicia faba L. to remediate cadmium pollution of water and soil by Sijing Wan, Shasha Wang, Yan Li, Yexin Xie, Qimei Li, Yinjie Fang, Zhenjuan Yin, Shigui Wang, Yifan Zhai, Bin Tang

“…This study investigated the cadmium (Cd) remediation ability of fava beans by M. crassicauda feeding on or not using both hydroponic and soil cultures containing varying concentrations of Cd. Under hydroponic conditions, the Cd content in fava beans increased significantly following aphid invasion. while the Cd content decreased after aphid infestation under soil cultivation conditions. …”
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Diverse vaginal microbiota in healthy Japanese women: a combined relative and quantitative analyses by Masahiro Ito, Misaki Kataoka, Yoichi Sato, Hideki Nachi, Koji Nomoto, Koji Nomoto, Nobuhiko Okada

“…Additionally, the vaginal fluid was cultured on four types of selective agar plates. The predominant species in the growing colonies were identified using colony polymerase chain reaction, and the colonies were counted.ResultsThe vaginal microbiota was classified into four categories based on the characterization of the dominant bacterial population: Lactobacillus crispatus, Lactobacillus iners, Lactobacillus gasseri, and a diversity group. …”
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15-Deoxy-Δ12,14-prostaglandin J2 Induces Apoptosis and Upregulates SOCS3 in Human Thyroid Cancer Cells by Carlos Antônio Trindade-da-Silva, Carolina Fernandes Reis, Lara Vecchi, Marcelo Henrique Napimoga, Marcelo Sperandio, Bruna França Matias Colombo, Patrícia Terra Alves, Laura Sterian Ward, Carlos Ueira-Vieira, Luiz Ricardo Goulart

“…In the present study, we evaluated the effect of 15d-PGJ2 in human thyroid papillary carcinoma cells (TPC-1) using different doses of 15d-PGJ2 (0.6 to 20 μM) to determine IC50 (9.3 μM) via the MTT assay. The supernatant culture medium of the TPC-1 cells that was treated either with 15d-PGJ2 or with vehicle (control) for 24 hours was assessed for IL-6 secretion via CBA assay. …”
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Patterns of Antimicrobial Resistance in the Causative Organisms of Spontaneous Bacterial Peritonitis: A Single Centre, Six-Year Experience of 1981 Samples by Sara Sheikhbahaei, Alireza Abdollahi, Nima Hafezi-Nejad, Elham Zare

“…SBP was found in 482 (24.33%) of samples, of which 314 (65.15%) were culture positive. The most prevalent isolated pathogen was E. coli (33.8%), followed by staphylococcus aureus (8.9%) and Enterococcus (8.6%). …”
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Cloned airway basal progenitor cells to repair fibrotic lung through re-epithelialization by Yu Zhao, Yueqing Zhou, Weipan Zhang, Mingzhe Liu, Jun Duan, Xiaopeng Zhang, Qiwang Ma, Yujia Wang, Yuzhen Zhang, Zhongliang Guo, Ting Zhang, Wei Zuo

“…Aided with an automated culture system, we clone and characterize airway basal progenitor cells from 44 donors with various lung conditions. …”
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Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells by Fu-qing Zhong, Yang Li, Xian-qiang Mi

“…Plasmids encoding Kir6.2 was constructed and heterologously expressed in cultured mammalian HEK-293 cells. The patch-clamp and data acquisition systems were used to record KATP channel current before and after irradiation. …”
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Tumor-associated neutrophils and macrophages interaction contributes to intrahepatic cholangiocarcinoma progression by activating STAT3 by Jian Zhou, Jia Fan, Zhiqiang Hu, Jia Li, Zhengjun Zhou, Pengcheng Wang, Rongqi Sun, Haoyang Xin, Chubin Luo, Shaolai Zhou

“…TANs and TAMs produced higher levels of oncostatin M and interleukin-11, respectively, in co-culture than in monoculture. Both of those cytokines activated STAT3 signaling in ICC cells. …”
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Conservation of the West Sayan endemic Fritillaria sonnikovae Schaulo et A. Erst (Liliaceae) in in vitro collection by D. S. Muraseva, N. S. Zvyagina, T. I. Novikova, O. V. Dorogina

“…The optimum nutrient medium for stages of in vitro culture initiation and multiplication was BDS supplemented with BAP (5.0 µM) and NAA (2.0 µM). …”
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Borrelia burgdorferi tolerates alteration to P66 porin function in a murine infectivity model by Christa H. Fierros, Marie-Line Faucillion, Beth L. Hahn, Phillip Anderson, Mari Bonde, Julie R. Kessler, Matthew C. Surdel, Kyler S. Crawford, Yan Gao, Jieqing Zhu, Jieqing Zhu, Sven Bergström, Jenifer Coburn, Jenifer Coburn

“…Vancomycin treatment of B. burgdorferi cultures was used to screen for P66 porin function and found to generate spontaneous mutations in p66 (bb0603). …”
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Predisposing Factors, Microbial Characteristics, and Clinical Outcome of Microbial Keratitis in a Tertiary Centre in Hong Kong: A 10-Year Experience by Alex Lap-Ki Ng, Kelvin Kai-Wang To, Chile Chi-Lai Choi, Leonard Hsu Yuen, Suk-Ming Yim, Keith Shun-Kit Chan, Jimmy Shiu-Ming Lai, Ian Yat-Hin Wong

“…Results. 347 scrapes were performed in the 10-year period growing 130 microorganisms (32.3% culture positive rate). Contact lens use was the commonest risk factor. …”
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Occupational therapy in overweight and obesity care: Australian perspectives from a mixed methods study by Kieva Richards, Olivia Beattie, Danielle Hitch, Genevieve Pepin

“…Three key themes were identified: 1) Exploring clients’ needs for weight management; 2) Incorporating weight management strategies in occupational therapy intervention; and 3) Organisation of current occupational therapy practice for people with obesity.Conclusion Occupational therapists should leverage an occupational perspective to enhance participation and engagement for people with obesity, thereby ensuring the client’s best interests are met.Significance As change agents, occupational therapists can advocate for shifts in care culture, influence leadership and challenge systemic issues that limit occupational performance and participation for people with obesity.…”
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Detection of Eight Respiratory Bacterial Pathogens Based on Multiplex Real-Time PCR with Fluorescence Melting Curve Analysis by Liuyang Hu, Bing Han, Qin Tong, Hui xiao, Donglin Cao

“…A total of 157 sputum specimens were examined by multiplex real-time with fluorescence MCA, and the results were compared with the conventional culture method. Results. Multiplex real-time PCR with fluorescence MCA specifically detected and differentiated eight respiratory bacterial pathogens by different melting curve peaks for each amplification product within 2 hours and exhibited high repeatability. …”
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Interleukin-18 Increases TLR4 and Mannose Receptor Expression and Modulates Cytokine Production in Human Monocytes by Luciane Alarcão Dias-Melicio, Reginaldo Keller Fernandes, Daniela Ramos Rodrigues, Marjorie Assis Golim, Angela Maria Victoriano Campos Soares

“…Monocytes from healthy donors were stimulated or not with IL-18 for 18 h, and then the TLR2, TLR4, and MR expression and intracellular TNF-α, IL-12, and IL-10 production were assessed by flow cytometry and the levels of TNF-α, IL-12, IL-15, and IL-10 in culture supernatants were measured by ELISA. IL-18 treatment was able to increase TLR4 and MR expression by monocytes. …”
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Clinical diagnosis of Q fever by targeted next-generation sequencing for identification of Coxiella burnetii by Shaohua Zhan, Haoyuan Jin, Hanbing Ji, Xin Hou, Jing Li, Ye Zhang, Jiajia Zheng, Liyan Cui

“…The mean age of the patients was 39.6 years (range: 32–59 years). Although blood cultures were negative, elevated infection markers (CRP, PCT, and ferritin) and liver enzymes (ALT, AST, GGT, ALP, and LDH) were observed. …”
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Identification of Epinastine as CD96/PVR inhibitor for cancer immunotherapy by Xiangrui Zhang, Lihan Zhang, Beibei Li, Qingchao Wang, Peixin Chen, Ranran Shi, Xiuman Zhou, Xiaoshuang Niu, Wenjie Zhai, Yahong Wu, Wenhui Shen, Xiaowen Zhou, Wenshan Zhao

“…Our results demonstrated that Epinastine exhibited high affinity for CD96, thereby effectively disrupting the interaction between CD96 and PVR. In vitro co-culture experiments further revealed that Epinastine effectively restored the ability of Jurkat cells to secrete IL-2. …”
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