Miniaturization and characterization of patient derived hepatocellular carcinoma tumor organoid cultures for cancer drug discovery applications by David A. Close, Paul A. Johnston

“…We describe the miniaturization and growth in 96- and 384-well formats of a single non-tumor liver and two Hepatocellular carcinoma (HCC) organoids derived from cryopreserved PDTO cells and the application of high content imaging (HCI) to characterize the models and enhance drug sensitivity testing. …”
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Heart rhythm safety of the mononuclear cord blood stem cells transplantation in patients with refractory ischemic heart failure by O.Yu. Usenko, A.V. Yakushev, O.V. Lisun, M.V. Kostylev, V.F. Onishchenko

“…Results demonstrated that administration of the cell preparation «Cryopreserved human cord blood» is not associated with increased risk of life-threatening cardiac arrhythmias. …”
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Assessing the potential of germplasm collections for the management of genetic diversity: the case of the French National Cryobank by Jacques, Alicia, Duclos, Delphine, Danchin-Burge, Coralie, Mercat, Marie-José, Tixier-Boichard, Michèle, Restoux, Gwendal

“…In response, many countries, including France, have developed gene banks in order to conserve reproductive genetic material. Cryopreserved resources can potentially be used to manage the genetic diversity of populations, but this opportunity is seldom exploited. …”
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Ocular Involvement and Blindness Secondary to Linear IgA Dermatosis by Cinthya Ramos-Castellón, Gabriela Ortiz-Nieva, Fernando Fresán, Leonardo Villalvazo, Yonathan Garfias, Alejandro Navas, María C. Jiménez-Martínez

“…The patient was treated with keratolimbal allogenic transplantation and cryopreserved amniotic membrane in the right eye. Regardless of the treatment he persisted with torpid evolution developing retinal and choroidal detachments. …”
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First Autologous Cord Blood Therapy for Pediatric Ischemic Stroke and Cerebral Palsy Caused by Cephalic Molding during Birth: Individual Treatment with Mononuclear Cells by A. Jensen, E. Hamelmann

“…This patient was treated by autologous cord blood mononuclear cells (45.8 mL, cryopreserved, TNC 2.53×10e8) with a remarkable recovery. …”
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Human Amniotic Membrane Positioning in the Surgical Treatment of Temporomandibular Joint Degenerative Disorder by Luca Guarda-Nardini, Diletta Trojan, Giulia Montagner, Elisa Cogliati, Matteo Bendini, Daniele Manfredini

“…In the present case report, the authors describe the application of a cryopreserved HAM patch within the joint space as a disc-replacing film during major surgeries for discectomy and arthroplasty. …”
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Mesenchymal Stem Cells in Restoration of Fertility at Experimental Pelvic Inflammatory Disease by Nataliia Volkova, Mariia Yukhta, Anatoliy Goltsev

“…Based on this, we investigated the effect of intravenous administration of cryopreserved mesenchymal stem cells (cMSCs) of bone marrow on experimental chronic inflammation of the ovaries. …”
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Articular Cartilage Repair Using Marrow Stimulation Augmented with a Viable Chondral Allograft: 9-Month Postoperative Histological Evaluation by James K. Hoffman, Sandra Geraghty, Nicole M. Protzman

“…However, marrow stimulation typically results in fibrocartilage repair tissue rather than healthy hyaline cartilage, which, over time, predisposes the repair to failure. Recently, a cryopreserved viable chondral allograft was developed to augment marrow stimulation. …”
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First Autologous Cell Therapy of Cerebral Palsy Caused by Hypoxic-Ischemic Brain Damage in a Child after Cardiac Arrest—Individual Treatment with Cord Blood by A. Jensen, E. Hamelmann

“…Nine weeks later, he received autologous cord blood (91.7 mL, cryopreserved, 5.75×10e8 mononuclear cells) intravenously. …”
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Pilot Study: Human Adipose Tissue Allograft for Fat Pad Defects in Patients With Preulcerative Lesions by Matthew J. Regulski, DPM, Molly C. Saunders, BS, Sharron E. McCulloch, MS, Alla Danilkovitch, PhD

“…To address this, a human cryopreserved adipose tissue (hCAT) allograft has been developed to repair adipose tissue defects. …”
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Rationale and Design of the First Double-Blind, Placebo-Controlled Trial with Allogeneic Adipose Tissue-Derived Stromal Cell Therapy in Patients with Ischemic Heart Failure: A Phas... by Jens Kastrup, Morten Schou, Ida Gustafsson, Olav W. Nielsen, Rasmus Møgelvang, Klaus F. Kofoed, Charlotte Kragelund, Jens Dahlgaard Hove, Andreas Fabricius-Bjerre, Merete Heitman, Mandana Haack-Sørensen, Lisbeth Drozd Lund, Ellen Mønsted Johansen, Abbas Ali Qayyum, Anders Bruun Mathiasen, Annette Ekblond

“…It is produced without animal products, in closed bioreactor systems and cryopreserved as an off-the-shelf product ready to use. …”
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Mucosal leishmaniasis is associated with the Leishmania RNA virus and inappropriate cutaneous leishmaniasis treatment. by Fredy A Pazmiño, Marcela Parra-Muñoz, Carlos H Saavedra, Sandra Muvdi-Arenas, Clemencia Ovalle-Bracho, María C Echeverry

“…Clinical data were recorded from the patient's medical records. Cryopreserved biopsies were used to detect LRV1 and identify Leishmania species.…”
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Exploring the Most Promising Stem Cell Therapy in Liver Failure: A Systematic Review by Jeanne AdiwinataPawitan

“…Intravenous (IV) route was equal to invasive route. Fresh or cryopreserved, and autologous or allogeneic MSCs were equally beneficial; and giving too many cells via intraportal or the hepatic artery might be counterproductive.…”
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Effect of a single rectal fecal microbiota transplantation on clinical severity and fecal microbial communities in dogs with chronic inflammatory enteropathy by Jorge Pérez‐Accino, Mazdak Salavati, Laura Glendinning, Silke Salavati Schmitz

“…The effect of glycerol as a cryopreservative when storing FMT preparations also was assessed. …”
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Adipose Tissue-Derived Stromal Cells Induce a Highly Trophic Environment While Reducing Maturation of Monocyte-Derived Dendritic Cells by Morten Juhl, Bjarke Follin, Monika Gad, Jesper Larsen, Jens Kastrup, Annette Ekblond

“…ASCs were isolated from healthy voluntary donors, expanded using a human platelet lysate in bioreactors, and cryopreserved as per clinical use. Monocyte-derived dendritic cells were generated by isolation of monocytes and differentiation with GM-CSF and IL-4. …”
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Fixed timed artificial insemination in buffalo heifers with fresh and frozen-thawed semen in Argentina by José Luis Konrad, Alexis Bandeo, Natalia Vallejos, Pablo Ponce, Jesús Alfredo Berdugo, Pablo Maldonado-Vargas

“…The fertility of frozen-thawed semen was lower than that of frozen- thawed semen, without being significant, which should motivate veterinarians and breeders to cryopreserve semen from breeding stock. It is shown how acceptable pregnancy rates are obtained independently of the semen condition, which allows visualizing the application of artificial insemination in this species to achieve the necessary improvement to satisfy the needs of the industry. …”
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Safeguarding and using global banana diversity: a holistic approach by Ines Van den houwe, Rachel Chase, Julie Sardos, Max Ruas, Els Kempenaers, Valentin Guignon, Sebastien Massart, Sebastien Carpentier, Bart Panis, Mathieu Rouard, Nicolas Roux

“…Abstract The CGIAR genebank International Musa Germplasm Transit Centre (ITC) currently holds 1617 banana accessions from 38 countries as an in vitro collection, backed-up by a cryopreserved collection to safeguard global Musa diversity in perpetuity. …”
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Single-cell RNA sequencing highlights the role of distinct natural killer subsets in sporadic amyotrophic lateral sclerosis by Esther Álvarez-Sánchez, Álvaro Carbayo, Natalia Valle-Tamayo, Laia Muñoz, Joaquim Aumatell, Soraya Torres, Sara Rubio-Guerra, Jesús García-Castro, Judit Selma-González, Daniel Alcolea, Janina Turon-Sans, Alberto Lleó, Ignacio Illán-Gala, Juan Fortea, Ricard Rojas-García, Oriol Dols-Icardo

“…For this purpose, peripheral blood mononuclear cells (PBMC) were isolated from 14 ALS patients and 14 cognitively unimpaired healthy individuals (HC), matched by age and gender, and cryopreserved until library preparation and scRNAseq. …”
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Ovarian stimulation alternatives for in vitro production of embryos in water buffaloes by Alexis Bandeo, José Luis Konrad, Natalia Vallejos, Pablo Ponce, Marina J. Sansinena, Gustavo Crudeli, Pablo Maldonado-Vargas

“…In vitro fertilization (IVF) was performed after 24 hours of oocyte in vitro maturation using cryopreserved semen from buffalo bulls of proven fertility. …”
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Exploring the effect of cold stress on the semen quality parameters of buffalo bulls by Karpenahalli Ranganatha Sriranga, Pawan Singh, Ravinder Singh, Tejeshwari Satpute, Prince Vivek, Tushar Kumar Mohanty, Pradeep Kumar, Ranjit Singh Kataria, Megha Pande, Manishi Mukesh

“…., semen volume, sperm concentration, mass activity, individual motility, viability, acrosome integrity, and hypo-osmotic swelling test (HOST), were evaluated immediately after semen collection, and diluted semen was cryopreserved for further studies. Additionally, fresh and frozen sperm were subjected to flow cytometric analysis utilizing the Luminex MUSE Cell Analyzer and CytoFLEX, Beckman Coulter-Life Sciences, respectively. …”
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