Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates by Ligiane de Oliveira Leme, Mauricio Machaim Franco, Otávio Augusto de Faria, Alexandre Rodrigues Caetano, Juliana Gonçalves de Souza, Luciano de Rezende Carvalheira, Emivaldo de Siqueira Filho, Margot Alves Nunes Dode

“…Subsequently, we selected SOF25 for comparison with SOF100 in a commercial laboratory setting, evaluating embryo production, response to cryopreservation, gestation rate, and offspring birth. …”
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Cell Surface Proteins for Enrichment and In Vitro Characterization of Human Pluripotent Stem Cell-Derived Myogenic Progenitors by Sin-Ruow Tey, Madison Mueller, Megan Reilly, Colton Switalski, Samantha Robertson, Mariko Sakanaka-Yokoyama, Masatoshi Suzuki

“…We also demonstrated that cells in unsorted, CD271+, and CD15– fractions responded differently to cryopreservation and prolonged culture expansion. Lastly, we showed that CD271 expression is essential for terminal differentiation of human PSC-derived myogenic progenitors. …”
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REDERIVATION BY EMBRYO TRANSFER IN STRAINS OF LABORATORY MICE AND RATS by S. Ya. Amstislavsky, T. N. Igonina, I. N. Rozhkova, E. Yu. Brusentsev, A. A. Rogovaya, D. S. Ragaeva, V. A. Naprimerov, E. A. Litvinova, I. Z. Plyusnina, A. L. Markel

“…The optimized model of rederivation offered here involves a combination of such embryotechnological methods as freezing/cryopreservation of embryos, their washing through the number of fresh volumes of sterile media, growing in vitro for 48 hours, and subsequent transfer into either one or both uterine horns of recipient females. …”
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Correlation between Post-Thaw Spermatozoa Quality of the Endangered Javan Banteng with OPN Gene Expression by Vincentia Trisna Yoelinda, Raden Iis Arifiantini, Dedy Duryadi Solihin, Muhammad Agil, Dedi Rahmat Setiadi, Tulus Maulana, Bambang Purwantara, Yohana Tri Hastuti, Jansen Manansang, Dondin Sajuthi

“…The development of semen cryopreservation may assist on one side of the conservation plan. …”
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In situ-formed cryomicroneedles for intradermal cell delivery by Mengjia Zheng, Tianli Hu, Yating Yang, Xuan Qie, Huaxin Yang, Yuyue Zhang, Qizheng Zhang, Ken-Tye Yong, Wei Liu, Chenjie Xu

“…Specifically, S-cryoMNs are made by dipping a porous MN scaffold in the cell suspension before cryopreservation. The porous scaffold can be transported at room temperature, and researchers can load any cells with the optimized cryogenic medium. …”
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The Sarcoplasmic/Endoplasmic reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation by Ferran Garriga, Jesús Martínez-Hernández, Ainhoa Parra-Balaguer, Marc Llavanera, Marc Yeste

“…Abstract Liquid storage is the primary preservation method in the swine breeding industry because of its advantages over cryopreservation. Calcium (Ca2+), a key regulator of cell physiology, plays a crucial role during liquid preservation. …”
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Applying reproductive technologies and genome resource banking to laboratory animals by S. Ya. Amstislavsky, E. Yu. Brusentsev, T. O. Abramova, D. S. Ragaeva, I. N. Rozhkova, T. N. Igonina, E. A. Kizilova, V. A. Naprimerov, N. Yu. Feoktistoiva

“…Some other laboratory animal species have been cryopreserved as well. Embryos of two hamster species (Djungarian and Campbell’s) in the genus Phodopus were cryopreserved and the viability of thawed embryos was proved by their successful development in vitro and in vivo (by transfer to a recipient). …”
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Immunomodulatory Effects of 1,25-Dihydroxyvitamin D3 on Dendritic Cells Promote Induction of T Cell Hyporesponsiveness to Myelin-Derived Antigens by Wai-Ping Lee, Barbara Willekens, Patrick Cras, Herman Goossens, Eva Martínez-Cáceres, Zwi N. Berneman, Nathalie Cools

“…Furthermore, the feasibility to cryopreserve highly potent tolDC will, ultimately, contribute to the large-scale production and the widely applicable use of tolDC.…”
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Intratesticular Angiolipoma: A Rare Case of Adipose Tissue Presence in the Testis by V. Kalyvas, C. Gkekas, D. Papadopoulos, A. Malioris, S. Milias, M. Papathanasiou, N. Kalinderis, K. Moysidis, K. Hatzimouratidis

“…After sonographic and MRI investigation, the patient was referred for semen analysis and cryopreservation. Subsequently, the patient underwent testicular biopsy (frozen section biopsy) and right partial orchiectomy. …”
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Exploring the need for fertility education: A cross-sectional study of healthcare professionals and students’ knowledge of fertility and fertility preservation by Ana GALHARDO, Catarina SALVADO, Soraia MONIZ, Marina Cunha, Ilda MASSANO-CARDOSO

“…Participants' answers regarding factors affecting fertility showed that more than half of the participants, more than half of the students (54.6%), and the professionals (60%) overestimated the age of women’s fertility decline. Oocyte cryopreservation was the fertility preservation option they knew best. …”
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Proteomics and metabolomics analyses of mechanism underlying bovine sperm cryoinjury by Renzheng Zhang, Xiuyuan Wang, Ruili Liu, Yanfang Mei, Xiuping Miao, Jiaxu Ma, Lei Zou, Qiuyue Zhao, Xuejin Bai, Yajuan Dong

“…Abstract Background The cryoinjury of semen during cryopreservation reduces sperm motility, constraining the application of artificial insemination (AI) in bovine reproduction. …”
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Metabolic phenotype of adult mice offspring obtained from different variants of embryo transfer by M. V. Anisimova, Ya. Gong, N. S. Yudin, Yu. M. Moshkin, L. A. Gerlinskaya

“…In the management of collections of genetic lines of laboratory animals, these technologies are obligatory components of cryopreservation and rederivation. ART procedures include the development of early embryos outside the mother’s body and the high probability of incomplete synchronization of the physiological state of the surrogate mother and transplanted embryos. …”
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Controlled ovarian hyperstimulation with or without letrozole for fertility preservation in breast cancer patients: study protocol for a randomised controlled trial by Ping Li, Kun Wang, Qi Fan, Peng Sun, Yuhua Shi, Yanqiu Xie, Weifen Deng, Jiajing Kang

“…The standard options for fertility preservation are oocyte and embryo cryopreservation; both require controlled ovarian hyperstimulation (COH). …”
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Studies of biological properties of continuous suspension ВНК-21/SUSP/ARRIAH cell line by M. I. Doronin, M. N. Guseva, D. V. Mikhalishin, A. S. Sharypov, N. S. Mudrak, N. Ye. Kamalova, B. L. Manin

“…BHK-21/SUSP/ARRIAH cells recover rapidly after cryopreservation and demonstrate 95–99% viability and growth rate of 3.36–5.88 at passages 1 to 3 and 6.85–10.95 at passages 4 to 12. …”
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Germ cell quantification in human fetal and prepubertal testis tissues: a comparison of current methodologies by Emma Kearney, David Greenald, Gabriele Matilionyte, Sheila Lane, Melissa D Tharmalingam, Jill Davies, Jan-Bernd Stukenborg, Grace Forsyth, Rod T Mitchell

“…Human testicular samples included fetal tissue (exposed in vitro to cisplatin, carboplatin or control) or prepubertal tissue (fresh, cryopreserved, fresh in vitro cultured or cryopreserved in vitro cultured). …”
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The adaptation of rainbow trout to warmer water: Oxidative damage in the germinal line by Vianel Sevastei, Sonia A. Crichigno, M. Victoria Santos, Andrea Trochine, Julio C. Painefilú, Noemí Zaritzky, Víctor E. Cussac

“…Semen fatty acid composition, sperm morphology, sperm motility, and fertilization performance in samples before and after the application of cryopreservation procedures were also evaluated. The observed responses, mainly related to the sperm membrane, reinforces the idea that ROS can affect the germinal line of male rainbow trout juveniles subjected to high water temperature. …”
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Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion and drug screening by Anand Ramani, Giovanni Pasquini, Niklas J. Gerkau, Vaibhav Jadhav, Omkar Suhas Vinchure, Nazlican Altinisik, Hannes Windoffer, Sarah Muller, Ina Rothenaigner, Sean Lin, Aruljothi Mariappan, Dhanasekaran Rathinam, Ali Mirsaidi, Olivier Goureau, Lucia Ricci-Vitiani, Quintino Giorgio D’Alessandris, Bernd Wollnik, Alysson Muotri, Limor Freifeld, Nathalie Jurisch-Yaksi, Roberto Pallini, Christine R. Rose, Volker Busskamp, Elke Gabriel, Kamyar Hadian, Jay Gopalakrishnan

“…These High Quantity brain organoids (Hi-Q brain organoids) exhibit reproducible cytoarchitecture, cell diversity, and functionality, are free from ectopically active cellular stress pathways, and allow cryopreservation and re-culturing. Patient-derived Hi-Q brain organoids recapitulate distinct forms of developmental defects: primary microcephaly due to a mutation in CDK5RAP2 and progeria-associated defects of Cockayne syndrome. …”
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Malunion of the Acetabulum Treated by Intra-articular Buttress by D. Saragaglia, J. Gaillot

“…We treated a protruding acetabular malunion, 20 years ago, using an intra-articular buttress, by means of an allograft corresponding to a femoral head fragment which had been cryopreserved. The radiological and clinical result with this extended follow-up is quite remarkable, which has motivated us to present this original technique.…”
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PATIENTS' CHRONOTYPE INFLUENCE IN TREATMENT EFFICIENCY OF PLACENTA CRYERXTRACT CHANGES IN MAXILLOFACIAL PHLEGMONS by K. Lokes, M. Pérez Sayáns, V. Lychman, D. Steblovskyi, I. Boiko, D. Avetikov

“…Analyzing the obtained data, it can be asserted that the most significant changes in markers of lipid peroxidation in patients with superficial phlegmons of maxillofacial localization were experienced in both experimental groups when used against the background of treatment according to standard protocols of intravenous injections of the cryopreserved placenta without and in combination with local injection of the cryopreserved placenta as part of "Levomekol" ointment, in contrast to the control group. …”
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Protocol for a patient-derived preclinical platform to model tumor-immune interactions and evaluate therapeutic efficacy by Wen-Hsin Chang, Andrew I. Chin, Ching-Hsien Chen

“…We outline steps for isolating human peripheral blood mononuclear cells (PBMCs), preparing patient-derived precision-cut tumor slices (PCTSs), cryopreserving the samples, and setting up the co-culture system. …”
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