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81
Direct measurement of the critical cooling rate for the vitrification of water
Published 2025-01-01“…The vitrification of aqueous solutions through rapid cooling is a remarkable achievement that launched the field of cryo-electron microscopy (cryo-EM) and has enabled the cryopreservation of biological specimens. For judging the feasibility of a vitrification experiment, the critical cooling rate of pure water is a frequently cited reference quantity. …”
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82
Study of the Boson Peak and Fragility of Bioprotectant Glass-Forming Mixtures by Neutron Scattering
Published 2013-01-01“…In addition, these systems are employed in different industrial fields, such as pharmaceutical and cosmetic industries, as additives in mixtures for cryopreservation and in several formulations. This review article shows an overview of Inelastic Neutron Scattering (INS) data, collected at different temperature values by the OSIRIS time-of-flight spectrometer at the ISIS Facility (Rutherford Appleton Laboratory, Oxford, UK) and by the IN4 and IN6 spectrometers at the Institut Laue Langevin (ILL, Grenoble, France), on trehalose/glycerol mixtures as a function of the glycerol content. …”
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83
Safety of Ovarian Tissue Autotransplantation for Cancer Patients
Published 2012-01-01“…Cancer treatments can induce premature ovarian failure in almost half of young women suffering from invasive neoplasia. Cryopreservation of ovarian cortex and subsequent autotransplantation of frozen-thawed tissue have emerged as promising alternatives to conventional fertility preservation technologies. …”
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84
Reproductive Late Effects in Female Survivors of Childhood Cancer
Published 2012-01-01“…Fertility preservation options including cryopreservation, oophoropexy, and gonadotropin-releasing hormone analogues may be initiated prior to treatment, although most are still considered experimental. …”
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85
Cancer, Fertility Preservation, and Future Pregnancy: A Comprehensive Review
Published 2012-01-01“…Recent advances in oocyte cryopreservation and ovarian stimulation protocols will be discussed. …”
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86
Regional Variation in Rates of IVF Treatment across Australia: A Population-based Study
Published 2017-03-01“…**Conclusion:** These findings suggest that factors such as physician preference, clinical practice guidelines, and cryopreservation protocols of ART units might explain the national variation in uptake of IVF.…”
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87
Stem Cell Interaction with Somatic Niche May Hold the Key to Fertility Restoration in Cancer Patients
Published 2012-01-01“…The spontaneous return of fertility after bone marrow transplantation or heterotopic grafting of cryopreserved ovarian cortical tissue has surprised many, and a possible link with stem cells has been proposed. …”
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88
Development of the myocite biobank: Cost-efficient model of public sector investigator-driven biobank for idiopathic inflammatory myositis
Published 2020-01-01“…Background: Biobanking refers to the cryopreservation of the various biologic samples for future research. …”
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89
Oocyte Cryostorage to Preserve Fertility in Oncological Patients
Published 2012-01-01“…Several techniques have been proposed to preserve fertility in these patients; among them oocyte collection and cryopreservation prior to the oncostatic treatment has been widely applied in the last decade. …”
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90
Reproductive performance and increased fecundity in the chinchilla (Chinchilla laniger Molina)
Published 2015-07-01“…To improve the fecundity of chinchillas, use of reproductive technologies is recommended, including electroejaculation, sperm cryopreservation, estrus induction, hormonal stimulation of ovulation, and others. …”
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91
The UK Crop Microbiome Cryobank: a utility and model for supporting Phytobiomes research
Published 2023-11-01“…This manuscript describes the approaches being taken, from characterisation, cryopreservation and analysis of the crop microbiome through to potential applications. …”
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92
Trans-Vessel Wall Cell Transplantation, Engraftment, and Tumor Access in the VX2 Rabbit Model
Published 2025-01-01“…We used the VX2 model in the rabbit kidney to compare percutaneously implanted fresh VX2 cells with TW-device injections of cryopreserved VX2 cells. We demonstrated the feasibility of endovascular transplantation ( n = 7) of tumor cells, achieving a 57.1% engraftment rate despite cryopreservation, comparable with 70% for percutaneous delivery of fresh cells ( n = 10). …”
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93
Effect of mesenchymal stem cells on animal semen during storage
Published 2023-12-01“…Therefore, possibility of using the MSCs for the storage and cryopreservation of the semen of some animals was demonstrated, but this requires further research.…”
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94
Stem Cell-Based Therapies for Liver Diseases: State of the Art and New Perspectives
Published 2010-01-01“…The evaluation of its efficacy is complicated by the shortage of human hepatocytes and their difficult expansion and cryopreservation. Recent advances in cell biology have led to the concept of “regenerative medicine”, based on the therapeutic potential of stem cells (SCs). …”
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95
Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication
Published 2012-01-01“…In 83 of these 177 oocytes, the spindle was observed to be in the same location as it was before cryopreservation (46.9%). However, in 94 of these 177 oocytes (53.1%), the spindle reformed in a different position/location relative to the polar body. …”
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96
Somatic embryogenesis in Larix: the state of art and perspectives
Published 2020-10-01“…In combination with cryopreservation, somatic embryogenesis creates the basis for the development of economically valuable lines of clones and elite genotypes. …”
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97
TRANSMETACARPAL REPLANTATION. ELEVEN YEARS LATER
Published 2024-03-01“…During replantation enormous tissue edema appeared, caused by long ischemia time with cryopreservation of the amputated part for three hours. …”
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98
Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy
Published 2025-01-01“…Although human induced pluripotent stem cell (iPSC)-derived models have advanced NMJ research, their application is constrained by challenges such as limited differentiation efficiency, lengthy generation times, and cryopreservation difficulties. To overcome these limitations, we developed a rapid human NMJ model using cryopreserved MNs and SKMs derived from iPSCs. …”
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99
Protocol for differentiating cardiomyocytes and generating engineered heart tissues from human feeder-free extended pluripotent stem cells
Published 2025-03-01“…We describe steps for converting human embryonic stem cells or induced pluripotent stem cells (ESCs/iPSCs) into ffEPSCs, followed by their long-term maintenance, cryopreservation, seed preservation, and differentiation into cardiomyocytes. …”
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100
Microbiological tests of fresh bull semen collected at breeding establishment
Published 2022-12-01“…Since antimicrobials contained in the diluent used during semen product cryopreservation do not allow an objective assessment of semen contamination, a microbiological testing of fresh undiluted bull semen was carried out at the AO «Krasnoyarskagroplem» breeding establishment to identify the contamination source. …”
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