Enhanced Expression and Bioactivity Assessment of Recombinant SUMO-Protease-1 in E. coli BL21 (DE3) via Cleavage of His6-SMT3-SDF-1 Fusion Protein by Farzad Sadri, Hossain Safarpour, Zahra Ansari, Yazdan Ebrahimpour, Mohammad Feriedouni

“…SUMO-protease-1 (Ulp1) is a key enzyme in this system, and its proper expression and purification in Escherichia coli BL21 (DE3) are critical to its efficiency. Objective. To optimize Ulp1 expression and purification using His6-SUMO-SDF-1 as a model, aiming to improve efficiency across similar fusion proteins. …”
Get full text

Legal consciousness as a prerequisite for person’s lawful behavior: retrospective view, modern interpretation by А. М. Shulha

“…It has been noted that in today’s conditions the term “legal consciousness” is understood broadly, it covers, which is paradoxical or insufficiently correct from a critical point of view, both conscious and unconscious in the legal sphere of the inner world of man, that is, there is a need to distinguish between the concepts of “legal consciousness” and “legal subconsciousness”. …”
Get full text

Species specificity and specificity diversity (SSD) framework: a novel method for detecting the unique and enriched species associated with disease by leveraging the microbiome het... by Zhanshan Sam Ma

“…Abstract Background Differentiating the microbiome changes associated with diseases is challenging but critically important. Majority of existing efforts have been focused on a community level, but the discerning power of community or holistic metrics such as diversity analysis seems limited. …”
Get full text

Oral health status, behavior and impact profile among naval personnel in the Malaysian naval armed forces by Nik Madihah Nik Azis, Raja Noreihan Raja Abdullah, Mohamad Nur Adzmi Mohamad Ayub, Badiah Baharin

“…Abstract Background The oral health of military personnel is critical in ensuring their operational readiness and overall health. …”
Get full text

Evaluation of the effect of dental caries, oral hygiene, and treatment need on oral health-related quality of life among Turkish orphan children and adolescents by Ecem Akbeyaz Şivet, Berkant Sezer, Neslihan Atmaca, Narmin Hüseynova, Betül Kargül

“…Abstract Background The oral and dental health and related quality of life of orphan children and adolescents—who are considered a vulnerable population—are critical for both individual and public health. Objectives This study aimed to investigate the status of the dental caries, oral hygiene, and dental treatment needs among a group of Turkish male orphan children and adolescents, as well as their impact on oral health-related quality of life. …”
Get full text

Genetic gain of grain yield and quality in bread wheat cultivars representing 40 years of breeding in Morocco by S. Bennani, M. Taghouti, F. Gaboun

“…Knowledge about the genetic gain for fundamental traits over time is essential for a critical assessment and improvement of breeding programs, especially regarding staple crops like bread wheat.Materials and methods. …”
Get full text

Development and performance evaluation of sustainable lightweight geopolymer based fireproofing coatings for steel construction by Zhenyu Huang, Yingwu Zhou, Hammad Salahuddin

“…This study developed a novel, sustainable, lightweight, and high-ductility fireproofing coating using granulated blast furnace slag (GBFS), fly ash microspheres (FAC), alkali activator, and polyethylene (PE) fiber as raw materials. The critical mixing ratios of FAC to GBFS (4:6), the water-to-binder ratio (0.55), and the alkali activator modulus (1.4) were determined to meet the requirements for fluidity, compressive strength, and flexural strength. …”
Get full text

Assessment of ecological resilience and its response mechanism to land spatial structure conflicts in China’s Southeast Coastal Areas by Yuemin Fan, Guoen Wei

“…Consequently, assessing the impact of land spatial structural transformation on environmental resilience and developing suitable regulatory strategies has emerged as a critical issue in this region. This study develops an assessment framework for ecological resilience (ER) and “Production-Living- Ecological” space structure conflict (SCCI) utilizing multi-source remote sensing data and socioeconomic statistics from 2000 to 2022. …”
Get full text

Comparing the performance of ChatGPT and ERNIE Bot in answering questions regarding liver cancer interventional radiology in Chinese and English contexts: A comparative study by Xue-ting Yuan, Chen-ye Shao, Zhen-zhen Zhang, Duo Qian

“…Introduction This study aims to critically assess the appropriateness and limitations of two prominent large language models (LLMs), enhanced representation through knowledge integration (ERNIE Bot) and chat generative pre-trained transformer (ChatGPT), in answering questions about liver cancer interventional radiology. …”
Get full text

Improving Crack Detection Precision of Concrete Structures Using U-Net Architecture and Novel DBCE Loss Function by Andrew Prasetyo, I Ketut Eddy Purnama, Eko Mulyanto Yuniarno, Priyo Suprobo

“…Monitoring the health of infrastructure is critical to maintaining the integrity of concrete construction. …”
Get full text

Emerging Strategies in Cartilage Repair and Joint Preservation by Mircea Adrian Focsa, Sorin Florescu, Armand Gogulescu

“…<i>Background and Objectives:</i> Cartilage repair remains a critical challenge in orthopaedic medicine due to the tissue’s limited self-healing ability, contributing to degenerative joint conditions such as osteoarthritis (OA). …”
Get full text

Progress in the Management of Retinal Detachment Associated With Morning Glory Syndrome by Wu S, Wang W, Liu L, Wang W, Jiang K, Peng C, Sun H, Zhang J

“…The etiology of MGS is not fully understood but has been associated with genetic factors, particularly mutations in the PAX6 and PAX2 genes, which play critical roles in eye development. MGS is often linked with other ocular and systemic anomalies, including retinal detachment, a significant complication that may be exudative, tractional, or rhegmatogenous. …”
Get full text

Enhanced precision in stone localization and intraoral removal in sialolithiasis: the role of preoperative computer tomographic scanning in surgical planning by Soo Yeon Jung, Mi Sun Chun, Yu Jin Go, Ju Hyun Yun, Han Su Kim

“…T-test was performed to compare stone characteristics between different locations, and a receiver operating characteristic curve analysis was used to identify the critical size threshold for predicting stone location. …”
Get full text

Effectiveness and safety of herbal medicines for induction of labour: a systematic review and meta-analysis by Edward Fottrell, Carina King, Chrispin Mandiwa, Collins Zamawe, Hannah Maria Jennings

“…The risk of bias was assessed using ‘John Hopkins Nursing School Critical Appraisal Tool’ and ‘Cochrane Risk of Bias Tool’.Results A total of 1421 papers were identified through the searches, but only 10 were retained after eligibility and risk of bias assessments. …”
Get full text

The evaluation of the predictors of left ventricular systolic function improvement in patients with severe aortic stenosis after aortic valve replacement by N.V. Ponych, O.J. Zharinov, O.A. Yepanchintseva, B.M. Todurov

“…Under proper selection of patients with AS and reduced LVEF for surgery more than 30 % improvement of LVEF may be expected at early postoperative period. Critical AS with reduced LVEF, including low-flow, low gradient AS should not be regarded as an independent restriction to AVR.…”
Get full text

DisCo P-ad: Distance-Correlation-Based <i><b>p</b></i>-Value Adjustment Enhances Multiple Testing Corrections for Metabolomics by Debmalya Nandy, Debashis Ghosh, Katerina Kechris

“…Therefore, correcting for multiple testing is critical while performing any statistical test of significance to minimize the chances of false or missed discoveries. …”
Get full text

Effects of Land Uses on Soil Quality Indicators: The Case of Geshy Subcatchment, Gojeb River Catchment, Ethiopia by Melku Dagnachew, Awdenegest Moges, Asfaw Kebede Kassa

“…Land degradation caused by improper land use management is a critical worldwide problem that has revived the issue of resources sustainability. …”
Get full text

Genesis of the Longmendian Ag–Pb–Zn Deposit in Henan (Central China): Constraints from Fluid Inclusions and H–C–O–S–Pb Isotopes by Xinglin Chen, Yongjun Shao, Chunkit Lai, Cheng Wang

“…The fluid boiling and cooling and mixing with meteoric water may have been critical for the Ag–Pb–Zn ore precipitation. Geological and geochemical characteristics of the Longmendian deposit indicate that the deposit is best classified as medium- to low-temperature intermediate-sulfidation (LS/IS) epithermal-type, related to Cretaceous crustal-extension-related granitic magmatism.…”
Get full text

Hepatoprotective Role of 4-Octyl Itaconate in Concanavalin A-Induced Autoimmune Hepatitis by Wenchang Yang, Yaxin Wang, Peng Zhang, Tao Wang, Chengguo Li, Xin Tong, Xiangyu Zeng, Yuping Yin, Kaixiong Tao, Ruidong Li

Get full text

The Golden Afromontane ecosystems at cross-road: Wetland-based upstream-downstream linkage in Genale Dawa River Basin by Habtamu Temesgen, Haile Ketema, Gezahegn Kebede, Fikadu Woldemariyam, Gebremedihin Tesfaye

“…This situation has placed the basin at risk and at a critical crossroads. Notably, the upstream landscape is now more exposed to anthropogenic pressures than in the past, with clear impacts observed both locally and downstream. …”
Get full text