A Novel, Patient-Specific Mathematical Pathology Approach for Assessment of Surgical Volume: Application to Ductal Carcinoma in situ of The Breast by Mary E. Edgerton, Yao-Li Chuang, Paul Macklin, Wei Yang, Elaine L. Bearer, Vittorio Cristini

“…We tested this idea in a retrospective study of 17 patients by staining breast tumor specimens containing ductal carcinoma in situ for mitosis with Ki-67 and for apoptosis with cleaved caspase-3 and counting cells positive for each marker. We also determined diffusion penetration by measuring the thickness of viable rims of tumor cells within ducts. …”
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Strengthening identification and characterization of causes of perinatal deaths in Kaski district of Nepal (Perinatal MITS Nepal) by Nuwadatta Subedi, Sunita Ranabhat, Sanjib Mani Regmi, Mukesh Mallik, Dela Singh, Shree Krishna Shrestha, Bandana Gurung, Arjun Bhattarai, Madan Prasad Baral, Sudhir Raman Parajuli, Ramchandra Bastola, Junu Shrestha, Sahisnuta Basnet, Eva Gauchan, Sabita Paudel

“…Discussion The mechanism of counting and accounting for deaths in a systematic manner is important and it can provide evidence to determine changes in clinical practice and to develop guidelines and training packages for preventive measures. …”
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EIF4A3-induced circ_0022382 promotes breast cancer cell progression through the let-7a-5p/PI3K/AKT/mTOR signaling pathway and SLC7A11 axis by Wei Liu, Wei Liu, Jun Zhang, Jiawen Zhang, Yu Ye, Jianqin Zhu, Qiwen Yu, Tao Li, Tao Li, Xiaochun Sun, Huabiao Chen, Huabiao Chen

“…In this study, we explored the impacts of circRNAs on the progression of breast cancer cells.MethodsUsing bioinformatics analysis, we screened out one up-regulated circRNA in breast cancer, and its function and regulatory mechanisms were confirmed by quantitative real-time PCR, cell counting kit-8 experiment, migration assay, dual luciferase reporter assay, Kyoto Encyclopedia of Genes and Genomes enrichment analysis, cell immunofluorescence, clone formation assay, scratch wound healing experiment, RNA immunoprecipitation and subcutaneous tumor-bearing experiments.ResultsCirc_0022382 was highly expressed in breast cancer cell lines MDA-MB-231, MCF-7 as well as breast cancer tissues, and promoted the proliferative and migratory capacity of breast cancer cells. …”
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Polyphenols and pectin enriched golden kiwifruit (Actinidia chinensis) alleviates high fructose-induced glucolipid disorders and hepatic oxidative damage in rats: in association wi... by Aamina Alim, Ting Li, Tanzeela Nisar, Zeshan Ali, Daoyuan Ren, Yueyue Liu, Xingbin Yang

“…This study delivers a scientific foundation for developing kiwifruit (counting peel)-based dietary supplements for those with glucolipid-metabolic disorders and liver damage.…”
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Temporal Changes in Plasmodium Falciparum Genetic Diversity and Multiplicity of Infection Across Three Areas of Varying Malaria Transmission Intensities in Uganda. by Mwesigwa, Alex, Kiwuwa, Steven M., Musinguzi, Benson, Kawalya, Hakiim, Katumba, James Davis, Baguma, Andrew, Mutuku, Irene M., Adebayo, Ismail Abiola, Nsobya, Samuel L., Byakika‐Kibwika, Pauline, Kalyango, Joan N., Karamagi, Charles, Nankabirwa, Joaniter I.

“…MOI was assessed by counting distinct genotypes. Multi-locus linkage disequilibrium (LD) and genetic differentiation were evaluated using the standardized index of association ( IAS) and Wright’s fixation index (FST), respectively. …”
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Relationship between CTF1 gene expression and prognosis and tumor immune microenvironment in glioma by Hongqing Cai, Shen Tian, Angsi Liu, Guanchao Xie, Hongsheng Zhang, Xiaogang Wu, Jinghai Wan, Sai Li

“…To assess the impact on glioma cell proliferation and apoptosis, we employed various assays, including the Cell Counting Kit-8, colony formation assay, and flow cytometry. …”
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Exploring the impact of insufficient thermal ablation on hepatocellular carcinoma: NDST2 overexpression mechanism and its role in facilitating growth and invasion of residual cance... by Weijun Wan, Danxia Guo, Tong Kang, Jinshu Pang, Yunjing Pan, Jiamin Chen, Wei Liao, Yuji Chen, Peng Lin, Lipeng Li, Hong Yang, Yun He

“…Additionally, NDST2 was knocked down in heat stress-induced HCC cells, and the effects of NDST2 on these cells were verified using Cell Counting Kit-8 assays, scratch assays, and Transwell assays.Results NDST2 expression levels are elevated in HCC, leading to a decrease in overall survival rates of HCC patients. …”
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Iguratimod Inhibits the Aggressiveness of Rheumatoid Fibroblast-Like Synoviocytes by Jin Lin, Ye Yu, Xuanwei Wang, Yini Ke, Chuanyin Sun, Lihuan Yue, Guanhua Xu, Bei Xu, Liqin Xu, Heng Cao, Danyi Xu, Nancy Olsen, Weiqian Chen

“…The proliferation of RA-FLSs was assessed by 5-ethynyl-2′-deoxyuridine test and Cell Counting Kit-8. Migration and invasion were determined by the wound test and a transwell assay. …”
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Effect of miR-144-3p-Targeted Regulation of PTEN on Proliferation, Apoptosis, and Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells under Stretch by Shiyong Ling, Xi Luo, Bo Lv, Hua Wang, Mengzhi Xie, Kai Huang, Jingchuan Sun

“…The cell proliferation was detected by Cell Counting Kit-8 (CCK-8) colorimetric assay; the expression levels of cyclin, cyclin-dependent kinases (CDK), BCL2-associated X (BAX), B-cell lymphoma-2 (BCL-2), and other cell cycle and apoptosis related proteins were detected by western blot (WB); and the osteogenic differentiation ability of MSC cells was detected by ALP staining and alizarin red staining. …”
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Framework for smartphone-based grape detection and vineyard management using UAV-trained AI by Sergio Vélez, Mar Ariza-Sentís, Mario Triviño, Antonio Carlos Cob-Parro, Miquel Mila, João Valente

“…Viticulture benefits significantly from rapid grape bunch identification and counting, enhancing yield and quality. Recent technological and machine learning advancements, particularly in deep learning, have provided the tools necessary to create more efficient, automated processes that significantly reduce the time and effort required for these tasks. …”
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Olfactory mucosa-mesenchymal stem cells with overexpressed Nrf2 modulate angiogenesis and exert anti-inflammation effect in an in vitro traumatic brain injury model by Zigui Chen, Chunyuan Zhang, Yuhua Fang, He Zhang, Jiawei Luo, Changfeng Miao, Jiale Li, Jun Peng, Yingqi Qiu, Ying Xia, Qisheng Luo

“…The effects of OM-MSCsNrf2 on the survival and angiogenesis of PC12 cells were evaluated through cell counting kit-8 (CCK-8) and tube formation assay, and extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) were calculated to reflect glycolysis. …”
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Exploring the role of ELOVLs family in lung adenocarcinoma based on bioinformatic analysis and experimental validation by Zihan Wang, Wenjing Cui, Long Liang, Jingge Qu, Yuqiang Pei, Danyang Li, Ying Luo, Yue Zhang, Yifan Qiu, Yongchang Sun

“…Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis, cell counting kit-8 (CCK8), colony formation, wound healing, transwell migration assays and fatty acid metabolism detection were employed to confirm the significant role of ELOVL6 in vitro experiment. …”
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Diagnóstico de partida para diseñar un modelo de proyecto que permita implantar el proceso de inteligencia empresarial en las empresas del grupo de diseño e ingeniería de la constr... by Julio García, José A Macías

“…From these elements, an on-going scientific investigation design was created, counting with the co-sponsorship from several universities and companies. …”
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Molecular Mechanisms of Synergistic Effect of PRIMA‐1met and Oxaliplatin in Colorectal Cancer With Different p53 Status by Xiao‐lan Li, Jianbiao Zhou, Nicole Xin‐Ning Tang, Yi Chai, Meng Zhou, Ai‐di Gao, Zhong‐kai Lu, Han Min

“…Methods Cell viability was assessed with Cell Counting Kit‐8 (CCK‐8) assay and combination index (CI) was calculated using The Chou‐Talalay method. …”
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Upregulated SAE1 Drives Tumorigenesis and Is Associated with Poor Clinical Outcomes in Breast Cancer by Hong Liu, Jing Wang, Yunhai Li, Feng Luo, Lei Xing

“…BC cell proliferation was detected with the Cell Counting Kit-8 and by the colony formation assay. Cell cycle progression was analyzed by flow cytometry, and the expression of cell cycle-related proteins (E2F1, cyclin D3, and cyclin-dependent kinase 2) was determined by western blots in SAE1 small interfering RNA (siRNA) transfected cells. …”
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Toward Sustainable Biocultural Ecotourism: An Integrated Spatial Analysis of Cultural and Biodiversity Richness in Colombia by Alejandra Echeverri, Natasha M. Batista, Stacie Wolny, Guido A. Herrera‐R, Federico Andrade‐Rivas, Allison Bailey, Anaid Cardenas‐Navarrete, Armando Dávila Arenas, Andres Felipe Díaz‐Salazar, Katherine Victoria Hernandez, Kelley E. Langhans, Bryam E. Mateus‐Aguilar, Dallas Levey, Andrew Neill, Oliver Nguyen, Andres Felipe Suárez‐Castro, Felipe Zapata, Natalia Ocampo‐Peñuela

“…Using Colombia as a case study, we created metrics of taxonomic biological diversity as measured by vertebrate species richness (including birds, mammals, freshwater fishes, reptiles and amphibians) and institutionalized cultural richness (by counting the number of UNESCO World Heritage Sites, UNESCO Intangible Cultural Heritage sites, museums, endemic music festivals, Afro‐Colombian Territories and Indigenous Reserves) and evaluated the spatial correlations between them. …”
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High Ki67 expression, HER2 overexpression, and low progesterone receptor levels in high-grade DCIS: significant associations with clinical practice implications by Hossein Schandiz, Hossein Schandiz, Lorant Farkas, Lorant Farkas, Daehoon Park, Yan Liu, Yan Liu, Solveig N. Andersen, Torill Sauer, Jürgen Geisler, Jürgen Geisler

“…Low PR combined with high Ki67% was significantly associated with HER2 overexpression (p = 0.0107).ConclusionsInterobserver agreement for the Ki67 count was moderate. Ki67 expression showed considerable variability in high-grade DCIS. …”
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Uranium Mineral Transport in the Peña Blanca Desert: Dissolution or Fragmentation? Simulation in Sediment Column Systems by Victoria Pérez-Reyes, Rocio M. Cabral-Lares, Jesús G. Canche-Tello, Marusia Rentería-Villalobos, Guillermo González-Sánchez, Blanca P. Carmona-Lara, Cristina Hernández-Herrera, Fabián Faudoa-Gómez, Yair Rodríguez-Guerra, Gregorio Vázquez-Olvera, Jorge Carrillo-Flores, Ignacio A. Reyes-Cortés, Daniel Hernández-Cruz, René Loredo-Portales, María E. Montero-Cabrera

“…This study presents an experimental simulation of uranium transport in SPB sediments using three approaches: (i) a batch experiment to evaluate the ideal adsorption of (UO₂)²⁺ by fine sediment; (ii) a column system fed with 569 mgU L⁻¹ UO₂(NO₃)₂ to simulate adsorption by different sediment particle sizes; (iii) a column system with an upper horizon of uranophane from the area, fed with deionized water, to simulate uranium weathering and transport in particulate material, determined by liquid scintillation counting, revealed that the clay fraction had the highest adsorption capacity for U. …”
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Methyltransferase DNMT3B promotes colorectal cancer cell proliferation by inhibiting PLCG2 by Ji Yong, Wang Yang, Zou Jiacheng, Liu Guanghao, Xia Mingyu, Ren Jun, Wang Daorong

“…Cell Counting Kit-8 (CCK-8) and colony formation assays are used to assess the proliferation of colorectal cancer cells. …”
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Serum NLR combined with CA125 and HE4 improves the diagnostic and prognostic efficiency in patients with ovarian cancer by Yun Tian, Yun Tian, Xiabing Li, Xiabing Li, Hongjian Zhang, Hongjian Zhang, Yaping Wang, Yaping Wang, Hongyu Li, Hongyu Li, Qiaohong Qin, Qiaohong Qin

“…This study seeks to identify combination diagnostic and prognostic markers for OC, aiming to improve diagnostic and prognostic efficacy, especially in the early stages.MethodsWe analyzed the targeted biomarkers in a cohort of 104 OC patients and 100 controls, which comprised 50 patients with benign ovarian tumors and 50 healthy women, using enzyme-linked immunosorbent assay (ELISA) and complete blood counting (CBC). After validating the biomarker panel, we compared the expression levels of the biomarkers in OC patients with various clinical features to assess their relevance. …”
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