Immune-related gene SOX10 affects ferroptosis in pancreatic cancer and facilitates tumor progression by targeting CMTM7-mediated Wnt/β-catenin signaling pathway by Guixing Jiang, Bicheng Wu, Kaikai Wang, Xiaofan Pu, Senhao Zhou, Xin Zhong, Xiaolong Liu, Suihan Wang, Tianyu Lin

“…To assess cell functions, cell counting kit-8, flow cytometry, scratch test, and Transwell assays were performed. …”
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Impact of a new case-based payment scheme on volume distribution across public hospitals in Zhejiang, China: does ‘Same disease, same price’ matter by Meiteng Yu, Jing Liu, Tao Zhang

“…Abstract Background With the implementation of the hierarchical medical system (HMS) in China, Zhejiang Province introduced an innovative payment scheme called “payment method by disease types with point counting”. This scheme was initially adopted in Jinhua in July 2017, and was later integrated with the “same disease, same price” policy in Hangzhou in January 2020. …”
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Establishment of a rapid and quantitative method for detecting the range of infection exposure in preclinical dental education by Ayaka Ueda, Yuki Shinoda-Ito, Kazu Takeuchi-Hatanaka, Takashi Ito, Shintaro Ono, Kimito Hirai, Kazuhiro Omori, Tadashi Yamamoto, Shogo Takashiba

“…In vitro study, the correlation between adenosine triphosphate (ATP) levels and Lactobacillus colony numbers in yogurt was examined using the ATP fluorescent method, with colony counting on yogurt only and a mixture of yogurt and ultraviolet (UV)-sensitive hand lotion. …”
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Deciphering the Transcriptional Metabolic Profile of Adipose-Derived Stem Cells During Osteogenic Differentiation and Epigenetic Drug Treatment by Giulia Gerini, Alice Traversa, Fabrizio Cece, Matteo Cassandri, Paola Pontecorvi, Simona Camero, Giulia Nannini, Enrico Romano, Francesco Marampon, Mary Anna Venneri, Simona Ceccarelli, Antonio Angeloni, Amedeo Amedei, Cinzia Marchese, Francesca Megiorni

“…In this study, we employed the NanoString nCounter technology, an advanced multiplexed digital counting method of RNA molecules, to comprehensively characterize differentially expressed transcripts involved in metabolic pathways at distinct time points in osteogenically differentiating ASCs treated with or without the pan-DNMT inhibitor RG108. …”
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Mollusk Diversity in Percut Sei Tuan Silvofishery Ponds, North Sumatra, Indonesia by Ipanna Enggar Susetya, Mohammad Basyuni, Muhammad Rakezza Rifzy Rifzy, Rizka Amelia, Yuntha Bimantara, Rusdi Leidonald, Amanatul Fadhilah

“…Mollusk sampling was carried out on sub-plots measuring 1 x 1 m² in the mangrove vegetation transect, and mangrove density was measured by counting the number of trees directly on all of the mangrove species at two silvofishery ponds. …”
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The use of a benign fast-growing cyanobacterial species to control microcystin synthesis from Microcystis aeruginosa by Hakyung Lee, Vincent Xu, Jinjin Diao, Runyu Zhao, Moshan Chen, Tae Seok Moon, Haijun Liu, Kimberly M. Parker, Young-Shin Jun, Yinjie J. Tang

“…This study investigated the combined effects of nutrients and competition among cyanobacterial subpopulations on the synthesis of microcystin-LR.MethodsUnder varying nitrogen and phosphorus concentrations, cyanobacterial coculture, and the presence of algicidal DCMU, the growth was monitored by optical density analysis or microscopic counting, and the microcystin production was analyzed using high-performance liquid chromatography-UV. …”
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Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbits by Yang Chen, Tingting Lu, Yingying Dai, Yu Xue, Bohao Zhao, Xinsheng Wu

“…The impact of miR-222-3p on melanin synthesis was evaluated using the NaOH method, cell counting kit-8, and annexin V-fluorescein isothiocyanate/propidium iodide assays. …”
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SRT3025-loaded cell membrane hybrid liposomes (3025@ML) enhanced anti-tumor activity of Oxaliplatin via inhibiting pyruvate kinase M2 and fatty acid synthase by Xiaobin Wang, Shulin Li, Zichen Li, Zhuona Lin, Zhifeng Wang

“…Bladder cancer cell lines T24 and 5637 were cultured in McCoy’s 5 A and RPMI 1640 medium, respectively. The Cell Counting Kit-8 assay was used to determine the cell viability by treating cells with a medium containing either the vehicle solution (control), the cell membrane hybrid liposomes (ML), 3025@ML, or compound 3 K. …”
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Intermittent auscultation fetal monitoring during labour: A systematic scoping review to identify methods, effects, and accuracy. by Ellen Blix, Robyn Maude, Elisabeth Hals, Sezer Kisa, Elisabeth Karlsen, Ellen Aagaard Nohr, Ank de Jonge, Helena Lindgren, Soo Downe, Liv Merete Reinar, Maralyn Foureur, Aase Serine Devold Pay, Anne Kaasen

“…<h4>Background</h4>Intermittent auscultation (IA) is the technique of listening to and counting the fetal heart rate (FHR) for short periods during active labour and continuous cardiotocography (CTC) implies FHR monitoring for longer periods. …”
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High Concentration of Glucose Increases Reactive Oxygen Species Generation and Apoptosis Induced by Endoplasmic Reticulum Stress Pathway in Rabbit Corneal Epithelial Cells by Jiajun Lu, Minjie Sheng, Panpan Yao, Chaochao Ran, Hao Liu, Li Chen, Rui Liu, Bing Li

“…The expressions of PERK, p-PERK, Akt, p-Akt, and CHOP were determined by western blot, and the cell viability was measured by Cell Counting Kit-8 (CCK-8). Flow cytometry was used to detect the early apoptosis rate. …”
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The impact of sports experience on manual dexterity performances in school-age children by Antonino Patti, Domenico Savio Salvatore Vicari, Valerio Giustino, Flavia Figlioli, Genny Margherita Seidita, Alessandra Calogera Montalto Monella, Aurora Oddo, Antonio Paoli, Patrik Drid, Ewan Thomas, Antonino Bianco

“…The between-group variance in the finger-tapping test (GPT-FTT) and the counting test (GPT-CT) showed significant differences between groups (<0.001). …”
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All extern? Exploring the Impact of Social Media and Physical Activity on Adolescents’ Body Image and Interoception by Moritz Peter Engel, Mirko Schmidt

“…Interoceptive accuracy (Heartbeat Counting Task) and state positive body image (State Body Appreciation Scale, Body Functionality Scale) will be measured before and immediately after the experimental manipulations, Measurements will take place at a one-week interval. …”
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Activation of Delta-Opioid Receptor Protects ARPE19 Cells against Oxygen-Glucose Deprivation/Reoxygenation-Induced Necroptosis and Apoptosis by Inhibiting the Release of TNF-α by Runjie Guo, Ping Chen, Tiantian Fu, Ren Zhang, Yuan Zhu, Nange Jin, Hong Xu, Yong Xia, Xuesong Tian

“…Cell viability was quantified using the Cell Counting Kit-8 (CCK-8) assay. Morphological changes were observed by inverted microscopy. …”
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Vision Improvement after Osimertinib Treatment in Paraneoplastic Optic Neuropathy Associated with Lung Adenocarcinoma by Masaomi Kubota, Nobumasa Tamura, Takaaki Hayashi, Euido Nishijima, Haruhiko Yanagisawa, Akira Kojima, Tadashi Nakano

“…Her final BCVA was 0.01 in the RE, counting fingers 10 cm in the LE. She died at the age of 79 years. …”
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Nourishing change in Houston, Texas: exploring grocery shopping behaviours and fruit and vegetable consumption among low-income families in the Brighter Bites program by Victoria Kwentua, Allison Marshall, Ru-Jye Chuang, Jessica Chen, Christine Markham, Mallika Mathur, Mike Pomeroy, Megan Hall, Shreela Sharma

“…Children sometimes bring their own grocery lists, select their produce or help by counting produce; (2) the importance of balancing quality and affordability of fruits and vegetables purchased – both when selecting stores and choosing produce; (3) exposure to new varieties and higher quality of fruits and vegetables through Brighter Bites programming – parents/caregivers reported purchasing new fruits and vegetables as a result of participating in Brighter Bites. …”
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AxiWorm: a new tool using YOLOv5 to test antiparasitic drugs against Trichinella spiralis by Javier Sánchez-Montejo, Miguel Marín, María Alejandra Villamizar-Monsalve, María del Carmen Vieira, Belén Vicente, Rafael Peláez, Julio López-Abán, Antonio Muro

“…YOLOv5 is an image recognition tool easily trained to count muscular first stage larvae (L1) and recognize morphological differences. …”
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Danggui-Shaoyao-San Can Ameliorate Alzheimer&rsquo;s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology by Zhang KX, Zhang JW, Jiang YH, Wang YR, Liu ZL, Ding PL, Wang XY, Cui WQ, Xu XQ, Wang YH

“…Flow cytometry and Cell Counting Kit-8 (CCK-8) reveal the effect of DSS serum on HT-22 proliferation and apoptosis.Results: A total of 57 metabolic components were screened in DSS serum. …”
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Changes Related to Age in Natural and Acquired Systemic Self-IgG Responses in Malaria by Romuald Dassé, Didier Lefranc, Sylvain Dubucquoi, Patricia Dussart, Virginie Dutoit-Lefevre, Boualem Sendid, François Sombo Mambo, Patrick Vermersch, Lionel Prin

“…Using linear discriminant and principal component analysis, immune footprints were compared by counting the number, the presence or absence of reactive bands. …”
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GPR137-RAB8A activation promotes ovarian cancer development via the Hedgehog pathway by Chao Tang, Lin Li, Chongying Zhu, Qiang Xu, Zihao An, Shouying Xu, Chao Lin

“…The biological effects of GPR137-RAB8A-Hedgehog(HH) were investigated using in vitro and in vivo models and methods including qRT-PCR, RNA stability assay, RNA immunoprecipitation assay, GLI-luciferase reporter assay, nucleo-cytoplasmic separation assay, membrane-cytoplasmic separation assay, western blot, co-immunoprecipitation, immunofluorescence staining, cell counting kit-8 assay, wound healing assay, matrigel invasion assay, colony formation assay, xenografts assay, in situ transplantation tumor model of ovarian cancer in nude mice, and immunohistochemistry staining. …”
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多巴胺受体4对肝癌细胞增殖转移的影响及预后 by 严俨, 张红英, 潘家浩, 王冬音, 周晓爽, 元云飞, 曾维安, 陈东泰

“…【方法】通过免疫组化以及实时定量PCR（qPCR）技术检测肝    癌以及癌旁组织 DRD4 蛋白和 mRNA 表达情况，并分析 DRD4 表达的预后意义；采用 Cell Counting Kit 8（CCK8） 和 Transwell 实验检测 PD-168077 以及 L-745870 对肝癌细胞增殖、迁移及侵袭的作用，蛋白免疫印迹（Western blot）技术检测信号通路蛋白，并使用通路抑制剂验证。…”
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