Clinical Features of Combined Central Retinal Artery and Vein Occlusion by Hao Wang, Yongye Chang, Fen Zhang, Rong Yang, Suxia Yan, Jieying Dong, Minglian Zhang, Shaomin Peng

“…In addition, 45.5% (15/33) of the eyes had BCVA of finger counting (FC) or below, whereas 12.1% (4/33) had BCVA of 20/60 or above. …”
Get full text

RETRACTED ARTICLE: NR3C2 affects the proliferation and invasiveness of colon cancer cells through the Wnt/β-Catenin signaling pathway by Ke Nie, Zhong-Jiang He, Ling-Jun Kong

“…Furthermore, colon cancer cell models overexpressing NR3C2 and with knockdown NR3C2 were constructed by lentiviral vector transfection. Cell Counting Kit-8 assay, transplantation of tumors in nude mice and transwell assays were used to examine the proliferation, migration and invasion of colon cancer cells. …”
Get full text

A Method for Quantifying Mung Bean Field Planting Layouts Using UAV Images and an Improved YOLOv8-obb Model by Kun Yang, Xiaohua Sun, Ruofan Li, Zhenxue He, Xinxin Wang, Chao Wang, Bin Wang, Fushun Wang, Hongquan Liu

“…These findings can be utilized for rapid and large-scale assessments of mung bean seedling growth and development, providing theoretical and technical support for seedling counting and planting layouts in hole-seeded crops.…”
Get full text

MicroRNA-21-5p Reduces Hypoxia/Reoxygenation-Induced Neuronal Cell Damage through Negative Regulation of CPEB3 by Bin Wang, Ping Yu, Wei Lin, Zhaohui Zhai

“…The hypoxia/reoxygenation (H/R) model was established in mouse neuronal cells HT22. Cell Counting Kit-8 (CCK-8) and qRT-PCR were used to determine the effects of H/R treatment on cell viability and miR-21-5p expression. …”
Get full text

Epigallocatechin-3-gallate inhibits the collagen accumulation of oral submucous fibrosis induced by arecoline by Ge Gao, Ge Gao, Caipeng Lin, Caipeng Lin, Ruibo Li, Xi Xie, Hai-Bin Luo, Hai-Bin Luo

“…Optimal ARE concentrations were established using the Cell Counting Kit-8. The impact of ARE on extracellular matrix (ECM)-related protein expression was assessed through RT-qPCR and Western blot techniques. …”
Get full text

Mechanism of Action of Decitabine in the Treatment of Acute Myeloid Leukemia by Regulating LINC00599 by Fan Du, Ting Jin, Li Wang

“…Cell proliferation in each group was detected using the cell counting kit 8. For each group, apoptosis and reactive oxygen species (ROS) levels were detected using flow cytometry. …”
Get full text

Anti-colorectal cancer effect of total minor ginsenosides produced by lactobacilli transformation of major ginsenosides by inducing apoptosis and regulating gut microbiota by Yunjiao Shen, Yunjiao Shen, Yansong Gao, Ge Yang, Zijian Zhao, Yujuan Zhao, Lei Gao, Shengyu Li

“…Total minor ginsenosides (TMG) were obtained through the fermentation of major ginsenosides with Lactiplantibacillus plantarum, and potential anticancer effects of TMGs on the mouse colon cancer cell line CT26.WT, in vitro and in vivo, were investigated.Materials and MethodsWe employed the Cell Counting Kit-8 (CCK-8), TdT-mediated dUTP nick end labeling (TUNEL), and Western blot analysis in vitro to explore the anti-proliferative and pro-apoptotic functions of TMG in CT26.WT cells. …”
Get full text

Curcumin nanocrystals ameliorate ferroptosis of diabetic nephropathy through glutathione peroxidase 4 by Mengjiao Xue, Mengjiao Xue, Yiwei Tian, Hua Zhang, Hua Zhang, Shijie Dai, Yangsheng Wu, Juan Jin, Juan Jin, Jian Chen

“…Then, cell viability was evaluated in HK-2 cells treated with varying concentrations of Cur-NCs (0.23, 0.47, 0.94, 1.87, 3.75, 7.5, 15, and 30 μg/mL) using Cell Counting Kit-8 (CCK-8). Furthermore, in vivo experiments were carried out to investigate the roles of Cur-NCs in STZ-induced DN rats.ResultsThe results showed that HG treatment greatly enhanced the levels of LDH, MDA, Iron, lipid ROS, apoptosis, NCOA4, TFR-1, while decreasing the expression of GSH, GPX4, SLC7A11, and FTH-1. …”
Get full text

An AI-Based Digital Scanner for <i>Varroa destructor</i> Detection in Beekeeping by Daniela Scutaru, Simone Bergonzoli, Corrado Costa, Simona Violino, Cecilia Costa, Sergio Albertazzi, Vittorio Capano, Marko M. Kostić, Antonio Scarfone

“…Given its repeatability and reliability, the device can be considered a valid tool for beekeepers and scientists, offering the opportunity to monitor many beehives in a short time, unlike visual counting, which is done on a sample basis.…”
Get full text

Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis by Juliana Carvalho de Quaglia e Silva, Amanda Manoel Della Coletta, Taiane Priscila Gardizani, Graziela Gorete Romagnoli, Ramon Kaneno, Luciane Alarcão Dias-Melicio

“…Therefore, analysis of Dectin-1 receptor expression was done by flow cytometry whereas the effector mechanisms were evaluated by fungal recovery by colony-forming unit (CFU) counting and hydrogen peroxide (H2O2) production. Our results showed that, after treatment with IFN-γ, TNF-α, and GM-CSF and challenge with Pb265, cells, especially monocytes, demonstrated an increase in Dectin-1 expression. …”
Get full text

ELAVL1‐dependent SOAT2 exacerbated the pancreatitis‐like cellular injury of AR42J cells induced by hyperstimulation with caerulein by Yu‐Jing Sun, Hua‐Ying Chen, Xiao‐Qin Lai

“…Cell viability was determined using a cell counting kit‐8 (CCK‐8) assay, while cell proliferation was analyzed through a 5‐Ethynyl‐2′‐deoxyuridine assay. …”
Get full text

Using a Simplified Quantum Counter to Implement Quantum Circuits Based on Grover’s Algorithm to Tackle the Exact Cover Problem by Jehn-Ruey Jiang, Yu-Jie Wang

“…For the whole quantum circuit, the simplified quantum counter saves <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mrow><mo>(</mo><mn>4</mn><mi>m</mi><mi>b</mi><mo>−</mo><mn>4</mn><mi>m</mi><mo>)</mo></mrow><mrow><mo>⌊</mo><mi>π</mi><mo>/</mo><mn>4</mn><msqrt><mrow><mi>N</mi><mo>/</mo><mi>M</mi></mrow></msqrt><mo>⌋</mo></mrow></mrow></semantics></math></inline-formula> quantum gates and reduces the quantum circuit depth by <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mrow><mo>(</mo><mn>2</mn><mi>m</mi><mi>b</mi><mo>)</mo></mrow><mrow><mo>⌊</mo><mi>π</mi><mo>/</mo><mn>4</mn><msqrt><mrow><mi>N</mi><mo>/</mo><mi>M</mi></mrow></msqrt><mo>⌋</mo></mrow></mrow></semantics></math></inline-formula> compared to Heidari et al.’s design, where <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>b</mi><mo>=</mo><mo>⌊</mo><mo form="prefix">log</mo><mi>n</mi><mo>⌋</mo><mo>+</mo><mn>1</mn></mrow></semantics></math></inline-formula> is the number of counting qubits used in a counter. Experimental results obtained using IBM Qiskit packages confirm the effectiveness of our quantum circuits.…”
Get full text

A Lightweight YOLO Model for Rice Panicle Detection in Fields Based on UAV Aerial Images by Zixuan Song, Songtao Ban, Dong Hu, Mengyuan Xu, Tao Yuan, Xiuguo Zheng, Huifeng Sun, Sheng Zhou, Minglu Tian, Linyi Li

“…Accurate counting of the number of rice panicles per unit area is essential for rice yield estimation. …”
Get full text

Inhibition of complement system-related gene ITGB2 attenuates epithelial–mesenchymal transition and inflammation in diabetic nephropathy by Jun Peng, Wenqi Zhao, Lu Zhou, Kun Ding

“…Then the cell viability, inflammatory factors, and the expression of epithelial–mesenchymal transition (EMT) and fibrosis markers were determined by using Cell Counting Kit-8 (CCK-8) assay, enzyme linked immunosorbent assay (ELISA), western blotting assays, respectively. …”
Get full text

Effects of environmental bisphenol S exposure on male rat reproductive health and gut-blood-testicular axis integrity by Biran Zhu, Zequan Zhang, Ying Xie, Min Huang, Yu Chen, Yong Yang, Xiongjie Shi, Jian Han, Lihua Yang, Min Zhao

“…These observations were made by counting with a hemocytometer under the optical microscope. 16S rRNA and untargeted metabolomic elucidated potential impacts on the gut-blood-testicular axis: BPS impaired the physical barrier, evoked inflammation, and resulted in dysbiosis of the gut microbiota. …”
Get full text

Establishing a Surgical Procedure for Rhesus Epiretinal Scaffold Implantation with HiPSC-Derived Retinal Progenitors by Ziming Luo, Kang Li, Kaijing Li, Bikun Xian, Ying Liu, Sijing Yang, Chaochao Xu, Zhigang Fan, Shoutao Lu, Haijun Zhang, Jian Ge

“…Then, the cell survival rate was evaluated by Cell Counting Kit-8 (CCK-8). Second, three methods of scaffold fixation, including adhesion after gas-liquid exchange (n=1), tamponade by hydrogel (n=1), and fixation by retinal tacks (n=4), were performed in rhesus monkeys. …”
Get full text

Inhibition of triple-negative breast cancer growth via delphinidin-mediated suppression of the JAK2/STAT3/PD-L1 pathway by Xiaoping Yu, Xiaolong Song, Jiali Yan, Ziting Xiong, Lujie Zheng, Yan Luo, Fengcheng Deng, Yanfeng Zhu

“…Design: We utilized cell counting kit-8 (CCK-8) assays, colony formation assays, and scratch assays to evaluate the influence of Dp on the proliferation and migration of TNBC cells. …”
Get full text

CST2 promotes cell proliferation and regulates cell cycle by activating Wnt-β-catenin signalling pathway in serous ovarian cancer by Xiaohua Wang, Sufen Zhao, Yanwei Guo, Chunhui Wang, Shuyu Han, Xingcha Wang

“…Cell cycle characteristics and proliferation were assessed using a colony formation assay, flow cytometry, and a cell counting kit-8 assay. Western blots and quantitative reverse transcription PCR analyses were employed to examine CST2 expressions and related genes involved in the cell cycle and the Wnt-β-catenin signalling pathway.Results Our findings revealed significant upregulation of CST2 in SOC, and elevated CST2 expression was correlated with advanced clinicopathological characteristics and unfavourable prognoses. …”
Get full text

A Survey of Camouflaged Object Detection and Beyond by Fengyang Xiao, Sujie Hu, Yuqi Shen, Chengyu Fang, Jinfa Huang, Longxiang Tang, Ziyun Yang, Xiu Li, Chunming He

“…Beyond object-level detection, we also summarize extended methods for instance-level tasks, including camouflaged instance segmentation, counting, and ranking. Additionally, we provide an overview of commonly used benchmarks and evaluation metrics in COD tasks, conducting a comprehensive evaluation of deep learning-based techniques in both image and video domains, considering both qualitative and quantitative performance. …”
Get full text

Green analysis of illicit drugs on banknote dust by Teresa Cecchi, Arianna Giuliani, Carlo Catini

“…Purpose: This study aims to develop and validate the first analytical method to assess the presence of common illicit drugs on banknote dust from counting machines via green preanalytical and analytical approaches. …”
Get full text