Alleviating the IL-1β-stimulated extracellular matrix degradation in osteoarthritis, and chondrocyte inflammation by Morinda officinalis polysaccharide via the SIRT6/NF-κB pathway... by Dongfang Zhao, Shuqin Xing, Jiao Qi, Zhiqiang Wei, Jianghai Huang, Jigao Sun, Xinzhu Wen, Yafei Wang

“…To investigate, CCK-8 (cell counting kit-8) assays were performed to evaluate MOP’s impact on the viability of human chondrocytes (C28/I2 cells). …”
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Simultaneous central retinal vein and cilioretinal artery occlusion in a young female patient with progestin-only oral contraceptive use: a case report by Sardar Noman Qayyum, Risha Naeem, Faraz Azhar, Gulmeena Aziz Khan, Tehreem Zubair, Samim Noori

“…Visual acuity in the affected eye was limited to counting fingers at a distance of 0.5 feet, with a best-corrected visual acuity of 6/6 in the right eye. …”
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The Combined Use of Platelet-Rich Plasma Clot Releasate and Allogeneic Human Umbilical Cord Mesenchymal Stem Cells Rescue Glucocorticoid-Induced Osteonecrosis of the Femoral Head by Yanxue Wang, Shuo Luan, Ze Yuan, Shaoling Wang, Shengnuo Fan, Chao Ma, Shaoling Wu

“…To evaluate the combinatorial therapeutic effects of platelet-rich plasma clot releasate (PRCR) and umbilical cord mesenchymal stem cells (UC-MSCs) on glucocorticoid-induced ONFH, a dexamethasone (DEX)-treated cell model and a high-dose methylprednisolone (MPS)-treated rat model were established. Cell counting kit-8 (CCK-8) assay was performed in vitro to determine the optimum dosage of PRCR for UC-MSC viability. …”
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Preparation and In Vitro Evaluation of Alginate Microparticles Containing Amphotericin B for the Treatment of Candida Infections by Merlis P. Alvarez-Berrios, Lisa M. Aponte-Reyes, Lourdes Diaz-Figueroa, Juan Vivero-Escoto, Alexis Johnston, David Sanchez-Rodriguez

“…To prove this hypothesis, the ability of the microplatform to disrupt the cellular membrane potential was tested and its antifungal effectiveness toward Candida albicans cells was evaluated using the cell counting and plate count methods. Moreover, the toxicity of the microplatform in human lung cells was evaluated using CellTiter 96® AQueous cell viability assay and qualitative diffusion analysis of acridine orange. …”
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Thermal properties and non-bonded interactions in human PMP2 variants: A molecular dynamics study by Prabin Aryal, Jhulan Powrel

“…Using the NAMD software for molecular dynamics simulations, various analyses were performed, including RMSD estimation, counting number of hydrogen bond formation, salt bridge occupancy assessment, estimation of vdW and electrostatic interaction energies in the system, and estimation of thermal diffusivity of the proteins as well as specific heat capacity (Cv) of the system. …”
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Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis by Yang Sun, Sanmao Su, Mengjun Li, Ang Deng

“…The protein FGF9 was tested by western blotting. Cell counting kit-8 (CCK8), plate cloning assay, and flow cytometry were conducted to evaluate cell proliferation and apoptosis. …”
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Therapeutic Effects of GDF6-Overexpressing Mesenchymal Stem Cells through Upregulation of the GDF15/SIRT1 Axis in Age-Related Hearing Loss by Jiali Liu, Haisen Peng, Yuehui Liu, Chunhua Li, Wen Xie

“…MSC proliferation was evaluated with the Cell Counting Kit-8 assay. …”
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Regulatory factor X‐5/SCL/TAL1 interruption site axis promotes aerobic glycolysis and hepatocellular carcinoma cell stemness by Zhi‐Zhong Zhang, Zi‐Ming Wang, Hao‐Wen Zhang, Yan‐Xin Gong, Hao‐Ran Sun, Wei Zhang

“…The viability of the cells was assessed by the Cell Counting Kit‐8 assay. The sphere formation assay was used to evaluate the sphere‐forming capacity. …”
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Prevalence and density of Elaphostrongylus rangiferi larvae in faecal samples of semi-domestic reindeer (Rangifer tarandus tarandus) in Norway 2013-16 by Terje Domaas Josefsen, Torill Mørk, Ingebjørg Helena Nymo, Javier Sanchez Romano, Morten Tryland

“…The density of larvae was measured by counting and calculating number of larvae per gram faeces (LPG) in positive samples. …”
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Effect of Activated Platelet-Rich Plasma on Chondrogenic Differentiation of Rabbit Bone Marrow-Derived Mesenchymal Stem Cells by Zhen Wang, Zheng Wang, Bin Zhang, Qinghua Zhao, Yubao Liu, Wei Qi

“…Passage 3 BMSCs were cultured in high-glucose Dulbecco’s modified Eagle’s medium (HG-DMEM) with the four different compositions for consecutive 7 days, including 10% fetal bovine serum, 5% PRP, 10% PRP, and 15% PRP. Cell counting assays were performed to evaluate the cell proliferation of BMSCs. …”
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SIRT5‐mediated HOXA5 desuccinylation inhibits ferroptosis to alleviate sepsis induced‐lung injury by Lei Wang, Heng Fan, Min Sun, Ji‐Hui Ye

“…Cell viability, ferrous iron (Fe2+) level, and cellular lipid reactive oxygen species (ROS) were determined with Cell Counting Kit‐8 assay, iron assay kit, and BODIPY™ 665/676 molecular probe, respectively. …”
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An In Vitro Model of Diabetic Retinal Vascular Endothelial Dysfunction and Neuroretinal Degeneration by Qiyun Wang, Xinyuan Zhang, Kaiyue Wang, Ling Zhu, Bingjie Qiu, Xiaosi Chen, Xiao Lin, Yao Nie

“…Cell proliferation and viability were evaluated by the Cell Counting Kit-8. Cell migration was measured using a transwell migration assay. …”
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Exploration on factors influencing HLA-C molecular expression level by flow cytometry by LI Yunan, JIANG Renhui, CAI Siqi, LIU Jie, DENG Zhihui

“…[Methods] A total of 12 hematopoietic stem cell suspension samples from peripheral hematopoietic stem cell volunteer donors were randomly collected after CD34+ cell counting detection. The influence of detecting different number of nucleated cell (500 000, 50 000 and 5 000), sequential order of red blood cell lysis and antibody incubation, and the HLA-C antibody with varied remaining time from the expiration date on the detection results of HLA-C expression by flow cytometry were investigated, respectively. …”
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Assessment of Microbiological Indoor Air Quality in Cattle Breeding Farms by Justyna Szulc, Małgorzata Okrasa, Katarzyna Dybka-Stępień, Michael Sulyok, Adriana Nowak, Anna Otlewska, Bogumiła Szponar, Katarzyna Majchrzycka

“…Abstract This study, which was conducted on a farm in central Poland from February till April 2019, aimed to evaluate microbiological threats on cattle breeding premises by counting and identifying the microorganisms (via culturing and high-throughput sequencing), assessing the endotoxin concentrations (via gas chromatography-mass spectrometry [GC-MS]) and analysing the secondary metabolites (including mycotoxins) in the air and sedimented dust (via liquid chromatography-tandem mass spectrometry [LC-MS/MS]) in six barns. …”
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Ghrelin通过JNK信号通路抑制LPS诱导的肺泡巨噬细胞凋亡

“…【目的】 观察体外条件下加入外源性ghrelin对内毒素(LPS)诱导大鼠肺泡巨噬细胞NR8383凋亡以及吞噬能力的影响,并探讨JNK(c-Jun N-terminal kinase)信号通路在其中所起的作用? 【方法】 用CCK-8(Cell Counting Kit-8)法检测加入LPS或者ghrelin与LPS共孵育后NR8383的细胞毒性;流式细胞技术?…”
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Prognostic significance of CDK1 expression in diffuse large B-Cell lymphoma by Qiuni Chen, Lei Xu, Chuanyang Lu, Yujie Xue, Xue Gong, Yuye Shi, Chunling Wang, Liang Yu

“…Additionally, we investigated the effect of CDK1 inhibition on DLBCL cell growth and death using the Cell Counting Kit-8 and flow cytometry. Results In the control group, CDK1 expression was predominantly observed in the hematopoietic and reproductive systems. …”
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CircBAZ1B stimulates myocardial ischemia/reperfusion injury (MI/RI) by modulating miR-1252-5p/ATF3-mediated ferroptosis by Ruili Wei, Tianxiao Yang, Weihong Li, Xiqian Wang

“…Cell viability, apoptosis, lipid reactive oxygen species (ROS) levels and iron content were determined via Cell Counting Kit-8 (CCK-8) and flow cytometric assays. …”
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The antibiofilm effect and mechanism of silver nanowire-modified glass ionomer cement against multi-species oral biofilm by Tiantian Guo, Dai Wang, Sherry Shiqian Gao

“…Colour stability and fluoride release of GIC specimens were assessed by VITA Easyshade® V and ion chromatography. Cell counting kit-8 (CCK-8) was used to study cytotoxicity. …”
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Thermal properties and non-bonded interactions in human PMP2 variants: A molecular dynamics study by Prabin Aryal, Jhulan Powrel

“…Using the NAMD software for molecular dynamics simulations, various analyses were performed, including RMSD estimation, counting number of hydrogen bond formation, salt bridge occupancy assessment, estimation of vdW and electrostatic interaction energies in the system, and estimation of thermal diffusivity of the proteins as well as specific heat capacity (Cv) of the system. …”
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Research on machine vision online monitoring system for egg production and quality in cage environment by Zhenlong Wu, Hengyuan Zhang, Cheng Fang

“…To diverse operational needs, two distinct post-processing algorithms were developed: one for counting eggs and detecting abnormalities during robotic patrols, and another for assessing egg quality through fixed video streams, which measured crucial parameters such as egg dimensions and shape indexes. …”
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