The use of a benign fast-growing cyanobacterial species to control microcystin synthesis from Microcystis aeruginosa by Hakyung Lee, Vincent Xu, Jinjin Diao, Runyu Zhao, Moshan Chen, Tae Seok Moon, Haijun Liu, Kimberly M. Parker, Young-Shin Jun, Yinjie J. Tang

“…This study investigated the combined effects of nutrients and competition among cyanobacterial subpopulations on the synthesis of microcystin-LR.MethodsUnder varying nitrogen and phosphorus concentrations, cyanobacterial coculture, and the presence of algicidal DCMU, the growth was monitored by optical density analysis or microscopic counting, and the microcystin production was analyzed using high-performance liquid chromatography-UV. …”
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Iguratimod Inhibits the Aggressiveness of Rheumatoid Fibroblast-Like Synoviocytes by Jin Lin, Ye Yu, Xuanwei Wang, Yini Ke, Chuanyin Sun, Lihuan Yue, Guanhua Xu, Bei Xu, Liqin Xu, Heng Cao, Danyi Xu, Nancy Olsen, Weiqian Chen

“…The proliferation of RA-FLSs was assessed by 5-ethynyl-2′-deoxyuridine test and Cell Counting Kit-8. Migration and invasion were determined by the wound test and a transwell assay. …”
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Therapeutic Effects of GDF6-Overexpressing Mesenchymal Stem Cells through Upregulation of the GDF15/SIRT1 Axis in Age-Related Hearing Loss by Jiali Liu, Haisen Peng, Yuehui Liu, Chunhua Li, Wen Xie

“…MSC proliferation was evaluated with the Cell Counting Kit-8 assay. …”
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Comparison of Cycle Outcomes among Women with Endometrioma or Non-Functional Cysts Versus Control by Enis Ozkaya, Ebru Cogendez, Elif Tozkir, Saadet Unsal, Ahmet Yavuz, Gulden Tunali, Ozgur Uzun, Evrim Bostancı Ergen, Semra Kayatas Eser

“…Baseline total antral follicle counts were similar among groups (p>0.05). There were significant differences among groups in terms of mean starting and total gonadotropin dose, peak estradiol level, total oocyte number, and mature oocyte numbers (p<0.05). …”
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Anti-colorectal cancer effect of total minor ginsenosides produced by lactobacilli transformation of major ginsenosides by inducing apoptosis and regulating gut microbiota by Yunjiao Shen, Yunjiao Shen, Yansong Gao, Ge Yang, Zijian Zhao, Yujuan Zhao, Lei Gao, Shengyu Li

“…Total minor ginsenosides (TMG) were obtained through the fermentation of major ginsenosides with Lactiplantibacillus plantarum, and potential anticancer effects of TMGs on the mouse colon cancer cell line CT26.WT, in vitro and in vivo, were investigated.Materials and MethodsWe employed the Cell Counting Kit-8 (CCK-8), TdT-mediated dUTP nick end labeling (TUNEL), and Western blot analysis in vitro to explore the anti-proliferative and pro-apoptotic functions of TMG in CT26.WT cells. …”
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Revisiting Fat Graft Harvesting and Processing Technique to Optimize Its Regenerative Potential by Karina Karina, MD, PhD, Johannes A. Biben, MD, Krista Ekaputri, MD, Grady Krisandi, BMed, Imam Rosadi, MSc, Siti Sobariah, BSc, Irsyah Afini, MBiomed, Tias Widyastuti, BSc, Alfida Zakiyah, BSc, Difky Ernanda, BSc

“…The mean stromal cell counts were 0.937 × 109 ± 0.346 × 109/mL for cannula A and 0.734 × 109 ± 0.266 × 109/mL for cannula B (P = 0.684), with viabilities of 98.79% and 98.22% (P = 0.631), respectively. …”
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Wenxin Granules Influence the TGFβ-P38/JNK MAPK Signaling Pathway and Attenuate the Collagen Deposition in the Left Ventricle of Myocardial Infarction Rats by Ya Huang, Aiming Wu, Lixia Lou, Dongmei Zhang, Bo Nie, Yizhou Zhao, Keke Liu, Mingjing Zhao, Hongcai Shang

“…The changes of cardiac hemodynamics were detected by the catheter method, morphological changes were observed by HE staining, and myocardial tissue collagen volume was counted by Immunohistochemistry combined with Masson staining, and the expression of inflammatory TGFβ-p38/JNK MAPK signal pathway markers was detected by Western blot. …”
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The hypoxia adaptation strategy of the big-belly seahorses (Hippocampus abdominalis) with tuft gills by Long-Wu Jia, Le-Le Zhang, Hong-Wei Yang, Chun-Yu Hou, Lin Shi, Yu-Ping Hou, Guo-Dong Zheng, Xiao-Lei Su, Kai Wang

“…After 4- or 7-days of hypoxia treatment, the red blood cell counts, SOD, CAT, GSH, and T-AOC of BBS were significantly (P < 0.05) higher than those in the normoxia group. …”
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Interpretation and classification of FBN1 variants associated with Marfan syndrome: consensus recommendations from the Clinical Genome Resource’s FBN1 variant curation expert panel... by A. Drackley, C. Somerville, P. Arnaud, L. M. Baudhuin, N. Hanna, M. L. Kluge, K. Kotzer, C. Boileau, L. Bronicki, B. Callewaert, A. Cecchi, H. Dietz, D. Guo, S. Harris, O. Jarinova, M. Lindsay, L. Little, B. Loeys, G. MacCarrick, J. Meester, D. Milewicz, T. Morisaki, H. Morisaki, D. Murdock, M. Renard, J. Richer, L. Robert, M. Ouzounian, L. Van Laer, J. De Backer, L. Muiño-Mosquera

“…Some of these specifications include refining the minor allele frequency thresholds, creating a FBN1-specific flowchart for PVS1, defining functional domains of the protein, developing a point-based system of counting probands and instances of de novo occurrences, recommending a points-based method of accounting for family segregation data, and clarifying the applicable functional assays that should be considered. …”
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Effect of UV-C Postharvest Disinfection on the Quality of Fresh-Cut 'Tommy Atkins' Mango by Alba Mery Garzón-García, Saúl Ruiz-Cruz, Saúl Dussán-Sarria, José Igor Hleap-Zapata, Enrique Márquez-Ríos, Carmen Lizette Del-Toro-Sánchez, José Agustín Tapia-Hernández, Dalila Fernanda Canizales-Rodríguez, Víctor Manuel Ocaño-Higuera

“…Physicochemical analysis (titratable acidity, pH, total soluble solid content, and firmness), superficial color evaluation, determinations of microbial counts, contents of total carotenoids, phenolics and flavonoids, and antioxidant capacity assays were performed. …”
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An AI-Based Digital Scanner for <i>Varroa destructor</i> Detection in Beekeeping by Daniela Scutaru, Simone Bergonzoli, Corrado Costa, Simona Violino, Cecilia Costa, Sergio Albertazzi, Vittorio Capano, Marko M. Kostić, Antonio Scarfone

“…Given its repeatability and reliability, the device can be considered a valid tool for beekeepers and scientists, offering the opportunity to monitor many beehives in a short time, unlike visual counting, which is done on a sample basis.…”
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Unveiling the novel role of circadian rhythms in sepsis and septic shock: unexplored implications for chronotherapy by Stewart D. Ramsay, Stewart D. Ramsay, Stewart D. Ramsay, Marni A. Nenke, Marni A. Nenke, Emily J. Meyer, Emily J. Meyer, David J. Torpy, David J. Torpy, Richard L. Young, Richard L. Young, Richard L. Young, Richard L. Young

“…Examples of circadian disruptions during sepsis and septic shock include alteration or loss of hormonal rhythms controlling blood pressure and inflammation, white blood cell counts, and cytokine secretions. These changes to circadian rhythms hinder sepsis and septic shock recovery and also increase mortality. …”
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Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis by Juliana Carvalho de Quaglia e Silva, Amanda Manoel Della Coletta, Taiane Priscila Gardizani, Graziela Gorete Romagnoli, Ramon Kaneno, Luciane Alarcão Dias-Melicio

“…Therefore, analysis of Dectin-1 receptor expression was done by flow cytometry whereas the effector mechanisms were evaluated by fungal recovery by colony-forming unit (CFU) counting and hydrogen peroxide (H2O2) production. Our results showed that, after treatment with IFN-γ, TNF-α, and GM-CSF and challenge with Pb265, cells, especially monocytes, demonstrated an increase in Dectin-1 expression. …”
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Nourishing change in Houston, Texas: exploring grocery shopping behaviours and fruit and vegetable consumption among low-income families in the Brighter Bites program by Victoria Kwentua, Allison Marshall, Ru-Jye Chuang, Jessica Chen, Christine Markham, Mallika Mathur, Mike Pomeroy, Megan Hall, Shreela Sharma

“…Children sometimes bring their own grocery lists, select their produce or help by counting produce; (2) the importance of balancing quality and affordability of fruits and vegetables purchased – both when selecting stores and choosing produce; (3) exposure to new varieties and higher quality of fruits and vegetables through Brighter Bites programming – parents/caregivers reported purchasing new fruits and vegetables as a result of participating in Brighter Bites. …”
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Prognostic significance of CDK1 expression in diffuse large B-Cell lymphoma by Qiuni Chen, Lei Xu, Chuanyang Lu, Yujie Xue, Xue Gong, Yuye Shi, Chunling Wang, Liang Yu

“…Additionally, we investigated the effect of CDK1 inhibition on DLBCL cell growth and death using the Cell Counting Kit-8 and flow cytometry. Results In the control group, CDK1 expression was predominantly observed in the hematopoietic and reproductive systems. …”
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Clinical Presentation and Course of Pulmonary Involvement in Chronic Nonbacterial Osteomyelitis by Toni Hospach, Friederike Blankenburg, Anita Heinkele, Kristina Rücklová, Peter Müller‐Abt, Frank Weller‐Heinemann, Laura Buchtala, Jan Maier, Jasmin Kümmerle‐Deschner, Christiane Reiser, Ales Janda, Andreas Urban, Reiner Berendes, Andrea Skrabl‐Baumgartner, Jürgen Brunner, Ralf Trauzeddel, Johannes Peter Haas, Annette Holl‐Wieden, Henner Morbach, Anja Schnabel, Normi Brück, Gabriele Hahn, Wolfgang Emminger, Isabella Valent, Regine Borchers, Christian Klemann, Ilias Tsiflikas, Jens Klotsche, Kirsten Minden, Christian M. Hedrich, Thekla von Kalle

“…Forty‐two pulmonary lesions were counted with a median of two per patient (two to six). …”
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Telemedicine in home enteral nutrition: a structured survey exploring acceptability, preferences and experiences among patients by Ya Ma, Xuemei Li, Qian You, Wen Hu, Zhiyong Rao, Yanru Deng, Yiyao Zhang, Lei Shi

“…Methods The revisit station of HEN patients between 2018 and 2022 were counted. And epidemiological characteristics of HENST patients in 2022 were collected. …”
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Viable but nonculturable state in the zoonotic pathogen Bartonella henselae induced by low-grade fever temperature and antibiotic treatment by Yuze Gou, Yuze Gou, Dongxia Liu, Dongxia Liu, Yuxian Xin, Yuxian Xin, Ting Wang, Jiaxin Li, Yiwen Xi, Xiaoling Zheng, Tuanjie Che, Ying Zhang, Ying Zhang, Tingting Li, Jie Feng, Jie Feng

“…Viability was assessed using SYBR Green I/PI staining and propidium monoazide–quantitative polymerase chain reaction (PMA-qPCR), while culturability was determined through colony-forming unit (CFU) counting on blood agar plates. Resuscitation of VBNC cells was attempted using modified Schneider’s medium with 10% defibrillated sheep blood. …”
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A Lightweight YOLO Model for Rice Panicle Detection in Fields Based on UAV Aerial Images by Zixuan Song, Songtao Ban, Dong Hu, Mengyuan Xu, Tao Yuan, Xiuguo Zheng, Huifeng Sun, Sheng Zhou, Minglu Tian, Linyi Li

“…Accurate counting of the number of rice panicles per unit area is essential for rice yield estimation. …”
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The Upregulation of COX2 in Human Degenerated Nucleus Pulposus: The Association of Inflammation with Intervertebral Disc Degeneration by Chang Liu, Guoyan Liang, Zhantao Deng, Jing Tan, Qiujian Zheng, Feng-Juan Lyu

“…The total integrated COX2 optical density (IOD), number of COX2-positive cells, and total cell number of each image were counted and analyzed by Image-Pro Plus software. The IOD and number of COX2-positive cells were divided by the total cell number to obtain COX2 expression density (IOD/cell) and COX2 positivity (cell+/cell). …”
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