Driving effect of P16 methylation on telomerase reverse transcriptase-mediated immortalization and transformation of normal human fibroblasts by Xuehong Zhang, Paiyun Li, Ying Gan, Shengyan Xiang, Liankun Gu, Jing Zhou, Xiaorui Zhou, Peihuang Wu, Baozhen Zhang, Dajun Deng, Jing Ni

“…A zinc finger protein-based P16-specific DNA methyltransferase (P16-Dnmt) vector containing a “Tet-On” switch was used to induce extensive methylation of P16 CpG islands in normal human fibroblast CCD-18Co cells. …”
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Bearberry in the treatment of acute uncomplicated cystitis (BRUMI): protocol of a multicentre, randomised double-blind clinical trial by Péter Hegyi, Noémi Gede, Szilárd Váncsa, Barbara Tóth, András Jávorházy, Péter Nyirády, Boglárka Csupor-Löffler, Péter Birinyi, George Zhanel, Kurt Naber, Reinhard Länger, Nóra Vörhendi, Dezső Csupor

“…Patients will be randomly assigned to receive 3 g single dose of fosfomycin powder and two placebo tablets three times a day for 7 days or B a single dose of placebo powder and two tablets containing a dry extract of Uvae ursi folium. At least 504 patients (allocated as 1:1) will need to be enrolled to access non-inferiority with a non-inferiority limit of 14% for the primary endpoint.Improvement of symptoms of uncomplicated cystitis (based on the ACSS score) at day 7 is defined as the primary endpoint, whereas several secondary endpoints such as the number and ratio of patients with bacteriuria at day 7, frequency and severity of side effects; recurrence of urinary tract infection, concurrent use of other over the counter (OTC) medications and food supplements will be determined to elucidate more detailed differences between the groups. …”
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Biodegradation of Phenol at High Initial Concentration by <i>Rhodococcus opacus</i> 3D Strain: Biochemical and Genetic Aspects by Tatiana O. Anokhina, Tatiana Z. Esikova, Valentina N. Polivtseva, Nataliya E. Suzina, Inna P. Solyanikova

“…Phenolic compounds are an extensive group of natural and anthropogenic organic substances of the aromatic series containing one or more hydroxyl groups. The main sources of phenols entering the environment are waste from metallurgy and coke plants, enterprises of the leather, furniture, and pulp and paper industries, as well as wastewater from the production of phenol–formaldehyde resins, adhesives, plastics, and pesticides. …”
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Sustainable geopolymer adsorbents utilizing silica fume as a partial replacement for metakaolin in the removal of copper ion from synthesized copper solution by Pilomeena Arokiasamy, Mohd Mustafa Al Bakri Abdullah, Eva Arifi, Noorina Hidayu Jamil, Md Azree Othuman Mydin, Shayfull Zamree Abd Rahim, Andrei Victor Sandu, Shafiq Ishak

“…The generation of N-A-S-H gel with the 75 % replacement level of S1 producing more reactive Si and Al binding sites for Cu2+ adsorption. In addition, S1 contains exchangeable cations such as Ca2+, Mg2+ and Na+ which further promote the adsorption of Cu2+ by ion exchange. …”
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RETRACTED: A Synthetic Peptide Designed to Neutralize Lipopolysaccharides Attenuates Metaflammation and Diet-Induced Metabolic Derangements in Mice by Shireen Mohammad, Sura Al Zoubi, Sura Al Zoubi, Debora Collotta, Nadine Krieg, Nadine Krieg, Bianka Wissuwa, Bianka Wissuwa, Gustavo Ferreira Alves, Gareth S. D. Purvis, Gareth S. D. Purvis, Giuseppe Danilo Norata, Giuseppe Danilo Norata, Giuseppe Danilo Norata, Andrea Baragetti, Andrea Baragetti, Alberico Luigi Catapano, Alberico Luigi Catapano, Alberico Luigi Catapano, Egle Solito, Egle Solito, Elisabeth Zechendorf, Tobias Schürholz, Wilmar Correa-Vargas, Klaus Brandenburg, Sina M. Coldewey, Sina M. Coldewey, Massimo Collino, Muhammad M. Yaqoob, Lukas Martin, Lukas Martin, Christoph Thiemermann

“…The liver steatosis caused by feeding mice a HFD resulted in the activation of nuclear factor kappa light chain enhancer of activated B cells (NF-ĸB) (phosphorylation of inhibitor of kappa beta kinase (IKK)α/β, IκBα, translocation of p65 to the nucleus), expression of NF-ĸB-dependent protein inducible nitric oxide synthase (iNOS) and activation of the NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome, all of which were reduced by Peptide 19-2.5. …”
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Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

“…The proteinase K gene was inserted into pPICZα-A in such a way that – at a subsequent stage of transfection into yeast cells – it was efficiently expressed under the control of the promoter and terminator of the AOX1 gene, and the product of the exogenous gene contained the signal peptide of the Saccharomyces cerevisiae a-factor to ensure the protein’s secretion into the culture medium. …”
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Characteristics and clinical significance of immune cells in omental milky spots of patients with gastric cancer by Yasunobu Mano, Yasunobu Mano, Yuka Igarashi, Yuka Igarashi, Keisuke Komori, Keisuke Komori, Itaru Hashimoto, Itaru Hashimoto, Hayato Watanabe, Kosuke Takahashi, Kazuki Kano, Hirohito Fujikawa, Takanobu Yamada, Hidetomo Himuro, Hidetomo Himuro, Taku Kouro, Taku Kouro, Feifei Wei, Feifei Wei, Kayoko Tsuji, Kayoko Tsuji, Shun Horaguchi, Shun Horaguchi, Shun Horaguchi, Mitsuru Komahashi, Mitsuru Komahashi, Mitsuru Komahashi, Takashi Oshima, Tetsuro Sasada, Tetsuro Sasada

“…The omentum is a common site of peritoneal metastasis in various cancers, including gastric cancer. It contains immune cell aggregates known as milky spots, which provide a microenvironment for peritoneal immunity by regulating innate and adaptive immune responses. …”
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The impact of prolonged blockade of the releasing hormone on alterations in cardiac macrophage polarisation subsequent to quercetin administration in rats by T.A. Voroshylova, V.I. Shepitko, Ye.V. Stetsuk, N.V. Boruta, O.V. Vilkhova, M.V. Rud, G.S. Puzyryov, O.V. Sych, А.A. Miahkokhlib

“…All cells are subject to changes under the influence of various factors, some of which are induced by macrophages containing CD68 and CD163. The heart is unique in its structure and function, yet it is subject to changes under the influence of various factors. …”
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Association of breast milk microbiota and metabolites with neonatal jaundice by TianYu Liu, TianYu Liu, Yanhan Yuan, Jinying Wei, Jinying Wei, Jiayi Chen, Feng Zhang, Feng Zhang, Juanjuan Chen, Jinping Zhang

“…Breast milk from mothers of healthy newborns contains higher levels of beneficial bacteria and lipid family compared to mothers of newborns with jaundice. …”
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FNDC5/irisin mitigates the cardiotoxic impacts of cancer chemotherapeutics by modulating ROS-dependent and -independent mechanisms by Manish Kumar, Abhishek Singh Sengar, Anushree Lye, Pranesh Kumar, Sukhes Mukherjee, Dinesh Kumar, Priyadip Das, Suvro Chatterjee, Adele Stewart, Biswanath Maity

“…Here, we demonstrate that fibronectin type III domain-containing protein 5 (FNDC5), the precursor protein for myokine irisin, is depleted in the hearts of human cancer patients or mice exposed to chemotherapeutics. …”
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Reductive acetogenesis is a dominant process in the ruminant hindgut by Qiushuang Li, Jiabin Huo, Gaofeng Ni, Fan Zhang, Shizhe Zhang, Xiumin Zhang, Rong Wang, Jinzhen Jiao, Zhongtang Yu, Xuanxuan Pu, Yipeng Yue, Emilio M. Ungerfeld, Xiaoli Zhang, Jian Wu, Zhiliang Tan, Chris Greening, Min Wang

“…Microbial profiling indicated that the cecum harbors a greater abundance of mucin-degrading microbes and fermentative hydrogen producers, whereas the rumen contains a higher abundance of fibrolytic fermentative bacteria, hydrogenotrophic respiratory bacteria, and methanogenic archaea. …”
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Determination and prediction of the apparent and standardized ileal amino acid digestibility in soybean meal fed to the pullets by Sumei Cao, Yuxin Yang, Guangtian Ma, Jing Wang, Kai Qiu, Zhigang Song, Shugeng Wu, Guanghai Qi, Haijun Zhang

“…This experiment was conducted to evaluate the chemical composition, apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acid (AA) in 10 different sources of soybean meal (SBM) fed to the pullets and established prediction equations based on the chemical composition of SBM. 792 Hy Line Brown hens aged 12-15 weeks were divided into 11 diet groups, the diets included one nitrogen-free diet (NFD) used to measure the basal endogenous losses of AA and 10 SBM test diets containing 35.2 % SBM as the sole source of AA. The 0.5 % titanium dioxide (TiO2) was used as an indigestible marker. …”
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Cardiovascular Protection of Nephropathic Male Patients by Oral Food Supplements by Annalisa Noce, Giulia Marrone, Manuela Di Lauro, Silvia Urciuoli, Anna Pietroboni Zaitseva, Georgia Wilson Jones, Nicola Di Daniele, Annalisa Romani

“…The use of an oral food supplement (OFS) containing natural active compounds from Capsicum annuum L., Garcinia cambogia, Centella asiatica L., artichoke, and Aesculus hippocastanum L. which are virtually devoid from side effects, but rich in antioxidant and antiradical properties, could represent a valid therapeutic adjunct in the clinical management of nephropathic patients. …”
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The Beneficial Application of Turmeric (Curcuma longa L.) on Health and Egg Production, in Layers: A Review by Saade Abdalkareem Jasim, Aiman Mohammed Baqir Al‐Dhalimy, Maryam Zokaei, Shadi Salimi, Mohammed Jawad Alnajar, Abhinav Kumar, Enas R. Alwaily, Ahmed Hussein Zwamel, Soura Alaa Hussein, Majid Gholami‐Ahangaran

“…Data on various parameters, including immune function, reproductive performance, egg quality and production parameters, were extracted and analysed. Results Turmeric contains a bioactive compound called curcumin, which possesses antioxidant, anti‐inflammatory, antimicrobial and immunomodulatory effects. …”
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Phytochemical, Cytotoxic, and Antimicrobial Evaluation of the Fruits of Miswak Plant, Salvadora persica L. by Mohammed Al Bratty, Hafiz A. Makeen, Hassan A. Alhazmi, Sohier M. Syame, Ashraf N. Abdalla, Husham E. Homeida, Shahnaz Sultana, Waquar Ahsan, Asaad Khalid

“…The petroleum ether extract of the fruits contained mainly eicosamethyl-cyclodecasiloxane (23.81%), 1-monolinoleoylglycerol (11.78%), (Z,Z,Z)-9,12,15-octadecatrienoic acid ethyl ester derivative (10.56%), and tetracosamethyl-cyclododecasiloxane (9.91%). …”
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Comprehensive analysis reveals cholesterol metabolism-related signature for predicting prognosis and guiding individualized treatment of glioma by Dengfeng Lu, Fei Wang, Yayi Yang, Aojie Duan, Yubo Ren, Yun Feng, Haiying Teng, Zhouqing Chen, Xiaoou Sun, Zhong Wang

“…Results: The prognostic CMRGS contained seven genes: APOE, SCD, CXCL16, FABP5, S100A11, TNFRSF12A, and ELOVL2. …”
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Analysis of the diagnostic efficacy of ultrasound, MRI, and combined examination in benign and malignant breast tumors by Dianpei Ma, Dianpei Ma, Changliang Wang, Jie Li, Jie Li, Xiaohan Hao, Yun Zhu, Zhizhen Gao, Zhizhen Gao, Chun Liu, Changfan Luo, Yu Huang

“…BackgroundTo compare the diagnostic effectiveness of ultrasound (US), magnetic resonance imaging (MRI), and their combined application in distinguishing between benign and malignant breast tumors, with particular emphasis on evaluating diagnostic performance in different breast densities—fatty breast tissue, where fat predominates, and dense breast tissue, which contains a significant amount of fibroglandular tissue.Materials and methodsA retrospective analysis was conducted on 185 patients with breast tumors, including 90 malignant and 95 benign cases. …”
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Single-chain variable fragment affinity tuning can optimize anti-AML CAR-NK cell functionality by Marianna Zahurak, Ravi Varadhan, Ilias Christodoulou, Ruyan Rahnama, Jun Choe, Huilin Yang, Monika Kizerwetter, Christian Guaraca, Natalie Jordan Holl, Stamatia C Vorri, Megan Zinsky, Danielle G Jones, Nikol Garcia Espinoza, Yun-Huai Kuo, Jamie B. Spangler, Challice L. Bonifant

“…Here, we study the functional consequences of CAR binding affinity and targeted epitope on CAR-NK cell activation, cytolytic synapse formation, and antitumor activity.Methods We characterized NK-92 and primary NK cell populations expressing variant affinity AML-specific CARs containing single-chain variable fragments (scFvs, 26292 or 7G3) targeting two epitopes on CD123. 26292 affinity variants were discovered through directed evolution of an error-prone mutagenic library, while 7G3 affinity variants were previously reported. …”
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Uncovering the Hidden Physical Structures and Protostellar Activities in the Low-metallicity S284-RE Region: Results from ALMA and JWST by O. R. Jadhav, L. K. Dewangan, Aayushi Verma, N. K. Bhadari, A. K. Maity, Saurabh Sharma, Mamta

“…ALMA continuum source #2 hosts at least three outflow-driving sources, whereas ALMA continuum source #3 contains two. The bipolar outflow olc1, driven by an embedded object within continuum source #2, is likely a massive protostar, as indicated by the Br- α and PAH emissions depicted in the JWST (F405N+F444W)/F356W ratio map. …”
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Increase in disturbance-induced canopy gaps leads to reorganization of Central European bird communities by Anne Graser, Claudia Frank, Friederike Kunz, Andreas Schuldt, Cornelius Senf, Christoph Sudfeldt, Sven Trautmann, Johannes Kamp

“…We harnessed a unique dataset from a nation-wide bird monitoring scheme containing richness and abundance data from 927 plots monitored annually from 2005 to 2019 across all of Germany (153,014 observations). …”
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