Structure and metabolic function of spatiotemporal pit mud microbiome by Zhihua Li, Chi Zhao, Zhenyu Mao, Fengju Zhang, Ling Dong, Chuan Song, Yao Chen, Xin Fu, Zonghua Ao, Yanfei Xiong, Qin Hui, Weizhi Song, Petri Penttinen, Suyi Zhang

“…We identified 178 medium- and high-quality non-redundant metagenome-assembled genomes (MAGs), and constructed their phylogenetic tree, depicting their roles in the carbon, nitrogen, and sulfur cycles. …”
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Linkage Analysis between Solid-Fluid Coupling and the Strength Reduction Method for Karst Cave Water Inrush in Mines by Yanlin Zhao, Jian Liao, Qiang Liu, Yang Li, Jianchao Cheng

“…Combining the nonlinear seepage-pipe coupling model with the strength reduction method, the linkage analysis of fluid solid coupling and strength reduction method are constructed to study the whole process of confined Karst cave water inrush. …”
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Mixed reality infrastructure based on deep learning medical image segmentation and 3D visualization for bone tumors using DCU-Net by Kun Wang, Yong Han, Yuguang Ye, Yusi Chen, Daxin Zhu, Yifeng Huang, Ying Huang, Yijie Chen, Jianshe Shi, Bijiao Ding, Jianlong Huang

“…Conclusion: The deep learning DCU-Net algorithm model can improve the performance of tumor CT image segmentation, and the reconstructed fine model can better reflect the actual situation of individual tumors; the MR system constructed based on this model enhances clinicians’ understanding of tumor morphology and spatial relationships. …”
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Early risk assessment in paediatric and adult household contacts of confirmed tuberculosis cases by novel diagnostic tests (ERASE-TB): protocol for a prospective, non-interventiona... by Ursula Panzner, Katharina Kranzer, Tsitsi Bandason, Kuda Mutasa, Sandra Rukobo, Charles Sandy, Bariki Mtafya, Andrea Rachow, Norbert Heinrich, Michael Hoelscher, Judith Bruchfeld, Olena Ivanova, Nyanda Elias Ntinginya, Doreen Pamba, Laura Olbrich, Issa Sabi, Simeon Mwanyonga, Elmar Saathoff, Willyhelmina Olomi, Junior Mutsvangwa, Hazel M Dockrell, Edson Tawanda Marambire, Denise Banze, Alfred Mfinanga, Theodora D Mbunda, Khosa Celso, Gunilla Kallenius, Claire J Calderwood, Christof Geldmacher, Kathrin Held, Tejaswi Appalarowthu, Friedrich Rieß, Anna Shepherd, Christopher Sundling, Mishelle Mugava, Martha Chipinduro, Lwitiho Sudi, Antelmo Haule, Emmanuel Sichone, Paschal Qwaray, Harrieth Mwambola, Lilian Minja, Peter Edwin, Dogo Ngalison, Stella Luswema, Celina Nhamuave, António Machiana, Carla Madeira, Emelva Manhiça, Nádia Sitoe, Jorge Ribeiro

“…Statistical analyses will include assessment of diagnostic accuracy by constructing receiver operating curves and calculation of sensitivity and specificity.Ethics and dissemination ERASE-TB has been approved by regulatory and ethical committees in each African country and by each partner organisation. …”
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Development of a latency model for HIV-1 subtype C and the impact of long terminal repeat element genetic variation on latency reversal by Shreyal Maikoo, Robert-Jan Palstra, Krista L. Dong, Tokameh Mahmoudi, Thumbi Ndung'u, Paradise Madlala

“…To address this scientific gap, we constructed a minimal genome retroviral vector expressing HIV-1C consensus transactivator of transcription protein (Tat) and green fluorescent protein (GFP) under the control of either HIV-1C consensus LTR (C731CC) or the transmitted/founder (T/F) LTRs derived from PLWH (CT/F731CC), produced corresponding LTR pseudotyped viruses using a vesicular stomatitis virus (VSV-G) pseudotyped Envelope vector and the pCMVΔR8.91 packaging vector containing HIV-1 accessory and rev genes. …”
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On the tracks of an uninvited guest, the Asian tiger mosquito, Aedes albopictus in Cyprus by Riccardo Piccinno, Giulia Fiorenza, Marlen Ines Vasquez, Jeremy Bouyer, Gregoris Notarides, Ludvik Marcus Gomulski, Soteris Meletiou, Mustafa Akiner, Antonios Michaelakis, Federico Forneris, Giovanni Maga, Giuliano Gasperi, Anna Rodolfa Malacrida

“…Methods We applied highly polymorphic simple sequence repeat (SSR) markers to the Ae. albopictus mosquitoes collected in Cyprus and to specimens from Italy, France, Switzerland, the Balkans, Greece and Turkey to construct an SSR individual genotype dataset that would enable the invasion pattern of Ae. albopictus in Cyprus to be traced. …”
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Remote sensing-based maize growth process parameters revel the maize yield: a comparison of field- and regional-scale by Minghan Cheng, Xiuliang Jin, Chenwei Nie, Kaihua Liu, Tianao Wu, Yuping Lv, Shuaibing Liu, Xun Yu, Yi Bai, Yadong Liu, Lin Meng, Xiao Jia, Yuan Liu, Lili Zhou, Fei Nan

“…These parameters were used to construct a maize yield estimation model applicable at both regional and field scales. …”
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Evaluation of ecological geological environment carrying capacity and analysis of driving mechanisms based on normal cloud model and geodetector model by Rongkun Dai, Changlai Xiao, Xiujuan Liang, Lin Jia, Yi Jia, Jiarui Yao, Weifei Yang, Jiang Zhang, Linzuo Zhang, Weifeng Li

“…In order to realize the sustainable development of the region in this fragile ecological-geological environment, it is necessary to study its Ecological Geological Environmental Carrying Capacity (EGECC) to better serve the regional ecological-geological environment restoration and management work. This study constructs an indicator system encompassing three subsystems: Geological Environment (GE), Social Environment (SE), and Ecological Environment (EE). …”
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DNA hypomethylation-mediated upregulation of GADD45B facilitates airway inflammation and epithelial cell senescence in COPD by Yuan Zhan, Qian Huang, Zhesong Deng, Shanshan Chen, Ruonan Yang, Jiaheng Zhang, Yating Zhang, Maocuo Peng, Jixing Wu, Yiya Gu, Zhilin Zeng, Jungang Xie

“…The effects of GADD45B on inflammation and senescence were investigated via constructing cell model with siRNA transfection or overexpression lentivirus infection and animal model with Gadd45b knockout. …”
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Salt tolerance evaluation and key salt-tolerant traits at germination stage of upland cotton by Mengjie An, Xinhui Huang, Yilei Long, Yin Wang, Yanping Tan, Zhen Qin, Xiantao Ai, Yan Wang

“…In addition, three salt tolerance evaluation models constructed with different variables (6 variables in Model 1; 3 variables in Model 2; 1 variable in Model 3) found that the total fresh weight was the most reliable trait for the salt tolerance evaluation. …”
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Shenshuaikang enema restores the intestinal barrier and microbiota-gut-kidney axis balance to alleviate chronic kidney disease via NF-κB pathway by Yan Ye, Yan Ye, Xiaopeng Huang, Xiaopeng Huang, Xiaopeng Huang, Xueying Li, Xueying Li, Fei Gao, Wenzhen Zhong, Anqi Tang, Anqi Tang, Liangbin Zhao, Dengpiao Xie, Naijing Ye, Naijing Ye

“…NF-κB-RE-Luc transgenic mice were used to construct a CKD mouse model, and changes in fluorescence intensity in mice and isolated kidney tissues were detected within 1–6 days using a small animal live imager. …”
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Altered brain functional network connectivity and topology in type 2 diabetes mellitus by Weiwei Ni, Weiyin Vivian Liu, Mingrui Li, Shouchao Wei, Xuanzi Xu, Shutong Huang, Lanhui Zhu, Jieru Wang, Fengling Wen, Hailing Zhou

“…Post-image preprocessing, brain functional networks were constructed using the Dosenbach atlas and analyzed with the DPABI-NET toolkit through graph theory.ResultsIn T2DM patients, functional connectivity within and between the default mode network (DMN), frontal parietal network (FPN), subcortical network (SCN), ventral attention network (VAN), somatosensory network (SMN), and visual network (VN) was significantly reduced compared to HCs. …”
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Prohexadione-Calcium Reduced Stem and Tiller Damage and Maintained Yield by Improving the Photosynthetic and Antioxidant Capacity of Rice (<i>Oryza sativa</i> L.) Under NaCl Stress by Wanqi Mei, Shaoxia Yang, Jian Xiong, Aaqil Khan, Liming Zhao, Xiaole Du, Jingxin Huo, Hang Zhou, Zhiyuan Sun, Xiaohui Yang, Nana Yue, Naijie Feng, Dianfeng Zheng

“…Prohexadione-calcium (Pro-Ca) has been extensively studied to regulate plant growth, development, and stress responses. However, the constructive role of Pro-Ca in alleviating damages and enhancing rice tillers’ morph-physiological characteristics under salt stress remains largely unknown. …”
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Systemic Overexpression of GDF5 in Adipocytes but Not Hepatocytes Alleviates High-Fat Diet-Induced Nonalcoholic Fatty Liver in Mice by Yan Yang, Wenting Zhang, Xiaohui Wu, Jing Wu, Chengjun Sun, Feihong Luo, Zhou Pei

“…A GDF5 lentiviral vector was constructed, and the LO2 NAFLD cell model was induced by FFA solution (oleic acid and palmitic acid). …”
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Machine learning-based plasma metabolomics for improved cirrhosis risk stratification by Jingru Song, Ziwei Gao, Liqun Lai, Jie Zhang, Binbin Liu, Yi Sang, Siqi Chen, Jiachen Qi, Yujun Zhang, Huang Kai, Wei Ye

“…Metabolomic data were integrated with aspartate aminotransferase to platelet ratio index (APRI) and fibrosis-4 score (FIB-4) to construct predictive models for cirrhosis risk. The model performance was assessed in both the derivation and validation cohorts. …”
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Surface functionalization of titanium implants with controlled-release glutathione conjugate for antioxidant and osteogenesis by Huichuan Xu, Kosuke Nozaki, Ziniu Yu, Nobuyuki Kawashima, Takeshi Ueno, Huaze Wu, Hao Wu, Noriyuki Wakabayashi

“…To counteract intracellular ROS overproduction and the oxidized microenvironment, we constructed vinylphosphonic acid-glutathione (VPA-GSH) conjugates synthesized through a thiol-ene click reaction on Ti substrates, endowing them with good biocompatibility, antioxidant capability, and promotion of osteogenesis. …”
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The Epidemiological Characteristics of Coronavirus Disease (COVID-19) in Halabja Province/Kurdistan –Iraq by Osama Hamid Shareef, Othman Abdulrahman Mohammed, Dashty Aziz Ghafor, Ari Shareef Ali, Hardi Rafat Baqi, Ali Aziz Ali, Aryan Mahmood Faraj, Syamand Ahmed Qadir, Ali Muhi Aldeen Omar Aljabary

“…This study was designed, when the Wafa Hospital constructed in Halabja/Kurdistan region for infected patients. …”
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Establishment of a rapid and quantitative method for detecting the range of infection exposure in preclinical dental education by Ayaka Ueda, Yuki Shinoda-Ito, Kazu Takeuchi-Hatanaka, Takashi Ito, Shintaro Ono, Kimito Hirai, Kazuhiro Omori, Tadashi Yamamoto, Shogo Takashiba

“…Conclusions An education system for infection control constructed using artificial saliva containing Lactobacillus and a UV-sensitive fluorescent hand lotion quantified the infectious areas and degrees. …”
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Bank collapse-landslide failure mechanism of typical reverse rock bank slope in Three Gorges Reservoir area by Yang LI, Hailin ZHAO, Bolin HUANG, Honglin LI, Kaikai XU

“…To further analyze the failure mechanism and disaster process of bank collapse-landslide under the constraint condition of bank collapse, this study constructed a two-dimensional model of Gongjiafang landslide in Wuxia section of Three Gorges Reservoir area to analyze the failure mechanism of bank collapse-landslide of typical reverse rock bank slope under the erosion of reservoir water level. …”
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The Patient-Reported Outcomes Thermometer–5-Item Scale (5T-PROs): Validation of a New Tool for the Quick Assessment of Overall Health Status in Painful Rheumatic Diseases by Fausto Salaffi, Marco Di Carlo, Marina Carotti, Sonia Farah

“…Objective. To investigate the construct validity, reliability (internal consistency and retest reliability), and feasibility of the patient-reported outcomes thermometer–5-item scale (5T-PROs), a new tool to measure overall health status in patients with painful chronic rheumatic diseases such as rheumatoid arthritis (RA), psoriatic arthritis (PsA), axial spondyloarthritis (axialSpA), and fibromyalgia (FM). …”
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