A novel de novo GABRA2 gene missense variant causing developmental epileptic encephalopathy in a Chinese patient by Li Yang, Xingyu Wan, Ran Hua, Junhong Jiang, Baotian Wang, Rui Tao, De Wu

“…A mutant plasmid was constructed and transfected into 293 T cells. Western blotting (WB) was used to evaluate mutant protein expression, while co‐immunoprecipitation (Co‐IP) analyzed interactions with GABRB3 and GABRG2 proteins. …”
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GM-CSF and IL-21-armed oncolytic vaccinia virus significantly enhances anti-tumor activity and synergizes with anti-PD1 immunotherapy in pancreatic cancer by Yujing Xuan, Wenyi Yan, Ruimin Wang, Xibin Wang, Yu Guo, Huilin Dun, Ziyan Huan, Lihua Xu, Ruxia Han, Xianlei Sun, Lingling Si, Nicholas R. Lemoine, Nicholas R. Lemoine, Yaohe Wang, Yaohe Wang, Pengju Wang

“…Oncolytic viruses armed with immunoregulatory molecules are promising strategies to overcome limited efficacy and target inaccessible and metastatic tumors. In this study, we constructed a tumor-selective vaccinia virus (VV) with deletions of the TK and A49 genes (VVLΔTKΔA49, VVL-DD) using CRISPR-Cas9-based homologous recombination. …”
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Deep Ensemble Learning for Human Action Recognition in Still Images by Xiangchun Yu, Zhe Zhang, Lei Wu, Wei Pang, Hechang Chen, Zhezhou Yu, Bin Li

“…In this research, we investigate human action recognition in still images and utilize deep ensemble learning to automatically decompose the body pose and perceive its background information. Firstly, we construct an end-to-end NCNN-based model by attaching the nonsequential convolutional neural network (NCNN) module to the top of the pretrained model. …”
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Comprehensive geological analysis and evaluation of the feasibility of renovating the Wanshunchang gas reservoir into a gas storage facility by Zebin Tong, Zhonggui Hu, Longwei Qiu, Na Zhang, Huayin Zhu, Daoyong Zhou, Gang Zhou, Xiao Chen, Yanlin Shao, Jiuzhen Hu

“…Abstract As many oil and gas reservoirs approach depletion stages in the future, alongside growing energy storage demands, constructing gas storage facilities becomes critical for ensuring a stable natural gas supply. …”
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Red Stellar Populations and Dust Extinction toward W3 by J. L. Campbell, P. G. Martin, S. Song, M. Rahman, L. Einstein

“…We use a Rayleigh–Jeans color excess method modified to de-redden stellar observations of both red giants (RGs) and OB stars, and construct an IR Hertzsprung–Russell diagram validated against the Besançon Galactic model. …”
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Finding mobility in place attachment research: lessons for managed retreat by Robin Willcocks-Musselman, Robin Willcocks-Musselman, Julia Baird, Karen Foster, Julia Woodhall-Melnik, Kate Sherren

“…Research at the intersection of place attachment and managed retreat is limited, partially because these are complicated constructs, each with confusing terminologies. By viewing the concept of managed retreat as a form of mobility-based climate adaptation, this paper attempts to gain insights from other mobility-related fields. …”
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p54-Fc-Labeled Gold Nanoparticle-Based Lateral Flow Strip-Assisted Portable Devices for Rapid and Quantitative Point-of-Care Detection of ASFV Antibodies by Yang Yang, Yuhao Li, Ziyang Wang, Minglong Tong, Pengcheng Zhu, Juanxian Deng, Zongjie Li, Ke Liu, Beibei Li, Donghua Shao, Zhongren Zhou, Yafeng Qiu, Zhiyong Ma, Jianchao Wei

“…Additionally, it introduces a new approach for constructing IC test strips using SPA protein without antibody preparation, making it a reliable on-site antibody test for ASFV.…”
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Identification of depressive symptoms in adolescents using machine learning combining childhood and adolescence features by Xinzhu Liu, Rui Cang, Zihe Zhang, Ping Li, Hui Wu, Wei Liu, Shu Li

“…Using the maximum relevance-minimum redundancy method and four ML algorithms, we determined the optimal feature subset for identifying depressive symptoms and constructed child-adolescent models. Stepwise logistic regression and four ML methods were employed to create demographic and combined models, respectively. …”
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Choosing the most suitable NGS technology to combine with a standardized viral enrichment protocol for obtaining complete avian orthoreovirus genomes from metagenomic samples by Sonsiray Álvarez-Narváez, Sonsiray Álvarez-Narváez, Telvin L. Harrell, Islam Nour, Sujit K. Mohanty, Steven J. Conrad

“…The custom reference genomes were in turn constructed with the publicly available ARV genomic segments that shared the highest sequence similarity with the contigs from short-read de novo assemblies. …”
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Efficient Acquisition of Fully Human Antibody Genes against Self-Proteins by Sorting Single B Cells Stimulated with Vaccines Based on Nitrated T Helper Cell Epitopes by Liangliang Jiang, Tao Jiang, Jianhua Luo, Yanliang Kang, Yue Tong, Xiaoda Song, Xiangdong Gao, Wenbing Yao, Hong Tian

“…However, owing to immune tolerance to autoantigen, human autoantigen-specific B cells are difficult to acquire by conventional single B cell technology. In this study, we constructed a nitrated T-cell epitope named NitraTh by incorporating p-nitrophenylalanine into a universal T helper epitope. …”
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Systematic analysis of co-expression network and gene expression profile of differentially expressed lncRNA-mRNA one week after spinal cord injury by Yi Kang, Yijie Song, Zhongju Shi, Qiang Li, Yigang Lv, Mengfan Hou, Cui Ding, Jing Xie, Yongfu Lou, Chi Zhang, Huan Jian, Xueying Li, Hengxing Zhou, Shiqing Feng

“…Gene Ontology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of DE lncRNAs and mRNAs were subsequently carried out to explore crucial biological processes and signaling pathways. Moreover, we constructed protein–protein interaction (PPI) networks, the STRING database, and identified the 10 high degree core nodes. …”
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Understanding sociodemographic differences in climate behavior, climate policy acceptance, and political participation by Lena Lehrer, Lennart Hellmann, Cornelia Betsch

“…Additional analyses explore the relations between age and the constructs at item level. Conclusions: Findings underscore the importance of a nuanced understanding of people's readiness to act against climate change. …”
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Study on the Regulation Effect of Optogenetic Technology on LFP of the Basal Ganglia Nucleus in Rotenone-Treated Rats by Zongya Zhao, Yanxiang Niu, Peiqi Chen, Yu Zhu, Liangliang Shi, Xuewei Zhao, Chang Wang, Yehong Zhang, Zhixian Gao, Wenshuai Jiang, Wu Ren, Renjun Gu, Yi Yu

“…Rotenone was injected stereotactically into the substantia nigra compact area and ventral tegmental area of SD rats to construct rotenone-treated rats. Then, for the optogenetic manipulation, we injected adeno-associated virus expressing channelrhodopsin to stimulate the globus pallidus and the striatum with a 1 mW blue light and collected LFP signals before, during, and after light stimulation. …”
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Cost-effectiveness analysis of pembrolizumab plus chemotherapy versus chemotherapy in untreated advanced pleural mesothelioma in the Chinese healthcare system by Wenwang Lang, Yulong He, Changchun Hou, Hua Li, Qinling Jiang, Liuyong Mei

“…The purpose of this study was to assess its cost-effectiveness.Materials and methodsA Markov state-transition model was constructed using data from the IND227 phase 3 randomized clinical trial. …”
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Validating a novel measure for assessing patient openness and concerns about using artificial intelligence in healthcare by Bryan A. Sisk, Alison L. Antes, Sunny C. Lin, Paige Nong, James M. DuBois

“…We conducted a confirmatory factor analysis and assessed reliability and construct validity. We performed exploratory multivariable regression models to identify predictors of openness. …”
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GUARANTEEING THE ACCES OF THIRD PARTIES TO THE NETWORK INDUSTRIES SPECIFIC INFRASTRUCTURES – A VITAL COMPONENT OF EU'S POLICY IN THE FIELD OF COMPETITION ON THE ENERGY MARKET, IN T... by Iulian BĂICULESCU, Dragoș-Adrian BANTAȘ

“…As both the practical experience and the theoretical constructions of successive generations of practitioners and researchers of the phenomenon of the economy and the legal norms associated with its proper functioning have proven, the most important mechanism for increasing consumer satisfaction in a market, both from the perspective of the quality of the goods and services they benefit from, as well as from that of the prices demanded, is the competition. …”
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Genetic structure of the collection of ryegrass (<i>Lolium</i>) cultivars: a study based on SSR and SCoT markers by Yu. M. Mavlyutov, E. A. Vertikova, A. O. Shamustakimova, I. A. Klimenko

“…Based on PCoA, carried out using summarized data of SSR and SCoT analyses, a multidimensional diagram of genetic relationships among ryegrass cultivars was constructed.Conclusions. The systems of SSR and SCoT markers appeared to be an efficient tool to reveal genetic polymorphism and identify differences among ryegrass species and cultivars. …”
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Machine learning and SHAP value interpretation for predicting comorbidity of cardiovascular disease and cancer with dietary antioxidants by Xiangjun Qi, Shujing Wang, Caishan Fang, Jie Jia, Lizhu Lin, Tianhui Yuan

“…Feature preprocessing included removing collinear features, addressing class imbalance, and normalizing data. Models constructed within the mlr3 framework included recursive partitioning and regression trees, random forest, kernel k-nearest neighbors, naïve bayes, and light gradient boosting machine (LightGBM). …”
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A New Approach to Identifying Red Supergiant Stars in Metal-poor Galaxies: A Case Study of NGC 6822 by Zhi-wen Li, Ming Yang, Biwei Jiang, Yi Ren

“…This work attempts to improve the CCD method in combination with the Gaia astrometric measurement to remove foreground contamination in order to construct a complete RSG sample in metal-poor galaxies. …”
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Encoding of cerebellar dentate neuron activity during visual attention in rhesus macaques by Nico A Flierman, Sue Ann Koay, Willem S van Hoogstraten, Tom JH Ruigrok, Pieter Roelfsema, Aleksandra Badura, Chris I De Zeeuw

“…To better understand the heterogeneous relationship between task structure, behavioral performance, and neural dynamics, we constructed a behavioral, an encoding, and a decoding model. …”
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