Genomic Analysis of miR-21-3p and Expression Pattern with Target Gene in Olive Flounder by Ara Jo, Hee-Eun Lee, Heui-Soo Kim

Subjects: “…conserved sequence…”
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Rational Primer and Probe Construction in PCR-Based Assays for the Efficient Diagnosis of Drifting Variants of SARS-CoV-2 by Divya RSJB Rana, Nischal Pokhrel, Santosh Dulal

“…Hence, the construction of primers and probes with the rational selection of targeting specific E gene, genomic regions with highly conserved sequences, multiple target genes with relatively lower mutability and detectable level of concentration, SNP-specific binding regions of spike (S gene) protein, and shorter amplicon size (100–150 bp) are vital for the PCR assays to achieve optimal efficiency in the point-of-care laboratory diagnosis of circulating drifting variants of SARS-CoV-2 with optimal accuracy.…”
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Duplex qPCR for detecting and differentiating porcine epidemic diarrhea virus GI and GII subtypes by Bin Wang, Wang Han, Di Wu, Yue Jing, Li Ma, Feiyang Jiang, Shusen Ji, Lianmei Bai, Xiuju Yu, Wei Hou, Shouyu Wang, Haidong Wang

“…PCR is the most commonly used technique for PEDV diagnosis in practical clinics, however, reported works still suffer from shortcomings, for example, most of them cannot differentiate GI and GII subtypes, they suffer from low sensitivity, and some primer sequences are no longer able to match the mutant strains.MethodsTo address these issues, we conducted a comprehensive analysis by comparing the sequences of the PEDV S protein in the existing NCBI database with a recently isolated epidemic strain of PEDV, named SX0818-2022, of subtype GIIa from Shanxi, China. The conserved sequences of GI and GII subtypes were retrieved to design the primers and probe. …”
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Research note: Establishment of a multiplex PCR detection system for different infectious bronchitis virus strain genotypes in laying hens by Changjing Zhao, Cheng Yuan, Qingsen Zhao, Aihong Xia, Yaqing Dong, Shuying Xu, Shihan Chen, Yue Yuan, Yongjuan Wang

“…Based on the spike 1 (S1) glycoprotein gene sequences of the IBV Mass, QX, G Ⅵ-1, 4/91, and LDT3 strains, five sets of customized primers, showing excellent specificity, were designed and synthesized to target the appropriate conserved sequences. After repeated improvement, a multiple PCR detection approach with strong specificity for the simultaneous detection of IBV Mass, QX, G Ⅵ-1, 4/91, and LDT3 strains was initially established. …”
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Characterization of β-Actin Promoter from Nile Tilapia (Oreochromis niloticus) by . Alimuddin, A. Octavera, O.Z. Arifin, K. Sumantadinata

“…The result of sequence analysis showed that the length of DNA fragment obtained is about 1.5 kb and containing the evolutionary conserved sequences of transcription factor for β-actin promoter including CCAAT, CArG and TATA boxes.  …”
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pH-induced conformational changes in the selectivity filter of a potassium channel lead to alterations in its selectivity and permeation properties by Carlos Coll-Díez, Ana Marcela Giudici, Alberto Potenza, José Manuel González-Ros, José Antonio Poveda

“…The Selectivity Filter (SF) in tetrameric K+ channels, has a highly conserved sequence, TVGYG, at the extracellular entry to the channel pore region. …”
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Genome constitution and differentiation of subgenomes in Siberian and Far Eastern endemic species of the genus Elymus (Poaceae) according to the sequencing of the nuclear gene waxy by A. V. Agafonov, S. V. Asbaganov, E. V. Shabanova (Kobozeva), I. V. Morozov, A. A. Bondar

“…Instead of this deletion, all St subgenomes have a relatively conservative sequence similar to that of the genus Pseudoroegneria, whose ancestor is considered to be the donor of the modern St subgenome for all Elymus species. …”
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