彗星试验检测大鼠死后肾脏细胞DNA含量与死亡时间的关系

“…【目的】 探讨特定条件下,利用彗星试验测定SD大鼠死后6 ~ 48 h肾脏细胞DNA含量与死亡时间的关系65377;【方法】 健康成年雌性SD大鼠,按取材时间随机分为6组,每组3只65377;颈椎脱臼法处死动物,置于25.1 ℃(广州地区近5年10月份平均气温)培养箱中,分别于065380;665380;1265380;2465380;3665380;4865380;60 h取大鼠肾脏组织,制备单细胞悬液,进行彗星试验,荧光显微成像系统采集图像,采集相关参数(Comet 4.0)并进行Kruskal-Wallis检验65377; 【结果】 死后6 ~ 48 h, SD大鼠肾脏细胞DNA碎片随死亡时间延长而增加,尾长在死后各时间点依次呈明显的增长趋势,Oliver尾矩和尾DNA随死亡时间的延长,亦有一定的增长趋势,60 h未能测出65377;Kruskal-Wallis检验显示:各时间点彗星尾长均数的差异有高度显著性(P < 0.01);彗星尾矩均数在死后6 h和12 h差异无统计学意义(P > 0.05),24 h和36 h彗星尾矩均数差异有显著性(P < 0.05);尾DNA均数在6 h,12 h,24 h三个时间点差异无显著性(P > 0.05),但与36 h,48 h尾DNA均数的差异均有显著性(P < 0.05)65377;【结论】 SD大鼠肾脏细胞DNA降解随死亡时间延长而增加,彗星试验技术为死亡时间推断提供了重要的实验依据65377;…”
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Insecticide chlorfenapyr confers induced toxicity in human cells through mitochondria-dependent pathways of apoptosis by Longfei Wang, Zheng Qu, Yifan Xu, Guangqing Yu, Xiangyang Liu, Meizi Wang, Shiheng An, Xinming Yin, Risong Na, Youwu Hao

“…Chl's ability to induce DNA damage and apoptosis in human cells was confirmed through alkaline comet assay, immunofluorescence assay, and flow cytometric analysis. …”
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Assessment of the methodological quality of studies on core outcome sets for respiratory diseases: A systematic review and meta-research study. by Mengjuan Liu, Jiajia Wang, Lu Wang, Xinyi Zhang, Ruiyu Hao, Duolao Wang, Tao Chen, Jiansheng Li

“…<h4>Methods</h4>PubMed, Embase, Cochrane Library, and Web of Science were searched for study protocols or original studies on COS-RD about adults, from their inception to February 23, 2024. The COMET database and Chinese databases (including China National Knowledge Infrastructure, Wanfang Data, Chongqing VIP database, and China Biology Medicine) were also searched as a supplement. …”
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Molecule interacting with CasL-2 enhances tumor progression and alters radiosensitivity in cervical cancer by Yun Teng, Hongmei Zhao, Guoqing Xue, Guohui Zhang, Yanbin Huang, Wei Guo, Kun Zou, Lijuan Zou

“…DNA damage in irradiated cells with MICAL2 knockdown or overexpression was evaluated using the comet assay, while γ-H2AX and Rad51 protein levels were detected by WB. …”
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Investigating the optimal maximum diameter of benign thyroid nodules for thermal ablation on the basis of complete disappearance rate by Shi-Liang Cao, Wan-Ying Shi, Zhen-Long Zhao, Ying Wei, Na Yu, Jie Wu, Li-Li Peng, Yan Li, Ming-An Yu

“…The MD was significantly negatively correlated with complete disappearance (odds ratio 0.89, 95% confidence interval 0.87–0.92; p < 0.001). Calcification, comet-tail artifacts, multilocular cysts, and composition of BTNs, as well as diabetes were negatively correlated with complete disappearance. …”
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Estimation of median LC50 and toxicity of environmentally relevant concentrations of thiram in Labeo rohita by Sana Alam, Gulnaz Afzal, Riaz Hussain, Hafiz Muhammad Ali, Amtul Sami, Rabbiah Manzoor Malik, Raheela Jabeen, Farid Shokry Ataya, Kun Li

“…Oxidative stress parameters; ROS and TBARS were increased significantly (p < 0.05) while antioxidant enzymes (CAT, SOD, POD, GSH) showed a significant (p < 0.05) decrease in the treated groups compared to the control. Moreover, comet assay revealed significant (p < 0.05) DNA damage in the isolated cells of kidneys, heart and brain at higher doses (80 μg/L and 120 μg/L) of thiram that was further confirmed by histo-pathological alterations in these tissues. …”
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Reproductive Toxicity of Theobroma cacao: Increase in Survival Index, Nongenotoxic, and Proimplantation Potential by I. J. Asiedu-Gyekye, T. G. Borovskaya, M. E. Poluektova, А. V. Vychuzhanina, Y. А. Shchemerovа, S. I. Kamalova, V. A. Grgoreva, P. Amoateng, K. E. Kukuia, A. A. Kwapong, L. Allotey Babington, S. K. Amponsah, B. B. N’guessan

“…A preliminary genotoxic potential was evaluated by the DNA comet assay test using C57Bl/6 mice. Both therapeutic dose (TD; 900 mg/kg) and high dose (HD; 9000 mg/kg) of UNCP were used. …”
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Anticancer activity of Ilex khasiana, a rare and endemic species of holly in Northeast India, against murine lymphoma by Charles Lalnunfela, Pawi Bawitlung Lalthanpuii, Hmar Tlawmte Lalremsanga, Zothansiama, Chhaihlo Lalmuansangi, Mary Zosangzuali, Nachimuthu Senthil Kumar, Tochhawng Lalhriatpuii, Kholhring Lalchhandama

“…On the other hand, glutathione level, glutathione S-transferase and superoxide dismutase activity increased. Alkaline comet assay showed that the plant extract effectively induced DNA damage, producing a tail length of 11.89 μm and Olive moment of 2.36 at 250 mg/kg bwt, the most effective dosage. …”
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Beyond Chemistry: Investigating the Physical, Pharmacological, and Computational Aspects of Polyoxometalate Integrated Solid Lipid Nanoparticles for Cancer Treatment by Khan R, Tulain UR, Shah HS, Usman F, Chohan TA, Iqbal J, Kazi M, Ijaz M, Erum A, Malik NS, Mahmood A

“…Further examinations of DNA damage were made through comet test and flow cytometry techniques. The assessment of tumor regression and survival was conducted, and comparison was recorded. …”
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Genotoxicity Revaluation of Three Commercial Nitroheterocyclic Drugs: Nifurtimox, Benznidazole, and Metronidazole by Annamaria Buschini, Lisa Ferrarini, Susanna Franzoni, Serena Galati, Mirca Lazzaretti, Francesca Mussi, Cristina Northfleet de Albuquerque, Tânia Maria Araújo Domingues Zucchi, Paola Poli

“…The compounds are more active for base-pair substitution than frameshift induction in Salmonella; NFX and BNZ are more mutagenic than MTZ; they are widely dependent from nitroreduction whereas microsomal fraction S9 weakly affects the mutagenic potential. Comet assay detects BNZ- and NFX-induced DNA damage at doses in the range of therapeutically treated patient plasma concentration; BNZ seems to mainly act through ROS generation whereas a dose-dependent mechanism of DNA damaging is suggested for NFX. …”
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Calcium hydroxide nanoparticles induce cell death, genomic instability, oxidative stress and apoptotic gene dysregulation on human HepG2 cells by Hanan R. H. Mohamed, Esraa H. Ibrahim, Shahd E. E. Shaheen, Nesma O. E. Hussein, Ayman Diab, Gehan Safwat

“…Cell viability and genomic DNA stability were assessed using the Sulforhodamine B (SRB) assay and alkaline comet assay, respectively. Reactive oxygen species (ROS) levels were measured using 2,7-dichlorofluorescein diacetate, while the expression level of apoptosis-related genes (p53, Bax, and Bcl-2) were quantified using real-time PCR (qRT-PCR). …”
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Apoptosis induction and tumor growth suppression by hydroxyapatite nanoparticles–cisplatin combined treatment in Ehrlich solid carcinoma-bearing mice by Shaymaa M. Eissa, Asmaa M. Mahfouz, Saad M. El-Gendy, Al-shimaa Zakaria, Heba Effat, Hanan R. H. Mohamed

“…DNA damage assessment was performed using comet assay in ESC, in addition, we measured the expression of the following genes (P53, Bcl2, and Bax,) using quantitative real-time PCR, and the apoptotic-related proteins (P53 and Ki-67) using immunohistochemical analysis. …”
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Microwave hyperthermia enhances radiosensitization by decreasing DNA repair efficiency and inducing oxidative stress in PC3 prostatic adenocarcinoma cells by Yajun Wu, Pengyuan Liu, Wendy Chen, Shiting Bai, Sisi Chen, Jianglin Chen, Xiaogang Xu, Jindan Xia, Yufei Wu, Jianjun Lai, Chuan Sun, Zhenghong Lao, Xiaoqing Wan, Zhibing Wu

“…We evaluated PC3 cell proliferation using CCK-8, colony experiments, DAPI staining, comet assay and ROS detection method. We also monitored nude mouse models of PCa during treatment, measured tumor weight, and calculated the tumor inhibition rate. …”
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Molecular Characterization, Oxidative Stress-Mediated Genotoxicity, and Hemato-Biochemical Changes in Domestic Water Buffaloes Naturally Infected with <i>Trypanosoma evansi</i> Und... by Waqas Ahmad, Muhammad Yasin Tipu, Muti ur Rehman Khan, Haroon Akbar, Aftab Ahmad Anjum, Muhammad Ovais Omer

“…Infected animals exhibited significant (<i>p</i> < 0.05) alterations in oxidative stress biomarkers, including catalase, nitric oxide, and malondialdehyde concentrations. Noteworthily, a comet assay revealed a significantly (<i>p</i> < 0.0001) higher mean genetic damage index in the infected buffaloes (0.7 ± 0.04) compared with the healthy ones (0.196 ± 0.004). …”
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Combinatorial functionomics identifies HDAC6-dependent molecular vulnerability of radioresistant head and neck cancer by Sharon Pei Yi Chan, Celestia Pei Xuan Yeo, Boon Hao Hong, Evelyn Mui Cheng Tan, Chaw Yee Beh, Eugenia Li Ling Yeo, Dennis Jun Jie Poon, Pek Lim Chu, Khee Chee Soo, Melvin Lee Kiang Chua, Edward Kai-Hua Chow

“…Drug targeting was validated by western blot analyses, and the Comet assay was used to quantify DNA damage. Enhanced histone deacetylase inhibitor (HDACi) efficacy in RR models was further examined by in vivo studies, and genetic and chemical inhibition of major Class I/II HDACs. …”
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Tousled-like kinase loss confers PARP inhibitor resistance in BRCA1-mutated cancers by impeding non-homologous end joining repair by Min-ah Kim, Banseok Kim, Jihyeon Jeon, Jonghyun Lee, Hyeji Jang, Minjae Baek, Sang-Uk Seo, Dongkwan Shin, Anindya Dutta, Kyung Yong Lee

“…Methods Cellular DSBs were assessed using neutral comet assays and phospho-H2AX immunoblotting. Fluorescence-based reporter assays quantified repair via NHEJ or HR. …”
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In Situ Phytoremediation of Mine Tailings with High Concentrations of Cadmium and Lead Using <i>Dodonaea viscosa</i> (Sapindaceae) by Luis Fernando Acosta-Núñez, Patricia Mussali-Galante, María Luisa Castrejón-Godínez, Alexis Rodríguez-Solís, Joel Daniel Castañeda-Espinoza, Efraín Tovar-Sánchez

“…Also, the effects of cadmium (Cd) and lead (Pb) exposure in <i>D. viscosa</i> were analyzed via DNA damage (comet assay) morphological and physiological characters in exposed <i>vs</i> non-exposed individuals. …”
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The origin and transmission of HIV-1 CRF80_0107 among two major first-tier cities in China by Xiaorui Wang, Bo Zhu, Hanping Li, Jingwan Han, Xiaolin Wang, Lei Jia, Bohan Zhang, Jingyun Li, Linding Wang, Yongjian Liu, Hongling Wen, Lin Li

“…Methods With the partial pol region fragment of the HIV-1 CRF80_0107 subtype standard sequence as a reference, BLAST was used to search for highly similar sequences in the Los Alamos HIV Sequence Database, followed by preliminary subtype identification via COMET. Further phylogenetic and recombination breakpoint analyses were conducted to verify the subtypes and recombination patterns. …”
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Titin gene mutations enhance radiotherapy efficacy via modulation of tumour immune microenvironment in rectum adenocarcinoma by Hengchang Liu, Jialiang Liu, Xu Guan, Zhixun Zhao, Pu Cheng, Haipeng Chen, Zheng Jiang, Xishan Wang

“…Flow cytometry, immunofluorescence, and comet assays examined mutations' impact on cell cycle, apoptosis, and DNA damage response (DDR). …”
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Violencia de pareja durante el enamoramiento en adolescentes. by Diana Quispe Aguilar

“…Conclusión:  gran parte de los adolescentes comete y sufre violencia de nivel bajo, sobre todo de tipo verbal emocional. …”
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