Application of Purified Botulinum Type A Neurotoxin to Treat Experimental Trigeminal Neuropathy in Rats and Patients with Urinary Incontinence and Prostatic Hyperplasia by Yoshizo Matsuka, Teruhiko Yokoyama, Yumiko Yamamoto, Tomonori Suzuki, Ni Nengah Dwi Fatmawati, Atsushi Nishikawa, Tohru Ohyama, Toshihiro Watanabe, Takuo Kuboki, Atsushi Nagai, Keiji Oguma

“…Type A neurotoxin (NTX) of Clostridium botulinum was purified by a simple procedure using a lactose gel column. The toxicity of this purified toxin preparation was retained for at least 1 year at −30°C by supplementation with either 0.1% albumin or 0.05% albumin plus 1% trehalose. …”
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Variation in the Lipid Profile of Pacific Oyster ( Crassostrea gigas ) Cultured in Khanh Hoa Coast, Vietnam, Based on Location and Harvest Period by Minh Van Nguyen, Derrick Kakooza, Anh Phuong Thi Tran, Vy Thao Thi Tran

“…Lipids from C. gigas were separated into neutral and polar lipid fractions using silica gel column chromatography. The amount of neutral lipids recovered from the oyster muscle was significantly higher than that of the polar lipids. …”
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Environmental sustainability profiles assessment of HPLC stability indicating method for quantitation of piracetam and vincamine in pharmaceutical medications by Samya Sh. Alenezi, Ayman A. Gouda, Ragaa El Sheikh, Niaf A. Badahdah, Muneer E Alzuhiri, Ali H. Magrabi, Asim L. Jilani, Ahmed S. Darwish

“…Piracetam, vincamine, and their breakdown products were successfully separated using the isocratic elution technique, and both medicinal compounds' quantification was carried out on the Kromasil C18 column at room temperature. The mobile phase consists of 0.05 M potassium dihydrogen phosphate buffer pH 3.5:ethanol absolute (60:40, by volume), at a flow rate of 1.0 ml/min. …”
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Structural Characterization of Foxtail Millet (<i>Setaria italica</i>) Polysaccharides and Evaluation of Its Antioxidant and Immunostimulatory Activities by Haiying Zhang, Chengyu Peng, Wei Zhang, Huatao Liu, Xiaodong Liu, Changqing Sun, Xiaoning Cao

“…A water-soluble polysaccharide from foxtail millet (FM-D1) was isolated and purified through gradient ethanol precipitation and column chromatography. Size-Exclusion Chromatography–Multi-Angle Light Scattering–Refractive Index (SEC-MALLS-RI) and high-performance anion-exchange chromatography (HPAEC) analyses revealed that FM-D1 constitutes a highly purified neutral polysaccharide exclusively composed of glucose as the sugar unit, with a molecular weight of 14.823 kDa. …”
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Simultaneous production during biogas upgrading: Foundational insights from numerical simulation of dual-reflux pressure swing adsorption by Mohd Hardyianto Vai Bahrun, Awang Bono, Norasikin Othman, Muhammad Abbas Ahmad Zaini

“…The behavior of the DR-PSA is described through pressure and temperature profiles within the bed column at cyclic steady-state conditions. The results indicate that, under preliminary unoptimized conditions, 86.0% of CH4 could be recovered with a purity of 85.8% as a light product, whereas CO2 enriched to 82.9% was achievable as a heavy product, with a recovery of 82.9%, using a pressure ratio, PH/PL of 5. …”
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Satellite-based Emission Inventory Adjustments Improve Simulations of Long-range Transport Events by Wei-Syun Huang, Stephen M. Griffith, Yi-Chun Lin, Ying-Chieh Chen, Chung-Te Lee, Charles C.-K. Chou, Ming-Tung Chuang, Sheng-Hsiang Wang, Neng-Huei Lin

“…This study adjusts a bottom-up emission inventory based on changes in remotely sensed NO2 column densities for a source region of East Asia, then with CMAQv5.2.1 simulates transport of LRT plumes to Taiwan. …”
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Development and Application of a LC-MS/MS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum by Yunliang Zheng, Nana Xu, Xingjiang Hu, Qiao Zhang, Yanpeng Liu, Qingwei Zhao

“…Chromatographic separation was achieved on XSelecT HSS T3 column by gradient elution with a total run time of 5.0 min. …”
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Seasonal phytochemical study and antimicrobial potential of Vetiveria zizanioides roots by Dos Santos Delair Silveira, Oberger Júlia Vieira, Niero Rivaldo, Wagner Theodoro, Delle Monache Franco, Cruz Alexandre Bella, Martin-Quintal Zhelmy, Cechinel Filho Valdir

“…Khusimol (2) was isolated by column chromatography and identified by NMR and CG-MS, along with other sesquiterpenes, including β-vetivenene (1), vetiselinenol (3), isovalencenol (4), vetivenic acid (5), α-vetivone (6) and β-vetivone (7). …”
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Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method by Giuseppina Nocca, Cinzia Callà, Stefano Angelo Santini, Adriana Amalfitano, Luca Marigo, Diana Valeria Rossetti, Gianrico Spagnuolo, Massimo Cordaro

“…The analysis was carried out using a JASCO HPLC system with a C-18 (3 μm) Supelco reversed phase column (150 x 4.7 mm) using a mobile phase of 80% CH3OH and 20% of CH3CN: tetrahydrofuran: water (10:80:10, v/v/v). …”
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Vertical Profiles and Chemical Properties of Aerosol Particles upon Ny-Ålesund (Svalbard Islands) by B. Moroni, S. Becagli, E. Bolzacchini, M. Busetto, D. Cappelletti, S. Crocchianti, L. Ferrero, D. Frosini, C. Lanconelli, A. Lupi, M. Maturilli, M. Mazzola, M. G. Perrone, G. Sangiorgi, R. Traversi, R. Udisti, A. Viola, V. Vitale

“…Detailed analysis of two case studies reveals significant differences in composition despite the similar structure (layering) and the comparable texture (grain size distribution) of particles in the air column. Differences in the mineral chemistry of samples point at both local (plutonic/metamorphic complexes in Svalbard) and remote (basic/ultrabasic magmatic complexes in Greenland and/or Iceland) geological source regions for dust. …”
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HPLC-MS/MS-Mediated Analysis of the Pharmacokinetics, Bioavailability, and Tissue Distribution of Schisandrol B in Rats by Dahu Liang, Zijing Wu, Yanhao Liu, Chao Li, Xianghong Li, Bin Yang, Haitang Xie, Hua Sun

“…A liquid-liquid extraction (LLE) approach was employed for the preparation of samples that were subsequently separated with an Agilent ZORBAX Eclipse XDB-C18 (4.6 × 150 mm, 5 μm) column with an isocratic mobile phase consisting of methanol and water containing 5 mM ammonium acetate and 0.1% formic acid (90 : 10, v/v). …”
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Impact of COVID-19 on the Air Quality over China and India Using Long-term (2009–2020) Multi-satellite Data by Manish Soni, Sunita Verma, Hiren Jethava, Swagata Payra, Lok Lamsal, Priyanshu Gupta, Janhavi Singh

“…A noticeable difference in NO2 column burden is seen over SE (–35%), NE (–33%), NW (–13%) and SW (–5%) China. …”
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Standard reference values of the upper body posture in healthy young female adults in Germany: an observational study by Daniela Ohlendorf, Charlotte Doerry, Vanessa Fisch, Sebastian Schamberger, Gerhard Oremek, Hanns Ackermann, Johannes Schulze

“…Group differences were tested by the Wilcoxon Mann-Whitney U test.Results In normal posture, the spinal column was marginally twisted to the left, and the vertebrae were marginally rotated to the right. …”
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Thermographic Scan of the Thoracolumbar Area in Dogs with Acute Intervertebral Disc Extrusion (IVDE): A Retrospective Study by Cristian Zaha, Liliana Cărpinișan, Larisa Schuszler, Nistor Paula, Tudor Căsălean, Tiana Florea, Văduva Cristina, Bogdan Sicoe, Ciprian Rujescu, Roxana Dascălu

“…Background: several authors have documented variations in local temperature in both horses and dogs presenting acute intervertebral disc extrusion (IVDE) along the entire spinal column. However, none have demonstrated distinct temperature differences between healthy animals and those with IVDE. …”
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Anti-Inflammatory Effect Fraction of Bletilla striata and Its Protective Effect on LPS-Induced Acute Lung Injury by Chunchun Zhang, Dandan Ning, Jieli Pan, Cheng Chen, Chengxian Gao, Zhishan Ding, Fusheng Jiang, Meiya Li

“…EFBS was enriched by polyamide column chromatography and characterized by HPLC; an LPS-induced acute lung injury model was used to evaluate the anti-inflammatory activities of EFBS. …”
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Development and Validation of an Ion-Pair HPLC-UV Method for the Quantitation of Quinoline and Indoloquinoline Alkaloids in Herbal and Pharmaceutical Antimalarial Formulations by Samuel Oppong Bekoe, Emmanuel Orman, Samuel Awenteka Adjabui, Abena Amponsaa Brobbey, James Oppong-Kyekyeku, Kwabena Frimpong-Manso Opuni, Noble Kuntworbe, Samuel Asare-Nkansah

“…The separation/quantitation of the alkaloids (including quinine, quinidine, cinchonine, cinchonidine, dihydroquinine, dihydroquinidine, and cryptolepine) was achieved on a Zorbax SB-CN column (250 mm × 4.6 mm, 5 μm), with an isocratic elution system of methanol: trifluoroacetic acid (0.1%, v/v) (15 : 85, v/v) at 1.5 mL/min and 223 nm. …”
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ALMA Observations of Solar Spicules in a Polar Coronal Hole by T. S. Bastian, C. Alissandrakis, A. Nindos, M. Shimojo, S. M. White

“…The spicules observed at mm- λ are seen in absorption against coronal EUV emission, allowing us to estimate the column depth of neutral hydrogen. Spicular emission at mm- λ , due to thermal free–free radiation, allows us to estimate the electron number density as a function of height. …”
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Extraction, Enrichment, and Quantification of Main Antioxidant Aglycones of Flavonoids and Tannins from Melastoma Dodecandrum Lour.: Guided by UPLC-ESI-MS/MS by Yingpeng Tong, Yu Jiang, Xubo Chen, Xiaonan Li, Ping Wang, Ye Jin, Kejun Cheng

“…After the antioxidants were dynamically saturated by HPD 500, the column was eluted with different concentrations of ethanol (0, 25, 50, 75, and 100%, v/v). …”
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Use of Nanostructured Layered Double Hydroxides as Nanofilters in the Removal of Fe2+ and Ca2+ Ions from Oil Wells by Emmanuel K. Ephraim, Chinyere A. Anyama, Ayi A. Ayi, Jude C. Onwuka

“…The XRD pattern for Ca-Al-SO4 LDHs was quite different, showing the presence of low-angle peaks at 2θ = 11.68 and 14.72°. The results of the column adsorption studies showed that there was a significant removal of Ca2+ by all the compounds under investigation with an efficiency of 84–99%. …”
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High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride after In Vitro Transdermal Diffusion Studies by Sergio del Rio-Sancho, César E. Serna-Jiménez, M. Aracely Calatayud-Pascual, Cristina Balaguer-Fernández, Andrés Femenía-Font, Virginia Merino, Alicia López-Castellano

“…Separation was carried out with a 150 mm Kromasil C18 column at room temperature. The detection response, at 218 nm, was found to be linear in the concentration range from 0.5 to 50 μg/mL. …”
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