Transcription Factor FXR Activates DHRS9 to Inhibit the Cell Oxidative Phosphorylation and Suppress Colon Cancer Progression by Jinlai Zhao, Yigang Wang, Yang Wang, Jianchao Gao, Haichao Yang, Xiaotang Wu, Hua Li

“…The effects of FXR on the biological function of colon cancer cells were measured by Cell Counting Kit-8, colony formation, and transwell assays. The downstream target gene of FXR was predicted by bioinformatics analysis and found to be associated with cellular oxidative phosphorylation. …”
Get full text

In Vitro Evaluation of Proliferation and Migration Behaviour of Human Bone Marrow-Derived Mesenchymal Stem Cells in Presence of Platelet-Rich Plasma by Anh Thi Mai Nguyen, Ha Le Bao Tran, Thuy Anh Vu Pham

“…The proliferation of hBMSCs was examined by 2 methods: cell-number counting with the haemocytometer method and the colony-forming unit-fibroblast (CFU-F) assay. Cell migration was evaluated using the scratch wound healing (SWH) assay; after that, the recorded digital images were analysed by the Image-Analysis J 1.51j8 software to compare the cell-free areas between groups after 0, 24, and 48 hours. …”
Get full text

La datation des aqueducs lyonnais : un état des lieux by Aldo Borlenghi, Catherine Coquidé

“…The dating of the Roman aqueducts which served Colline de Fourvière, site of the Roman colony of Lugdunum, is a subject regularly addressed in the scientific literature. …”
Get full text

Determination of the physicochemical and microbiological quality of buffalo milk in the south of lake Maracaibo by Luis Álvarez, Alexis Zambrano, Datty Rosales-Zambrano

“…The amount of somatic cells was determined for each milk sample by direct microscopic somatic cell count (CCS) and bacterial colony count in a Petri dish (COVENIN 1126-1989, COVENIN 902-1987 and García, 2011). …”
Get full text

Differential Reponses of Hematopoietic Stem and Progenitor Cells to mTOR Inhibition by Aimin Yang, Xia Xiao, Mingfeng Zhao, Amanda C. LaRue, Bradley A. Schulte, Gavin Y. Wang

“…Here we report that dual inhibition of mTORC1/2 by AZD or its analogue (KU-63794) depletes mouse BM Lin−Sca-1+c-Kit+ cells in cultures via the induction of apoptotic cell death. Subsequent colony-forming unit (CFU) assays revealed that inhibition of mTORC1/2 suppresses the clonogenic function of hematopoietic progenitor cells (HPCs) in a dose-dependent manner. …”
Get full text

Gut microbiota derived DCA enhances FOLFOX efficacy via Ugt1a6b mediated enterohepatic circulation in colon cancer by Qian Fang, Xiaoying Hou, Limei Fan, Yufei Deng, Xiaoxuan Li, Hongyun Zhang, Haiping Wang, Zhengqi Fu, Binlian Sun, Xiji Shu, Hongzhi Du, Yuchen Liu

“…Further, based on the system screen of 15 bile acids on FOLFOX efficacy via MTT, colony formation and flow cytometry assay, Deoxycholic Acid (DCA) and Glycodeoxycholic Acid (GDCA) were annotated as potential modulators of FOLFOX efficacy. …”
Get full text

miR-504 Promoted Gastric Cancer Cell Proliferation and Inhibited Cell Apoptosis by Targeting RBM4 by Yi Zhang, Hongmei Yong, Jing Fu, Guangyi Gao, Huichang Shi, Xueyi Zhou, Mingsheng Fu

“…Cell proliferation and cell apoptosis were assessed by colony formation assay and flow cytometry, respectively. …”
Get full text

Autoimmune pulmonary alveolar proteinosis developed during treatment for systemic sclerosis: a case report by Tomoki Kozono, Kentaro Tanaka, Tomoko Yagi, Kazuto Kamikawaji, Masaki Watanabe, Azusa Iwanaga, Minako Hamada, Yoshifusa Koreeda, Hiromasa Inoue, Ikkou Higashimoto

“…Serum anti granulocyte–macrophage colony-stimulating factor (GM-CSF) antibodies were measured, and the result was positive, leading to the diagnosis of APAP. …”
Get full text

Experimental opisthorchiasis: a study of blood cells, hematopoiesis and startle reflex in laboratory animals by D. F. Avgustinovich, I. A. Orlovskaya, L. B. Toporkova, G. B. Vishnivetskaya, A. V. Katokhin, M. N. Lvova, E. V. Kashina, N. P. Bondar, N. A. Feofanova, V. A. Mordvinov

“…Blood changes in the response to infection in mice were less severe and were not accompanied by the changes in colony formation. Mouse acoustic startle reaction differed from hamster one too. …”
Get full text

Fermentation characteristics of lactofermented cucumbers using an isolated Lactiplantibacillus plantarum NCHU–FC1 strain by Hsuan-Min Wang, Shwu-Jene Tsai, Jin-Yuarn Lin

“…The pH value was quickly lower than 4.6 (aka high-acid foods), while total nitrite contents were below the standard limit using 1 × 107 colony forming unit (CFU)/mL Lpb. plantarum NCHU–FC1 at 4 °C for 5 days. …”
Get full text

Persea americana Glycolic Extract: In Vitro Study of Antimicrobial Activity against Candida albicans Biofilm and Cytotoxicity Evaluation by D. Jesus, J. R. Oliveira, F. E. Oliveira, K. C. Higa, J. C. Junqueira, A. O. C. Jorge, G. N. Back-Brito, L. D. Oliveira

“…After incubation (37°C/48 h), the values of colony forming units per milliliter (CFU/mL) were converted to log10 and analyzed (ANOVA and Tukey test, 5%). …”
Get full text

Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Health Care Workers in Tertiary and Regional Hospitals in Dar es Salam, Tanzania by Agricola Joachim, Sabrina J. Moyo, Lillian Nkinda, Mtebe Majigo, Sima Rugarabamu, Elizabeth G. Mkashabani, Elia J. Mmbaga, Naboth Mbembati, Said Aboud, Eligius F. Lyamuya

“…S. aureus was identified based on colonial morphology, Gram staining, catalase, coagulase, and DNase test results. …”
Get full text

Bacterial diversity determination using culture-dependent and culture-independent methods by M. Ghiasian, A. Akhavan Sepahy, M.A. Amoozegar, S. Saadatmand, M. Shavandi

“…A total of 122 isolates were obtained, 46 colonies were selected based on primarily morphological and physiological traits, and their 16S rRNA sequences were determined. …”
Get full text

Evaluation of the effect on antimicrobial properties, color, roughness and curing time of glass ionomer cement incorporated with silver and copper nanoparticles by Laís Aragão Lima, Wanessa Fernandes Matias Regis, Fábio Ruan Louzeiro Lima, Victor Teixeira Noronha, Amauri Jardim de Paula, Fernanda Freitas Sousa, Paulo Goberlânio Barros Silva, Rafael Melo Freire, Lidiany Karla Azevedo Rodrigues

“…The biofilms were then collected and plated on Mitis-Salivarius-Bacitracin (MSB) agar and Sabouraud dextrose agar for Streptococcus mutans and Candida albicans, respectively. Colony-forming unit (CFU) counts and dry weight were measured to assess the biofilm growth. …”
Get full text

Surface Modification by Combination of Dip-Pen Nanolithography and Soft Lithography for Reduction of Bacterial Adhesion by Santiago Arango-Santander, Alejandro Pelaez-Vargas, Sidónio C. Freitas, Claudia García

“…S. mutans adhesion was assessed by colony-forming units (CFUs), MTT viability assay, and scanning electron microscopy (SEM). …”
Get full text

p53-Dependent Senescence in Mesenchymal Stem Cells under Chronic Normoxia Is Potentiated by Low-Dose γ-Irradiation by Ines Höfig, Yashodhara Ingawale, Michael J. Atkinson, Heidi Hertlein, Peter J. Nelson, Michael Rosemann

“…Long-term observations revealed a decreasing ability of wildtype mMSCs to proliferate and form colonies under extended culture in normoxia. This was accompanied by increased senescence under normoxia but not associated with telomere shortening. …”
Get full text

A novel 4-(1,3,4-thiadiazole-2-ylthio)pyrimidine derivative inhibits cell proliferation by suppressing the MEK/ERK signaling pathway in colorectal cancer by Li Weiwei, Yang Zhifu, Ding Likun, Wang Ying, Zhao Xian, Chu Jian Jie, Ji Qing, Yao Minna, Wang Jingwen

“…Compound 4 also inhibited colony formation, migration, and invasion of HCT116 cells in a dose-dependent manner, as well as inducing cell apoptosis and arresting the cell cycle in the G2/M phase. …”
Get full text

Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway by Jiali Yang, Kangjian Zhang, Jing Wu, Juan Shi, Jing Xue, Jing Li, Juan Chen, Yongzhao Zhu, Jun Wei, Jinxi He, Xiaoming Liu

“…Results showed that the noncanonical Wnt signaling ligand, Wnt5a, could promote the proliferation, migration, invasion, and colony formation in A549 lung adenocarcinoma cells and cisplatin-resistant A549/DDP cells and increase the fraction of ALDH-positive cell in A549/DDP cells. …”
Get full text

First evidence of the effectiveness of a field application of RNAi technology in reducing infestation of the mite Varroa destructor in the western honey bee (Apis mellifera) by Francesca Bortolin, Emanuele Rigato, Sergio Perandin, Anna Granato, Laura Zulian, Caterina Millino, Beniamina Pacchioni, Franco Mutinelli, Giuseppe Fusco

“…Abstract Background The mite Varroa destructor is the most serious pest of the western honey bee (Apis mellifera) and a major factor in the global decline of colonies. Traditional control methods, such as chemical pesticides, although quick and temporarily effective, leave residues in hive products, harming bees and operators’ health, while promoting pathogen resistance and spread. …”
Get full text

Cold Plasma Activity Against Biofilm Formation of Prosthetic Joint Infection Pathogens by Christopher Spiegel, Débora C. Coraça-Huber, Michael Nogler, Rohit Arora, David Putzer

“…During the experiments, the duration and the distance of plasma application varied. Afterwards, colony-forming units were counted. Results: The results of this study showed that air and argon plasma could be considered for applications during surgeries at a 1 cm distance. …”
Get full text