Spatial Variability of Heavy Metals in Soils and Sediments of “La Zacatecana” Lagoon, Mexico by Sergio A. Covarrubias, Juan Armando Flores de la Torre, María Maldonado Vega, Francisco Javier Avelar González, Juan José Peña Cabriales

“…One particularly affected area in Mexico is the “La Zacatecana” Lagoon, in the municipality of Guadalupe, Zacatecas. From colonial times until the mid-nineteenth century, about 20 million tons of mine tailings were deposited at this site. …”
Get full text

Potential risks of bacterial plant pathogens from thawing permafrost in the Alaskan tundra by Dockyu Kim, Mincheol Kim, Sungho Woo, Sungjin Nam, Nu Ri Myeong, Eungbin Kim, Yung Mi Lee

“…Bacterial abundance, measured by colony-forming units and 16S rRNA gene copies, distinctly increased in the T and P layers after thawing. …”
Get full text

Human Suprapatellar Fat Pad-Derived Mesenchymal Stem Cells Induce Chondrogenesis and Cartilage Repair in a Model of Severe Osteoarthritis by Ignacio Muñoz-Criado, Jose Meseguer-Ripolles, Maravillas Mellado-López, Ana Alastrue-Agudo, Richard J Griffeth, Jerónimo Forteza-Vila, Ramón Cugat, Montserrat García, Victoria Moreno-Manzano

“…In addition, the suprapatellar SVF exhibited increased numbers of colony formation units and a higher population doubling in culture compared to the infrapatellar fraction. …”
Get full text

Anti-cancer activity of synthetic gefitinib-1,2,3-triazole derivatives against Hela cells via induction of apoptosis by Zhihong Hu, Xixi Hou, Yongjing Ren, Ziyuan Wu, Dong Yan, Hong Chen, Lan Wang

“…Moreover, compound c13 significantly inhibited the colony formation ability of Hela cells in a dose-dependent manner, accompanied by morphological changes in HeLa cells. …”
Get full text

Low Ethanol Concentrations Promote Endothelial Progenitor Cell Capacity and Reparative Function by Lars Brodowski, Bianca Schröder-Heurich, Berina Kipke, Cara Schmidt, Constantin S. von Kaisenberg, Frauke von Versen-Höynck

“…The aim of this study was to evaluate the effect of low dose ethanol on typical features of endothelial colony-forming cells (ECFCs), a proliferative subtype of EPCs. …”
Get full text

Cultivable Anaerobic Microbiota of Infected Root Canals by Takuichi Sato, Keiko Yamaki, Naoko Ishida, Kazuhiro Hashimoto, Yasuhisa Takeuchi, Megumi Shoji, Emika Sato, Junko Matsuyama, Hidetoshi Shimauchi, Nobuhiro Takahashi

“…Samples from infected root canals were collected, followed by anaerobic culture on CDC blood agar plates. After 7 days, colony forming units (CFU) were counted and isolated bacteria were identified by 16S rRNA gene sequencing. …”
Get full text

Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages by Elisabeth A. Diget, Kaja Zuwala, Randi K. Berg, Rune R. Laursen, Stine Søby, Lars Østergaard, Jesper Melchjorsen, Trine H. Mogensen

“…We found that elevated and persistent HIV-1 pWT/BaL replication could be obtained only in MDMs grown in RPMI containing macrophage colony-stimulating factor (M-CSF). Interestingly, this MDM type was also most responsive to toll-like receptor stimulation. …”
Get full text

MAGI2-AS3 hypermethylated in promoter region promotes migration and invasion of head and neck squamous cell carcinoma via miRNA-31-5p/AR axis by Kai Yue, Ting Zhang, Huanhuan Wang, Bo Wang, Yalin Mu, Hui Li

“…Molecular regulatory mechanism of MAGI2-AS3 in HNSCC is not yet mature.In this study, we analyzed the methylation level of MAGI2-AS3 promoter and its downstream miR-31-5p/AR axis by bioinformatics methods. qRT-PCR was used to detect the mRNA expression level of each gene, and western blot was used to detect the expression level of AR proteins in tissues and cells. CCK-8, colony formation, wound healing, and cellular invasion assays were used to detect the HNSCC cell proliferation, migration, and invasion. …”
Get full text

Modulation of Adipose-Derived Mesenchymal Stem/Stromal Cell Transcriptome by G-CSF Stimulation by Luz M. Avila-Portillo, Fabio Aristizabal, Angela Riveros, Martin C. Abba, Diego Correa

“…., priming) before their therapeutic use. Granulocyte-colony stimulating factor (G-CSF) is a cytokine that plays key roles in immune response and hematopoiesis modulation through direct effects on hematopoietic progenitors’ proliferation, survival, and mobilization. …”
Get full text

Antimicrobial Effects of Dental Luting Glass Ionomer Cements on Streptococcus mutans by Sina Klai, Markus Altenburger, Bettina Spitzmüller, Annette Anderson, Elmar Hellwig, Ali Al-Ahmad

“…Five different glass ionomer based luting cements were tested for their antimicrobial effects on Streptococcus mutans in two different experimental setups: (i) determination of colony-forming units (CFUs) in a plate-counting assay; (ii) live/dead staining (LDS) and fluorescence microscopy. …”
Get full text

‘Just because something works doesn’t mean it can’t be improved’: an ethnographic analysis of the health system in Black Panther’s Wakanda by Jennifer Jackson, Carla Ferreira, Samantha Perry, Carla Ginn, Melissa Scott, Elke Jaibeeh Barah, Zahra Upal, Krista Phelps, Carol Mei, Haneul Woo, Megan Tung, Farida Gadimova, Alexandra Ewanyshyn, Twyla Ens

“…We found that effective upstream approaches to health for all are embedded in anti-colonial philosophies. Wakandans embrace innovation, embedding biomedical engineering and continuous improvement into care settings. …”
Get full text

Quantitative Assessment of Optimal Bone Marrow Site for the Isolation of Porcine Mesenchymal Stem Cells by J. S. McDaniel, B. Antebi, M. Pilia, B. J. Hurtgen, S. Belenkiy, C. Necsoiu, L. C. Cancio, C. R. Rathbone, A. I. Batchinsky

“…Analyses of bone marrow aspirate (BMA) and concentrated bone marrow aspirate (cBMA) included bone marrow volume, platelet and nucleated cell yield, colony-forming unit fibroblast (CFU-F) number, flow cytometry, and assessment of differentiation potential. …”
Get full text

Sonication: A Valuable Technique for Diagnosis and Treatment of Periprosthetic Joint Infections by D. S. Evangelopoulos, I. P. Stathopoulos, G. P. Morassi, S. Koufos, A. Albarni, P. K. Karampinas, A. Stylianakis, S. Kohl, S. Pneumaticos, J. Vlamis

“…Sonication fluid cultures were examined for 10 days, and the number and identity of any colony morphology was recorded. In addition, periprosthetic tissue specimens (>5) were collected and cultured according to standard practice. …”
Get full text

Pulsed Electric Field Induces Significant Changes in the Metabolome of <i>Fusarium</i> Species and Decreases Their Viability and Toxigenicity by Adam Behner, Jana Palicova, Anna-Hirt Tobolkova, Nela Prusova, Milena Stranska

“…The viability of the <i>Fusarium</i> species was assessed by counting the colony-forming units, and changes in the mycotoxin content and metabolomic fingerprints were evaluated by using UHPLC-HRMS/MS instrumental analysis. …”
Get full text

Wharton’s Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential by Sun Jeong Kim, Sang Eon Park, Jang Bin Jeong, Shin Ji Oh, Alee Choi, Yun Hee Kim, Suk-joo Choi, Soo-young Oh, Gyu Ha Ryu, Hong Bae Jeon, Jong Wook Chang

“…Aurora kinase A (AURKA) and dedicator of cytokinesis 2 (DOCK2) were selected as the upregulated genes, and their effects on proliferation, migration, and colony formation of the WJ-MSCs were verified using small interfering RNA (siRNA) techniques. mRNA expression levels of both the genes were positively correlated with the proliferation capacity of WJ-MSCs. …”
Get full text

Cytokine-armed oncolytic herpes simplex viruses: a game-changer in cancer immunotherapy? by Howard L Kaufman, Hongbin Wang, Dipongkor Saha, Samuel D Rabkin, Mia Borlongan, Uyen Le, Hans J Nauwynck

“…Talimogene laherparepvec is an oncolytic herpes simplex virus type 1 (oHSV), approved for the treatment of recurrent melanoma, and the virus encodes the human cytokine, granulocyte-macrophage colony-stimulating factor (GM-CSF). A significant advantage of oncolytic viruses is the ability to deliver therapeutic payloads to the tumor site that can help drive antitumor immunity. …”
Get full text

Enfrentando lógicas coloniais na formação em Psicologia: Implicações à produção de cidadania by Gabriela da Cruz Miranda, Diângeli Strada de Almeida, Bárbara Magnani Rodrigues, Luciana Rodrigues

“…Por fim, apontamos para a necessidade de aprendermos com os ensinamentos de quem resiste e luta cotidianamente contra a violência de um projeto de mundo moderno colonial, oferecendo pistas para o exercício de uma psicologia comprometida com a transformação social. …”
Get full text

Assessment of the inhibitory effects of sodium nitrite, nisin, potassium sorbate, and sodium lactate on Staphylococcus aureus growth and staphylococcal enterotoxin A production in... by Lu Lin, Jie Yun Hu, Yi Wu, Min Chen, Jie Ou, Wei Ling Yan

“…Samples were analyzed every 3 h to count the S. aureus colonies and to detect SEA. The modified Gompertz model was used to describe S. aureus growth in the samples under various conditions, and the preservatives with a significant antimicrobial effect were selected. …”
Get full text

Drug-induced differential culturability in diverse strains of Mycobacterium tuberculosis by Valerie F. A. March, Nino Maghradze, Kakha Mchedlishvili, Teona Avaliani, Rusudan Aspindzelashvili, Zaza Avaliani, Maia Kipiani, Nestani Tukvadze, Levan Jugheli, Selim Bouaouina, Anna Doetsch, Galo A. Goig, Sebastien Gagneux, Sonia Borrell

“…Experimentally, there was a disparity between bacterial time to positivity (TTP) and colony forming units (CFUs) when Mtb was exposed to isoniazid (INH) and rifampicin (RIF) alone or in combination. …”
Get full text

Hematopoietic Stem and Progenitor Cell Expansion in Contact with Mesenchymal Stromal Cells in a Hanging Drop Model Uncovers Disadvantages of 3D Culture by Olga Schmal, Jan Seifert, Tilman E. Schäffer, Christina B. Walter, Wilhelm K. Aicher, Gerd Klein

“…Furthermore, expansion of primitive hematopoietic progenitors was more favored in 2D than in 3D, as analyzed in colony-forming assays. Conclusively, our data demonstrate that MSCs, when arranged with a spread (monolayer) shape, exhibit better HSPC supportive qualities than spheroid-forming MSCs. …”
Get full text