Targeted inhibition of the PTEN/PI3K/AKT pathway by YSV induces cell cycle arrest and apoptosis in oral squamous cell carcinoma by Lin Qiu, Qian Gao, Yiheng Liao, Xinxin Li, Cuiying Li

“…Methods A series of in vitro experiments were conducted to assess the impact of YSV on OSCC cell viability, colony formation, cell cycle, and apoptosis. RNA sequencing (RNA-seq), molecular docking, and western blotting were employed to investigate the molecular mechanisms. …”
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Potential Research Tool of Stem Cells from Human Exfoliated Deciduous Teeth: Lentiviral Bmi-1 Immortalization with EGFP Marker by Siqi Yao, Lingping Tan, Huan Chen, Xiaojun Huang, Wei Zhao, Yan Wang

“…Replicative lifespan determination and colony-forming efficiency assessment were using to assay proliferation capability. …”
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circSLC6A6 Sponges miR-497-5p to Promote Endometrial Cancer Progression via the PI4KB/Hedgehog Axis by Juan Hu, Xing Peng, Weina Du, Yichuan Huang, Chun Zhang, Xian Zhang

“…The luciferase reporter assay was performed to explore the connection between circSLC6A6 and miR-497-5p as well as the connection between miR-497-5p and PI4KB. The colony formation assay, EdU assay, wound healing assay, and transwell assay were performed to examine the proliferation, migration, and invasion of EC cells. …”
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Endoplasmic reticulum stress-related CLIP4 plays a procarcinogenic role in hepatocellular carcinoma: an integrated analysis by Anqi Wang, Sitong Yan, Weijia Jiang, Xiang Chen, Yuhan Huang, Xiangyu Zu, Xiao Du, Lulu Fan, Jiatao Liu, Guoping Sun

“…Public databases and miRNA-seq data were used to explore the TF-miRNA-CLIP4 regulatory network. CCK-8, colony formation, EdU staining, wound-healing, Transwell, western blotting and RT-qPCR were used to detect the effects of CLIP4 on the proliferation, migration and epithelial-mesenchymal transition (EMT) of HCC cells. …”
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CircCCT2/miR-146a-5p/IRAK1 axis promotes the development of head and neck squamous cell carcinoma by Long He, Lanruo Li, Liting Zhao, Xiaoya Guan, Yujia Guo, Qi Han, Huina Guo, Hongliang Liu, Chunming Zhang

“…CircCCT2 was characterized by Sanger sequencing, qRT-PCR, RNase R & Actinomycin D treatment, nucleoplasmic separation and FISH experiments. CCK-8 and colony formation assays were performed to determine cell proliferation, and Transwell assays were used to determine migration and invasion. …”
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Preparation of Sea-buckthorn Oil Microcapsule Composite Film and the Preservation Effect of Hand-grabbed Mutton by Bingbing ZHANG, Guishan LIU, Delang XIE, Haiyang MA, Yang LI, Fang MA, Xiaojun LI, Qingqing CAO, Xiaoxue LI, Qian ZHANG

“…The effects of composite membrane on color, pH, weight loss, TVB-N, total number of bacterial colonies and sensory indexes were analyzed to evaluate the fresh-keeping effect of hand-grabbed mutton. …”
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TOM40 as a prognostic oncogene for oral squamous cell carcinoma prognosis by Lifei Deng, Hong Ran, Dunhui Yang, Zhen Wang, Peng Zhao, Hengjie Huang, Yongjin Wu, Peng Zhang

“…The TCGA-OSCC cohort was divided into the TOM40 high and TOM40 low groups and the correlation between TOM40 expression level and the sensitivity to frequently used chemotherapeutic drugs was evaluated. CCK-8 and colony formation assays were applied to determine the cell growth. …”
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Tasquinimod suppresses tumor cell growth and bone resorption by targeting immunosuppressive myeloid cells and inhibiting c-MYC expression in multiple myeloma by Marie Törngren, Rong Fan, Eline Menu, Helena Eriksson, Karin Vanderkerken, Andrew Chantry, Hatice Satilmis, Niels Vandewalle, Emma Verheye, Philip Vlummens, Anke Maes, Catharina Muylaert, Elke De Bruyne, Holly Evans, Nathan De Beule, Dirk Hose, Ken Maes, Karine Breckpot, Kim De Veirman

“…The in vivo impact on immune cells (myeloid cell subsets, macrophages, dendritic cells), tumor load, survival and bone disease were elucidated using immunocompetent 5TMM models.Results Tasquinimod treatment significantly decreased myeloma cell proliferation and colony formation in vitro, associated with an inhibition of c-MYC and increased p27 expression. …”
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Cytokines and Water Distribution in Anorexia Nervosa by Hubertus Himmerich, Bethan Dalton, Olivia Patsalos, Ulrike Schmidt, Iain C. Campbell

“…Following blood collection, 27 cytokines and chemokines were quantified using multiplex ELISA-based technology: Eotaxin, Eotaxin-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon- (IFN-) γ, interleukin- (IL-) 1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, interferon γ-induced protein- (IP-) 10, macrophage inflammatory protein- (MIP-) 1α, MIP-1β, monocyte chemoattractant protein- (MCP-) 1, MCP-4, thymus and activation-regulated chemokine (TARC), TNF-α, and TNF-β. …”
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In vitro inhibition of Xanthomonas vasicola pv. musacearum, the causal agent of banana Xanthomonas Wilt, using medicinal plant extracts from North Kivu, Eastern Democratic Republic... by Franchement Mukeshambala, Angele Ibanda, Ludivine Lassois, Gakuru Semacumu, Dhed’a Djailo, Leon Nabahungu, Guy Blomme, Godefroid Monde

“…The bacterial samples were collected from infected field-grown banana plants in South Kivu and isolated on Yeast Extract Peptone Agar (YPGA). Pure Xvm colonies were used for identification via i) Polymerase Chain Reaction (PCR) with specific primers and, ii) greenhouse inoculation trials. …”
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USP39 phase separates into the nucleolus and drives lung adenocarcinoma progression by promoting GLI1 expression by Shaoxuan Cheng, Zhiyuan Qiu, Ziyi Zhang, Yuxuan Li, Yue Zhu, Yuxin Zhou, Yinghui Yang, Yaowen Zhang, Dian Yang, Yingqiu Zhang, Han Liu, Zhaoxia Dai, Shu-Lan Sun, Shuyan Liu

“…The effects of USP39 depletion on tumor progression were tested using doxycycline-inducible USP39 knockdown and rescue lung adenocarcinoma cells both in vitro and in vivo by performing MTT, colony formation, EdU staining, transwell and tumor xenograft model experiments. …”
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Evaluation of Yellow Fever Virus Infection in <i>Aedes aegypti</i> Mosquitoes from Pakistan with Distinct Knockdown Resistance Genotypes by Carlucio Rocha dos Santos, Caleb Guedes Miranda dos Santos, Dinair Couto-Lima, Bárbara Silva Souza, Rafi Ur Rahman, Marcos Dornelas Ribeiro, José Bento Pereira Lima, Ademir Jesus Martins

“…<i>aegypti</i> from a Pakistani colony were exposed to YFV strains (PR4408/2008 and ES504/2017) along with the Rockefeller strain. …”
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Domestication of different varieties in the cheese-making fungus Geotrichum candidum by Bennetot, Bastien, Vernadet, Jean-Philippe, Perkins, Vincent, Hautefeuille, Sophie, Rodríguez de la Vega, Ricardo C., O’Donnell, Samuel, Snirc, Alodie, Grondin, Cécile, Lessard, Marie-Hélène, Peron, Anne-Claire, Labrie, Steve, Landaud, Sophie, Giraud, Tatiana, Ropars, Jeanne

“…Another of the cheese populations seemed to represent a more advanced stage of domestication, with stronger phenotypic differentiation from the wild clade, harboring much lower genetic diversity, and phenotypes more typical of cheese fungi, with denser and fluffier colonies and a greater ability of excluding cheese spoiler fungi. …”
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Evidence that the domesticated fungus Leucoagaricus gongylophorus recycles its cytoplasmic contents as nutritional rewards to feed its leafcutter ant farmers by Caio Ambrosio Leal-Dutra, Lok Man Yuen, Bruno Augusto Maciel Guedes, Marta Contreras-Serrano, Pedro Elias Marques, Jonathan Zvi Shik

“…Abstract Leafcutter ants farm a fungal cultivar (Leucoagaricus gongylophorus) that converts inedible vegetation into food that sustains colonies with up to millions of workers. Analogous to edible fruits of crops domesticated by humans, L. gongylophorus has evolved specialized nutritional rewards—swollen hyphal cells called gongylidia that package metabolites and are consumed by ant farmers. …”
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Culturas en movimiento: migración y difusión-dilución de las creencias en México by Daniel Delaunay

“…En cuanto a los protestantes evangelistas, no se encontraran fundamentos demoeconómicos en su aptitud migratoria, por lo que ésta sí puede calificarse como cultural; su inclinación migratoria les sirve para difundir la iglesia evangelista, en particular las regiones de tradición más colonial que se resisten más a la dilución del catolicismo. …”
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Políticas de identidad y gestión sexual de los cuerpos: un análisis de los discursos legislativos acerca de la gestión estatal del conflicto trans en Chile by Dariela Milen Cristi Donoso

“…Lo que resulta clave para la reconfiguración de una ciudadanía excluyente, en cuanto se institucionalizan normas socio-sexuales que definirían las nociones emergentes de la figura de ciudadano/a, evidenciando que se enmarca en las fronteras del no-ser a toda corporalidad que no se manifieste bajo los márgenes de inteligibilidad que impone la lógica científica-colonial y la estructura político-administrativa del país.…”
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The XIAP inhibitor AZD5582 improves the treatment effect of microwave ablation on hepatocellular carcinoma by Wenhui Wang, Fuyuan Wu, Zhe Wu, Mengfan Zhang, Qiang Lu

“…We established an in vitro IMWA model by heating HCC cell lines and, at the same time, applied the XIAP inhibitor AZD5582 and verified the proliferation, migration, and pro-apoptotic ability of the XIAP inhibitor on tumor cells using CCK8, colony formation assay, cell scratch assay, and flow cytometry flow. …”
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The concept of natural genome reconstruction. Part 1. Basic provisions of the “natural genome reconstruction” concept. Changing the genome of hematopoietic stem cells using several... by L. A. Yakubov, O. S. Taranov, S. V. Sidorov, S. D. Nikonov, A. A. Ostanin, E. R. Chernykh, N. A. Kolchanov, S. S. Bogachev

“…Extracellular fragments delivered to the internal compartments of hematopoietic stem cells initiate terminal differentiation, colony formation, and proliferation of hematopoietic precursors. …”
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Generation and characterization of chicken monocyte-derived dendritic cells by Elie Ngantcha Tatchou, Elie Ngantcha Tatchou, Romane Milcamps, Romane Milcamps, Guillaume Oldenhove, Bénédicte Lambrecht, Fiona Ingrao

“…In chicken, DCs have mainly been derived from bone marrow cell cultures, with limited characterization from blood monocytes.MethodsThe present study takes advantage of newly available chicken immunological tools to further characterize chicken monocyte-derived dendritic cells (MoDCs), focusing on their phenotype, and functions, including antigen capture and T-cell stimulation, and response to live Newcastle disease virus (NDV) stimulation.ResultsAdherent chicken PBMCs were cultured with recombinant chicken granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4), for 5 days, leading to the upregulation of putative CD11c and MHCII, markers of DC differentiation. …”
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Construction and characterization of bioluminescent Salmonella Reading outbreak and non-outbreak strains by Abubakar Shitu Isah, Reshma Ramachandran, Anuraj Theradiyil Sukumaran, Aaron S. Kiess, Claudia D. Castañeda, Timothy Boltz, Kenneth S. Macklin, Hossam Abdelhamed, Li Zhang

“…Reading by electroporation. The resulting colonies were confirmed by visualizing bioluminescence using an in vivo imaging system and by testing their resistance to chloramphenicol. …”
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