Comparative Evaluation of Efficacy of Chlorhexidine and Herbal Mouthwash as a Preprocedural Rinse in Reducing Dental Aerosols: A Microbiological Study by Sangeeta Umesh Nayak, Anushka Kumari, Valliammai Rajendran, Vijendra Pal Singh, Ashwini Hegde, Keshava K. Pai

“…The microbial culture was analyzed. The colony-forming unit (CFU) counting in all three groups was assessed using one-way ANOVA test to compare among the groups (p value <0.001). …”
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Mebendazole Exerts Anticancer Activity in Ovarian Cancer Cell Lines via Novel Girdin-Mediated AKT/IKKα/β/NF-κB Signaling Axis by Rahul Gupta, Dipanjan Roy, Arijit Ghosh, Yasmin Begum, Dipanjan Ghosh, Snehasikta Swarnakar

“…Additionally, MBZ significantly impedes migration, spheroid invasion, colony formation, and stemness. In addition, it reduced actin polymerization and down-regulated CSC markers (e.g., CD24, CD44, EpCAM). …”
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NF-κB Inhibition after Cecal Ligation and Puncture Reduces Sepsis-Associated Lung Injury without Altering Bacterial Host Defense by Hui Li, Wei Han, Vasilly Polosukhin, Fiona E. Yull, Brahm H. Segal, Can-Mao Xie, Timothy S. Blackwell

“…Treatment with the NF-κB inhibitor did not affect the ability of cultured macrophages to phagocytose bacteria and did not alter bacterial colony counts in blood, lung tissue, or peritoneal fluid at 24 hours after CLP. …”
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Deformed wing virus coopts the host arginine kinase to enhance its fitness in honey bees (Apis mellifera) by Andrea Becchimanzi, Giovanna De Leva, Rosanna Mattossovich, Serena Camerini, Marialuisa Casella, Giovanni Jesu, Ilaria Di Lelio, Sabrina Di Giorgi, Joachim R. de Miranda, Anna Valenti, Silvia Gigliotti, Francesco Pennacchio

“…Abstract Background Deformed wing virus (DWV) is a major honey bee pathogen that is actively transmitted by the parasitic mite Varroa destructor and plays a primary role in Apis mellifera winter colony losses. Despite intense investigation on this pollinator, which has a unique environmental and economic importance, the mechanisms underlying the molecular interactions between DWV and honey bees are still poorly understood. …”
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METTL3-dependent DLG2 inhibits the malignant progression of cervical cancer by inactivating the Hippo/YAP signaling by Mei Pu, Xia Xiao, Shasha Lv, Daqing Ran, Qian Huang, Mingming Zhou, Qirong Lei, Lingshuang Kong, Qing Zhang

“…Methods Quantitative reverse transcription polymerase chain reaction and western blotting assays were employed to detect RNA and protein expression, respectively. Colony formation assay, 5-Ethynyl-2’-deoxyuridine assay, flow cytometry, and transwell assays were conducted for cell functional analysis. …”
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Reusable Tourniquets as Potential Transmitters of Infection: A Microbiological Analysis by Julia Szymczyk, Monika Kurpas, Bartosz Krasiński, Katarzyna Zorena, Wioletta Mędrzycka-Dąbrowska

“…Bacterial contamination on the surfaces of the tourniquets was measured using Columbia agar blood medium and expressed as colony-forming units (CFUs) per cm². Results: Significant bacterial loads were detected on reusable tourniquets, with contamination levels varying by location and duration of use. …”
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The Maternal Cytokine and Chemokine Profile of Naturally Conceived Gestations Is Mainly Preserved during In Vitro Fertilization and Egg Donation Pregnancies by Alicia Martínez-Varea, Begoña Pellicer, Vicente Serra, David Hervás-Marín, Alicia Martínez-Romero, José Bellver, Alfredo Perales-Marín, Antonio Pellicer

“…The main outcome measures were, firstly, to follow up plasma levels of interleukin (IL) 1beta, IL2, IL4, IL5, IL6, IL8, IL10, IL17, interferon gamma, tumor necrosis factor-alpha (TNFα), transforming growth factor-beta (TGFβ), regulated upon activation normal T-cell expressed and secreted (RANTES), stromal cell-derived factor 1 alpha (SDF1α), and decidual granulocyte-macrophage colony-stimulating factor (GM-CSF) during the three trimesters of pregnancy during the three trimesters of pregnancy; secondly, to evaluate if the cytokine and chemokine pattern of ED pregnant women differs from that of those with autologous oocytes and, thirdly, to assess if women with preeclampsia show different cytokine and chemokine profile throughout pregnancy versus women with uneventful pregnancies. …”
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Identification of a new alphacoronavirus (Coronaviridae: <i>Alphacoronavirus</i>) associated with the greater horseshoe bat (<i>Rhinolophus ferrumequinum</i>) in the south of Europ... by Sergey V. Lenshin, Tatyana V. Vishnevskaya, Alexey V. Romashin, Yulia I. Bulycheva, Oleg I. Vyshemirsky, Sophya A. Solovyeva, Asya K. Gitelman, Alexey S. Pazilin, Dmitry K. Lvov, Ben Hu, Zheng-Li Shi, Sergey V. Alkhovsky

“…Infection rates in a single R. ferrumequinum colony during autumn 2021 reached 59.2% and 70.5% for Kudep and Khosta-1, respectively. …”
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Intra-Amnionic Threonine Administered to Chicken Embryos Reduces Salmonella Enteritidis Cecal Counts and Improves Posthatch Intestinal Development by Alexandre L. de B. Moreira Filho, Celso J. B. Oliveira, Oliveiro C. Freitas Neto, Candice M. C. G. de Leon, Mauro M. S. Saraiva, Maria F. S. Andrade, Bryan White, Patrícia E. N. Givisiez

“…At 2 days of age, half of the birds from each in ovo treatment were given either 0.5 mL of nutrient broth (sham-inoculated) or nalidixic acid-resistant Salmonella Enteritidis (SE NalR) in nutrient broth (8.3 × 107 colony forming units (CFU) SE NalR/mL). The birds were distributed using a completely randomized design with four treatments after the Salmonella challenge: no in ovo Thr supplementation and sham-inoculated in the posthatch challenge (NT-SHAM), in ovo Thr supplementation and sham-inoculated (T-SHAM), no in ovo Thr supplementation and SE NalR-challenged (NT-SE), and in ovo Thr supplementation and SE NalR-challenged (T-SE). …”
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Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of Inflammation by Felipe Fornias Sperandio, Gisele Pesquero Fernandes, Ana Carolina Silvério Cerqueira Mendes, Giulia Maria de Alencar Castro Bani, Vera Lucia Garcia Calich, Eva Burger

“…We also assessed the fungal colony forming units and the levels of soluble inflammatory mediators (LTB4, KC, IFN-γ, GM-CSF, and IL-10) inside subcutaneous air-pouches to compare the efficiency of the PMN present at this site in relation to the two main neutrophil functions: initial lysis of the invading pathogen and modulation of the acquired immune response. …”
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Coexistence of blaMIR-1 and blaNDM-1 resistance genes with a novel ST type in Enterobacter roggenkampii from a stool sample: A genome sequencing study by Qin Wang, Sayyed Salman, Ye Luo, XiaoMei Tong

“…Cultures were then spread on MacConkey agar containing meropenem and incubated for 18–24 h to select carbapenem-resistant Enterobacteriaceae. Individual colonies were isolated, and bacterial species were identified using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and 16S rRNA gene sequencing. …”
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Transcriptome Analysis Revealed the Molecular Mechanism of Acetic Acid Increasing <i>Monascus</i> Pigment Production in <i>Monascus ruber</i> CICC41233 by Yan Wang, Weiwei Wu, Xiaoshu Wu, Weiyu Li, Jingjing Cui, Chuannan Long

“…Compared with no acetic acid or HCl addition, the diameter of <i>M. ruber</i> CICC41233 colonies increased significantly under acetic acid conditions. …”
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Evaluation of 16s Long Read Metabarcoding for Characterizing the Microbiome and Salmonella Contamination of Retail Poultry Meat by Zachary Tobar, Katie Y. Lee, Megan E. Gaa, Bryshal P. Moore, Xunde Li, Maurice E. Pitesky

“…Results indicate the 16s metabarcoding assay evaluated here could not be reliably used for the detection of Salmonella in adulterated retail meat samples as the LOD observed, 4.70 log colony forming units (CFU)/ml, is above the expected concentration of Salmonella in retail poultry meat samples which previous studies have shown range from under 1 to 2 log CFU/ml. …”
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Polysaccharide Fraction Isolated from <i>Saccharina japonica</i> Exhibits Anti-Cancer Effects Through Immunostimulating Activities by Min Seung Park, Seung-U Son, Tae Eun Kim, Se Hyun Shim, Bong-Keun Jang, Sunyoung Park, Kwang-Soon Shin

“…</i> administration of SJP at a dose of 50 mg/kg reduced tumor colonies by 84% and 40%, respectively, compared to the control. …”
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ENO1 promotes PDAC progression by inhibiting CD8+ T cell infiltration through upregulating PD-L1 expression via HIF-1α signaling by Aziguli Tulamaiti, Shu-Yu Xiao, Yan Yang, Musitaba Mutailifu, Xia-Qing Li, Shi-Qi Yin, Hong-Tai Ma, Hong-Fei Yao, Lin-Li Yao, Li-Peng Hu, Jun Li, Shu-Heng Jiang, Zhi-Gang Zhang, Yan-Miao Huo, Dong-Xue Li, Xue-Li Zhang

“…In vitro, Eno1 knockdown resulted in reduced PDAC cell proliferation and colony formation, along with enhanced apoptosis in PDAC cells. …”
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Isolation of Helicobacter pylori from Gastric Biopsy of Dyspeptic Patients in Ghana and In Vitro Preliminary Assessment of the Effect of Dissotis rotundifolia Extract on Its Growth by Michael Buenor Adinortey, Charles Ansah, Cynthia Ayefoumi Adinortey, Ansumana Sandy Bockarie, Martin Tangnaa Morna, Damian H. Amewowor

“…Pure cultures of H. pylori in 2,3,5-triphenyltetrazolium chloride (TTC) dye incorporated medium were seen as sparkling colonies. Isolates, identified as H. pylori, were gram-negative and urease, catalase, and oxidase positive and showed characteristic morphology as spiral-shaped bacteria under the microscope. …”
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Hydrocarbon contaminated water remediation using a locally constructed multi-stage bioreactor incorporated with media filtration by S. Antwi-Akomea, B. Fei-Baffoe, E.J.D. Belford, M. Borigu

“…Microbial load reduction ranged from 3.57±0.11E+20 to 7.45±0.26E+20 Colony forming unit/mL, total dissolved solids reduction from 30.00±5.66 to 131.00±0.00 mg/L and turbidity reduction from 39.00±1.41 to 123.50±0.71 nephelometric turbidity units. …”
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Isolation and characterization of mercuric reductase by newly isolated halophilic bacterium, Bacillus firmus MN8 by M. Noroozi, M.A. Amoozegar, A.A. Pourbabaei, N.S. Naghavi, Z. Nourmohammadi

“…To examine the tolerance of purified colonies to mercury, agar dilution method was administered. …”
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Distribution and clinicopathological characteristics of G-CSF expression in tumor cells and stromal cells in upper tract urothelial carcinoma by Go Kobayashi, Yohei Sekino, Hikaru Nakahara, Kohei Kobatake, Keisuke Goto, Tetsutaro Hayashi, Kazuhiro Sentani, Nobuyuki Hinata

“…Methods To clarify the clinicopathological significance of granulocyte-colony stimulating factor (G-CSF) in UTUC, we analyzed the expression and distribution of G-CSF in 112 upper tract urothelial carcinoma (UTUC) samples with immunohistochemistry. …”
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Two novel entomopathogenic fungal species of Lecanicillium isolated from soil in China by Tongyi Liu, Wei Chen, Yanhong Tang, Fen Liu, Liyan Yao, Qiongbo Hu, Ke Zhang, Qunfang Weng

“…They differ from known closely related species primarily in sporulation structure, conidia and colony morphological characteristics, and sequence variations in the LSU, SSU, ITS, and TEF gene regions of the ribosomal DNA. …”
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