Promoting COVID-19 Vaccine Acceptance through Community Engagement: An Ethnographic Study in Ghana by Matilda Aberese-Ako, Wisdom Ebelin, Phidelia Theresa Doegah, Mawulom Kuatewo, Lebene Kpodo, Atsu Godsway Kpordorlor, Robert Kaba Alhassan, Atubiga Alobit Baba, Senanu Djokoto, Evelyn K. Ansah

“…Audio interviews were transcribed and uploaded to Nvivo 12 to support triangulation, coding, and thematic analysis. Ethical approval was obtained from the requisite authority, and all COVID-19 restrictions were observed. …”
Get full text

An assessment of compliance with proposed regulations to restrict on-package marketing of packaged foods to improve nutrition in South Africa by Safura Abdool Karim, Tamryn Frank, Alice S. Khan, Morongoa G. Tlhako, Sikhumbule K. Joni, Elizabeth C. Swart

“…Food packages and their nutritional information were coded and analyzed to determine whether the product would be required to carry a WL and whether the package included regulated marketing. …”
Get full text

mRNA-lncRNA gene expression signature in HPV-associated neoplasia and cervical cancer by E. D. Kulaeva, E. S. Muzlaeva, E. V. Mashkina

“…Along with the protein-coding genes, long noncoding RNAs (lncRNAs) play an important role in the development of malignant cell transformation. …”
Get full text

Emotional impact on healthcare providers involved in medical assistance in dying (MAiD): a systematic review and qualitative meta-synthesis by Alexander Simpson, Alireza Bagheri, Saumil Yogendra Dholakia

“…Data from 35 studies (393 physicians, 169 nurses, 53 social workers, 22 allied healthcare professionals) employing diverse qualitative research methodologies from five countries were coded and analysed. The thematic meta-synthesis showed three descriptive emotional themes: (1) polarised emotions including moral distress (n=153), (2) reflective emotions with MAiD as a ‘sense-making process’ (n=251), and (3) professional value-driven emotions (n=352).Discussion This research attempts to answer the question, ‘what it means at an emotional level’, for a MAiD practitioner. …”
Get full text

Patient’s Information Toward Some Modifiable Risk Factors of Ischemic Heart Disease by Bayan Omar Sharif, Aras Hamad Rasul, Osman Ibrahim Mahmud, Farman Nuri Abdulla

“…Reliability of the instrument was determined through the use of internal consistency reliability (split half) approach which was estimated as r = (0.83) the data were collected through the use of interview technique (face to face approach), the computer files is used to organizing and coding it. The data analyzed by Statistical approaches which includes: descriptive and inferential statistical and chi- square, data analysis (SPSS version 25). …”
Get full text

Postoperative Complications After Operative Treatment of Open Versus Closed Distal Radius Fractures: A Propensity Score Matched Analysis by R. Cole Schmidt, MD, Conor N. O’Neill, MD, Zakkary J. Walterscheid, MD, James R. Satalich, MD, Jonathan Isaacs, MD

“…Methods: Patients in the American College of Surgeons National Surgical Quality Improvement Program database who underwent open reduction and internal fixation of a distal radius fracture between 2008 and 2018 were identified using Current Procedural Terminology codes and stratified into open (OF) and closed fractures (CF). …”
Get full text

Development and validation of highly sensitive multiplex real-time RT-PCR assay for detection of classical swine fever virus genome by A. S. Sadchikova, A. S. Igolkin, R. S. Chernyshev, A. A. Kozlov, I. S. Kolbin, A. V. Sprygin, D. A. Biryuchenkov, I. A. Chvala, A. Mazloum

“…For comparative analysis of the developed as say sensitivity, swabs, samples of organs and tissues collected from pigs experimentally infected with an epizootic strain of the classical swine fever virus (spleen, kidney, liver, blood, lymph nodes, rectal and oral smears), animal-contaminated feed and virus-containing material with known virus titres were also tested in parallel with coded test systems No. x1 andx2. The developed assay was shown to have 100% diagnostic sensitivity and detection limit of 0,23 lgCCID50/cm3. …”
Get full text

Transfer of optogenetic vectors into the brain of neonatal animals to study neuron functions during subsequent periods of development by D. A. Lanshakov, U. S. Drozd, T. A. Zapara, N. N. Dygalo

“…In this analysis, 3-day-old rat pups received intracerebroventricular injections of optovector (pAAV-CAMKIIa-ChR2h134-YFP), coding for a photo channel, which activates neurons, and the yellow fluorescent marker protein under the CAMKIIa promoter specific for glutamatergic neurons under cold anesthesia. …”
Get full text

Linking altered neuronal and synaptic properties to nicotinic receptor Alpha5 subunit gene dysfunction: a translational investigation in rat mPFC and human cortical layer 6 by Danqing Yang, Guanxiao Qi, Daniel Delev, Uwe Maskos, Dirk Feldmeyer

“…We performed single cell-electrophysiology combined with morphological reconstructions on layer 6 (L6) excitatory neurons in the medial PFC (mPFC) of wild type (WT) rats, rats carrying the human coding polymorphism rs16969968 in Chrna5 and α5 knockout (KO) rats. …”
Get full text

Evaluating the role of selection in the evolution of mitochondrial genomes of aboriginal peoples of Siberia by B. A. Malyarchuk, M. V. Derenko

“…Studies of the nature of mitochondrial DNA (mtDNA) variability in human populations have shown that protein­coding genes are under negative (purifying) selection, since their mutation spectra are characterized by a pro­ nounced predominance of synonymous substitutions over non­synonymous ones (Ka/Ks < 1). …”
Get full text

Advancing Building and Construction Higher Education: The Online Real-Time Block Model’s Contributions to Professional Skills, Gender Equity, and Industry Preparedness by Nima Izadyar, Le Li, Shuo Chen, Darryl O’Brien

“…Building surveying as a critical field in construction is selected as the case study since professional surveyors must stay current with rapidly evolving building codes, regulations, and sustainability practices. …”
Get full text

Evaluation of three remineralizing agents on surface roughness of enamel. by Vian O. Majeed, Hemn M. Sarmamy, Rebin A. Mohammedamin

“…Each tooth was soaked in demineralizing solution at 37oC for14 days to produce a white spot lesion. Then samples were coded from 1 to 40 for identification purposes and middle of buccal surface were subjected to profilometric analysis for obtaining enamel surface roughness parameters prior to any treatment (1st roughness recording). …”
Get full text

Virtual consultation in kidney care: a mixed-methods study on a model for safe and effective integration into routine clinical care by Aminu K Bello, Stephanie Thompson, Deenaz Zaidi, Scott Klarenbach, Feng Ye, Terry J Smith, Mark Courtney

“…This study evaluated the integration of virtual outpatient consultation safely and effectively into the kidney care programme in Alberta.Study design We leveraged a mixed-methods approach to collate data about clinicians’ experiences and opinions, forming the basis for the qualitative part of the study.Data extraction Data were collected through surveys, interviews and focus groups of nephrologists and home dialysis nurses.Analytical approach Focus group/interview transcripts for nephrologists and nurses were used to generate initial codebooks, which were iteratively refined throughout the analysis. Codes were categorised and analysed thematically, and data collected from nephrologists and nurses were analysed separately.Results The findings demonstrated that clinicians support the use of routine virtual care. …”
Get full text

Design and expression of a chimeric recombinant antigen (SsIR-Ss1a) for the serodiagnosis of human strongyloidiasis: Evaluation of performance, sensitivity, and specificity. by Mostafa Omidian, Zohreh Mostafavi-Pour, Marzieh Asadi, Meysam Sharifdini, Navid Nezafat, Ali Pouryousef, Amir Savardashtaki, Mortaza Taheri-Anganeh, Fattaneh Mikaeili, Bahador Sarkari

“…<h4>Methodology/principal findings</h4>The coding sequences for SsIR and Ss1a were selected from GenBank and were gene-optimized. …”
Get full text

How do electronic risk assessment tools affect the communication and understanding of diagnostic uncertainty in the primary care consultation? A systematic review and thematic synt... by Sarah Gerard Dean, Joshua Feyi-Waboso, Mark Tarrant, Raff Calitri, Elizabeth Shephard, Alex Burns, Brian Donnelly

“…Data extraction was via line by line coding. A thematic synthesis was performed.Setting Primary care.Results eRATs included differential diagnosis suggestion tools, tools which produce a future risk of disease development or recurrence or calculate a risk of current undiagnosed disease. …”
Get full text

Prevalence of Obstetric Danger Signs during Pregnancy and Associated Factors among Mothers in Shashemene Rural District, South Ethiopia by Nega Terefe, Aderajew Nigussie, Afework Tadele

“…A pretested interviewer-administered questionnaire was utilized. Data were cleaned, coded, and entered into Epi data manager version 4.1 and then exported to SPSS version 20. …”
Get full text

Evaluation of Intestinal Parasite Infection in Low and High Coverage of Graduated Households, Northwest Ethiopia: A Comparative-Based Crosssectional Study by Desalegn Andargie, Yalewayker Tegegne, Ligabaw Worku

“…Five grams of stool specimen was collected, and samples were processed using a direct wet mount and Kato Katz technique. Data were coded, entered, and cleaned using statistical package for social science (SPSS) version 20. …”
Get full text

Screening and Whole-Genome Sequencing Analysis of Fructofuranose-Metabolizing Lactiplantibacillus plantarum 19M03 by SUN Daqing, QI He, DI Ziqing, GE Xianglin, HONG Qingping, DU Xinrui, YAO Yuxi, LI Hongfei

“…The sequencing results showed that the genome of strain 19M03 contained one chromosome and nine plasmids, with a total length of 3 246 316 bp, GC content of 44.53%, 3 084 coding genes, 66 tRNAs and 16 rRNAs. The phylogenetic relationship analysis showed that strain 19M03 belonged to Lactiplantibacillus plantarum. …”
Get full text

Tetracycline and chloramphenicol exposure induce decreased susceptibility to tigecycline and genetic alterations in AcrAB-TolC efflux pump regulators in Escherichia coli and Klebsi... by Nian Anwar Nasralddin, Mehri Haeili, Sasan Karimzadeh, Fatemeh Alsahlani

“…The genetic alterations of genes coding for major AcrAB regulators including acrR (repressor of acrAB), ramR (repressor of ramA), soxR (repressor of soxS) in K. pneumoniae and lon (proteolytic degradation of MarA), marR (repressor of marA), acrR and soxR in E. coli were investigated. …”
Get full text

Assessing patient experiences with a Virtual Triage and Assessment Centre (VTAC): a mixed-methods study using an online survey and semi-structured interviews in Renfrew County, Ont... by Antoine St-Amant, Cayden Peixoto, Dez Bair-Patel, Martha Heideman, Kayla Menkhorst, Jonathan Fitzsimon

“…Survey data were analyzed through descriptive statistics, chi-squared tests, and a multivariate binary logistic regression, while semi-structured interviews were coded and analyzed using reflexive thematic analysis. …”
Get full text