Chromatin Modifying Agents in the In Vitro Production of Bovine Embryos by Fabio Morato Monteiro, Clara Slade Oliveira, Letícia Zoccolaro Oliveira, Naiara Zoccal Saraiva, Maria Eugênia Zerlotti Mercadante, Flavia Lombardi Lopes, Daniel Robert Arnold, Joaquim Mansano Garcia

“…The low efficiency observed in cloning by nuclear transfer is related to an aberrant gene expression following errors in epigenetic reprogramming. …”
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Production of β-lactoglobulin (BLG) gene knock- out blastocyst stage embryos of Indian water buffalo using CRISPR and SCNT technology by Aseem Tara, Priyanka Singh, Devika Gautam, Gaurav Tripathi, Shreya Malhotra, Sacchinandan De, Manoj K. Singh, Naresh L. Selokar

“…Among three sgRNAs, the most efficient sgRNA was used to generate the clonal population of edited cells. Several single-cell clones were established and screened using the TA cloning (also known as rapid cloning or T cloning) and Sanger sequencing methods. …”
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Modulating gene expression as a strategy to investigate thyroid cancer biology by Diego Claro de Mello, Joice Moraes Menezes, Antonio Tarelo Freitas de Oliveira, Marcella Maringolo Cristovão, Edna Teruko Kimura, Cesar Seigi Fuziwara

“…This strategy has evolved methodologically due to advances in cloning approaches, modeling/algorithms in short hairpin RNA (shRNA) design for knockdown efficiency, and biochemical modifications in RNA synthesis, among other developments. …”
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Development of a broad-spectrum epitope-based vaccine against Streptococcus pneumoniae. by Md Nahian, Muhammad Shahab, Md Rasel Khan, Shopnil Akash, Tanjina Akhtar Banu, Murshed Hasan Sarkar, Barna Goswami, Sanjana Fatema Chowdhury, Mohammad Ariful Islam, Ahmed Abu Rus'd, Shamima Begum, Ahashan Habib, Aftab Ali Shaikh, Jonas Ivan Nobre Oliveira, Shahina Akter

“…GenScript server confirmed the high efficiency of cloning for the construct and in-silico cloning into the pET28a (+) vector using SnapGene, demonstrating successful translation of the epitope region. …”
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Immunoinformatic strategy for developing multi-epitope subunit vaccine against Helicobacter pylori. by Md Nahian, Md Rasel Khan, Fabiha Rahman, Hossain Mohammed Reza, Imren Bayil, Tanjum Ahmed Nodee, Tabassum Basher, Mostafizur Rahaman Sany, Rabeya Najnin Munmun, S M Ariful Habib, Lincon Mazumder, Mrityunjoy Acharjee

“…The proposed vaccine candidates were evaluated based on population coverage, molecular docking, immune simulations, codon adaptation, secondary mRNA structure, and in silico cloning. The vaccine candidates exhibited antigenic scores of 1.19 and 1.01, with 93.5% and 90.4% of the most rama-favored regions, respectively. …”
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Maintien du potentiel adaptatif chez les plantes domestiquées à propagation clonale by Doyle McKey, Marianne Elias, Benoît Pujol, Anne Duputié, Marc Delêtre, Delphine Renard

“…It is propagated by farmers by stem cuttings : a small section of stem is cut from a mature plant and directly planted in the ground. This amounts to cloning. Cloning is the only efficient way to “capture” and multiply initially rare favourable phenotypes in habitually outcrossed plants such as manioc. …”
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Immunoinformatics Approach to Design a Chimeric CD70-Peptide Vaccine against Renal Cell Carcinoma by Haideh Namdari, Farhad Rezaei, Fatemeh Heidarnejad, Mohammad Yaghoubzad-Maleki, Maryam Karamigolbaghi

“…In silico codon optimization and cloning of the vaccine nucleic acid sequence were accomplished using the pET28a plasmid. …”
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A security authentication scheme in RFID system for smart park by Rongrong LI, Jiantao KOU, Gang DONG, Liangrui TANG

“…The new protocol could effectively resist attacks of re-transmission，tracking，deception，cloning and de-synchronizationand，and it had high security and practicability.…”
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A systematic screening assay identifies efficient small guide RNAs for CRISPR activation by Elin Arvidsson, Diana Duarte Lobo, Diana Duarte Lobo, Ermelinda Sabarese, Fabio Duarte, Rui Jorge Nobre, Rui Jorge Nobre, Rui Jorge Nobre, Luis Quintino, Cecilia Lundberg

“…In addition, we provide a tailored Golden Gate cloning workflow for streamlined incorporation of selected sgRNA candidates into lentiviral (LVs) or adeno-associated viral vectors (AAVs). …”
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Generation of stably expressing IRF5 spliced isoform in Jurkat cells by Ashwinder Kaur, Chee-Mun Fang

“… Lentiviral transduction enables the generation of gain-of-function of a targeted gene in mammalian cells. Single-cell cloning through limiting dilution can establish a population of cells with homogenous transgene expression for exploring protein function. …”
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Tropical and Subtropical Fruit Propagation by Jeff Wasielewski, Carlos F. Balerdi

“…While sexual propagation (by seed) results in plants that are not genetically the same as the mother plant, asexual propagation (cuttings, division, air-layers, and grafting) creates offspring that are clones of the mother plant. Cloning fruit trees is important because it allows different cultivars to be preserved over time. …”
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Tropical and Subtropical Fruit Propagation by Jeff Wasielewski, Carlos F. Balerdi

“…While sexual propagation (by seed) results in plants that are not genetically the same as the mother plant, asexual propagation (cuttings, division, air-layers, and grafting) creates offspring that are clones of the mother plant. Cloning fruit trees is important because it allows different cultivars to be preserved over time. …”
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Quantum Internet: key technologies and future prospect by Zishan LIU, Junsen LAI, Wenyu ZHAO

“…The quantum Internet integrates quantum computing, quantum measurement and communication, which can be described as the future goal of the evolution of quantum information technology.However, due to the restrictions of the laws of quantum mechanics, such as quantum teleportation, quantum entanglement, quantum measurement and non-cloning, which all pose new challenges to the quantum networking design.The basic concepts and development paths of the quantum Internet were firstly introduced, and the implementation differences between the classical network and the quantum network were explained.Considering the difference between quantum communication and the traditional communication, the key technologies for realizing the quantum Internet including the quantum physical devices, networking protocol, quantum decoherence and quantum relay were introduced, and finally the prospect and suggestions for developing quantum Internet was presented.…”
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Designing a broad-spectrum multi-epitope subunit vaccine against leptospirosis using immunoinformatics and structural approaches by Guneswar Sethi, Guneswar Sethi, Young Kyu Kim, Su-Cheol Han, Jeong Ho Hwang, Jeong Ho Hwang

“…Immune simulations and in silico cloning were also conducted to evaluate the vaccine’s immune response and expression potential.ResultsThe MESV demonstrated high antigenicity, immunogenicity, non-allergenicity, and conservation across different Leptospira strains. …”
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Human Fetal Liver: An In Vitro Model of Erythropoiesis by Guillaume Pourcher, Christelle Mazurier, Yé Yong King, Marie-Catherine Giarratana, Ladan Kobari, Daniela Boehm, Luc Douay, Hélène Lapillonne

“…In this in vitro model, maturation that is enucleation reaches a lower level compared to adult sources as observed for embryonic or iP, but, interestingly, they (i) displayed a dramatic in vitro expansion (100-fold more when compared to CB CD34+) and (ii) 100% cloning efficiency in hematopoietic progenitor assays after 3 days of erythroid induction, as compared to 10–15% cloning efficiency for adult CD34+ cells. …”
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Synthesis of symmetric paths of arbiter physically unclonable function on FPGA by A. A. Ivaniuk

“…Circuit design solutions in physical cryptography are called digital physically unclonable functions (PUFs), which to be implemented ensure the uniqueness, non-reproducibility (non-cloning) of the protected digital device. In addition, PUFs should be efficient as hardware resources. …”
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