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Suggested Topics within your search.
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Chromatin Modifying Agents in the In Vitro Production of Bovine Embryos
Published 2011-01-01“…The low efficiency observed in cloning by nuclear transfer is related to an aberrant gene expression following errors in epigenetic reprogramming. …”
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Production of β-lactoglobulin (BLG) gene knock- out blastocyst stage embryos of Indian water buffalo using CRISPR and SCNT technology
Published 2023-11-01“…Among three sgRNAs, the most efficient sgRNA was used to generate the clonal population of edited cells. Several single-cell clones were established and screened using the TA cloning (also known as rapid cloning or T cloning) and Sanger sequencing methods. …”
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125
Modulating gene expression as a strategy to investigate thyroid cancer biology
Published 2025-01-01“…This strategy has evolved methodologically due to advances in cloning approaches, modeling/algorithms in short hairpin RNA (shRNA) design for knockdown efficiency, and biochemical modifications in RNA synthesis, among other developments. …”
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126
Development of a broad-spectrum epitope-based vaccine against Streptococcus pneumoniae.
Published 2025-01-01“…GenScript server confirmed the high efficiency of cloning for the construct and in-silico cloning into the pET28a (+) vector using SnapGene, demonstrating successful translation of the epitope region. …”
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127
Immunoinformatic strategy for developing multi-epitope subunit vaccine against Helicobacter pylori.
Published 2025-01-01“…The proposed vaccine candidates were evaluated based on population coverage, molecular docking, immune simulations, codon adaptation, secondary mRNA structure, and in silico cloning. The vaccine candidates exhibited antigenic scores of 1.19 and 1.01, with 93.5% and 90.4% of the most rama-favored regions, respectively. …”
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128
Maintien du potentiel adaptatif chez les plantes domestiquées à propagation clonale
Published 2012-11-01“…It is propagated by farmers by stem cuttings : a small section of stem is cut from a mature plant and directly planted in the ground. This amounts to cloning. Cloning is the only efficient way to “capture” and multiply initially rare favourable phenotypes in habitually outcrossed plants such as manioc. …”
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129
Immunoinformatics Approach to Design a Chimeric CD70-Peptide Vaccine against Renal Cell Carcinoma
Published 2024-01-01“…In silico codon optimization and cloning of the vaccine nucleic acid sequence were accomplished using the pET28a plasmid. …”
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130
A systematic screening assay identifies efficient small guide RNAs for CRISPR activation
Published 2025-01-01“…In addition, we provide a tailored Golden Gate cloning workflow for streamlined incorporation of selected sgRNA candidates into lentiviral (LVs) or adeno-associated viral vectors (AAVs). …”
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131
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology /
Published 2018Table of Contents: “…Biochemical and molecular biological methods in life sciences studies -- Basic principles -- Cell culture techniques -- Recombinant DNA techniques and molecular cloning -- Preparative protein biochemistry -- Electrophoretic techniques -- Immunochemical techniques -- Flow cytometry -- Radioisotope techniques -- Principles of clinical biochemistry -- Microscopy -- Centrifugation and ultracentrifugation -- Spectroscopic techniques -- Basic techniques probing molecular structure and interactions -- Mass spectrometric techniques -- Fundamentals of bioinformatics -- Fundamentals of chemoinformatics -- The python programming language -- Data analysis -- Fundamentals of genome sequencing and annotation -- Fundamentals of proteomics -- Fundamentals of metabolomics -- Enzymes and receptors -- Drug discovery and development.…”
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Generation of stably expressing IRF5 spliced isoform in Jurkat cells
Published 2020-11-01“… Lentiviral transduction enables the generation of gain-of-function of a targeted gene in mammalian cells. Single-cell cloning through limiting dilution can establish a population of cells with homogenous transgene expression for exploring protein function. …”
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133
Tropical and Subtropical Fruit Propagation
Published 2019-11-01“…While sexual propagation (by seed) results in plants that are not genetically the same as the mother plant, asexual propagation (cuttings, division, air-layers, and grafting) creates offspring that are clones of the mother plant. Cloning fruit trees is important because it allows different cultivars to be preserved over time. …”
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134
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology /
Published 2018Table of Contents: “…Biochemical and molecular biological methods in life sciences studies -- Basic principles -- Cell culture techniques -- Recombinant DNA techniques and molecular cloning -- Preparative protein biochemistry -- Electrophoretic techniques -- Immunochemical techniques -- Flow cytometry -- Radioisotope techniques -- Principles of clinical biochemistry -- Microscopy -- Centrifugation and ultracentrifugation -- Spectroscopic techniques -- Basic techniques probing molecular structure and interactions -- Mass spectrometric techniques -- Fundamentals of bioinformatics -- Fundamentals of chemoinformatics -- The python programming language -- Data analysis -- Fundamentals of genome sequencing and annotation -- Fundamentals of proteomics -- Fundamentals of metabolomics -- Enzymes and receptors -- Drug discovery and development.…”
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135
International encyclopaedia of laws : Uganda /
Published 2021Table of Contents: “…Codex volumes : Bio-ethics (general) -- Human genome/cloning/human reproduction -- Removal and use of organs, cells and tissues -- Medical research on human beings -- Mental illness and psychiatry -- End of life -- The medical profession and the health care system -- Patients' rights.…”
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Reference Material -
136
Tropical and Subtropical Fruit Propagation
Published 2019-11-01“…While sexual propagation (by seed) results in plants that are not genetically the same as the mother plant, asexual propagation (cuttings, division, air-layers, and grafting) creates offspring that are clones of the mother plant. Cloning fruit trees is important because it allows different cultivars to be preserved over time. …”
Get full text
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137
Designing a broad-spectrum multi-epitope subunit vaccine against leptospirosis using immunoinformatics and structural approaches
Published 2025-01-01“…Immune simulations and in silico cloning were also conducted to evaluate the vaccine’s immune response and expression potential.ResultsThe MESV demonstrated high antigenicity, immunogenicity, non-allergenicity, and conservation across different Leptospira strains. …”
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138
Human Fetal Liver: An In Vitro Model of Erythropoiesis
Published 2011-01-01“…In this in vitro model, maturation that is enucleation reaches a lower level compared to adult sources as observed for embryonic or iP, but, interestingly, they (i) displayed a dramatic in vitro expansion (100-fold more when compared to CB CD34+) and (ii) 100% cloning efficiency in hematopoietic progenitor assays after 3 days of erythroid induction, as compared to 10–15% cloning efficiency for adult CD34+ cells. …”
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139
Synthesis of symmetric paths of arbiter physically unclonable function on FPGA
Published 2019-06-01“…Circuit design solutions in physical cryptography are called digital physically unclonable functions (PUFs), which to be implemented ensure the uniqueness, non-reproducibility (non-cloning) of the protected digital device. In addition, PUFs should be efficient as hardware resources. …”
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IL-1 Receptor Antagonist Protects the Osteogenesis Capability of Gingival-Derived Stem/Progenitor Cells under Inflammatory Microenvironment Induced by Porphyromonas gingivalis Lipo...
Published 2021-01-01“…Cell behaviors including proliferation, cloning formation unit (CFU), cell migration, osteogenic differentiation, mineral deposition, and cytokine production were assessed to investigate the protection effect of IL-1ra on GMSCs under inflammation. …”
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