Imaging Flow Cytometric Identification of Chromosomal Defects in Paediatric Acute Lymphoblastic Leukaemia by Ana P. A. Simpson, Carly E. George, Henry Y. L. Hui, Ravi Doddi, Rishi S. Kotecha, Kathy A. Fuller, Wendy N. Erber

“…There was variability in clonal makeup in individual cases, suggesting the presence of sub-clones. Copy number alterations and translocations could be detected even when the cell population comprised less than 1% of cells and included cells with a mature B-cell phenotype, i.e., CD19-positive, lacking CD34 and CD10. …”
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Development of ptxD/Phi as a new dominant selection system for genetic manipulation in Cryptococcus neoformans by Muthita Khongthongdam, Tanaporn Phetruen, Sittinan Chanarat

“…A codon-optimized ptxD gene from Pseudomonas stutzeri was cloned under the TEF promoter. Using the transient CRISPR-Cas9 coupled with electroporation system, we integrated the ptxD gene into the C. neoformans genome and assessed the impact of ptxD integration on cell growth and virulence factors. …”
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The MSDIN family in amanitin-producing mushrooms and evolution of the prolyl oligopeptidase genes by Hong Luo, Qing Cai, Yunjiao Lüli, Xuan Li, Rohita Sinha, Heather E. Hallen-Adams, Zhu L. Yang

“…In addition, two MSDIN sequences were cloned from L. brunneoincarnata basidiomes. The toxin MSDIN genes encoding amatoxins or phallotoxins from the three genera were compared, and a phylogenetic tree constructed. …”
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Prognostic value of matrix metalloproteinases and transforming growth factor – β in kidney cancer by A. I. Tarasenko, A. N. Rossolovskiy, O. L. Berezinets, A. B. Bucharskaya, S. S. Pakhomiy, A. O. Efimova, G. N. Maslyakova

“…All patients at the preoperative stage, in the early (7–10 days) and remote postoperative periods (after 1 and 2 years) were tested by solid‑phase ELISA on a StatFax 4200 analyzer using eBiosence and Cloud‑Clone Corp reagent kits for the serum concentration of oncogenesis markers MMP‑9 and TGF‑β1.Results. …”
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Investigation of <i>ITGB3</i> Heterogeneity to Overcome Trastuzumab Resistance in HER2-Positive Breast Cancer by Asiye Busra Boz Er

“…<i>ITGB3</i> expression varied significantly among resistant clones, correlating with increased expression of TGF-β-responsive genes and enhanced migration markers. …”
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Constructing the constitutively active ribosomal protein S6 kinase 2 from <i>Arabidopsis thaliana</i> (AtRPS6K2) and testing its activity <i>in vitro</i> by A. V. Zhigailov, G. E. Stanbekova, D. K. Beisenov, A. S. Nizkorodova, N. S. Polimbetova, B. K. Iskakov

“…In Arabidopsis thaliana, AtRPS6 is phosphorylated by kinase AtRPS6K2, which should in turn be phosphorylated by upper level kinases (AtPDK1 – at serine (S) 296, AtTOR – at threonine (T) 455 and S437) for full activation. We have cloned AtRPS6K2 cDNA gene and carried out in vitro mutagenesis replacing codons encoding S296, S437 and T455 by triplets of phosphomimetic glutamic acid (E). …”
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Effect of Learning Environment on Academic Performance of Pupils and Students in Kagango Division Sheema Municipality. by Andonzire, Steve James

“…The study sampling was cloned on ~l I lead teachers, 30 students, 8 Teachers: and 8 parents from Kagango Division which were both male and female. …”
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Disrupting USP39 deubiquitinase function impairs the survival and migration of multiple myeloma cells through ZEB1 degradation by Jessy Sirera, Saharnaz Sarlak, Manon Teisseire, Alexandrine Carminati, Victoria J. Nicolini, Coline Savy, Patrick Brest, Thierry Juel, Christophe Bontoux, Marcel Deckert, Mickael Ohanna, Sandy Giuliano, Maeva Dufies, Gilles Pages, Frederic Luciano

“…Despite advancements with proteasome inhibitors, immunomodulatory agents, and CD38-targeting antibodies, MM remains largely incurable due to resistant clones and frequent relapses. The success of the proteasome inhibitor bortezomib (BTZ) in MM treatment highlights the critical role of the ubiquitin–proteasome system (UPS) in this disease. …”
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Genomic Characterization of Carbapenemase-Producing Enterobacteriaceae from Clinical and Epidemiological Human Samples by Alexander Tristancho-Baró, Laura Eva Franco-Fobe, Monica Pilar Ariza, Ana Milagro, Ana Isabel López-Calleja, Blanca Fortuño, Concepción López, Miriam Latorre-Millán, Laura Clusa, Rosa Martínez, Carmen Torres, Antonio Rezusta

“…Advanced genomic tools enhance the detection of resistance genes, virulence factors, and high-risk clones, thus improving the management of MDR infections. …”
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High Antimicrobial Susceptibility of Cloacal Enterococci and <i>Escherichia coli</i> from Free-Living Dalmatian and Great White Pelicans with Detection of Cefotaximase CTX-M-15 Pro... by Teresa Cardona-Cabrera, Sandra Martínez-Álvarez, Carmen González-Azcona, Carlos Javier Gijón-García, Olga Alexandrou, Giorgos Catsadorakis, Panagiotis Azmanis, Carmen Torres, Ursula Höfle

“…<b>Conclusions:</b> Despite the susceptibility of most fecal <i>E. coli</i> and enterococci isolates to all antibiotics tested, the finding that <i>E. coli</i> of lineage ST69 carry <i>bla</i>_CTX-M-15 is of concern. This high-risk clone needs further investigation to elucidate its primary sources and address the growing threat of antimicrobial resistance from an integrated “One Health” perspective. …”
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Virulence profile of carbapenem-resistant Klebsiella pneumoniae strains by an in vivo model of Galleria mellonella by María Guembe, Rama Hafian, Marta Díaz-Navarro, Andrés Visedo, Flavio De Maio, Fulvia Pimpinelli, Ilaria Cavallo, Mauro Truglio, Francesca Sivori, Enea Gino Di Domenico

“…The most common sequence types (ST) identified were the high-risk clones ST307 (N = 10), ST512 (N = 8), ST101 (N = 7), and ST661 (N = 2). …”
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Population genetic analysis of clinical Mycobacterium abscessus complex strains in China by Xiangchen Li, Xiangchen Li, Yelei Zhu, Yewei Lu, Kunyang Wu, Yang Che, Xiaomeng Wang, Weixin Wang, Junli Gao, Junshun Gao, Zhengwei Liu, Zhengwei Liu, Zhuxian Zhou

“…BackgroundTo explore the genetic characteristics of the Mycobacterium abscessus complex (MABC) population in China, given its rising clinical importance among nontuberculous mycobacteria.MethodsWe conducted population genetic analyses on 360 MABC genomes from China, focusing on core genome multilocus sequence typing (cgMLST), pan-genome characterization, population genetics, and antimicrobial resistance gene profiling.ResultsOur analysis identified 273 M. abscessus subsp. abscessus (MabA) and 87 M. abscessus subsp. massiliense (MabM) isolates, uncovering 68 sequence types (STs), with ST5 being the most common. cgMLST classified 33.3% of isolates into six dominant circulating clones (DCCs) and 49.4% into 59 genomic clusters at a threshold of 25 different alleles, including 18 international clusters linking Chinese isolates with seven other countries. …”
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Potato mosaic viruses which infect plants of tuber-bearing Solanum spp. growing in the VIR field gene bank by E. V. Rogozina, N. V. Mironenko, N. A. Chalaya, Yu. Matsushita, H. Yanagisawa

“…No PSTVd was found in the studied plants, but a plural infestation by mosaic viruses was detected, more than half of the tested clones are infected with two or more viruses. In the studied samples, only 17 genotypes (12 %) are not infected by PVX, PVS, PVM and PVY according to the ELISA test. …”
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Phytosomes Loaded with Mastoparan-M Represent a Novel Strategy for Breast Cancer Treatment by Zhao H, Bao S, Chen S, Yang Q, Lou K, Gai Y, Lin J, Liu C, Liu H, Zhang C, Yang R

“…Its anti-tumor mechanism was investigated using CCK-8, clone formation, and apoptosis assays. Subsequently, the biodistribution and anti-tumor efficacy of Phy-Mast-M were assessed in vivo using the 4T1 tumor-bearing mouse model. …”
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The StbHLH47 transcription factor negatively regulates drought tolerance in potato (Solanum tuberosum L.) by Peijie Wang, Xiaojuan Wu, Nan Li, Hushuai Nie, Yu Ma, Juan Wu, Zhicheng Zhang, Yanhong Ma

“…Results In this study, the StbHLH47 gene, which is highly expressed in potato leaves, was cloned and isolated. Subcellular localization assays revealed that the gene StbHLH47 performs transcriptional functions in the nucleus, as evidenced by increased malondialdehyde (MDA) content and relative conductivity under drought stress. …”
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Predictive value of MMP-2, MMP-9, TIMP-1 in surgical treatment of localized renal cell cancer by A. I. Tarasenko, A. N. Rossolovskiy, O. L. Berezinets, D. A. Durnov, E. B. Popyhova, V. M. Popkov

“…All patients being in the early (7-10th day) and long-term postoperative period (after 1 and 2 years) by solid-phase ELISA, on a StatFax 4200 analyzer using eBiosence and Cloud-Clone Corp reagent kits, a study was made on the basis of the concentration in the blood serum of markers of oncogenesis MMP‑2, MMP‑9 and TIMP‑1 metalloproteinase inhibitor.Results. …”
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Phage-mediated virulence loss and antimicrobial susceptibility in carbapenem-resistant Klebsiella pneumoniae by Yanshuang Yu, Mengzhu Wang, Liuying Ju, Minchun Li, Mengshi Zhao, Hui Deng, Christopher Rensing, Qiu E. Yang, Shungui Zhou

“…Specifically, Kp2092 rapidly developed resistance to phages through mutations in a key phage receptor (galU) and bacterial membrane defenses such as LPS synthesis, however, this evolution coincides with unexpected benefits. Evolved bacterial clones not only exhibited increased sensitivity to clinically important antibiotics but also displayed a loss of virulence in an in-vivo model. …”
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A Novel Multiplex LAMP Assay for the Rapid and Accurate Diagnosis of Visceral Leishmaniasis Caused by Leishmania infantum from Iran by Sahar Samsami, Sahar Namavari, Saeed Ataei, Abdolmajid Ghasemian, Ava Yazdanpanah, Neda Sepahi, Gholamreza Hatam, Hossein Faramarzi, Hadi Mirzaei, Razie Ranjbar, Ali Ghanbariasad

“…All molecular methods were tested on the serial dilution of cloned plasmid containing ITS region from standard strains of L. infantum, L. tropica, and L. major. …”
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EPIGENETICS TODAY AND TOMORROW by B. F. Vanyushin

“…Epigenetic parameters are very significant for understanding of somaclonal variation mechanisms; characterization of clones and cell cultures, including stem cells at various differentiation stages; and determination of their differentiation directions. …”
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Shedding light on the existence of Furan fatty acids in latex lipids across a wide diversity of Hevea brasiliensis genotypes by Chaisiri Kitpaosong, Siriluck Liengprayoon, Erwann Durand, Tucksin Lerksamran, Aurélie Daval, Eric Gohet, Lim Khan Tiva, Phean Chetha, Kong Mengchheang, Phen Phearun, Ung Rattana, Kim Chandy, Pol Sokea, Un Kim Eng, Kittipong Rattanaporn, Bruno Barea, Laurent Vaysse

“…Based on knowledge of the parentage of the studied clones, a trait heritability study was conducted and genotype PB5/51 was identified as a very worthwhile genitor for improving furan fatty acid contents in a breeding population.…”
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