Genotyping <em> Escherichia coli </em> O157:H7 strains isolated from cattle and small ruminants in Fars province (Iran) using Enterobacterial Repetitive Intergenic Consensus PCR A... by Sedigheh Mokhtari, Yahya Tahamtan, Mohammad Kargar, Keyvan Tadayon, Elham Moazamian

“…Isolates from different hosts and sources exhibited similar patterns, indicating a high prevalence of specific clones in various hosts. The study revealed the genetic diversity of E. coli O157:H7 collected from identical sources. …”
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Long-term impact of molecular epidemiology shifts of methicillin-resistant Staphylococcus aureus on severity and mortality of bloodstream infection by Norihito Kaku, Masaki Ishige, Go Yasutake, Daisuke Sasaki, Kenji Ota, Fujiko Mitsumoto-Kaseida, Kosuke Kosai, Hiroo Hasegawa, Koichi Izumikawa, Hiroshi Mukae, Katsunori Yanagihara

“…Additionally, ST8-MRSA-IV was divided into CA-MRSA/J, t5071-ST8-MRSA-IV, and USA300-like clone based on the results of molecular analysis. …”
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Overexpression of <i>AtruLEA1</i> from <i>Acer truncatum</i> Bunge Enhanced <i>Arabidopsis</i> Drought and Salt Tolerance by Improving ROS-Scavenging Capability by Shaofeng Li, Huijing Meng, Yanfei Yang, Jinna Zhao, Yongxiu Xia, Shaoli Wang, Fei Wang, Guangshun Zheng, Jianbo Li

“…In this study, <i>LEA1</i> was cloned from <i>A. truncatum</i> Bunge and functionally characterized. …”
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The MADS-Box Transcription Factor ClAG2 Is a Key Regulator for the Formation of Double Flower in <i>Clematis</i> L. by Xuerong Chen, Ping Zhou, Nanhong Guo, Yiping Zheng, Xiumei Hou, Lihui Zeng

“…The open reading frame (ORF) of <i>ClAG2</i> was cloned and overexpression of <i>ClAG2</i> in tobacco resulted in shorter corolla tube, reduced crown area, and stunted stamen. …”
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Development and in vitro evaluation of biomimetic injectable hydrogels from decellularized human nerves for central nervous system regeneration by Gopal Agarwal, Kennedy Moes, Christine E. Schmidt

“…Human astrocytes, human microglial cell clone 3 (HMC3), and mouse RAW 264.7 macrophages were cultured individually within iHPN, with lipopolysaccharide (LPS) added to mimic CNS inflammation following injury. …”
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The concept of natural genome reconstruction. Part 1. Basic provisions of the “natural genome reconstruction” concept. Changing the genome of hematopoietic stem cells using several... by L. A. Yakubov, O. S. Taranov, S. V. Sidorov, S. D. Nikonov, A. A. Ostanin, E. R. Chernykh, N. A. Kolchanov, S. S. Bogachev

“…In this regard, there is a fundamental possibility of changing the hematopoietic status of hematopoietic stem cells in the direction of polyclonality and the original diversity of clones. Such events can form the basis for the rejuvenation of the blood-forming cell system. …”
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Construction and characterization of bioluminescent Salmonella Reading outbreak and non-outbreak strains by Abubakar Shitu Isah, Reshma Ramachandran, Anuraj Theradiyil Sukumaran, Aaron S. Kiess, Claudia D. Castañeda, Timothy Boltz, Kenneth S. Macklin, Hossam Abdelhamed, Li Zhang

“…The chloramphenicol acetyltransferase gene was cloned into the plasmid pBS-slpGFPluxABCDE, which carries luxABCDE operon and an ampicillin resistance gene. …”
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Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in <i>Lilium</i> ‘Siberia’ by Xiaoping Xu, Chenglong Yang, Yiping Zheng, Wenjie Guo, Zhongxiong Lai, Shaozhong Fang

“…<i>LoPurple acid phosphatase17</i> (<i>LoPAP17</i>), a pivotal gene of the riboflavin metabolism pathway, was subsequently cloned. LoPAP17 was most closely related to the orthologous genes of <i>Acorus calamus</i>, <i>Asparagus officinalis</i>, and <i>Musa acuminata</i>. …”
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Emergence of a blaNDM-5-carrying extensively drug-resistant Enterobacter cloacae ST1718 in Saudi Arabia: Insights from comprehensive genome analysis by Ibrahim A. Al-Zahrani, Thamer M. Brek

“…Conclusion: The findings highlight the need for monitoring the spread of multidrug-resistant clones, conducting molecular epidemiological studies, and implementing effective infection control measures to prevent the dissemination of antimicrobial resistance in healthcare settings.…”
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Metformin as an adjuvant option for systemic treatment of breast cancer by D.I. Avierin, V.F. Zavizion

“…However, it should be considered that more aggressive therapy of oncological diseases can be a nosocomial selector of more aggressive clones of the pool of tumor cells. The main questions are whether metformin can be a targeted drug for the treatment of tumor, whether it is appropriate to use it at the time when the manifestation of evolution disturbances of tumor cell is minimal and homogeneity is maximal.…”
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Molecular and functional characterization of Accl(2)efl: A biomarker for heavy metal stress in Apis cerana cerana by Yu-Lin Xu, Ling Peng, Jun-Jie Li, Wen-Feng Chen

“…To address this knowledge gap, we cloned the l(2)efl gene from Apis cerana cerana [Accl(2)efl] and conducted a bioinformatics analysis on the encoded protein, aiming to elucidate the potential functions of Accl(2)efl. …”
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South American species <i>Solanum alandiae</i> Card. and <i>S. okadae</i> Hawkes et Hjerting as potential sources of genes for potato late blight resistance by O. A. Muratova (Fadina), M. P. Beketova, M. A. Kuznetsova, E. V. Rogozina, E. E. Khavkin

“…Four accessions of S. alandiae, one accession of S. okadae and 11 clones of interspecific potato hybrids comprising S. alandiae germplasm from the VIR collection were PCR-screened using specific SCAR (Sequence Characterized Amplified Region) markers for eight Rpi genes. …”
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Expression and Function of Synthetic Dimeric Exercise-Induced Cytokine Irisin-Irisin by LIU Fengying, HUANG Yide, LIN Jia, CHEN Xuequn, WANG Qingshui, LIN Yao

“…The functions were verified in gastric cancer, colorectal cancer and other gastrointestinal tumors, and the activity and function of recombinant Irisin-Irisin and monomeric Irisin on tumor cells were compared.Results(1) Expression and purification of recombinant Irisin-Irisin: compared with the non-induced samples, the recombinant Irisin-Irisin was significantly expressed after IPTG induction; high purity recombinant Irisin-Irisin with a molecular weight of about 27 kDa was obtained by Ni Sepharose excel. (2) Detection of recombinant Irisin-Irisin activity: compared with the blank control group, both the recombinant Irisin-Irisin group and the Irisin group could up-regulate the expression of UCP-1 in C2C12 cells (<italic>P</italic>&lt;0.05); compared with the Irisin group, the recombinant Irisin-Irisin group had a more significant effect and stronger activity in inducing UCP-1 (<italic>P</italic>&lt;0.05). (3) Recombinant Irisin-Irisin on tumor function: compared with the blank control group, both the 100 ng/mL and 200 ng/mL Irisin-Irisin groups could inhibit the migration of gastric cancer cells and colorectal cancer cells(<italic>P</italic>&lt;0.05), but had no effect on their proliferation and clone formation; compared with the Irisin group, the recombinant Irisin-Irisin group had a more significant effect on cell migration inhibition (<italic>P</italic>&lt;0.05).ConclusionThe recombinant Irisin-Irisin expressed by <italic>Escherichia coli</italic> has activity, and the activity is higher than that of the monomeric Irisin; recombinant Irisin-Irisin has an effect on the migration of gastric cancer cells and colorectal cancer cells, and the effect was better than that of the monomeric Irisin.…”
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POPULATION GENETICS STUDY OF THE WHEAT LEAF RUST AGENT PUCCINIA TRITICINA IN DAGESTAN by E. I. Gultyaeva, E. L. Shaydayuk, K. M. Abdullaev

“…Infectious material was represented by  leaves of bread wheat with uredopustules collected in the experimental field of DES VIR in 2008, 2009, 2011, 2014, 2016, and 2017. Populations were cloned to obtain monopustule isolates. For  this and for virulence analysis, the laboratory technique of detached  leaves preserved in the benzimidazole solution was used. …”
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Construction of prokaryotic system for expression of porcine circovirus type 2 ORF-2 gene fragment by A. G. Galeeva, A. R. Akhunova, K. V. Usoltsev, N. I. Khammadov, M. A. Efimova

“…A genetic insert constructed from the most immunogenic type-specific PCV-2 epitopes based on genotype 2a, 2b, 2d strain and isolate consensus sequence was cloned into the expression vector pET-22b(+) that was incorporated into the Escherichia coli strain Rosetta 2(DE3). …”
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The <i>Meq</i> Genes of Nigerian Marek’s Disease Virus (MDV) Field Isolates Contain Mutations Common to Both European and US High Virulence Strains by Joseph N. Patria, Luka Jwander, Ifeoma Mbachu, Levi Parcells, Brian Ladman, Jakob Trimpert, Benedikt B. Kaufer, Phaedra Tavlarides-Hontz, Mark S. Parcells

“…Several of the <i>meq</i> genes were cloned for expression and their localization ability to interact with the chicken NF-IL3 protein, a putative Meq dimerization partner, were assessed. …”
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Galectin-3 secreted by triple-negative breast cancer cells regulates T cell function by Annat Raiter, Yael Barhum, Julia Lipovetsky, Chen Menachem, Sharona Elgavish, Shmuel Ruppo, Yehudit Birger, Shai Izraeli, Orna Steinberg-Shemer, Rinat Yerushalmi

“…Here, we further investigated the interaction between secreted Gal-3 and T cells in TNBC.Using CRISPR/Cas9 gene editing of the TNBC MDA-MB-231 cell-line, we obtained Gal-3 negative(neg) clones. We studied these in an in-vitro model, co-cultured with peripheral blood mononuclear cells (PBMC) to imitate immune-tumor interaction, and in an in-vivo model, when implanted in mice.Gal-3neg tumors in mice had decelerated tumor growth after PBMC inoculation. …”
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<i>Rubus idaeus RiACS1</i> Gene Is Involved in Ethylene Synthesis and Accelerates Fruit Ripening in <i>Solanum lycopersicum</i> by Tiemei Li, Wenjiao Xin, Hang Zhang, Jiarong Jiang, Kunmiao Ding, Mengyu Liu, Nanyan Li, Guohui Yang

“…In this study, the <i>RiACS1</i> gene in raspberry (<i>Rubus idaeus</i> L.) variety ‘Polka’ was cloned. The <i>RiACS1</i> gene overexpression vector was constructed and transformed into tomato plants using the <i>Agrobacterium tumefaciens</i> infection method to verify its function in their reproductive development. …”
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TaWI12 may be involved in pistillody and leaf cracking in wheat by Yuhuan Guo, Yan Zhang, Yuhao Li, Yichao Wu, Mingli Liao, Zhengsong Peng, Zaijun Yang, Yonghong Zhou

“…Consequently, investigating the developmental mechanisms of pistils and stamens in wheat is profoundly significant for enhancing wheat characteristics and boosting productivity. In this study, we cloned TaWI12, from common wheat and observed a significant resemblance among the three homoeologs of TaWI12. …”
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Detection of Antimicrobial Resistance Genes Associated with Carbapenem Resistance from the Whole-Genome Sequence of Acinetobacter baumannii Isolates from Malaysia by Mohan Rao, Fairuz A. Rashid, Surianti Shukor, Rohaidah Hashim, Norazah Ahmad

“…These findings predict that carriage of carbapenem-resistant genes possibly constitutes the underlying basis of high level of international clone II prevalence. Therefore, molecular surveillance and antimicrobial stewardship are essential in implementing policies to prevent and control the spread of CrAb in hospital settings.…”
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