The use of molecular-genetic and phytopathological methods to identify genes for effective leaf rust resistance in Aegilops accessions by L. G. Tyryshkin, M. A. Kolesova

“…In the phytopathological test, the seedlings were infected with the pathogen population (avirulent to Lr9 and Lr41 genes) and the fungus clones virulent to the wheat line with the Lr9 gene.Results and conclusions. …”
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Overcoming barriers for investigating nickel-pincer nucleotide cofactor-related enzymes by Jorge L. Nevarez, Aiko Turmo, Santhosh Gatreddi, Swati Gupta, Jian Hu, Robert P. Hausinger

“…Here, we show that when the Lactiplantibacillus plantarum lar genes are cloned into the Duet expression system and cultured in Escherichia coli, they confer lactate racemase activity to the cells. …”
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Light-chain proximal tubulopathy: a retrospective study from a single Chinese nephrology referral center by Xin Wang, Xiao-juan Yu, Su-xia Wang, Fu-de Zhou, Ming-hui Zhao

“…These patients showed improved or stable kidney function.Conclusions This is the first case series report of LCPT in four different pathological types in northern China. Clone-targeted chemotherapy may help preserve the kidney function in these patients.…”
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Features of the resource species Miscanthus sacchariflorus (Maxim.) Hack. when introduced in West Siberia by Yu. A. Gismatulina, V. V. Budaeva, G. V. Sakovich, O. Yu. Vasilyeva, G. A. Zueva, A. S. Gusar, O. V. Dorogina

“…Hence, it is preferable to cultivate seedlings on protected ground, and plantations are advisable to establish with more stable cloned vegetative material. The chemical compositions of the whole plant, leaf and stem separately, from seven M. sacchariflorus harvests were examined to reveal a rise in cellulose content and a drop in noncellulosic content with plantation age. …”
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Fatty acid metabolism-related signature suggests an oncogenic role of TEKT1 in endometrial cancer by Zhikai Xiahou, Hong Xiao, Yafeng Song, Xin Xu

“…Cellular proliferation, clone formation, migration, invasion, cycle, and apoptosis were assessed to detect the effect of TEKT1 in endometrial cancer. …”
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Localization of rust resistance genes in old local Russian flaxes by methods of classical genetics by S. N. Kutuzova, E. A. Porokhovinova, N. B. Brutch, A. V. Pavlov

“…F2 hybrids in the phase  of cotyledon leaves were inoculated with monopustule clones  of the  fungus,  not  virulent  to any of evaluated genes.  …”
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Metagenomic characterization of oral microbiome signatures to predict upper gastrointestinal and pancreaticobiliary cancers: a case–control study by Sujin Oh, Jaihwan Kim, Cheol Min Shin, Hyo-Jung Lee, Hye Seung Lee, Kyoung Un Park

“…For GC, the classifier exhibited high discriminative power (AUC = 0.961); it included five genera (Escherichia-Shigella, Gemella, Holdemanella, Actinomyces, and Stomatobaculum) and three species (Eubacterium sp. oral clone EI074, Ruminococcus sp. Marseille-P328, and Leptotrichia wadei F0279). …”
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Problems and prospects of grapevine genetic resources preservation at “Magarach” Institute by A. A. Polulyakh, V. A. Volynkin, V. V. Likhovsko

“…At the institute “Magarach” we have formed a vegetative collection of 40 grapevine varieties, hybrids and clones in vitro, and this work is under way. An effective way to solve the problem of gene pool preservation is cryopreservation, which is the main way of preserving genetic material of some crops. …”
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Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies by A. N. Chikaev, A. P. Rudometov, Yu. A. Merkulyeva, L. I. Karpenko

“…The biopanning procedure allows isolation of specific clones for almost any target, and due to the physical link between the genotype and the phenotype of recombinant phage particles it is possible to determine the structure of selected molecules. …”
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Design and expression of a chimeric recombinant antigen (SsIR-Ss1a) for the serodiagnosis of human strongyloidiasis: Evaluation of performance, sensitivity, and specificity. by Mostafa Omidian, Zohreh Mostafavi-Pour, Marzieh Asadi, Meysam Sharifdini, Navid Nezafat, Ali Pouryousef, Amir Savardashtaki, Mortaza Taheri-Anganeh, Fattaneh Mikaeili, Bahador Sarkari

“…The construct was expressed into the pET-23a (+) expression vector and the optimized DNA sequences of SsIR-Ss1a (873 bp) were cloned into competent E. coli DH5α cells. Diagnostic performances of the produced recombinant antigen, along with a commercial kit were evaluated in an indirect ELISA system, using a panel of sera from strongyloidiasis patients and controls. …”
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USING <i> AVENA MACROSTACHYA </i> FOR IMPROVEMENT OF OAT WINTERHARDINESS IN POLAND by B. . Lapinski, A. . Rachwalska

“…Large differences were noticed in fertility level and chromosome constitution of progeny of the intensely cloned and colchicine treated F1 hybrids. The B1F or F2 generation consisted of plants with chromosome numbers between 40 and 49, octoploids (2n=56) and plants carrying 60 to 70 chromosomes. …”
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Variation in Botryosphaeriaceae from Eucalyptus plantations in YunNan Province in southwestern China across a climatic gradient by Guoqing Li, Bernard Slippers, Michael J. Wingfield, Shuaifei Chen

“…All the Botryosphaeriaceae species were pathogenic to one-year-old plants of an E. urophylla × E. grandis clone and E. globulus seed-derived plants, but showed significant inter- and intra-species variation in aggressiveness amongst isolates. …”
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Importance of OsRac1 in Signalling of Pigm-1 Mediated Resistance to Rice Blast Disease by Dewei Yang, Niqing He, Fenghuang Huang, Jialin Chen, Minxiang Yu, Yidan Jin, Shaojun Lin, Shengping Li

“…The NLR protein Pigm-1 was cloned from Shuangkang 77009 in our laboratory. This study shows that the nucleotide-binding site (NBS) domain of Pigm-1 facilitates its binding to and activation of OsRac1 while the coiled-coil (CC) domain enables its binding to and activation of RAI1, ultimately inducing cell death. …”
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Structure- and machine learning-guided engineering demonstrate that a non-canonical disulfide in an anti-PD-1 rabbit antibody does not impede antibody developability by Wei-Ching Liang, Hongkang Xi, Dawei Sun, Luigi D’Ascenzo, Jonathan Zarzar, Nicole Stephens, Ryan Cook, Yinyin Li, Zhengmao Ye, Marissa Matsumoto, Jian Payandeh, Matthieu Masureel, Yan Wu

“…First, guided by the sequence of a related clone and the crystal structure of h1340.CC in complex with PD-1, we generated variant h1340.SA.LV with a potency and cross-reactivity similar to h1340.CC, but only partially recovered affinity. …”
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Microwave hyperthermia enhances radiosensitization by decreasing DNA repair efficiency and inducing oxidative stress in PC3 prostatic adenocarcinoma cells by Yajun Wu, Pengyuan Liu, Wendy Chen, Shiting Bai, Sisi Chen, Jianglin Chen, Xiaogang Xu, Jindan Xia, Yufei Wu, Jianjun Lai, Chuan Sun, Zhenghong Lao, Xiaoqing Wan, Zhibing Wu

“…Western blotting was used to detect DNA damage repair protein expression in PC3 cells and transplanted tumors.Results Compared to control, PC3 cell survival and clone formation rates decreased in RT + MW-HT group, demonstrating significant increase in apoptosis, ROS levels, and DNA damage. …”
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Localization and expression of C1QTNF6 in chicken follicles and its regulatory effect on follicular granulosa cells by Chong Li, Qi Li, Xing Wu, Zihao Zhang, Jing Li, Ruirui Jiang, Guoxi Li, Xiaojun Liu, Xiangtao Kang, Zhuanjian Li, Donghua Li, Yadong Tian

“…In this study, the full-length coding sequence (CDS) of chicken C1QTNF6 was cloned from adult chicken ovary. We demonstrated that the putative C1QTNF6 protein shares a highly conserved amino acid sequence with known bird homologs. …”
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Development and evaluation of a recombinant monoclonal human antibody with virus-neutralizing activity against the F glycoprotein of respiratory syncytial virus by Sergey A. Klotchenko, Ekaterina A. Romanovskaya-Romanko, Marina A. Plotnikova, Anastasia A. Pulkina, Aram A. Shaldzhyan, Dmitry S. Balabashin, Victoria A. Toporova, Teimur K. Aliev, Natalia E. Gyulikhandanova, Dmitry A. Lioznov

“…The authors are currently developing a stable FM1 producer clone with high productivity and viability and investigating the therapeutic efficacy of this antibody in a sublethal RSV infection mouse model.…”
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Artificial intelligence-based spatial analysis of tertiary lymphoid structures and clinical significance for endometrial cancer by Haruka Suzuki, Kohei Hamada, Junzo Hamanishi, Akihiko Ueda, Ryusuke Murakami, Mana Taki, Rin Mizuno, Koichi Watanabe, Hanako Sato, Yuko Hosoe, Hiroaki Ito, Koji Yamanoi, Hiroyuki Yoshitomi, Nobuyuki Kakiuchi, Ken Yamaguchi, Noriomi Matsumura, Seishi Ogawa, Hideki Ueno, Masaki Mandai

“…B cell receptor repertoire analysis performed using six TLS samples located at various distances from the tumor showed that TLSs in distal areas had more shared B cell clones with tumor-infiltrating lymphocytes. To comprehensively investigate the distribution of TLSs, we developed an artificial intelligence model to detect TLSs and determine their spatial locations in whole-slide images. …”
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Understanding the mechanisms of hypoxia-induced tissue damage in fish: The role of GasderminEa/b in Larimichthys crocea by Lin Wang, Shuaiyu Chen, Yongyang Wang, Weini Zhang, Yinnan Mu, Yudong Jia, Changtao Guan, Meng Xue, Xinhua Chen

“…In the present study, two Lcgsdme genes, Lcgsdmea/b, were cloned and characterized from the large yellow croaker. …”
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The association of single nucleotide polymorphisms in tumor necrosis factor 3 with susceptibility/resistance of Magallana gigas to Halomonas sp. 7T by Hongmei Fan, Qingyu Peng, Tianbao Liu, Lei Fang, Chuanyan Yang, Ning Kong, Lingling Wang, Linsheng Song

“…The gene coding region sequence of MgTNF-3 gene from two oyster populations (the bacterial-resistant population and the common population) was cloned, and the polymorphisms within this region were investigated to analyze their association with bacterial resistance. …”
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