Anatomy, ploidy level, and essential oil composition of <i>Hyssopus officinalis</i> ʻNikitskiy Beliyʼ <i>in vitro</i> and <i>ex situ</i> by I. V. Bulavin, N. N. Ivanova, N. N. Miroshnichenko, N. M. Saplev, S. A. Feskov

“…The developed protocol for clonal micropropagation of Hyssopus officinalis ʻNikitskiy Beliyʼ provides clones identical to the ex situ plants.…”
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Leaf spot resistance in sweet and sour cherries by M. S. Lenivtseva, A. P. Kuznetsova, E. E. Radchenko

“…Seedling 3-20-5-23 (P. serrulata × Northstar) was not resistant to a number of C. hiemalis clones. It is assumed that this genotype and Vetrovoe 11 (P. kurilensis) are protected by identical alleles of resistance genes, which differ from the alleles present in the five new hybrid forms.…”
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Mutagenesis and Identification of Sugarcane Mutants Using Survival on Polyethylene Glycol and Leaf Damage under Managed Water Stress by Napa Weksanthia, Tanapon Chaisan, Wannasiri Wannarat, Songyos Chotchutima, Peeranuch Jompuk

“…Thus, EMS mutagenesis and evaluation using in vitro and greenhouse methods were successful in developing new sugarcane clones with high water-stress tolerance, which is important for sugarcane breeding programs.…”
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New genetic resources in wheat breeding for an increased grain protein content by O. P. Mitrofanova, A. G. Khakimova

“…For the first time, a functional locus, or the Gpc-B1 gene (chromosome 6BS) affecting the accumulation of protein, Zn and Fe in grain, was identified in T. dicoccoides, cloned and studied in detail. The application of molecular markers has revealed the active allele of this gene in some landraces and old cul-tivars of T. dicoccum, T. durum, T. spelta and T. aestivum. …”
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Hepatitis B virus genotypes A1 and A2 have distinct replication phenotypes due to polymorphisms in the HBx gene. by Min Zhang, Karim Mouzannar, Zhensheng Zhang, Yuji Teraoka, Jason Piotrowski, Yuji Ishida, Chise Tateno-Mukaidani, Takeshi Saito, Hiromi Abe-Chayama, Kazuaki Chayama, T Jake Liang

“…Using HBV generated from molecule clones of subtypes A1 and A2 in cell culture (HBVcc), we demonstrate that HBVcc of subtypes A1 and A2 can be passaged in vitro and in vivo and respond equally well to human IFN-α treatment. …”
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Influence of allelic polymorphism in the 3’ untranslated region of the <i>StTCP23</i> gene on the tolerance of potato cultivars to the potato spindle tuber viroid by N. V. Mironenko, A. V. Kochetov, O. S. Afanasenko

“…For each cultivar, six clones of each cDNA amplicon of StTCP23 with a 3’ UTR were identified, and the allelic compositions of the target regions within their 3’ UTRs were characterized.Results. …”
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Plexin C1 Marks Liver Cancer Cells with Epithelial Phenotype and Is Overexpressed in Hepatocellular Carcinoma by Gorkem Odabas, Metin Cetin, Serdar Turhal, Huseyin Baloglu, A. Emre Sayan, Tamer Yagci

“…A monoclonal antibody, clone PE4, specific to Plexin C1, was generated. …”
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Identification of site-specific adaptations conferring increased neural cell tropism during human enterovirus 71 infection. by Samuel Cordey, Tom J Petty, Manuel Schibler, Yannick Martinez, Daniel Gerlach, Sandra van Belle, Lara Turin, Evgeny Zdobnov, Laurent Kaiser, Caroline Tapparel

“…Comparison of five full-length genomes sequenced directly from respiratory, gastrointestinal, nervous system, and blood specimens revealed three nucleotide changes that occurred within a five-day period: a non-conservative amino acid change in VP1 located within the BC loop (L97R), a region considered as an immunogenic site and possibly important in poliovirus host adaptation; a conservative amino acid substitution in protein 2B (A38V); and a silent mutation in protein 3D (L175). Infectious clones were constructed using both BrCr (lineage A) and the clinical strain (lineage C) backgrounds containing either one or both non-synonymous mutations. …”
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Listeria monocytogenes use multiple mechanisms to disseminate from the intestinal lamina propria to the mesenteric lymph nodes by Joshua S. Nowacki, Grant S. Jones, Sarah E. F. D'Orazio

“…To block CCR7-mediated trafficking of dendritic cells from the lamina propria, we treated mice with anti-VEGFR3 antibody (clone AFL4) prior to and during infection but did not see a change in dendritic numbers in the MLN as had been previously reported with other anti-VEGFR3-specific antibodies. …”
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Dual Reporter Gene Imaging by Jiantu Che, Mikhail Doubrovin, Inna Serganova, Ludmila Ageyeva, Pat Zanzonico, Ronald Blasberg

“…The human and rodent sodium iodide symporters ( NIS ) have recently been cloned and are being investigated as potential therapeutic and reporter genes. …”
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Development of IgY-Based Sandwich ELISA as a Robust Tool for Rapid Detection and Discrimination of Toxigenic Vibrio cholerae by Mahdiye Bayat, Alireza Khabiri, Behzad Hemati

“…The sequences of ompW and ctxB genes were cloned into pET28a vector. Escherichia coli BL21 (DE3) was transformed with the recombinant vectors, and gene expression was induced by IPTG. …”
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Current status and perspectives of hematopoietic cell transplantation in patients with paroxysmal nocturnal hemoglobinuria by Marek Ussowicz, Dawid Przystupski, Patrycja Mensah-Glanowska, Agnieszka Piekarska

“…In the case of severe aplastic anemia with an associated PNH clone, immunoablative protocols based on anti-thymocyte globulin serotherapy with fludarabine and cyclophosphamide are recommended. …”
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The genomic pattern of insertion/deletion variations during rice improvement by Xia Zhou, Jilong Li, Lei Chen, Minjie Guo, Renmin Liang, Yinghua Pan

“…Using phenotypic data from five agronomic traits (heading date, flag leaf length, flag leaf width, panicle number, and plant height) along with INDEL data to perform genome-wide association study (GWAS), we identified 6,331 significant loci involving 157 cloned genes. Haplotype analysis of candidate genes revealed INDELs affecting important functional genes, such as OsMED25 and OsRRMh related to heading date, and MOC2 related to plant height. …”
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Comparative diagnostic performance of recombinant Tams1 protein based dot-ELISA in detecting tropical theileriosis in naturally infected cattle by Sanjhi Paliwal, Vikrant Sudan, Daya Shanker

“…The recombinant Tams1 protein was successfully cloned and expressed using a pET-30b (+) expression vector in a prokaryotic system. …”
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Identification and characterization of CONSTANS-like (COL) gene family in upland cotton (Gossypium hirsutum L.). by Darun Cai, Hui Liu, Na Sang, Xianzhong Huang

“…Tissue-specific expression showed that 42 GhCOL genes may function as tissue-specific regulators in different cells or organs. We cloned and sequenced the 14 GhCOL genes in Group I related to flowering induction to study their diurnal expression pattern, and found that their expression showed distinct circadian regulation. …”
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Nuclear DNA Content and Chromatin Pattern of Rat Rhabdomyosarcoma Cell Sublines with Different Metastatic Potentials by Jean Dufer, Marie-France Poupon, Sonia Yatouji

“…The aim of this work was to characterize nuclear changes observed in metastatic cell clones issued in vitro from the same parental cell population The nuclear phenotypes of 6 cell sublines isolated from a rat rhabdomyosarcoma cell line and differing in their metastatic ability were evaluated by image cytometry on Feulgen‐stained preparations. …”
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A New Functional Model for Prediction of Chaperone Activity of the Recombinant M. tb Acr (α-Crystallin) Using Insulin as Substrate by Gautam Krishnan, Utpal Roy

“…In this study, Mycobacterium alpha crystallin (acr) gene was cloned and expressed in Escherichia coli (E. coli). …”
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<i>Triticum durum</i> Desf. is a valuable source for spring bread wheat genetic diversity enhancement by A. E. Druzhin, S. N. Sibikeev, E. I. Gultyaeva, L. B. Andreeva

“…Introgression of chromatin from durum wheat cultivars into the spring bread wheat gene pool made it possible to produce lines resistant to the local P. triticina population, as well as to some test clones of this pathogen. Such lines can be used as donors of the Lr genes. …”
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Sporoderm-Removed Spore Powder May Suppress the Proliferation, Migration, and Invasion of Esophageal Squamous Cell Carcinoma Cells Through PI3K/AKT/mTOR and Erk Pathway by Jingyan Liu Master degree, Tao Zeng Doctor degree

“…The MTS assay was used to assess the esophageal squamous cell carcinoma cells viability. The clone formation assay was used to evaluate to the proliferation ability of KYSE140 and KYSE510 cells. …”
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Core-genome guided novel therapeutic targets identification and chimeric vaccine designing against Rickettsia rickettsii by Fizza Arshad, Asifa Sarfraz, Muhammad Shehroz, Umar Nishan, Asia Perveen, Riaz Ullah, Mohamed A. Ibrahim, Mohibullah Shah

“…The vaccine constructs were cloned and expressed in suitable systems. This study displays a comprehensive strategy for managing Rocky Mountain Spotted Fever via rational vaccine development. …”
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