The curated Lactobacillus acidophilus NCFM genome provides insights into strain specificity and microevolution by Meichen Pan, Sarah O’Flaherty, Ashley Hibberd, Svetlana Gerdes, Wesley Morovic, Rodolphe Barrangou

“…Results Here, we revisit the L. acidophilus genome, which was sequenced twenty years ago, and determined the core and pan genomes of 114 publicly available L. acidophilus strains, spanning commercial isolates, academic strains and clones from the scientific literature. Results indicate a predictable high level of homogeneity within the species, but also reveal surprising mis-assemblies. …”
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Phage display technology in ecotoxicology: phage display derived unique peptide for copper identification in aquatic samples by Marta Sosnowska, Tomasz Łęga, Marcin Olszewski, Beata Gromadzka

“…To identify a variety of Cu-binding M13 phage clones with unique peptides and to assess their affinity for metal ions, the study utilized Escherichia coli as a factories producing recombinant bacteriophages, modified biopanning procedure and an ELISA assay. …”
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The <i>Pyrus sinkiangensis Yu PsLEA4</i> Gene Enhances the Cold Resistance of <i>Solanum lycopersicum</i> by Xueying Yang, Wenjuan Zhao, Hui Li, Zhenxia Zhao, Jianbo Zhu, Jin Li

“…In order to evaluate the biological function of the <i>PsLEA4</i> protein under low-temperature stress and its potential for use in agricultural breeding, we cloned the <i>PsLEA4</i> gene from the Korla Pear, made a plant overexpression vector, and transformed it into a tomato via Agrobacterium transformation. …”
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Isolation, Characterization, and Molecular Modeling of a Rheumatoid Factor from a Hepatitis C Virus Infected Patient with Sjögren’s Syndrome by Yu-Ching Lee, Keng-Chang Tsai, Sy-Jye Leu, Tuan-Jen Wang, Chia-Yu Liu, Yi-Yuan Yang

“…After panning, a specific clone RFL11 was isolated for characterization in advance. …”
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Genetic assessment in early clonal population in sugarcane (Saccharum officinarum L.) for productivity traits and resistance to foliar diseases by B. Shivarudra1, Sanjay B. Patil2, P. K. Mallikarjun3*, H. G. Manoj Kumar1 and N. G. Hanamaratti4

“…The results revealed significant variability within and between families, heritability and genetic advance for all traits. Clones such as SNK 190023, SNK 191722, SNK 190362 and SNK 191801 recorded significantly higher cane yield besides showing high resistance (HR) to foliar diseases such as brown rust, red leaf spot, eye spot, brown spot and pokkah boeng in comparison to the checks Co 09004 (early) and Co 86032 (mid-late). …”
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Alterations of Hematologic and Hematopoietic Parameters in Mice Exposed to Pulsed Electromagnetic Field by Kangchu Li, Zenghui Teng, Zhaohui Liu, Yuxing Zhang, Kaiping Long, Qimei Liao, Ansheng Ni, Guirong Ding, Shirong Ma

“…There was no significant difference in mouse bone marrow nucleated cell number between the control group and each exposed group 7 days after PEMF exposure. The CFU-GM clone number of bone marrow cells and serum GM-CSF level were significantly increased in the 100 and 1000 pulses exposed group but not changed in the 10000 pulses exposed group. …”
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New insights into the mechanism of triphenyl phosphate and its metabolite diphenyl phosphate in diabetic kidney disease by Ting Fang, Qiaoyan Liu, Xinxin Huangfu, Hongkai Zhu, Hongwen Sun, Liming Chen

“…Toxicity analysis showed that TPHP could cause dose-dependent toxicity in mouse podocyte clone 5 (MPC5) and mouse mesangial cells (MES13). …”
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Oral Immunotherapy Using Probiotic Ice Cream Containing Recombinant Food-Grade Lactococcus lactis Which Inhibited Allergic Responses in a BALB/c Mouse Model by Alireza Vasiee, Fereshteh Falah, Mojtaba Sankian, Farideh Tabatabaei-Yazdi, Seyed Ali Mortazavi

“…The main gene of this allergen, Ama r 2, was cloned into the food grade plasmid pNZ7025 and then was electrotransformed into the food grade Lactococcus lactis NZ1330. …”
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A High-Quality Phased Genome Assembly of Stinging Nettle (<i>Urtica dioica</i> ssp. <i>dioica</i>) by Kaede Hirabayashi, Christopher R. Dumigan, Matúš Kučka, Diana M. Percy, Gea Guerriero, Quentin Cronk, Michael K. Deyholos, Marco Todesco

“…Here, we present a chromosome-level, phased genome assembly for a diploid female clone of <i>Urtica dioica</i> from Romania. Using a combination of PacBio HiFi, Oxford Nanopore, and Illumina sequencing, as well as Hi-C long-range interaction data (using a novel Hi-C protocol presented here), we assembled two haplotypes of 574.9 Mbp (contig N50 = 10.9 Mbp, scaffold N50 = 44.0 Mbp) and 521.2 Mbp (contig N50 = 13.5 Mbp, scaffold N50 = 48.0 Mbp), with assembly BUSCO scores of 92.6% and 92.2%. …”
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Spatio‐Temporal Patterns of Hybridization in an Alloploid Salamander (Ambystomatidae: Ambystoma) and Conservation Implications of Introgression in a Unisexual Vertebrate by Alessa V. Laserna, Christopher A. Phillips, Andrew R. Kuhns, Mark A. Davis, Joel B. Corush, Ken N. Paige

“…Gynogenetic reproduction involving LJJ and its host A. texanum should result in a clone of the mother (LJJ); however, with the occurrence of kleptogenesis, it often can result in tetraploid hybrids (LJJT). …”
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Genomic, Evolutionary, and Pathogenic Characterization of a New <i>Polerovirus</i> in Traditional Chinese Medicine <i>Viola philippica</i> by Yuanling Chen, Gaoxiang Chen, Jiaping Yu, Yali Zhou, Shifang Fei, Haorong Chen, Jianxiang Wu, Shuai Fu

“…Two potential recombination events were identified within ORF2, ORF3a, and ORF3, providing insights into the genetic diversity and evolution history of this novel polerovirus. An infectious cDNA clone of VPPV was successfully constructed and shown to infect <i>Nicotiana benthamiana</i>. …”
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A prognostic model of 8-T/B cell receptor-related signatures for hepatocellular carcinoma by Xuan Zuo, Hui Li, Shi Xie, Mengfen Shi, Yujuan Guan, Huiyuan Liu, Rong Yan, Anqi Zheng, Xueying Li, Jiabang Liu, Yifan Gan, Haiyan Shi, Keng Chen, Shijie Jia, Guanmei Chen, Min Liao, Zhanhui Wang, Yanyan Han, Baolin Liao

“…Both the number of unique TCR and BCR clonotypes and the expanded clones were higher in the low-risk group than in the high-risk group. …”
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Uptake of CCR7 by KIR2DS4+ NK Cells Is Induced upon Recognition of Certain HLA-C Alleles by Silvia Pesce, Simona Carlomagno, Alessandro Moretta, Simona Sivori, Emanuela Marcenaro

“…We demonstrate that KIR2DS4 can induce the uptake of CCR7 by KIR2DS4+ NKG2A+ NK cell clones after interacting with CCR7+ target cells expressing HLA-Cw4 and HLA-Cw6 alleles. …”
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Melanocortin Peptide Therapy for the Treatment of Arthritic Pathologies by S. J. Getting, M. Kaneva, Y. Bhadresa, D. Renshaw, Giovanna Leoni, H. B. Patel, M. J. P. Kerrigan, I. C. Locke

“…Their biological effects are mediated via seven transmembrane G-protein-coupled receptors, of which five have been cloned, identified, and termed MC1 to MC5. Adrenocorticotrophic hormone represents the parent molecule of the melanocortins; the first 13 amino acids of which (termed a-MSH) have been shown to be the most pharmacologically active region of the parent hormone. …”
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In vivo interspecies dissemination of IncM2-type blaNDM-1 carrying plasmid by Mor N. Lurie-Weinberger, Darya Bychenko-Banyas, Meirav Mor, Sivan Laviad-Shitrit, Ella Kaplan, Nadya Rakovitsky, Alona Keren-Paz, Chaim Ben-Zvi, Yehuda Carmeli

“…Such events may cause the emergence of high-risk clones, as well as lead to under-recognized multi-species outbreaks.IMPORTANCEConjugative, carbapenemase-carrying multidrug-resistant plasmids that can move between species of clinically relevant Enterobacterales pose a great risk to patients’ health, especially when they spread inside a medical institution. …”
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Heterologous expression of SpsTAC2 in Arabidopsis affected branch angle and secondary vascular system development by Fangshu Niu, Mengru Yuan, Hongxia Zhao, Zhi Pang, Jie Yan, RuiXie Ning, Lin Shi, Fengqiang Yu, Dongshan Wei, Rong Yang, Runming Zhang, Haifeng Yang

“…In this study, TAC2 was cloned from Salix psammophila, and an overexpression and subcellular localization expression vector for the SpsTAC2 gene was constructed. …”
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Landscape of the Peripheral Immune Response Induced by Intraoperative Radiotherapy Combined with Surgery in Early Breast Cancer Patients by Danian Dai, Xuerui Li, Hongkai Zhuang, Yun Ling, Lezi Chen, Cheng Long, Jinhui Zhang, Yunjie Wang, Yuehua Li, Hailin Tang, Bo Chen

“…Communication between CD8Teff_GZMK cells and other immune cells via MIF_CD74 and MIF_TNFRSF14 is decreased after IORT. cDCs showed an upregulation of the MCH II signaling pathway, while memory B cells exhibited enhanced activation of the B cell pathway. T cell clones expanded significantly after treatment. IORT+Surgery demonstrated the ability to partially suppress the anti‐tumor effects of neutrophils. …”
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Multi-omics analysis reveals that neutrophil extracellular traps related gene TIMP1 promotes CRC progression and influences ferroptosis by Yuzhao Jin, Luyu Liao, Qianping Chen, Bufu Tang, Jin Jiang, Ji Zhu, Minghua Bai

“…We used small interfering RNA to knockdown TIMP1, and verified the ability of TIMP1 in CRC proliferation, invasion and migration through western blot, transwell, cell scratch assay, cell clone formation and other experiments. Result We screened out three major NETs Genes: TIMP1, F3, and CRISPLD2 based on machine learning. …”
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The Progression of Mycosis Fungoides During Treatment with Mogamulizumab: A BIO-MUSE Case Study of the Tumor and Immune Response in Peripheral Blood and Tissue by Angelica Johansson, Eirini Kalliara, Emma Belfrage, Teodor Alling, Paul Theodor Pyl, Anna Sandström Gerdtsson, Urban Gullberg, Anna Porwit, Kristina Drott, Sara Ek

“…<b>Conclusions:</b> We propose that in patients with MF, the evolution of the malignant clone and the associated immune response need to be studied jointly to define relevant strategies for intervention.…”
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Single cell transcriptome profiling reveals pathogenesis of bullous pemphigoid by Guirong Liang, Chenjing Zhao, Qin Wei, Suying Feng, Yetao Wang

“…Blood CX3CR1+ ZNF683+ and LAG3+ exhausted T cells exhibit the highest TCR expansion among clones shared with skin CD8+T cells, suggesting their role in fueling skin CD8+T cell clonal expansion. …”
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