Emergence of a blaNDM-5-carrying extensively drug-resistant Enterobacter cloacae ST1718 in Saudi Arabia: Insights from comprehensive genome analysis by Ibrahim A. Al-Zahrani, Thamer M. Brek

“…Conclusion: The findings highlight the need for monitoring the spread of multidrug-resistant clones, conducting molecular epidemiological studies, and implementing effective infection control measures to prevent the dissemination of antimicrobial resistance in healthcare settings.…”
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Genotyping <em> Escherichia coli </em> O157:H7 strains isolated from cattle and small ruminants in Fars province (Iran) using Enterobacterial Repetitive Intergenic Consensus PCR A... by Sedigheh Mokhtari, Yahya Tahamtan, Mohammad Kargar, Keyvan Tadayon, Elham Moazamian

“…Isolates from different hosts and sources exhibited similar patterns, indicating a high prevalence of specific clones in various hosts. The study revealed the genetic diversity of E. coli O157:H7 collected from identical sources. …”
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Molecular and functional characterization of Accl(2)efl: A biomarker for heavy metal stress in Apis cerana cerana by Yu-Lin Xu, Ling Peng, Jun-Jie Li, Wen-Feng Chen

“…To address this knowledge gap, we cloned the l(2)efl gene from Apis cerana cerana [Accl(2)efl] and conducted a bioinformatics analysis on the encoded protein, aiming to elucidate the potential functions of Accl(2)efl. …”
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South American species <i>Solanum alandiae</i> Card. and <i>S. okadae</i> Hawkes et Hjerting as potential sources of genes for potato late blight resistance by O. A. Muratova (Fadina), M. P. Beketova, M. A. Kuznetsova, E. V. Rogozina, E. E. Khavkin

“…Four accessions of S. alandiae, one accession of S. okadae and 11 clones of interspecific potato hybrids comprising S. alandiae germplasm from the VIR collection were PCR-screened using specific SCAR (Sequence Characterized Amplified Region) markers for eight Rpi genes. …”
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The MADS-Box Transcription Factor ClAG2 Is a Key Regulator for the Formation of Double Flower in <i>Clematis</i> L. by Xuerong Chen, Ping Zhou, Nanhong Guo, Yiping Zheng, Xiumei Hou, Lihui Zeng

“…The open reading frame (ORF) of <i>ClAG2</i> was cloned and overexpression of <i>ClAG2</i> in tobacco resulted in shorter corolla tube, reduced crown area, and stunted stamen. …”
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POPULATION GENETICS STUDY OF THE WHEAT LEAF RUST AGENT PUCCINIA TRITICINA IN DAGESTAN by E. I. Gultyaeva, E. L. Shaydayuk, K. M. Abdullaev

“…Infectious material was represented by  leaves of bread wheat with uredopustules collected in the experimental field of DES VIR in 2008, 2009, 2011, 2014, 2016, and 2017. Populations were cloned to obtain monopustule isolates. For  this and for virulence analysis, the laboratory technique of detached  leaves preserved in the benzimidazole solution was used. …”
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Construction of prokaryotic system for expression of porcine circovirus type 2 ORF-2 gene fragment by A. G. Galeeva, A. R. Akhunova, K. V. Usoltsev, N. I. Khammadov, M. A. Efimova

“…A genetic insert constructed from the most immunogenic type-specific PCV-2 epitopes based on genotype 2a, 2b, 2d strain and isolate consensus sequence was cloned into the expression vector pET-22b(+) that was incorporated into the Escherichia coli strain Rosetta 2(DE3). …”
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Structure- and machine learning-guided engineering demonstrate that a non-canonical disulfide in an anti-PD-1 rabbit antibody does not impede antibody developability by Wei-Ching Liang, Hongkang Xi, Dawei Sun, Luigi D’Ascenzo, Jonathan Zarzar, Nicole Stephens, Ryan Cook, Yinyin Li, Zhengmao Ye, Marissa Matsumoto, Jian Payandeh, Matthieu Masureel, Yan Wu

“…First, guided by the sequence of a related clone and the crystal structure of h1340.CC in complex with PD-1, we generated variant h1340.SA.LV with a potency and cross-reactivity similar to h1340.CC, but only partially recovered affinity. …”
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Localization and expression of C1QTNF6 in chicken follicles and its regulatory effect on follicular granulosa cells by Chong Li, Qi Li, Xing Wu, Zihao Zhang, Jing Li, Ruirui Jiang, Guoxi Li, Xiaojun Liu, Xiangtao Kang, Zhuanjian Li, Donghua Li, Yadong Tian

“…In this study, the full-length coding sequence (CDS) of chicken C1QTNF6 was cloned from adult chicken ovary. We demonstrated that the putative C1QTNF6 protein shares a highly conserved amino acid sequence with known bird homologs. …”
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Development and evaluation of a recombinant monoclonal human antibody with virus-neutralizing activity against the F glycoprotein of respiratory syncytial virus by Sergey A. Klotchenko, Ekaterina A. Romanovskaya-Romanko, Marina A. Plotnikova, Anastasia A. Pulkina, Aram A. Shaldzhyan, Dmitry S. Balabashin, Victoria A. Toporova, Teimur K. Aliev, Natalia E. Gyulikhandanova, Dmitry A. Lioznov

“…The authors are currently developing a stable FM1 producer clone with high productivity and viability and investigating the therapeutic efficacy of this antibody in a sublethal RSV infection mouse model.…”
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Artificial intelligence-based spatial analysis of tertiary lymphoid structures and clinical significance for endometrial cancer by Haruka Suzuki, Kohei Hamada, Junzo Hamanishi, Akihiko Ueda, Ryusuke Murakami, Mana Taki, Rin Mizuno, Koichi Watanabe, Hanako Sato, Yuko Hosoe, Hiroaki Ito, Koji Yamanoi, Hiroyuki Yoshitomi, Nobuyuki Kakiuchi, Ken Yamaguchi, Noriomi Matsumura, Seishi Ogawa, Hideki Ueno, Masaki Mandai

“…B cell receptor repertoire analysis performed using six TLS samples located at various distances from the tumor showed that TLSs in distal areas had more shared B cell clones with tumor-infiltrating lymphocytes. To comprehensively investigate the distribution of TLSs, we developed an artificial intelligence model to detect TLSs and determine their spatial locations in whole-slide images. …”
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Understanding the mechanisms of hypoxia-induced tissue damage in fish: The role of GasderminEa/b in Larimichthys crocea by Lin Wang, Shuaiyu Chen, Yongyang Wang, Weini Zhang, Yinnan Mu, Yudong Jia, Changtao Guan, Meng Xue, Xinhua Chen

“…In the present study, two Lcgsdme genes, Lcgsdmea/b, were cloned and characterized from the large yellow croaker. …”
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The association of single nucleotide polymorphisms in tumor necrosis factor 3 with susceptibility/resistance of Magallana gigas to Halomonas sp. 7T by Hongmei Fan, Qingyu Peng, Tianbao Liu, Lei Fang, Chuanyan Yang, Ning Kong, Lingling Wang, Linsheng Song

“…The gene coding region sequence of MgTNF-3 gene from two oyster populations (the bacterial-resistant population and the common population) was cloned, and the polymorphisms within this region were investigated to analyze their association with bacterial resistance. …”
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The <i>Meq</i> Genes of Nigerian Marek’s Disease Virus (MDV) Field Isolates Contain Mutations Common to Both European and US High Virulence Strains by Joseph N. Patria, Luka Jwander, Ifeoma Mbachu, Levi Parcells, Brian Ladman, Jakob Trimpert, Benedikt B. Kaufer, Phaedra Tavlarides-Hontz, Mark S. Parcells

“…Several of the <i>meq</i> genes were cloned for expression and their localization ability to interact with the chicken NF-IL3 protein, a putative Meq dimerization partner, were assessed. …”
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Galectin-3 secreted by triple-negative breast cancer cells regulates T cell function by Annat Raiter, Yael Barhum, Julia Lipovetsky, Chen Menachem, Sharona Elgavish, Shmuel Ruppo, Yehudit Birger, Shai Izraeli, Orna Steinberg-Shemer, Rinat Yerushalmi

“…Here, we further investigated the interaction between secreted Gal-3 and T cells in TNBC.Using CRISPR/Cas9 gene editing of the TNBC MDA-MB-231 cell-line, we obtained Gal-3 negative(neg) clones. We studied these in an in-vitro model, co-cultured with peripheral blood mononuclear cells (PBMC) to imitate immune-tumor interaction, and in an in-vivo model, when implanted in mice.Gal-3neg tumors in mice had decelerated tumor growth after PBMC inoculation. …”
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<i>Rubus idaeus RiACS1</i> Gene Is Involved in Ethylene Synthesis and Accelerates Fruit Ripening in <i>Solanum lycopersicum</i> by Tiemei Li, Wenjiao Xin, Hang Zhang, Jiarong Jiang, Kunmiao Ding, Mengyu Liu, Nanyan Li, Guohui Yang

“…In this study, the <i>RiACS1</i> gene in raspberry (<i>Rubus idaeus</i> L.) variety ‘Polka’ was cloned. The <i>RiACS1</i> gene overexpression vector was constructed and transformed into tomato plants using the <i>Agrobacterium tumefaciens</i> infection method to verify its function in their reproductive development. …”
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TaWI12 may be involved in pistillody and leaf cracking in wheat by Yuhuan Guo, Yan Zhang, Yuhao Li, Yichao Wu, Mingli Liao, Zhengsong Peng, Zaijun Yang, Yonghong Zhou

“…Consequently, investigating the developmental mechanisms of pistils and stamens in wheat is profoundly significant for enhancing wheat characteristics and boosting productivity. In this study, we cloned TaWI12, from common wheat and observed a significant resemblance among the three homoeologs of TaWI12. …”
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Detection of Antimicrobial Resistance Genes Associated with Carbapenem Resistance from the Whole-Genome Sequence of Acinetobacter baumannii Isolates from Malaysia by Mohan Rao, Fairuz A. Rashid, Surianti Shukor, Rohaidah Hashim, Norazah Ahmad

“…These findings predict that carriage of carbapenem-resistant genes possibly constitutes the underlying basis of high level of international clone II prevalence. Therefore, molecular surveillance and antimicrobial stewardship are essential in implementing policies to prevent and control the spread of CrAb in hospital settings.…”
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Imaging Flow Cytometric Identification of Chromosomal Defects in Paediatric Acute Lymphoblastic Leukaemia by Ana P. A. Simpson, Carly E. George, Henry Y. L. Hui, Ravi Doddi, Rishi S. Kotecha, Kathy A. Fuller, Wendy N. Erber

“…There was variability in clonal makeup in individual cases, suggesting the presence of sub-clones. Copy number alterations and translocations could be detected even when the cell population comprised less than 1% of cells and included cells with a mature B-cell phenotype, i.e., CD19-positive, lacking CD34 and CD10. …”
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The MSDIN family in amanitin-producing mushrooms and evolution of the prolyl oligopeptidase genes by Hong Luo, Qing Cai, Yunjiao Lüli, Xuan Li, Rohita Sinha, Heather E. Hallen-Adams, Zhu L. Yang

“…In addition, two MSDIN sequences were cloned from L. brunneoincarnata basidiomes. The toxin MSDIN genes encoding amatoxins or phallotoxins from the three genera were compared, and a phylogenetic tree constructed. …”
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