lncRNA ZFAS1 Promotes HMGCR mRNA Stabilization via Binding U2AF2 to Modulate Pancreatic Carcinoma Lipometabolism by Luoluo Wang, Yi Ruan, Xiang Wu, Xinhua Zhou

“…Measurements of BxPC-3 cell multiplication and invasiveness employed CCK-8, plate clone formation test, and Transwell chamber assay. …”
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Scutellarin combined with lidocaine exerts antineoplastic effect in human glioma associated with repression of epidermal growth factor receptor signaling. by Xiu-Ying He, Yui-Si Yang, Yue-Xiang Zheng, Qing-Jie Xia, Hong-Zhou Yu, Xiao-Ming Zhao, Ting-Hua Wang

“…Cell proliferation was detected by cell counting kit-8 (CCK8), plate clone formation assay, EdU incorporation assay and xCELLigence real-time cell analyzer. …”
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Characterization of Nora Virus Structural Proteins via Western Blot Analysis by Brad L. Ericson, Darby J. Carlson, Kimberly A. Carlson

“…ORF3, ORF4a, ORF4b, and ORF4c were individually cloned and expressed in E. coli; resultant recombinant proteins purified and were used to make monospecific antisera. …”
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The Conservative Physiology of the Immune System. A Non-Metaphoric Approach to Immunological Activity by Nelson M. Vaz, Gustavo C. Ramos, Vitor Pordeus, Claudia R. Carvalho

“…In the late 50s, selective theories were proposed and from then on, immunology has been based in a close association with the neo-Darwinian principles, such as random generation of variants (lymphocyte clones), selection by extrinsic factors (antigens)—and, more generally, on genetic determinism and functionalism. …”
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A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies by Huifang Chen, Zehong Zou, Ailin Tao

“…The splenocytes of immunized mice were fused with myeloma cells (SP2/0), and stable mAb-producing clones were obtained by limiting dilution. mAbs against Ara h 2 were isolated from mouse ascites, and specificity was confirmed by immunoblotting. …”
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Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism by Yihua Zhan, Jing Wang, Xuan Zhao, Zhifu Zheng, Yi Gan

“…Results Herein, AhMAGL3b encoding a protein with both hydrolase and acyltransferase regions, a member of MAGL gene family, was cloned and overexpressed in Arabidopsis thaliana. …”
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Purification and expression of a novel bacteriocin, JUQZ-1, against Pseudomonas syringae pv. Actinidiae (PSA), secreted by Brevibacillus laterosporus Wq-1, isolated from the rhizos... by Yang Shuai, Yi Langbo, Yang Yi, Chen Danni, Peng Qingzhong

“…The gene of the 01021 protein was cloned into the plasmid pPICZa, and the recombinant bacteriocin was successfully expressed using the Pichia pastoris X33 expression system. …”
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Donors of effective genes for scald resistance in barley by G. S. Konovalova, E. E. Radchenko

“…The experiments employed isolates and clones of R. commune isolated from plants collected in Leningrad Province. …”
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Are Ancient Durum Wheats Less Toxic to Celiac Patients? A Study of α-Gliadin from Graziella Ra and Kamut by M. Stella Colomba, Armando Gregorini

“…For all accessions, a few α-gliadin alleles were also cloned, sequenced and translated into aminoacid sequences. …”
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Effect of Codon Optimisation on the Production of Recombinant Fish Growth Hormone in Pichia pastoris by Hussin A. Rothan, Teh Ser Huy, Zulqarnain Mohamed

“…The optimized FGH coding sequence (oFGH) and native sequence (nFGH) of giant grouper fish (Epinephelus lanceolatus) were cloned into P. pastoris expression vector (pPICZαA) downstream of alcohol oxidase gene (AOX1) for efficient induction of extracellular rFGH by adding 1% of absolute methanol. …”
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基于全外显子组测序对头皮皮脂腺癌的基因分析 by 郑奔容, 王一娜, 江博雄, 梁亚乐, 蔡胜军, 张娜娜

“…筛选可疑的单核苷酸变异位点，进行突变的保守性和功能分析。利用SciClone软件来追踪亚克隆进化可以得到每例肿瘤样本的克隆性图谱信息。通过MutSigCV软件筛选得到高频显著基因，将体细胞变异与已知驱动基因进行比较，筛选出该肿瘤样本中的已知驱动基因。…”
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<i>Ex situ</i> conservation of <i>Rubus</i> L. (Rosaceae) genetic resources (a review) by S. E. Dunaeva, L. S. Krasovskaya, T. A. Gavrilenko

“…Wild species are mainly represented by those collected in the Russian North – 49 clones of red raspberry (Rubus idaeus L.) and 6 of cloudberries (R. chamaemorus L.) – and in the Caucasus (35 accessions of 26 species). …”
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Intratumor heterogeneity: models of malignancy emergence and evolution by R. A. Ivanov, S. A. Lashin

“…This knowledge enables the development of innovative strategies for targeting specific tumor clones, minimizing the risk of recurrence and improving patient outcomes. …”
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Inotodiol induces hepatocellular carcinoma apoptosis by activation of MAPK/ERK pathway. by Yushuang Xing, Di Jia, Xinping Zhu, Jialu Yang, Zhipeng Gao, Nana Meng, Haohao Xu, Mengxiao Wang, Shijun Chang, Mingqian Zhao, Shanbo Zhang, Zichen Mu, Qiang Tang, Weiming Zhao

“…The CCK8 assay was utilized to assess cell viability, the scratch assay was utilized to detect migration ability, the clone formation assay was utilized to detect clonogenic ability, and flow cytometry was utilized to analyze apoptosis and cell cycle. …”
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Status of the First Permanent Molars in Students from the José Antonio Saco Elementary School by Diosky Ferrer Vílchez, Ana Belkys Hernández Millán, Disney Tablada Peralta, Ana Ivis Betancourt García, Antonio Araujo Vílchez, Yenly Rodríguez Alonso

“…<br /><strong>Conclusion:</strong> the status of the first permanent molars can be generally classified as favorable, which was demonstrated through Clone’s index. A history of dental caries was a predisposing risk factor for the development of this condition; poor hygiene, presence of deep grooves and parental ignorance were also significant.…”
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Expression evaluation of exogenous and endogenous alcohol dehydrogenase genes in transgenic Arabidopsis by Haoqiang Yu, Hong Lv, Fengzhong Lu, Qingqing Yang, Huaming Duan, Wanchen Li, Fengling Fu

“…To detect the differences in expression, protein accumulation, and enzyme activity between transformed exogenous and endogenous genes, the coding sequences (CDSs) of the alcohol dehydrogenase (ADH) genes were cloned from dicotyledonous Arabidopsis, monocotyledonous maize, and prokaryotic Escherichia coli, constructed into expression vector pBI121-cMycNY, and used to transform wild-type Arabidopsis, respectively. …”
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De novo transcriptome assembly and discovery of drought-responsive genes in white spruce (Picea glauca). by Zoé Ribeyre, Claire Depardieu, Julien Prunier, Gervais Pelletier, Geneviève J Parent, John Mackay, Arnaud Droit, Jean Bousquet, Philippe Nolet, Christian Messier

“…We assembled a de novo transcriptome, performed differential gene expression analyses at four time points over 22 days during a controlled drought stress experiment involving 2-year-old plants and three genetically distinct clones, and conducted gene enrichment analyses. The transcriptome assembly and gene expression analysis identified a total of 33,287 transcripts corresponding to 18,934 annotated unique genes, including 4,425 genes that are uniquely responsive to drought. …”
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Genetic control of scald resistance in barley landraces by O. N. Soboleva, G. S. Konovalova, E. E. Radchenko

“…Analysis of the interaction between the pathogen test clones and the host plant revealed a difference between the alleles determining fungal resistance in 32 barley forms, the previously effective genes Rrs4, rrs6, rrs7, and currently effective Rrs9. …”
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Characterization of carbapenem-resistant Klebsiella pneumoniae from blood cultures in Gaza Strip hospitals, Palestine by Nahed Al Laham, Ahmed Al Afifi, Alexander Mellmann, Frieder Schaumburg

“…Conclusion: The proportion of CRKP among all K. pneumoniae from bloodstream infection in Gaza Strip is high (40 %) and mainly associated with the blaNDM-5-positive high-risk clone ST147.…”
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Investigating the Existence of Ribosomal Protein L5 Gene in Syrian Strain of Leishmania tropica Genome: Sequencing It and Evaluating Its Immune Response as DNA Vaccine by Mohammad Maarouf, Alyaa A. Abdlwahab

“…After proving the existence of the ribosomal protein L5 gene in a Syrian strain of Leishmania tropica (LCED Syrian 01), it was sequenced and cloned into a pCI plasmid, and the designed DNA vaccine was administered to BALB/c mice. …”
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