Standardization of Micropropagation for Four Hybrids of <i>Coffea arabica</i> Through Direct Somatic Embryogenesis by Marileydi Zuta-Puscan, Jegnes Benjamín Meléndez-Mori, Eyner Huaman-Huaman, Yoiner Kalin Lapiz-Culqui, Reyna Stefani Mego-Pérez, Manuel Oliva-Cruz

“…Direct somatic embryogenesis represents a fundamental tool for obtaining genetically homogeneous clones; however, its commercial scaling faces critical challenges at various stages of the process. …”
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Molecular Characterization and Expression of <i>unc-13d</i> in the Sex Reversal of <i>Monopterus albus</i> by Zitong Lian, Fang Meng, Xueping Xia, Junchao Fang, Haifeng Tian, Qiaomu Hu

“…In this study, we firstly cloned and characterized the sequence and protein structure of <i>unc-13d</i> of <i>M. albus.…”
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Epidemiology and Genetic Diversity of Colistin Nonsusceptible Nosocomial Acinetobacter baumannii Strains from Russia for 2013-2014 by Eugene A. Sheck, Mikhail V. Edelstein, Marina V. Sukhorukova, Natali V. Ivanchik, Elena Yu. Skleenova, Andrey V. Dekhnich, Ilya S. Azizov, Roman S. Kozlov

“…Among the colistin nonsusceptible A. baumannii isolates, 6 individual genotypes were identified, most of which belonged to successful international clones (CC92OXF/CC2PAS, n=4; CC944OXF/ST78PAS, n=4; CC109OXF/CC1PAS, n=1).…”
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The Chloroplastic Small Heat Shock Protein Gene KvHSP26 Is Induced by Various Abiotic Stresses in Kosteletzkya virginica by Xiaohua Liu, Lizi Zhao, Jianzhao Li, Lijun Duan, Kai Zhang, Xuqiang Qiao, Weihuan Li, Chengchao Zheng, Xiaoli Tang, Hongxia Zhang

“…In this work, a putative sHSP gene KvHSP26 was cloned from K. virginica. Bioinformatics analyses revealed that KvHSP26 encoded a chloroplastic protein with the typical features of sHSPs. …”
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Molecular Diversity of Sapovirus Infection in Outpatients Living in Nanjing, China (2011–2013) by Hong-ying Zhang, Meng-kai Qiao, Xuan Wang, Min He, Li-min Shi, Guo-xiang Xie, Hei-ying Jin

“…The PCR products were cloned into pGEM-T Easy vector and bidirectionally sequenced. …”
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Prolactin Levels Correlate with Abnormal B Cell Maturation in MRL and MRL/lpr Mouse Models of Systemic Lupus Erythematosus-Like Disease by Maria Victoria Legorreta-Haquet, Rocio Flores-Fernández, Francisco Blanco-Favela, Ezequiel M Fuentes-Pananá, Luis Chávez-Sánchez, Rafael Hernández-González, Emiliano Tesoro-Cruz, Lourdes Arriaga-Pizano, Adriana Karina Chávez-Rueda

“…These results identify an important role of PRL in the early stages of the B-cell maturation process: PRL may promote the survival of self-reactive clones.…”
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Improvement of the technique applied to preserve species and varieties of <i>Fragaria</i> L. in the field genebank at Krymsk Experiment Breeding Station of VIR by V. N. Podorozhniy, N. A. Piyanina

“…The accessions are sources and donors of traits important for breeding and represent the most valuable material for targeted crossings in programs developing and releasing strawberry cultivars, primarily garden ones.The effectiveness of their utilization can be seen in the adaptable high-yielding cultivars developed at Krymsk Station in recent years: ‘Zenkora’ (Zenga-Zengana × Cardinal) was patented, and ‘Pelageya’ [Elizaveta II (clone Coroleva Elizaveta) × Irma] included in the State Register of the Russian Federation. …”
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Yerba mate (Ilex paraguariensis) genome provides new insights into convergent evolution of caffeine biosynthesis by Federico A Vignale, Andrea Hernandez Garcia, Carlos P Modenutti, Ezequiel J Sosa, Lucas A Defelipe, Renato Oliveira, Gisele L Nunes, Raúl M Acevedo, German F Burguener, Sebastian M Rossi, Pedro D Zapata, Dardo A Marti, Pedro Sansberro, Guilherme Oliveira, Emily M Catania, Madeline N Smith, Nicole M Dubs, Satish Nair, Todd J Barkman, Adrian G Turjanski

“…Characterization of the genome allowed us to clone the genes encoding methyltransferase enzymes that catalyse multiple reactions required for caffeine production. …”
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Use of Posttranscription Gene Silencing in Squash to Induce Resistance against the Egyptian Isolate of the Squash Leaf Curl Virus by Omnia Taha, Inas Farouk, Abdelhadi Abdallah, Naglaa A. Abdallah

“…In this study full-length genomic clone of SqLCV Egyptian isolated and posttranscriptional gene silencing (PTGS) has been induced to develop virus resistance. …”
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CAR T cells for treating autoimmune diseases by Dimitrios Mougiakakos, Ulrike Koehl, Stephan Fricke, Ulrich Blache, Sandy Tretbar

“…Furthermore, in preclinical models, researchers have also developed chimeric autoantibody receptor T cells that can eliminate individual B cell clones producing specific autoantibodies, and regulatory CAR T cells that do not eliminate autoreactive immune cells but dampen their wrong activation. …”
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Bioinformatics Analysis, Prokaryotic Expression and Biological Activity of Lysin from Vibrio parahaemolyticus Phage vB_VpP_1 by ZHANG Defu, YANG Wenjing, LIU Ke, LIU Qingqing, BAI Wutong, LI Fan, LÜ Xinran, BAI Xue, TAN Xiqian, LI Xuepeng, LI Jianrong

“…Primers were designed using Primer 5.0 software and cloned into the pET-28a(+) vector for prokaryotic expression. …”
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Clonal Populations of Amniotic Cells by Dilution and Direct Plating: Evidence for Hidden Diversity by Patricia G. Wilson, Lorna Devkota, Tiffany Payne, Laddie Crisp, Allison Winter, Zhan Wang

“…More than 40 clonal populations were recovered from 4 amniocentesis samples and representative clones were characterized by flow cytometry, conventional assays for differentiation potential, immunofluorescence imaging, and transcript analysis. …”
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Identification and Characterization of a Novel Rat MAVS Variant Modulating NFκB Signaling by Ihsan Nalkiran, Hatice Sevim Nalkiran

“…In this study, we identified and characterized a novel rat MAVS variant (MAVS500) with a twenty-one-nucleotide deletion, resulting in a protein seven amino acids shorter than the wild-type (WT) rat MAVS. The MAVS500 was cloned from the rat bladder cancer cell line, NBT-II, using specific primers, and subsequently sequenced. …”
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Molecular Characterization of Antibiotic Resistance and Genetic Diversity of Klebsiella pneumoniae Strains by Bahareh Arabzadeh, Zeynab Ahmadi, Reza Ranjbar

“…Also, the results obtained from genotyping indicated that the K. pneumoniae strains isolated in this study belonged to the diverse clones.…”
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A phenomenological model of non-genomic variability of luminescent bacterial cells by S. I. Bartsev

“…All experiments were carried out with bacterial clones (genetically homogeneous populations). A typical dynamics of luminous bacterial cells distributions with respect to intensity of light emission at various stages of batch culture growth in a liquid medium was obtained. …”
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Novel approaches in myelofibrosis by Steffen Koschmieder

“…In addition, preclinical approaches that target individual mutated clones, for example, by mutation‐specific JAK2V617F inhibitors or DNA repair pathway inhibitors, are presented. …”
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Determination of mutagenicity of chemical compounds, physical factors and environmental pollutants by the Drosophila melanogaster wing somatic mutation and recombination test by L. P. Zakharenko, I. K. Zakharov

“…Recombination or mutational events in the cell leads to the formation of mutant spots/clones visible by microscopic analysis of the wing surface. …”
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Caracterización, procesabilidad y viabilidad técnica de fondos de taza de caucho natural by Natalia Cardona Vásquez, Diego Hernán Giraldo Vásquez, Sandra Milena Velásquez Restrepo

“… En este artículo, se compararon propiedades fsicoquímicas, espectroscópicas, de resistencia a la termooxidación y de procesabilidad de fondos de taza de caucho natural de los clones IAN 873 y FX 3864 respecto del caucho natural técnicamente especifcado grado 10 proveniente de Guatemala, uno de los más importados en Colombia y en América Latina. …”
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DISEASES OF NATURAL RUBBER (HEAVEA BRASILENSIS) INDUCED BY THE CLIMATIC ELEMENTS OF RAINFALL AND TEMPERATURE IN IKPOBA-OKHA LOCAL GOVERNMENT AREA OF EDO STATE, NIGERIA by ABRAHAM EHIZOJIE ILENRE

“…The following recommendations are made: effective disease management strategies, monitoring of climatic conditions, incorporating resistant natural rubber clones and improving cultural practices, sustainable practices to manage diseases, governments’ intervention to encouragenatural rubber farmers annually. …”
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Physically non-cloneable arbiter-type function with non-linear path pairs by A. A. Ivaniuk, A. Y. Shamyna

“…The set of all challenge-response pairs is a random, unpredictable and irreproducible in the cases of implementation of cloned PUF circuits both on single and/or on another chips, also using different technologies. …”
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