Variation in Botryosphaeriaceae from Eucalyptus plantations in YunNan Province in southwestern China across a climatic gradient by Guoqing Li, Bernard Slippers, Michael J. Wingfield, Shuaifei Chen

“…All the Botryosphaeriaceae species were pathogenic to one-year-old plants of an E. urophylla × E. grandis clone and E. globulus seed-derived plants, but showed significant inter- and intra-species variation in aggressiveness amongst isolates. …”
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Visual and High-Efficiency Secretion of SARS-CoV-2 Nanobodies with <i>Escherichia coli</i> by Shuai Zhao, Wanting Zeng, Fang Yu, Pingping Xu, Chin-Yu Chen, Wanping Chen, Yanming Dong, Fei Wang, Lixin Ma

“…In addition, <i>E. coli</i> cells secreting the nanobodies on their surface facilitates screening and characterization of antigen-binding clones.…”
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Discovery and in vitro characterization of a human anti-CD36 scFv by Cecilia Mata-Cruz, Cecilia Mata-Cruz, Sandra L. Guerrero-Rodríguez, Keyla Gómez-Castellano, Gregorio Carballo-Uicab, Juan Carlos Almagro, Juan Carlos Almagro, S. Mayra Pérez-Tapia, S. Mayra Pérez-Tapia, S. Mayra Pérez-Tapia, Marco A. Velasco-Velázquez

“…Herein, we aimed to identify human anti-CD36 single-chain variable fragment (scFv) with therapeutic potential.MethodsThe semisynthetic ALTHEA Gold Plus Libraries™ were panned using recombinant human CD36. Clone selection was performed by ELISA. Analysis of scFv binding and blocking function was evaluated by flow cytometry in macrophage-like THP-1 cells and hepatocellular carcinoma HepG2 cells. …”
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High hemolytic activity in Staphylococcus aureus t1081/ST45 due to increased hla protein production and potential RNAIII-independent regulation by Yu-Tzu Lin, Ngoc-Niem Bui, Yu-Syuan Cheng, Cheng-Wen Lin, Chun-Li Lee, Tai-Fen Lee, Po-Ren Hsueh

“…Sequence type (ST) 45 is a globally spread clone with increasing clinical prevalence in Taiwan. …”
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Diversity of new Russian bread wheat cultivars according to leaf rust resistance genes by E. I. Gultyaeva, E. L. Shaydayuk, V. V. Veselova, R. E. Smirnova, E. V. Zuev, A. G. Khakimova, O. P. Mitrofanova

“…Juvenile resistance in the seedling phase was assessed with two test clones (kLr9 и kLr19) and the Krasnodar population of Puccinia triticina Erikss. …”
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Overexpression of OsDUF868.12 enhances salt tolerance in rice by Hao Chen, Jiale Wan, Jiali Zhu, Ziyi Wang, Caiyao Mao, Wanjing Xu, Juan Yang, Yijuan Kong, Xiaofei Zan, Rongjun Chen, Jianqing Zhu, Zhengjun Xu, Lihua Li, Lihua Li

“…The Domain of Unknown Function 868 (DUF868) family, comprising 15 members in rice, has been identified in the protein family database. In this study, we cloned and functionally characterized OsDUF868.12, a member of the OsDUF868 family, to elucidate its role in rice response to salt stress. …”
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Sotorasib resistance triggers epithelial-mesenchymal transition and activates AKT and P38-mediated signaling by Raquel Arantes Megid, Guilherme Gomes Ribeiro, Izabela Natalia Faria Gomes, Ana Carolina Laus, Letícia Ferro Leal, Letícia Ferro Leal, Luciane Sussuchi da Silva, Abu-Bakr Adetayo Ariwoola, Josiane Mourão Dias, Rui Manuel Reis, Rui Manuel Reis, Renato Jose da Silva-Oliveira, Renato Jose da Silva-Oliveira

“…The aim of this study was to generate a sotorasib-resistant cell line via selective pressure and systematically examine the molecular and phenotypic alterations caused by resistance.MethodsMutant NCI-H358 (KRASG12C) were exposed to incremental doses (2–512 nM) of sotorasib. Then, resistant clones were separated by single-cell sorting. Proliferation was analyzed in real-time by xCELLigence; protein profiles were quantified by protein arrays; and mRNA expression profile was measured using the PanCancer Pathways panel by NanoString. …”
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Heterogeneity of cancer-associated fibroblast subpopulations in prostate cancer: Implications for prognosis and immunotherapy by Chen Ding, Jiange Wang, Jie Wang, Jiqiang Niu, Zhikai Xiahou, Zhou Sun, Zhenzhen Zhao, Dongyang Zeng

“…In vitro experiments verified that the T transcription factor NFAT5 of C1 ABCA8+ fibroblasts subpopulation was knocked down in LNCaP clone FGC and 22Rv1 cell lines, which was closely related to the proliferation of PC. …”
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Drug risk Factors Associated with a Sustained Outbreak of Clostridium difficile Diarrhea in a Teaching Hospital by Swapan K Nath, Suzette Salama, Devia Persaud, James H Thornley, Ian Smith, Gary Foster, Coleman Rotstein

“…In total, 80 cases associated with an endemic clone of toxigenic C difficile were compared with controls. …”
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High-Affinity Anti-VISTA Antibody Protects against Sepsis by Inhibition of T Lymphocyte Apoptosis and Suppression of the Inflammatory Response by Tianzhu Tao, Lulong Bo, Teng Li, Longbao Shi, Hui Zhang, Bo Ye, Yuhai Xu, Qingqing Ma, Xiaoming Deng, Guorong Zhang

“…The current study was designed to determine the potential therapeutic effects of a high-affinity anti-VISTA antibody (clone MH5A) in a murine model of sepsis. Methods. …”
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Colorectal carcinogenesis in the Lynch syndromes and familial adenomatous polyposis: trigger events and downstream consequences by Pål Møller, Aysel Ahadova, Matthias Kloor, Toni T. Seppälä, John Burn, Saskia Haupt, Finlay Macrae, Mev Dominguez-Valentin, Gabriela Möslein, Annika Lindblom, Lone sunde, Ingrid Winship, Gabriel Capella, Kevin Monahan, Daniel D. Buchanan, D. Gareth Evans, Eivind Hovig, Julian R. Sampson

“…Interventions focused on downstream events are external factors that influence which tumour clones survive Darwinian selection. In Lynch Syndrome, surveillance colonoscopy to remove adenomas may select for carcinogenetic pathways that bypass the adenoma stage.…”
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The cancer-associated fibroblasts interact with malignant T cells in mycosis fungoides and promote the disease progression by Yige Zhao, Yong Li, Yong Li, Panpan Wang, Mengyan Zhu, Jiaqi Wang, Bo Xie, Chenyu Tang, Yangyang Ma, Shiwen Wang, Sha Jin, Jinhui Xu, Zhao Li, Xiaoyan Zhang, Liuyu Li, Xiuzu Song, Ping Wang

“…We compared skin lesions in different stages within the same patient to overcome inter-individual variability.ResultsThe malignant clones displayed dual phenotypes characterized with tissue-resident memory T cells (TRMs) and central memory T cells (TCMs). …”
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Emergence and polyclonal dissemination of NDM-5/OXA-181 carbapenemase-producing Escherichia coli in the French Indian Ocean territories by Thibaut Vedani, Matthieu Pot, Thomas Garrigos, Loïk Sababadichetty, Marion Daniel, David Wilkinson, Thierry Benoit-Cattin, Olivier Belmonte, Patrick Mavingui, Laurent Dortet, Guillaume Miltgen

“…Conclusion We highlighted the dual dissemination of successful plasmids and the worrying circulation of high-risk clones via patients transfer between these two FOTs. …”
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CGH, cDNA and Tissue Microarray Analyses Implicate FGFR2 Amplification in a Small Subset of Breast Tumors by Mervi Heiskanen, Juha Kononen, Maarit Bärlund, Joachim Torhorst, Guido Sauter, Anne Kallioniemi, Olli Kallioniemi

“…High level amplification of FGFR2 in SUM‐52 was identified using CGH analysis on a microarray of BAC clones. A cDNA microarray survey of 588 genes showed >40‐fold overexpression of FGFR2. …”
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Mechanistic insights into 125I seed implantation therapy for Cholangiocarcinoma: focus on ROS-Mediated apoptosis and the role of GPX2 by Jun Luo, Zheng Yao, Weiren Liang, Danjun Song, Hui Zeng, Yi Jiang, Zhehan Bao, Jiaping Zheng, Yinan Ding

“…Apoptosis and proliferation were detected by TUNEL staining and clone formation, respectively. ROS generation was detected by dihydroethidium staining. …”
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Development and in vitro evaluation of biomimetic injectable hydrogels from decellularized human nerves for central nervous system regeneration by Gopal Agarwal, Kennedy Moes, Christine E. Schmidt

“…Human astrocytes, human microglial cell clone 3 (HMC3), and mouse RAW 264.7 macrophages were cultured individually within iHPN, with lipopolysaccharide (LPS) added to mimic CNS inflammation following injury. …”
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Importance of OsRac1 in Signalling of Pigm-1 Mediated Resistance to Rice Blast Disease by Dewei Yang, Niqing He, Fenghuang Huang, Jialin Chen, Minxiang Yu, Yidan Jin, Shaojun Lin, Shengping Li

“…The NLR protein Pigm-1 was cloned from Shuangkang 77009 in our laboratory. This study shows that the nucleotide-binding site (NBS) domain of Pigm-1 facilitates its binding to and activation of OsRac1 while the coiled-coil (CC) domain enables its binding to and activation of RAI1, ultimately inducing cell death. …”
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The concept of natural genome reconstruction. Part 1. Basic provisions of the “natural genome reconstruction” concept. Changing the genome of hematopoietic stem cells using several... by L. A. Yakubov, O. S. Taranov, S. V. Sidorov, S. D. Nikonov, A. A. Ostanin, E. R. Chernykh, N. A. Kolchanov, S. S. Bogachev

“…In this regard, there is a fundamental possibility of changing the hematopoietic status of hematopoietic stem cells in the direction of polyclonality and the original diversity of clones. Such events can form the basis for the rejuvenation of the blood-forming cell system. …”
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Construction and characterization of bioluminescent Salmonella Reading outbreak and non-outbreak strains by Abubakar Shitu Isah, Reshma Ramachandran, Anuraj Theradiyil Sukumaran, Aaron S. Kiess, Claudia D. Castañeda, Timothy Boltz, Kenneth S. Macklin, Hossam Abdelhamed, Li Zhang

“…The chloramphenicol acetyltransferase gene was cloned into the plasmid pBS-slpGFPluxABCDE, which carries luxABCDE operon and an ampicillin resistance gene. …”
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Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in <i>Lilium</i> ‘Siberia’ by Xiaoping Xu, Chenglong Yang, Yiping Zheng, Wenjie Guo, Zhongxiong Lai, Shaozhong Fang

“…<i>LoPurple acid phosphatase17</i> (<i>LoPAP17</i>), a pivotal gene of the riboflavin metabolism pathway, was subsequently cloned. LoPAP17 was most closely related to the orthologous genes of <i>Acorus calamus</i>, <i>Asparagus officinalis</i>, and <i>Musa acuminata</i>. …”
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