Functional evolution of mammalian odorant receptors. by Kaylin A Adipietro, Joel D Mainland, Hiroaki Matsunami

“…Using human and mouse ORs with previously identified ligands, we cloned 18 OR orthologs from chimpanzee and rhesus macaque and 17 mouse-rat orthologous pairs that are broadly representative of the OR repertoire. …”
Get full text

Determine the prevalence and fluoroquinolone resistance pattern of Escherichia coli ST131 among clinical isolates from vaginitis patients by Noor Ismeal Nasser, Mohammed Sabri Abdul-Razzaq, Nadia M. Al-Hili

“…Abstract Background: The most interesting lineage of Escherichia coli is the sequence type 131 (ST131), a pandemic clone that is responsible for the high incidence of E. coli infections outside the intestine; in addition, it contributes to the global dissemination of multiantibiotic resistance. …”
Get full text

Dynamic localization of glucokinase and its regulatory protein in hypothalamic tanycytes. by Magdiel Salgado, Estefanía Tarifeño-Saldivia, Patricio Ordenes, Carola Millán, María José Yañez, Paula Llanos, Marcos Villagra, Roberto Elizondo-Vega, Fernando Martínez, Francisco Nualart, Elena Uribe, María de Los Angeles García-Robles

“…Both GK and GKRP were next cloned from primary cultures of tanycytes. Expression of GK by Escherichia coli revealed a functional cooperative protein with a S0.5 of 10 mM. …”
Get full text

Une île impossible : l’utopie néo-humaine comme diagnostic du lien social contemporain chez Michel Houellebecq by Alice Bottarelli, Colin Pahlisch

“…Evolving (or rather stagnating) in a perpetual present, centred on a self which has lost all its solidity, detached from the possibility of having direct relationships with other individuals, the clones are becoming the victims of a form of virtualisation of all social links, a phenomenon described and criticised by Houellebecq as already being part of our present.…”
Get full text

Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells by Yazi Huang, Beilei Zhao, Yahan Liu, Nanping Wang

“…Genomic fragment containing 1.7-kilobase of the promoter region was cloned by using PCR. The luciferase reporter assays demonstrated that overexpression of PPARγ and rosiglitazone, a specific ligand for PPARγ, could significantly upregulate the reporter activity. …”
Get full text

TCR Gene Transfer: MAGE-C2/HLA-A2 and MAGE-A3/HLA-DP4 Epitopes as Melanoma-Specific Immune Targets by Trudy Straetemans, Mandy van Brakel, Sabine van Steenbergen, Marieke Broertjes, Joost Drexhage, Joost Hegmans, Bart N. Lambrecht, Cor Lamers, Pierre van Der Bruggen, Pierre G. Coulie, Reno Debets

“…We molecularly characterized TCRαβ genes of an MC2/A2-specific CD8 and MA3/DP4-specific CD4 T-cell clone derived from melanoma patients who responded clinically to MAGE vaccination. …”
Get full text

Potential of a novel and thermostable recombinant phage endolysin of Escherichia phage KW1E_UTAR against Shigella spp. by Selvam Dharshini, Mohd Ismail Nor Ismaliza, Ng Michelle Y. T.

“…In this study, the Escherichia phage KW1E_UTAR gene encoding for endolysin was successfully cloned and expressed in Escherichia coli pET-28a. …”
Get full text

No Correlation Exists between Disease Activity and the Expression of Killer-Cell Immunoglobulin-Like Receptors in Patients with Rheumatoid Arthritis by Toshiaki Kogure, Takeshi Tatsumi, Atsushi Niizawa, Hiroshi Fujinaga, Tomoyuki Ito, Yutaka Shimada, Katsutoshi Terasawa

“…The genes for killer-cell immunoglobulin-like receptors (KIRs) have been cloned and their functions and expression in patients with rheumatoid arthritis (RA) have been partially clarified. …”
Get full text

Feasibility of Ex Vivo Ligandomics by Prabuddha Waduge, Remya Ammassam Veettil, Bojun Zhang, Chengchi Huang, Hong Tian, Wei Li

“…However, in vivo ligandomics requires intracardial perfusion to remove unbound phage clones, limiting its use to vascular endothelial cells (ECs). …”
Get full text

Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases by Athanasios Mavropoulos, Dimitrios P. Bogdanos, Christos Liaskos, Timoklia Orfanidou, Theodora Simopoulou, Efterpi Zafiriou, Lazaros I. Sakkas, Eirini I. Rigopoulou

“…To overcome these limitations, we have modified methanol-based phosphoflow protocols using several commercially available antibody clones suitable for surface antigens, intracellular cytokines, and p38 MAPK. …”
Get full text

Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR by Martina Fazzina, Matteo Bergonzoni, Francesca Massenzio, Barbara Monti, Flavia Frabetti, Raffaella Casadei

“…Recently, the Human Microglial Clone 3 cell line (HMC3) has been characterized and a new human microglia model has emerged. …”
Get full text

The genomic copy number determination of improved Cre in transgenic mice using standards derived from megaprimer PCR constructed tandem repeats by Ge Diao, Min Tian, Runbo Li, Jie Huang, Jian Han, Jianxin Guo

“…Two rounds of megaprimer PCR were used to obtain specific tandem repeats of iCre fragments, which were subsequently cloned into pUCm-T vectors. After the fragments of IL-2 (reference gene) were ligated into the same vectors, standards with copy number ratios (CNR) of 0.5, 1, 2, 4, 8, and 16 between iCre and IL-2 were completed. …”
Get full text

Cyclic di AMP phosphodiesterase nanovaccine elicits protective immunity against Burkholderia cenocepacia infection in mice by Wesam E. Gawad, Yosra I. Nagy, Tamer M. Samir, Ahmed Mohamed Ibrahim Mansour, Omneya M. Helmy

“…Applying strict subtractive channels, three proteins were shortlisted: WP_006481710.1 (LY), WP_012493605.1 (KT), and WP_006492970.1 (BD). Proteins were cloned, purified as His-tagged proteins, and loaded onto C:P NPs. …”
Get full text

Cellular automata modelling of leukaemic stem cell dynamics in acute myeloid leukaemia: insights into predictive outcomes and targeted therapies by Yutaka Saikawa, Toshihiko Komatsuzaki, Nobuaki Nishiyama, Toshihisa Hatta

“…Our study revealed: (i) a correlation between LSC persistence post-induction chemotherapy and risk of AML relapse; (ii) MRD negativity based on LSC count may not reliably predict outcomes, supporting clinical evidence that patients with MRD-negative status can still be at risk of relapse; (iii) prolonged persistence of LSCs post-chemotherapy without disruption of normal haematopoiesis, aligning with clinical observations of dormant AML clones; (iv) early LSC dynamics post-induction chemotherapy, characterized by stochastic behaviours and movement velocities, are insufficient predictors of long-term prognosis; and (v) a distinct spatiotemporal organization of LSCs in later phases post-induction chemotherapy is correlated with long-term outcomes. …”
Get full text

The Microtubule-Associated Protein Tau and Its Relevance for Pancreatic Beta Cells by Magdalena Maj, Gregor Hoermann, Sazan Rasul, Wolfgang Base, Ludwig Wagner, Johannes Attems

“…We found upregulation of MAPT protein expression in human insulinomas when compared to human pancreatic islets of Langerhans and an imbalance between MAPT isoforms in insulinomas tissue. We cloned one 3-repeat domain MAPT and transduced this into a beta-cell derived rodent cell line Rin-5F. …”
Get full text

Plastid-localized ZmENR1/ZmHAD1 complex ensures maize pollen and anther development through regulating lipid and ROS metabolism by Shaowei Zhang, Xueli An, Yilin Jiang, Quancan Hou, Bin Ma, Qingping Jiang, Kai Zhang, Lina Zhao, Xiangyuan Wan

“…Using genetic mapping, we clone a key GMS gene, ZmENR1, which encodes a plastid-localized enoyl-acyl carrier protein (ACP) reductase. …”
Get full text

Expressional Subpopulation of Cancers Determined by G64, a Co-regulated Module by Jae-Woong Min, Sun Shim Choi

“…Studies of cancer heterogeneity have received considerable attention recently, because the presence or absence of resistant sub-clones may determine whether or not certain therapeutic treatments are effective. …”
Get full text

Postharvest Analysis of Lowland Transgenic Tomato Fruits Harboring hpRNAi-ACO1 Construct by Bita Behboodian, Zainon Mohd Ali, Ismanizan Ismail, Zamri Zainal

“…Initially, cDNA corresponding to ACO1 of lowland tomato cultivar (MT1), which has high identity with ACO1 of Solanum lycopersicum in GenBank, was cloned through RT-PCR. Using a partial coding region of ACO1, one hpRNAi transformation vector was constructed and expressed ectopically under the 35S promoter. …”
Get full text

Polymyalgia Rheumatica Revealing a Lymphoma: A Two-Case Report by Frank Verhoeven, Xavier Guillot, Mickaël Chouk, Clément Prati, Daniel Wendling

“…Blood exams highlighted the presence of a monoclonal B cell clone. Bone marrow concluded to a B cell lymphoma of the marginal zone. …”
Get full text

System Efficiency of a Tap Transformer Based Grid Connection Topology Applied on a Direct Driven Generator for Wind Power by Senad Apelfröjd, Sandra Eriksson

“…A full experimental set up of the system has been used in the laboratory study, where a clone of the on-site generator was driven by an induction motor and the system was connected to a resistive load to better evaluate the performance. …”
Get full text