Oral Plaque from Type 2 Diabetic Patients Reduces the Clonogenic Capacity of Dental Pulp-Derived Mesenchymal Stem Cells by Antonella Bordin, Francesca Pagano, Eleonora Scaccia, Matteo Saccucci, Iole Vozza, Noemi Incerti, Antonella Polimeni, Elena Cavarretta, Isotta Chimenti, Elena De Falco

“…Results have shown that the number of clones generated by DPSCs exposed to T2D oral plaque was significantly lower compared to controls (ctl). …”
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Multidrug-Resistant Salmonella enterica Serovar Rissen Clusters Detected in Azores Archipelago, Portugal by Leonor Silveira, Miguel Pinto, Joana Isidro, Ângela Pista, Patrícia Themudo, Luís Vieira, Jorge Machado, João Paulo Gomes

“…Considering the well-known spread of MDR between gastrointestinal bacteria, the identification of MDR circulating clones should constitute an alert to public health authorities. …”
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Assessment of different genotyping markers and algorithms for distinguishing Plasmodium falciparum recrudescence from reinfection in Uganda by Alex Mwesigwa, Monica Golumbeanu, Sam Jones, Sara L. Cantoreggi, Benson Musinguzi, Joaniter I. Nankabirwa, Everd Maniple Bikaitwoha, Joan N Kalyango, Charles Karamagi, Mateusz Plucinski, Samuel L. Nsobya, Christian Nsanzabana, Pauline Byakika-Kibwika

“…Microsatellites PfPK2 and Poly-α, potentially sensitive to detecting minority clones, are promising replacements for glurp. Discrepancies in recrudescence classification between match-counting and Bayesian algorithms highlight the need for standardized PCR correction practices.…”
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Placenta-derived mesenchymal stem cells protect against diabetic kidney disease by upregulating autophagy-mediated SIRT1/FOXO1 pathway by Honghong Liu, Jiao Wang, Guanru Yue, Jixiong Xu

“…P-MSCs improved HG-induced Mouse podocyte clone5(MPC5)injury, increased podocyte autophagy, and upregulated SIRT1 and FOXO1 expression. …”
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Analysis of expression and transport activity of sugar transporter gene HpSWEET7 from red pitaya by ZHENG Qianming, YAN Shuang, XIE Pu, WANG Honglin, MA Yuhua

“…[Objective] The red pitaya (Hylocereus polyrhizus) HpSWEET7 gene, belonging to the sugar transporter SWEET (sugars will eventually be exported transporter) family, was cloned and its role in fruit sugar accumulation was investigated, which provided a target gene for improving fruit flavor and quality. …”
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Real‐world efficacy of transfusion with liberal or restrictive strategy in traumatic brain injury by Liang‐Wen Cui, Nian Liu, Chao Yu, Ming Fang, Rui Huang, Cheng Zhang, Min Shao

“…Each eligible individual was cloned and assigned each of the replicates to one of the treatment arm. …”
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Transcription factor FOXD1 and miRNA-204-5p play a major role in B4GALNT2 downregulation in colon cancer by Martina Duca, Nadia Malagolini, Michela Pucci, Virginie Cogez, Anne Harduin-Lepers, Fabio Dall’Olio

“…Deletion experiments of the putative FOXD1 binding sites in the ~ 2800 bp sequence upstream of the B4GALNT2 transcriptional start site cloned in frame with the luciferase reporter gene, confirmed the regulatory role of FOXD1. …”
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Assessment of different genotyping markers and algorithms for distinguishing Plasmodium falciparum recrudescence from reinfection in Uganda. by Mwesigwa, Alex, Golumbeanu, Monica, Jones, Sam, Cantoreggi, L. Sara, Musinguzi, Benson, Nankabirwa, I. Joaniter, Bikaitwoha, Everd Maniple, Kalyango, N. Joan, Karamagi, Charles, Plucinski, Mateusz, Nsobya, L. Samuel, Nsanzabana, Christian, Byakika-Kibwika, Pauline

“…Microsatellites PfPK2 and Poly-α, potentially sensitive to detecting minority clones, are promising replacements for glurp. Discrepancies in recrudescence classification between match-counting and Bayesian algorithms highlight the need for standardized PCR correction practices…”
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The use of molecular-genetic and phytopathological methods to identify genes for effective leaf rust resistance in Aegilops accessions by L. G. Tyryshkin, M. A. Kolesova

“…In the phytopathological test, the seedlings were infected with the pathogen population (avirulent to Lr9 and Lr41 genes) and the fungus clones virulent to the wheat line with the Lr9 gene.Results and conclusions. …”
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Gene Polymorphism of Antigen B Subunit 2 and Pathogenesis of Cystic Echinococcosis in Murine Model by Hadi M. Alsakee, Hussein M. Abdulla, Reshna K. Albarzanji

“…Polymorphic sequencing profile of the PCR-amplified product (250 bp) of E. granulosus clone EgB2G2v13 AgB2 gene (Accession no.: AY569356.1) has no significant impact on the pathogenicity of the CE in murine model. …”
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Overcoming barriers for investigating nickel-pincer nucleotide cofactor-related enzymes by Jorge L. Nevarez, Aiko Turmo, Santhosh Gatreddi, Swati Gupta, Jian Hu, Robert P. Hausinger

“…Here, we show that when the Lactiplantibacillus plantarum lar genes are cloned into the Duet expression system and cultured in Escherichia coli, they confer lactate racemase activity to the cells. …”
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Light-chain proximal tubulopathy: a retrospective study from a single Chinese nephrology referral center by Xin Wang, Xiao-juan Yu, Su-xia Wang, Fu-de Zhou, Ming-hui Zhao

“…These patients showed improved or stable kidney function.Conclusions This is the first case series report of LCPT in four different pathological types in northern China. Clone-targeted chemotherapy may help preserve the kidney function in these patients.…”
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Features of the resource species Miscanthus sacchariflorus (Maxim.) Hack. when introduced in West Siberia by Yu. A. Gismatulina, V. V. Budaeva, G. V. Sakovich, O. Yu. Vasilyeva, G. A. Zueva, A. S. Gusar, O. V. Dorogina

“…Hence, it is preferable to cultivate seedlings on protected ground, and plantations are advisable to establish with more stable cloned vegetative material. The chemical compositions of the whole plant, leaf and stem separately, from seven M. sacchariflorus harvests were examined to reveal a rise in cellulose content and a drop in noncellulosic content with plantation age. …”
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Localization of rust resistance genes in old local Russian flaxes by methods of classical genetics by S. N. Kutuzova, E. A. Porokhovinova, N. B. Brutch, A. V. Pavlov

“…F2 hybrids in the phase  of cotyledon leaves were inoculated with monopustule clones  of the  fungus,  not  virulent  to any of evaluated genes.  …”
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Problems and prospects of grapevine genetic resources preservation at “Magarach” Institute by A. A. Polulyakh, V. A. Volynkin, V. V. Likhovsko

“…At the institute “Magarach” we have formed a vegetative collection of 40 grapevine varieties, hybrids and clones in vitro, and this work is under way. An effective way to solve the problem of gene pool preservation is cryopreservation, which is the main way of preserving genetic material of some crops. …”
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Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies by A. N. Chikaev, A. P. Rudometov, Yu. A. Merkulyeva, L. I. Karpenko

“…The biopanning procedure allows isolation of specific clones for almost any target, and due to the physical link between the genotype and the phenotype of recombinant phage particles it is possible to determine the structure of selected molecules. …”
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Interleukin-2/Anti-Interleukin-2 Immune Complex Expands Regulatory T Cells and Reduces Angiotensin II-Induced Aortic Stiffening by Beenish Majeed, Supannikar Tawinwung, Lance S. Eberson, Timothy W. Secomb, Nicolas Larmonier, Douglas F. Larson

“…To study the role of immune inhibitory cells, CD4+CD25+Foxp3+ regulatory T cells (Tregs), on vascular stiffness, we stimulated the proliferation of Treg lymphocytes in vivo using a novel cytokine immune complex of Interleukin-2 (IL-2) and anti-IL-2 monoclonal antibody clone JES6-1 (mAbCD25). Three-month-old male C57BL/6J mice were treated with IL-2/mAbCD25 concomitantly with continuous infusion of angiotensin type 1 receptor agonist, [Val5]angiotensin II. …”
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Design and expression of a chimeric recombinant antigen (SsIR-Ss1a) for the serodiagnosis of human strongyloidiasis: Evaluation of performance, sensitivity, and specificity. by Mostafa Omidian, Zohreh Mostafavi-Pour, Marzieh Asadi, Meysam Sharifdini, Navid Nezafat, Ali Pouryousef, Amir Savardashtaki, Mortaza Taheri-Anganeh, Fattaneh Mikaeili, Bahador Sarkari

“…The construct was expressed into the pET-23a (+) expression vector and the optimized DNA sequences of SsIR-Ss1a (873 bp) were cloned into competent E. coli DH5α cells. Diagnostic performances of the produced recombinant antigen, along with a commercial kit were evaluated in an indirect ELISA system, using a panel of sera from strongyloidiasis patients and controls. …”
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USING <i> AVENA MACROSTACHYA </i> FOR IMPROVEMENT OF OAT WINTERHARDINESS IN POLAND by B. . Lapinski, A. . Rachwalska

“…Large differences were noticed in fertility level and chromosome constitution of progeny of the intensely cloned and colchicine treated F1 hybrids. The B1F or F2 generation consisted of plants with chromosome numbers between 40 and 49, octoploids (2n=56) and plants carrying 60 to 70 chromosomes. …”
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The Function of Two <i>Brassica napus</i> β-Ketoacyl-CoA Synthases on the Fatty Acid Composition by Dongfang Zhao, Bingqian Zhou, Bo Hong, Jiajun Mao, Hu Chen, Junjie Wu, Li Liao, Chunyun Guan, Mei Guan

“…In <i>B. napus</i>, <i>BnaKCSA8</i> and <i>BnaKCSC3</i> belong to the KCS family, but their specific functions remain unclear. This study cloned <i>BnaKCSA8</i> and <i>BnaKCSC3</i> from <i>Brassica napus</i> L. and analyzed their functions. …”
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