The SEPALLATA-like gene HrSEP1 in Hippophae rhamnoides regulates flower development by interacting with other MADS-box subfamily genes by Di Cong, Xue Zhao, Xue Zhao, Chang Ni, Mengru Li, Luwen Han, Jianlin Cheng, Hongzhang Liu, Huijing Liu, Dan Yao, Shuying Liu, Guoshuang Chen

“…In this study, the cDNA sequence of the HrSEP1 gene was cloned by RT-PCR and confirmed that this gene belongs to the MADS-box gene family. …”
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Resistance to Multiple Tuber Diseases Expressed in Somaclonal Variants of the Potato Cultivar Russet Burbank by Tamilarasan Thangavel, Robert Steven Tegg, Calum Rae Wilson

“…Pot trials and in vitro assays with multiple pathogenic strains identified significant resistance to both tuber diseases across the potato variants examined; the best clone A380 showed 51% and 65% reductions in disease severity to tuber soft rot and black scurf, respectively, when compared with the parent line. …”
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PCR-Based Identification of Oral Streptococcal Species by Jeffrey A. Banas, Min Zhu, Deborah V. Dawson, Huojun Cao, Steven M. Levy

“…Sensitivity was 100% for selecting S. oralis and S. mitis among the clones sampled. Specificity was good except for the most closely related species that could not be reliably distinguished even by MLSA. …”
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A comparative study of replicative properties of antitumor recombinant vaccinia viruses on human glioblastoma cell culture U87 and monkey kidney cell culture CV-1 by R. A. Maksyutov, I. V. Kolosova, T. V. Tregubchak, I. A. Razumov, S. N. Shchelkunov

“…A promising basis for the creation of oncolytic drugs is orthopoxviruses, which have a number of advantages over other viral vectors, and one of these advantages is a large capacity of the genome, which allows genes encoding proteins with antitumor properties to be cloned into their genome. In this study, we compared the replicative properties of ten variants of vaccinia virus (the strain LIVP of VACV) using human glioblastoma cell culture; some of these viruses have additional genes, such as the gene encoding granulocyte-macrophage colony stimulating factor, gene encoding apoptosis-inducing protein TRAIL and gene encoding green fluorescent protein. …”
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A functional SNP of the core promoter region within goat CDC25A gene affects litter size by Taiyuan Zhang, Jingxuan Wang, Yangyang Bai, Qian Wang, Ke Wang, Haijing Zhu, Lei Qu, Zhengang Guo, Chuanying Pan, Xianyong Lan

“…In this study, five truncated fragments divided at position-2285 nt to +198 nt were amplified and cloned into the luciferase reporter vectors to identify the core promoter. …”
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Anthocyanin profiles and color properties of red wines made from Vitis davidii and Vitis vinifera grapes by Yanlun Ju, La Yang, Xiaofeng Yue, Yunkui Li, Rui He, Shenglin Deng, Xin Yang, Yulin Fang

“…Fifteen red spine grape clones and three red Vitis vinifera grape varieties were used to evaluate the differences in the anthocyanin profiles and color properties of wines made from V. davidii and V. vinifera grapes. …”
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Functional Characterization of the Canine Heme-Regulated eIF2α Kinase: Regulation of Protein Synthesis by Kimon C. Kanelakis, Jayashree Pyati, Pamela C. Wagaman, Jui Chang Chuang, Young Yang, Nigel P. Shankley

“…In this work, we have cloned and expressed the canine form of the HRI kinase. …”
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Humanin Rescues Cultured Rat Cortical Neurons from NMDA-Induced Toxicity Not by NMDA Receptor by Ai-Ling Cui, Jian-Zhong Li, Zhi-Bo Feng, Guo-Lin Ma, Liang Gong, Chun-Ling Li, Ce Zhang, Kefeng Li

“…Humanin is a 24-aa peptide cloned from the brain of patients with Alzheimer’s disease (AD). …”
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Subtilisin-like protease 4 regulates cold tolerance through cell wall modification in rice by Jingyan Liu, Fei He, Zhicai Chen, Meng Liu, Yingni Xiao, Ying Wang, YuMeng Cai, Jin Du, Weiwei Jin, Xuejun Liu

“…Despite extensive research into the cold tolerance mechanisms of rice, the number of cloned genes remains limited. Plant subtilisin-like proteases (SUBs or SBTs) are protein-hydrolyzing enzymes which play important roles in various aspects of plant growth as well as the plant response to biotic and abiotic stress. …”
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A SH3b domain-containing peptidoglycan recognition protein from Magallana gigas with broad recognition spectrum by Yinan Li, Hongbo Lu, Chuanyan Yang, Zhuyun Liu, Weilin Wang, Chang Liu, Lingling Wang

“…In the present study, nine MgPGRP genes were identified in the updated genome of Magallana gigas, and the cDNA of MgPGRP5 which was consisted of a SH3b and a PGRP domain was cloned. The mRNA transcripts of MgPGRP5 were constitutively expressed in haemocytes and all the examined tissues, with the highest expression level in hepatopancreas. …”
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MADS-box encoding gene Tunicate1 positively controls maize yield by increasing leaf number above the ear by Yan Li, Jian Wang, Shuyang Zhong, Qiang Huo, Qun Wang, Yunlu Shi, Hangqin Liu, Jiacheng Liu, Yang Song, Xiaojian Fang, Zhongwei Lin

“…However, increasing the number of leaves above the ear to strengthen the source and improve maize yield remains challenging in modern maize breeding. Here, we clone the causative gene of the quantitative trait locus (QTL) associated with the number of leaves above the ear. …”
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Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals by Jinhui Cui, Haixin Cui, Yan Wang, Changjiao Sun, Kui Li, Hongyan Ren, Wei Du

“…This work provides important experimental basis for the application of the magnetic nanoparticles on gene delivery to porcine somatic cells, which is significant for the achieving of breeding new transgenic cloned pigs by using somatic cell nuclear transfer technique.…”
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A CRISPR-Cas9 system protecting E. coli against acquisition of antibiotic resistance genes by Danna Lee, Petra Muir, Sara Lundberg, August Lundholm, Linus Sandegren, Sanna Koskiniemi

“…We synthesized a CRISPR locus targeting eight AMR genes and cloned this with the Cas9 and transacting tracrRNA on a medium copy plasmid. …”
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Characterization of an allotriploid strawberry Fragaria × bifera Duchesne (Rosaceae) from Europe by Houshang Nosrati, Adam H. Price, Pedro Gerstberger, Chris C. Wilcock

“…To assess its role in speciation, it is necessary to examine fertility and crossability of hybrids. A hybrid clone of the genus Fragaria with different and complex morphology compared to F. vesca, F. viridis and F. moschata, was detected in Germany (in Bayreuth, Bavaria). …”
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INTERGENOMIC CHROMOSOME PAIRING IN ALLOHAPLOID HYBRIDS OF GENUS SOLANUM by T. A. Gavrilenko, G. I. Pendinen, V.-M. Rokka, O. Y. Antonova, R. Thieme

“…Genome and chromosome composition of allohaploids were identified through FISH with chromosome specific BAC clones and GISH. Up to 7 intergenomic bivalents per cell were observed in allohaploids between S. tuberosum and S. etuberosum (AE); the chiasmata were distributed in the distal regions of long arms of each chromosome and in the short arms of chromosomes 3, 6, 11, 12. …”
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Effect of Planting Material Type on Experimental Trial Quality and Performance Ranking of Sugarcane Genotypes by Michel Choairy de Moraes, Ana Carolina Ribeiro Guimarães, Dilermando Perecin, Manuel Benito Sainz

“…However, the effect of planting material type on the performance ranking and consequent selection of sugarcane clones in a breeding program has not been previously investigated. …”
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Isolation, identification, and characterisation of the malachite green detoxifying bacterial strain Bacillus pacificus ROC1 and the azoreductase AzrC by Shanza Bibi, Callum W. Breeze, Vusqa Jadoon, Anum Fareed, Alina Syed, Rebecca L. Frkic, Habiba Zaffar, Muhammad Ali, Iftikhar Zeb, Colin J. Jackson, Tatheer Alam Naqvi

“…Genomic sequencing identified three candidate azo-reductases within B. pacificus ROC1 that could be responsible for the MG-degrading activity. These were cloned, expressed and purified from Escherichia coli, with one (AzrC), catalyzing the reduction of MG to leuco-MG in vitro. …”
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PPARγ Regulates Genes Involved in Triacylglycerol Synthesis and Secretion in Mammary Gland Epithelial Cells of Dairy Goats by Hengbo Shi, Jun Luo, Jiangjiang Zhu, Jun Li, Yuting Sun, Xianzi Lin, Liping Zhang, Dawei Yao, Huaiping Shi

“…To explore the function of PPARγ in the goat mammary gland, we cloned the whole cDNA of the PPARγ gene. Homology alignments revealed that the goat PPARγ gene is conserved among goat, bovine, mouse, and human. …”
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Eucalyptus and Water Use in South Africa by Janine M. Albaugh, Peter J. Dye, John S. King

“…Greater insights into the water use efficiency of clonal material are needed, as certain eucalypt clones show fast growth and low water use. To better understand water use efficiency, estimates should be combined with monitoring of stand canopy structure and measurements of physiological processes.…”
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Functional Development of the Human Gastrointestinal Tract: Hormone- and Growth Factor-Mediated Regulatory Mechanisms by Daniel Ménard

“…These models comprise a novel primary culture system of human fetal gastric epithelium including, for the first time, functional chief cells, and human gastric epithelium cell lines cloned from the parental NCI-N87 strain. These new cells lines could serve important applications in the study of pathogenic action and epithelial regeneration.…”
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