Heterologous expression of SpsTAC2 in Arabidopsis affected branch angle and secondary vascular system development by Fangshu Niu, Mengru Yuan, Hongxia Zhao, Zhi Pang, Jie Yan, RuiXie Ning, Lin Shi, Fengqiang Yu, Dongshan Wei, Rong Yang, Runming Zhang, Haifeng Yang

“…In this study, TAC2 was cloned from Salix psammophila, and an overexpression and subcellular localization expression vector for the SpsTAC2 gene was constructed. …”
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Multi-omics analysis reveals that neutrophil extracellular traps related gene TIMP1 promotes CRC progression and influences ferroptosis by Yuzhao Jin, Luyu Liao, Qianping Chen, Bufu Tang, Jin Jiang, Ji Zhu, Minghua Bai

“…We used small interfering RNA to knockdown TIMP1, and verified the ability of TIMP1 in CRC proliferation, invasion and migration through western blot, transwell, cell scratch assay, cell clone formation and other experiments. Result We screened out three major NETs Genes: TIMP1, F3, and CRISPLD2 based on machine learning. …”
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The Progression of Mycosis Fungoides During Treatment with Mogamulizumab: A BIO-MUSE Case Study of the Tumor and Immune Response in Peripheral Blood and Tissue by Angelica Johansson, Eirini Kalliara, Emma Belfrage, Teodor Alling, Paul Theodor Pyl, Anna Sandström Gerdtsson, Urban Gullberg, Anna Porwit, Kristina Drott, Sara Ek

“…<b>Conclusions:</b> We propose that in patients with MF, the evolution of the malignant clone and the associated immune response need to be studied jointly to define relevant strategies for intervention.…”
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Single cell transcriptome profiling reveals pathogenesis of bullous pemphigoid by Guirong Liang, Chenjing Zhao, Qin Wei, Suying Feng, Yetao Wang

“…Blood CX3CR1+ ZNF683+ and LAG3+ exhausted T cells exhibit the highest TCR expansion among clones shared with skin CD8+T cells, suggesting their role in fueling skin CD8+T cell clonal expansion. …”
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A Novel PET Imaging Using 64Cu-Labeled Monoclonal Antibody against Mesothelin Commonly Expressed on Cancer Cells by Kazuko Kobayashi, Takanori Sasaki, Fumiaki Takenaka, Hiromasa Yakushiji, Yoshihiro Fujii, Yoshiro Kishi, Shoichi Kita, Lianhua Shen, Hiromi Kumon, Eiji Matsuura

“…Mesothelin (MSLN) is a 40-kDa cell differentiation-associated glycoprotein appearing with carcinogenesis and is highly expressed in many human cancers, including the majority of pancreatic adenocarcinomas, ovarian cancers, and mesotheliomas, while its expression in normal tissue is limited to mesothelial cells lining the pleura, pericardium, and peritoneum. Clone 11-25 is a murine hybridoma secreting monoclonal antibody (mAb) against human MSLN. …”
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Genomic Characterization of <i>Laodelphax striatellus</i> Permutotetra-like Virus and Self-Cleavage Function of Viral Capsid Protein by Jun Piao, Jiarui Zhang, Lujie Zhang, Jingai Piao, Haitao Wang, Yilin Xie, Shuo Li

“…In this paper, the full-length genome of LsPLV was cloned and analyzed, then viral capsid protein (CP) was expressed and prepared as an antibody, and CP property was tested. …”
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Identification and Characterization of the Diverse Stress-Responsive R2R3-RMYB Transcription Factor from Hibiscus sabdariffa L. by Bahaeldeen Babikar Mohamed, Beenish Aftab, Muhammad Bilal Sarwar, Bushra Rashid, Zarnab Ahmad, Sameera Hassan, Tayyab Husnain

“…Furthermore, RMYB gene was cloned into plant expression vector under CaMV35S promoter and transformed to Gossypium hirsutum: a local cotton cultivar. …”
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A Nonsynonymous Single-nucleotide Polymorphism in SLC24A5 Regulates Feather Pigment Deposition in Chinese Yellow Quail by Xiaohui Zhang, Fanghu Wu, Yanxia Qi, Yuanyuan Shang, Lingyun Fan, Yifei Wang

“…The cDNA of the SLC24A5 gene was cloned by RT-PCR and subjected to Sanger sequencing. …”
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Dorsal Root Ganglion Maintains Stemness of Bone Marrow Mesenchymal Stem Cells by Enhancing Autophagy through the AMPK/mTOR Pathway in a Coculture System by Shuaishuai Zhang, Junqin Li, Huijie Jiang, Yi Gao, Pengzhen Cheng, Tianqing Cao, Donglin Li, Jimeng Wang, Yue Song, Bin Liu, Hao Wu, Chunmei Wang, Liu Yang, Guoxian Pei

“…Crystal violet staining showed that the clone-forming ability of BMSCs in the coculture group was also enhanced and mRNA levels of Sox2, Nanog, and Oct4 were significantly upregulated in the coculture group. …”
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Anther transcriptomes in cold-tolerant rice cultivars tend to show insensitive responses by Koichi Yamamori, Seiya Ishiguro, Kei Ogasawara, Kayyis Muayadah Lubba, Kaien Fujino, Kazumitsu Onishi, Yutaka Sato, Yuji Kishima

“…Many studies of stress tolerance in plants have characterized genes that show differences among a small number of lines with clearly distinct tolerance or sensitivity to the given stress. From the few cloned genes, it is difficult to genetically interpret intermediate tolerance or susceptibility levels and explain the complexity of stress responses and tolerance. …”
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Clonality and the Phenotype–Genotype Correlation of Antimicrobial Resistance in <i>Acinetobacter baumannii</i> Isolates: A Multicenter Study of Clinical Isolates from Romania by Adrian-Gabriel Pană, Pavel Șchiopu, Dan Alexandru Țoc, Vlad Sever Neculicioiu, Anca Butiuc-Keul, Anca Farkas, Matei-Ștefan Dobrescu, Mirela Flonta, Carmen Costache, Izabella Éva Szász, Lia-Monica Junie

“…The isolates, although nearly identical in phenotype, displayed different genotypical profiles, with varying degrees of similarity across hospitals and cities, raising the possibility of both local outbreaks of a single clone and widespread dissemination of resistant isolates.…”
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Atypical Metastases from Prostate Cancer: Alpha-Methylacyl-Coenzyme A Racemase (AMACR) as a Potential Molecular Target in Prostate-Specific Membrane Antigen-Negative Prostate Adeno... by Ilham Badrane, Angelo Castello, Matteo Brunelli, Corrado Cittanti, Sara Adamantiadis, Ilaria Bagni, Noemi Mindicini, Federica Lancia, Massimo Castellani, Licia Uccelli, Mirco Bartolomei, Luca Urso

“…Moreover, 2-deoxy-2-[18F]fluoro-D-glucose ¹⁸F-FDG PET usually shows metastases in the late stages of disease, when dedifferentiated neoplastic clones lose PSMA expression. In some cases, PCa patients may present atypical sites of metastases, with uncommon appearance at PET imaging with different radiotracers. …”
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Expression of Interferon Effector Gene SART1 Correlates with Interferon Treatment Response against Hepatitis B Infection by Yong Li, Chuanlong Zhu, Faxi Wang, Tiantian Zhu, Jun Li, Shufeng Liu, Fei Xiao

“…In this study, we investigated the role of SART1 in antiviral activity of IFN-α against hepatitis B virus (HBV) using blood and liver biopsy samples from chronic hepatitis B patients treated with pegylated IFN-α and HepG2 cells transfected with cloned HBV DNA. We observed that the basal SART1 expression in liver and PBMCs before IFN treatment was significantly higher in responders than in nonresponders. …”
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Cryo-thermal therapy reshaped the tumor immune microenvironment to enhance the efficacy of adoptive T cell therapy by Shicheng Wang, Peng Peng, Junjun Wang, Zelu Zhang, Ping Liu, Lisa X. Xu

“…The remodeling of the tumor immune microenvironment further enhanced the antitumor efficiency of ACT by increasing the proliferation of T cells, promoting activation of the effector functions of T cells and boosting the expansion of TCR clones. Conclusions Our results suggest that CTT can significantly reshape the tumor immunosuppressive microenvironment and convert “cold tumors” into “hot tumors,” thereby enhancing ACT-induced immune responses and maximizing the therapeutic effect of ACT.…”
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Peripheral T-Cell Lymphoma with Aberrant Expression of CD19, CD20, and CD79a: Case Report and Literature Review by Rahul G. Matnani, Rachel L. Stewart, Joseph Pulliam, Chester D. Jennings, Melissa Kesler

“…The current report also highlights the need for molecular gene rearrangement studies to determine the precise lineage of ambiguous neoplastic clones.…”
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Unraveling the degradation mechanism of multiple pyrethroid insecticides by Pseudomonas aeruginosa and its environmental bioremediation potential by Hui Liu, Wen-Juan Chen, Zeling Xu, Shao-Fang Chen, Haoran Song, Yaohua Huang, Kalpana Bhatt, Sandhya Mishra, Mohamed A. Ghorab, Lian-Hui Zhang, Shaohua Chen

“…A new esterase gene (estA) containing conserved motif (GDSL) and catalytic triad (Ser38, Asp310 and His313) was cloned from strain PAO1. Enzyme activity and gene knockout experiments confirmed the pivotal role of estA in pyrethroid biodegradation. …”
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Heterologous Expression of Laccase1 from Cryphonectria parasitica in Saccharomyces cerevisiae by Kum-Kang So, Fatima Alejandra Hernandez Alvarado, Gui-Hwan Han, Jeong-Won Kim, Tae-Geum Kim, Dae-Hyuk Kim

“…To obtain high and stable expression of fungal laccases, we cloned the gene encoding an extracellular laccase (Laccase1) of C. parasitica into a yeast episomal vector, used the resulting vectors to transform S. cerevisiae, and optimized the culture conditions of the selected transformants for Laccase1 production. …”
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Field apple scab susceptibility of a diverse Malus germplasm collection identifies potential sources of resistance for apple breeding by David Papp, Liqiang Gao, Ranjita Thapa, Dan Olmstead, Awais Khan

“…Cultivars carrying the Rvi6 (Vf) gene from Malus floribunda clone 821, e.g. ‘Liberty’ or ‘Florina’, remained resistant despite the breakdown of Rvi6. …”
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Genetic Analysis of Cytokine Promoters in Nonhuman Primates: Implications for Th1/Th2 Profile Characteristics and SIV Disease Pathogenesis by Pavel Bostik, Melanie Watkins, Francois Villinger, Aftab A. Ansari

“…To address this issue promoter regions for the major Th1/Th2 cytokines from RM and SM were cloned and sequenced. Sequence analysis of promoter fragments of IL-4, IL-10, IL-12 p40, IFN-gamma and TNF-alpha from the two monkey species showed varying degree of homology ranging from high degree of homology detected for IFN-gamma promoter (>99%) to relatively high degree of polymorphism detected for TNF-alpha promoter (94% homology). …”
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An analytical “quality by design” approach in RP-HPLC method development and validation for reliable and rapid estimation of irinotecan in an injectable formulation by Bhaskaran Navya Ajitkumar, Kumar Lalit, Reddy M Sreenivasa, Pai Girish K

“…RP-HPLC method was developed by applying Box-Behnken design with Hyper-Clone (Phenomenex®) C18 column (250 × 4.6 mm id, particle size 5 µm, ODS 130 Å) as a stationary phase. …”
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