Amphetamine Positive Urine Toxicology Screen Secondary to Atomoxetine by Joshua L. Fenderson, Amy N. Stratton, Jennifer S. Domingo, Gerald O. Matthews, Christopher D. Tan

“…On arrival to the hospital, a urine toxicological drug screen with immunochemical cloned enzyme donor immunoassay (CEDIA) was performed. …”
Get full text

Bioaccessibility In Vitro of Nutraceuticals from Bark of Selected Salix Species by Urszula Gawlik-Dziki, Danuta Sugier, Dariusz Dziki, Piotr Sugier

“…The aim of this study was to investigate and to compare the extractability, bioaccessibility, and bioavailability in vitro of antioxidative compounds from bark of selected Salix species: S. alba (SA), S. daphnoides (SD), S. purpurea (SP), and S. daphnoides x purpurea (SDP) hybrid willow clones originating from their natural habitats and cultivated on the sandy soil. …”
Get full text

Stromal-Cell-Derived Factor-1 (SDF-1)/CXCL12 as Potential Target of Therapeutic Angiogenesis in Critical Leg Ischaemia by Teik K. Ho, X. Shiwen, D. Abraham, J. Tsui, D. Baker

“…More than 50 different chemokines and 20 different chemokine receptors have been cloned. The chemokine stromal-cell-derived factor-1 (SDF-1 aka CXCL12) is a constitutively expressed and inducible chemokine that regulates multiple physiological processes, including embryonic development and organ homeostasis. …”
Get full text

Leveraging AI to automate detection and quantification of extrachromosomal DNA to decode drug responses by Kohen Goble, Aarav Mehta, Damien Guilbaud, Jacob Fessler, Jingting Chen, William Nenad, William Nenad, Christina G. Ford, Oliver Cope, Darby Cheng, William Dennis, Nithya Gurumurthy, Yue Wang, Kriti Shukla, Elizabeth Brunk, Elizabeth Brunk, Elizabeth Brunk, Elizabeth Brunk, Elizabeth Brunk

“…However, cancer cells also utilize DNA-based strategies, often perceived as slow, irreversible changes like point mutations or drug-resistant clone selection. Extrachromosomal DNA (ecDNA), in contrast, represents a rapid, reversible, and predictable DNA alteration critical for cancer’s adaptive response.MethodsIn this study, we developed a novel post-processing pipeline for automated detection and quantification of ecDNA in metaphase Fluorescence in situ Hybridization (FISH) images, leveraging the Microscopy Image Analyzer (MIA) tool. …”
Get full text

Molecular characterization of ST15 carbapenem-resistant Klebsiella pneumoniae isolated in a single patient by Yongjin Hu, Rong Tang, Shanshan Jin, Jiahao Guan, Xiaoxiao Meng, Zengpeijie Dan, Ruilan Wang, Hong-Yu Ou, Jian Lu

“…The plasmid p2-KP3762–1, harbouring the carbapenem resistance gene blaKPC-2, may undergo inter-strain and inter-clone transfer via conjugation in the patient during treatment. …”
Get full text

Evaluation of the double-zone hemolysis (DZH) test for the detection of livestock-associated methicillin-resistant Staphylococcus aureus by Javier Latorre-Fernández, Carmen Aspiroz, Idris Nasir Abdullahi, Allelen Campaña-Burguet, Paula Eguizábal, Carmen González-Azcona, Carmen Tenorio, Myriam Zarazaga, Adebayo O. Shittu, Carmen Lozano, Carmen Torres

“…The proposed double-zone hemolysis test has shown high positive and negative predictive values, sensitivity, and specificity to detect these LA-MRSA clones and S. aureus of potential animal origin. Most LA-MRSA clones exhibit resistance to different classes of antibiotics, with unique epidemiological characteristics, and their early detection has public health relevance and patient management.…”
Get full text

Arginase-1-specific T cells target and modulate tumor-associated macrophages by Mads Hald Andersen, Inés Lecoq, Ayako Wakatsuki Pedersen, Evelina Martinenaite, Shamaila Munir Ahmad, Maria Perez-Penco, Lucia Lara de la Torre, Marion Chapellier, Lars Rønn Olsen, Mia Aaboe-Jørgensen, Hannah Jorinde Glöckner, Anne Mette Askehøj Rømer

“…Human Arg1-specific CD4+T cell clones were co-cultured with Arg1-expressing TAMs generated in vitro from either PBMC-derived CD14+cells or the myeloid cell lines MonoMac1 and THP-1. …”
Get full text

Treatment of tumour tissue with radio‐frequency hyperthermia (using antibody‐carrying nanoparticles) by Reza Didarian, Ibrahim Vargel

“…After that, a monoclonal antibody (Monoclonal Anti‐N‐CAM Clone NCAM‐OB11) was immobilised by the 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide/N‐hydroxysuccinimide method. …”
Get full text

Circulación de saberes y apropiación del conocimiento local alrededor del cultivo de cacao en Talamanca, Costa Rica by Tania Rodríguez Echavarría

“…Talamanca se constituyó así en un laboratorio de experimentación de clones y estrategias productivas que buscaban cambiar el modo de producción indígena para hacerlo más competitivo siguiendo la lógica del mercado.     …”
Get full text

Distribution and genetic diversity of three apple viruses in Belarus by P. V. Kuzmitskaya, O. Yu. Urbanovich

“…The genome fragments of the ACLSV, ASPV, and ASGV viruses detected were cloned and sequenced. The analysis of their nucleotide sequences gives evidence of high molecular variability generated mostly by single nucleotide substitutions and rarely by single nucleotide deletions and insertions. …”
Get full text

Changes in the Physiological Parameters of SbPIP1-Transformed Wheat Plants under Salt Stress by G. H. Yu, X. Zhang, H. X. Ma

“…The SbPIP1 gene is a new member of the plasma membrane major intrinsic gene family cloned from the euhalophyte Salicornia bigelovii Torr. …”
Get full text

Development of Multiple Myeloma of the IgA Type in a Patient with Cold Agglutinin Disease: Transformation or Coincidence? by Øystein Sefland, Ulla Randen, Sigbjørn Berentsen

“…If a monoclonal immunoglobulin other than IgMκ is found in a patient with CAD, the coexistence of two unrelated B-cell clones should be suspected.…”
Get full text

Heterologous Expression and Transglycosylation of Acarviosyltransferase by XUE Zhenglian, WANG Yuqing, LI Chuang, LI Dandan, ZHU Sibao, LI Xiangfei

“…In order to further investigate the structural properties and catalytic function of acarviosyltransferase (ATase), a key enzyme in the biosynthesis of acarbose, its gene (acbD) was cloned from Actinoplanes sp. SE50 genome and heterologously expressed in Escherichia coli. …”
Get full text

The Hematopoietic Niche in Myeloproliferative Neoplasms by Annette H. Schmitt-Graeff, Roland Nitschke, Robert Zeiser

“…Malignant HSCs may alter the function and survival of MSCs that do not belong to the neoplastic clone. For example, a regression of nestin+ MSCs by apoptosis has been attributed to neuroglial damage in MPN. …”
Get full text

Perfil sensorial de cuatro modelos de siembra de cacao en Colombia by Lucas Fernando Quintana Fuentes, Alberto García Jerez, Edith Moreno Martínez

“…Los modelos evaluados corresponden a los siguientes clones; Ml: Testigo CCN-51, M2: Materiales con tamaño de grano grande FSV 41 - ICS 39 - ICS - ICS 1, M3: Materiales de alta producción FSA 13 - ICS 1 - FLE 3 - TSH 565, M4: Materiales con resistencia Mo-nilia FEC 2 - CAUC 39 - ICS 95 - FLE 2. …”
Get full text

Protracted Clonal Trajectory of a JAK2 V617F-Positive Myeloproliferative Neoplasm Developing during Long-Term Remission from Acute Myeloid Leukemia by Stephen E. Langabeer, Karl Haslam, Maria Anne Smyth, John Quinn, Philip T. Murphy

“…This putative chemoresistant clone subsequently expanded over the intervening seven years, resulting in a hematologically overt MPN. …”
Get full text

Epidemiological Analysis of a <i>K. pneumoniae</i> NDM Outbreak in a Temporary Ward for Patients with Primary COVID-19 Infection by Agata Pruss, Klaudia Miładowska, Helena Masiuk, Paweł Kwiatkowski, Joanna Jursa-Kulesza, Bartosz Wojciuk, Stefania Giedrys-Kalemba, Barbara Dołęgowska

“…We report a hospital outbreak due to New Delhi carbapenemases producing <i>K. pneumoniae</i> clones. Twenty-eight <i>K. pneumoniae</i> strains were analyzed from patients with primary COVID-19 infection. …”
Get full text

Killer-Cell Inhibitory Receptors, CD158a/b, are Upregulated by Interleukin-2, but not Interferon-γ or Interleukin-4 by Toshiaki Kogure, Hiroshi Fujinaga, Atsushi Niizawa, Le Xuan Hai, Yutaka Shimada, Hiroshi Ochiai, Katsutoshi Terasawa

“…Although it is now accepted that killer-cell inhibitory receptors (KIRs), which were molecularly cloned in 1995, deliver negative signals to natural killer (NK) cells regarding the recognition of target cells, it is still unclear how the expression of these receptors on lymphocytes is regulated. …”
Get full text

Systematic functional analysis and potential application of a serine protease from cold-adapted Planococcus bacterium by Weijun Leng, Xiaoyun Wu, Xianghui Qi, Hongying Liu, Li Yuan, Ruichang Gao

“…In this study, a gene encoding serine protease (PmSpr288) from cold-adapted bacterium, namely Planococcus maritimus XJ11, was cloned and overexpressed in Escherichia coli BL21 (DE3). …”
Get full text

A root system architecture regulator modulates OsPIN2 polar localization in rice by Yong Li, Meiyan Ren, Yunrong Wu, Lingling Wang, Keju Zhao, Hongsheng Gao, Mengzhen Li, Yu Liu, Jianshu Zhu, Jiming Xu, Xiaorong Mo, Zhongchang Wu, Chungui Lu, Shaojian Zheng, Chuanzao Mao

“…Abstract Ideal root system architecture (RSA) is important for efficient nutrient uptake and high yield in crops. We cloned and characterized a key RSA regulatory gene, GRAVITROPISM LOSS 1 (OsGLS1), in rice (Oryza sativa L.). …”
Get full text