Are Ancient Durum Wheats Less Toxic to Celiac Patients? A Study of α-Gliadin from Graziella Ra and Kamut by M. Stella Colomba, Armando Gregorini

“…For all accessions, a few α-gliadin alleles were also cloned, sequenced and translated into aminoacid sequences. …”
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Effect of Codon Optimisation on the Production of Recombinant Fish Growth Hormone in Pichia pastoris by Hussin A. Rothan, Teh Ser Huy, Zulqarnain Mohamed

“…The optimized FGH coding sequence (oFGH) and native sequence (nFGH) of giant grouper fish (Epinephelus lanceolatus) were cloned into P. pastoris expression vector (pPICZαA) downstream of alcohol oxidase gene (AOX1) for efficient induction of extracellular rFGH by adding 1% of absolute methanol. …”
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<i>Ex situ</i> conservation of <i>Rubus</i> L. (Rosaceae) genetic resources (a review) by S. E. Dunaeva, L. S. Krasovskaya, T. A. Gavrilenko

“…Wild species are mainly represented by those collected in the Russian North – 49 clones of red raspberry (Rubus idaeus L.) and 6 of cloudberries (R. chamaemorus L.) – and in the Caucasus (35 accessions of 26 species). …”
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Intratumor heterogeneity: models of malignancy emergence and evolution by R. A. Ivanov, S. A. Lashin

“…This knowledge enables the development of innovative strategies for targeting specific tumor clones, minimizing the risk of recurrence and improving patient outcomes. …”
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Inotodiol induces hepatocellular carcinoma apoptosis by activation of MAPK/ERK pathway. by Yushuang Xing, Di Jia, Xinping Zhu, Jialu Yang, Zhipeng Gao, Nana Meng, Haohao Xu, Mengxiao Wang, Shijun Chang, Mingqian Zhao, Shanbo Zhang, Zichen Mu, Qiang Tang, Weiming Zhao

“…The CCK8 assay was utilized to assess cell viability, the scratch assay was utilized to detect migration ability, the clone formation assay was utilized to detect clonogenic ability, and flow cytometry was utilized to analyze apoptosis and cell cycle. …”
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Status of the First Permanent Molars in Students from the José Antonio Saco Elementary School by Diosky Ferrer Vílchez, Ana Belkys Hernández Millán, Disney Tablada Peralta, Ana Ivis Betancourt García, Antonio Araujo Vílchez, Yenly Rodríguez Alonso

“…<br /><strong>Conclusion:</strong> the status of the first permanent molars can be generally classified as favorable, which was demonstrated through Clone’s index. A history of dental caries was a predisposing risk factor for the development of this condition; poor hygiene, presence of deep grooves and parental ignorance were also significant.…”
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Expression evaluation of exogenous and endogenous alcohol dehydrogenase genes in transgenic Arabidopsis by Haoqiang Yu, Hong Lv, Fengzhong Lu, Qingqing Yang, Huaming Duan, Wanchen Li, Fengling Fu

“…To detect the differences in expression, protein accumulation, and enzyme activity between transformed exogenous and endogenous genes, the coding sequences (CDSs) of the alcohol dehydrogenase (ADH) genes were cloned from dicotyledonous Arabidopsis, monocotyledonous maize, and prokaryotic Escherichia coli, constructed into expression vector pBI121-cMycNY, and used to transform wild-type Arabidopsis, respectively. …”
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Genetic control of scald resistance in barley landraces by O. N. Soboleva, G. S. Konovalova, E. E. Radchenko

“…Analysis of the interaction between the pathogen test clones and the host plant revealed a difference between the alleles determining fungal resistance in 32 barley forms, the previously effective genes Rrs4, rrs6, rrs7, and currently effective Rrs9. …”
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Investigating the Existence of Ribosomal Protein L5 Gene in Syrian Strain of Leishmania tropica Genome: Sequencing It and Evaluating Its Immune Response as DNA Vaccine by Mohammad Maarouf, Alyaa A. Abdlwahab

“…After proving the existence of the ribosomal protein L5 gene in a Syrian strain of Leishmania tropica (LCED Syrian 01), it was sequenced and cloned into a pCI plasmid, and the designed DNA vaccine was administered to BALB/c mice. …”
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The <i>Pyrus sinkiangensis Yu PsLEA4</i> Gene Enhances the Cold Resistance of <i>Solanum lycopersicum</i> by Xueying Yang, Wenjuan Zhao, Hui Li, Zhenxia Zhao, Jianbo Zhu, Jin Li

“…In order to evaluate the biological function of the <i>PsLEA4</i> protein under low-temperature stress and its potential for use in agricultural breeding, we cloned the <i>PsLEA4</i> gene from the Korla Pear, made a plant overexpression vector, and transformed it into a tomato via Agrobacterium transformation. …”
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Isolation, Characterization, and Molecular Modeling of a Rheumatoid Factor from a Hepatitis C Virus Infected Patient with Sjögren’s Syndrome by Yu-Ching Lee, Keng-Chang Tsai, Sy-Jye Leu, Tuan-Jen Wang, Chia-Yu Liu, Yi-Yuan Yang

“…After panning, a specific clone RFL11 was isolated for characterization in advance. …”
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Genetic assessment in early clonal population in sugarcane (Saccharum officinarum L.) for productivity traits and resistance to foliar diseases by B. Shivarudra1, Sanjay B. Patil2, P. K. Mallikarjun3*, H. G. Manoj Kumar1 and N. G. Hanamaratti4

“…The results revealed significant variability within and between families, heritability and genetic advance for all traits. Clones such as SNK 190023, SNK 191722, SNK 190362 and SNK 191801 recorded significantly higher cane yield besides showing high resistance (HR) to foliar diseases such as brown rust, red leaf spot, eye spot, brown spot and pokkah boeng in comparison to the checks Co 09004 (early) and Co 86032 (mid-late). …”
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Alterations of Hematologic and Hematopoietic Parameters in Mice Exposed to Pulsed Electromagnetic Field by Kangchu Li, Zenghui Teng, Zhaohui Liu, Yuxing Zhang, Kaiping Long, Qimei Liao, Ansheng Ni, Guirong Ding, Shirong Ma

“…There was no significant difference in mouse bone marrow nucleated cell number between the control group and each exposed group 7 days after PEMF exposure. The CFU-GM clone number of bone marrow cells and serum GM-CSF level were significantly increased in the 100 and 1000 pulses exposed group but not changed in the 10000 pulses exposed group. …”
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New insights into the mechanism of triphenyl phosphate and its metabolite diphenyl phosphate in diabetic kidney disease by Ting Fang, Qiaoyan Liu, Xinxin Huangfu, Hongkai Zhu, Hongwen Sun, Liming Chen

“…Toxicity analysis showed that TPHP could cause dose-dependent toxicity in mouse podocyte clone 5 (MPC5) and mouse mesangial cells (MES13). …”
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Oral Immunotherapy Using Probiotic Ice Cream Containing Recombinant Food-Grade Lactococcus lactis Which Inhibited Allergic Responses in a BALB/c Mouse Model by Alireza Vasiee, Fereshteh Falah, Mojtaba Sankian, Farideh Tabatabaei-Yazdi, Seyed Ali Mortazavi

“…The main gene of this allergen, Ama r 2, was cloned into the food grade plasmid pNZ7025 and then was electrotransformed into the food grade Lactococcus lactis NZ1330. …”
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Spatio‐Temporal Patterns of Hybridization in an Alloploid Salamander (Ambystomatidae: Ambystoma) and Conservation Implications of Introgression in a Unisexual Vertebrate by Alessa V. Laserna, Christopher A. Phillips, Andrew R. Kuhns, Mark A. Davis, Joel B. Corush, Ken N. Paige

“…Gynogenetic reproduction involving LJJ and its host A. texanum should result in a clone of the mother (LJJ); however, with the occurrence of kleptogenesis, it often can result in tetraploid hybrids (LJJT). …”
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Genomic, Evolutionary, and Pathogenic Characterization of a New <i>Polerovirus</i> in Traditional Chinese Medicine <i>Viola philippica</i> by Yuanling Chen, Gaoxiang Chen, Jiaping Yu, Yali Zhou, Shifang Fei, Haorong Chen, Jianxiang Wu, Shuai Fu

“…Two potential recombination events were identified within ORF2, ORF3a, and ORF3, providing insights into the genetic diversity and evolution history of this novel polerovirus. An infectious cDNA clone of VPPV was successfully constructed and shown to infect <i>Nicotiana benthamiana</i>. …”
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A prognostic model of 8-T/B cell receptor-related signatures for hepatocellular carcinoma by Xuan Zuo, Hui Li, Shi Xie, Mengfen Shi, Yujuan Guan, Huiyuan Liu, Rong Yan, Anqi Zheng, Xueying Li, Jiabang Liu, Yifan Gan, Haiyan Shi, Keng Chen, Shijie Jia, Guanmei Chen, Min Liao, Zhanhui Wang, Yanyan Han, Baolin Liao

“…Both the number of unique TCR and BCR clonotypes and the expanded clones were higher in the low-risk group than in the high-risk group. …”
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Uptake of CCR7 by KIR2DS4+ NK Cells Is Induced upon Recognition of Certain HLA-C Alleles by Silvia Pesce, Simona Carlomagno, Alessandro Moretta, Simona Sivori, Emanuela Marcenaro

“…We demonstrate that KIR2DS4 can induce the uptake of CCR7 by KIR2DS4+ NKG2A+ NK cell clones after interacting with CCR7+ target cells expressing HLA-Cw4 and HLA-Cw6 alleles. …”
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In vivo interspecies dissemination of IncM2-type blaNDM-1 carrying plasmid by Mor N. Lurie-Weinberger, Darya Bychenko-Banyas, Meirav Mor, Sivan Laviad-Shitrit, Ella Kaplan, Nadya Rakovitsky, Alona Keren-Paz, Chaim Ben-Zvi, Yehuda Carmeli

“…Such events may cause the emergence of high-risk clones, as well as lead to under-recognized multi-species outbreaks.IMPORTANCEConjugative, carbapenemase-carrying multidrug-resistant plasmids that can move between species of clinically relevant Enterobacterales pose a great risk to patients’ health, especially when they spread inside a medical institution. …”
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