No Correlation Exists between Disease Activity and the Expression of Killer-Cell Immunoglobulin-Like Receptors in Patients with Rheumatoid Arthritis by Toshiaki Kogure, Takeshi Tatsumi, Atsushi Niizawa, Hiroshi Fujinaga, Tomoyuki Ito, Yutaka Shimada, Katsutoshi Terasawa

“…The genes for killer-cell immunoglobulin-like receptors (KIRs) have been cloned and their functions and expression in patients with rheumatoid arthritis (RA) have been partially clarified. …”
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Feasibility of Ex Vivo Ligandomics by Prabuddha Waduge, Remya Ammassam Veettil, Bojun Zhang, Chengchi Huang, Hong Tian, Wei Li

“…However, in vivo ligandomics requires intracardial perfusion to remove unbound phage clones, limiting its use to vascular endothelial cells (ECs). …”
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Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases by Athanasios Mavropoulos, Dimitrios P. Bogdanos, Christos Liaskos, Timoklia Orfanidou, Theodora Simopoulou, Efterpi Zafiriou, Lazaros I. Sakkas, Eirini I. Rigopoulou

“…To overcome these limitations, we have modified methanol-based phosphoflow protocols using several commercially available antibody clones suitable for surface antigens, intracellular cytokines, and p38 MAPK. …”
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Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR by Martina Fazzina, Matteo Bergonzoni, Francesca Massenzio, Barbara Monti, Flavia Frabetti, Raffaella Casadei

“…Recently, the Human Microglial Clone 3 cell line (HMC3) has been characterized and a new human microglia model has emerged. …”
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The genomic copy number determination of improved Cre in transgenic mice using standards derived from megaprimer PCR constructed tandem repeats by Ge Diao, Min Tian, Runbo Li, Jie Huang, Jian Han, Jianxin Guo

“…Two rounds of megaprimer PCR were used to obtain specific tandem repeats of iCre fragments, which were subsequently cloned into pUCm-T vectors. After the fragments of IL-2 (reference gene) were ligated into the same vectors, standards with copy number ratios (CNR) of 0.5, 1, 2, 4, 8, and 16 between iCre and IL-2 were completed. …”
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Cyclic di AMP phosphodiesterase nanovaccine elicits protective immunity against Burkholderia cenocepacia infection in mice by Wesam E. Gawad, Yosra I. Nagy, Tamer M. Samir, Ahmed Mohamed Ibrahim Mansour, Omneya M. Helmy

“…Applying strict subtractive channels, three proteins were shortlisted: WP_006481710.1 (LY), WP_012493605.1 (KT), and WP_006492970.1 (BD). Proteins were cloned, purified as His-tagged proteins, and loaded onto C:P NPs. …”
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The Microtubule-Associated Protein Tau and Its Relevance for Pancreatic Beta Cells by Magdalena Maj, Gregor Hoermann, Sazan Rasul, Wolfgang Base, Ludwig Wagner, Johannes Attems

“…We found upregulation of MAPT protein expression in human insulinomas when compared to human pancreatic islets of Langerhans and an imbalance between MAPT isoforms in insulinomas tissue. We cloned one 3-repeat domain MAPT and transduced this into a beta-cell derived rodent cell line Rin-5F. …”
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Standardization of Micropropagation for Four Hybrids of <i>Coffea arabica</i> Through Direct Somatic Embryogenesis by Marileydi Zuta-Puscan, Jegnes Benjamín Meléndez-Mori, Eyner Huaman-Huaman, Yoiner Kalin Lapiz-Culqui, Reyna Stefani Mego-Pérez, Manuel Oliva-Cruz

“…Direct somatic embryogenesis represents a fundamental tool for obtaining genetically homogeneous clones; however, its commercial scaling faces critical challenges at various stages of the process. …”
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Molecular Characterization and Expression of <i>unc-13d</i> in the Sex Reversal of <i>Monopterus albus</i> by Zitong Lian, Fang Meng, Xueping Xia, Junchao Fang, Haifeng Tian, Qiaomu Hu

“…In this study, we firstly cloned and characterized the sequence and protein structure of <i>unc-13d</i> of <i>M. albus.…”
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The Chloroplastic Small Heat Shock Protein Gene KvHSP26 Is Induced by Various Abiotic Stresses in Kosteletzkya virginica by Xiaohua Liu, Lizi Zhao, Jianzhao Li, Lijun Duan, Kai Zhang, Xuqiang Qiao, Weihuan Li, Chengchao Zheng, Xiaoli Tang, Hongxia Zhang

“…In this work, a putative sHSP gene KvHSP26 was cloned from K. virginica. Bioinformatics analyses revealed that KvHSP26 encoded a chloroplastic protein with the typical features of sHSPs. …”
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Molecular Diversity of Sapovirus Infection in Outpatients Living in Nanjing, China (2011–2013) by Hong-ying Zhang, Meng-kai Qiao, Xuan Wang, Min He, Li-min Shi, Guo-xiang Xie, Hei-ying Jin

“…The PCR products were cloned into pGEM-T Easy vector and bidirectionally sequenced. …”
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Prolactin Levels Correlate with Abnormal B Cell Maturation in MRL and MRL/lpr Mouse Models of Systemic Lupus Erythematosus-Like Disease by Maria Victoria Legorreta-Haquet, Rocio Flores-Fernández, Francisco Blanco-Favela, Ezequiel M Fuentes-Pananá, Luis Chávez-Sánchez, Rafael Hernández-González, Emiliano Tesoro-Cruz, Lourdes Arriaga-Pizano, Adriana Karina Chávez-Rueda

“…These results identify an important role of PRL in the early stages of the B-cell maturation process: PRL may promote the survival of self-reactive clones.…”
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Bioinformatics Analysis, Prokaryotic Expression and Biological Activity of Lysin from Vibrio parahaemolyticus Phage vB_VpP_1 by ZHANG Defu, YANG Wenjing, LIU Ke, LIU Qingqing, BAI Wutong, LI Fan, LÜ Xinran, BAI Xue, TAN Xiqian, LI Xuepeng, LI Jianrong

“…Primers were designed using Primer 5.0 software and cloned into the pET-28a(+) vector for prokaryotic expression. …”
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Clonal Populations of Amniotic Cells by Dilution and Direct Plating: Evidence for Hidden Diversity by Patricia G. Wilson, Lorna Devkota, Tiffany Payne, Laddie Crisp, Allison Winter, Zhan Wang

“…More than 40 clonal populations were recovered from 4 amniocentesis samples and representative clones were characterized by flow cytometry, conventional assays for differentiation potential, immunofluorescence imaging, and transcript analysis. …”
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lncRNA ZFAS1 Promotes HMGCR mRNA Stabilization via Binding U2AF2 to Modulate Pancreatic Carcinoma Lipometabolism by Luoluo Wang, Yi Ruan, Xiang Wu, Xinhua Zhou

“…Measurements of BxPC-3 cell multiplication and invasiveness employed CCK-8, plate clone formation test, and Transwell chamber assay. …”
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Scutellarin combined with lidocaine exerts antineoplastic effect in human glioma associated with repression of epidermal growth factor receptor signaling. by Xiu-Ying He, Yui-Si Yang, Yue-Xiang Zheng, Qing-Jie Xia, Hong-Zhou Yu, Xiao-Ming Zhao, Ting-Hua Wang

“…Cell proliferation was detected by cell counting kit-8 (CCK8), plate clone formation assay, EdU incorporation assay and xCELLigence real-time cell analyzer. …”
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Characterization of Nora Virus Structural Proteins via Western Blot Analysis by Brad L. Ericson, Darby J. Carlson, Kimberly A. Carlson

“…ORF3, ORF4a, ORF4b, and ORF4c were individually cloned and expressed in E. coli; resultant recombinant proteins purified and were used to make monospecific antisera. …”
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The Conservative Physiology of the Immune System. A Non-Metaphoric Approach to Immunological Activity by Nelson M. Vaz, Gustavo C. Ramos, Vitor Pordeus, Claudia R. Carvalho

“…In the late 50s, selective theories were proposed and from then on, immunology has been based in a close association with the neo-Darwinian principles, such as random generation of variants (lymphocyte clones), selection by extrinsic factors (antigens)—and, more generally, on genetic determinism and functionalism. …”
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A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies by Huifang Chen, Zehong Zou, Ailin Tao

“…The splenocytes of immunized mice were fused with myeloma cells (SP2/0), and stable mAb-producing clones were obtained by limiting dilution. mAbs against Ara h 2 were isolated from mouse ascites, and specificity was confirmed by immunoblotting. …”
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Donors of effective genes for scald resistance in barley by G. S. Konovalova, E. E. Radchenko

“…The experiments employed isolates and clones of R. commune isolated from plants collected in Leningrad Province. …”
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