Isolation, identification, and characterisation of the malachite green detoxifying bacterial strain Bacillus pacificus ROC1 and the azoreductase AzrC by Shanza Bibi, Callum W. Breeze, Vusqa Jadoon, Anum Fareed, Alina Syed, Rebecca L. Frkic, Habiba Zaffar, Muhammad Ali, Iftikhar Zeb, Colin J. Jackson, Tatheer Alam Naqvi

“…Genomic sequencing identified three candidate azo-reductases within B. pacificus ROC1 that could be responsible for the MG-degrading activity. These were cloned, expressed and purified from Escherichia coli, with one (AzrC), catalyzing the reduction of MG to leuco-MG in vitro. …”
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PPARγ Regulates Genes Involved in Triacylglycerol Synthesis and Secretion in Mammary Gland Epithelial Cells of Dairy Goats by Hengbo Shi, Jun Luo, Jiangjiang Zhu, Jun Li, Yuting Sun, Xianzi Lin, Liping Zhang, Dawei Yao, Huaiping Shi

“…To explore the function of PPARγ in the goat mammary gland, we cloned the whole cDNA of the PPARγ gene. Homology alignments revealed that the goat PPARγ gene is conserved among goat, bovine, mouse, and human. …”
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Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells by Yazi Huang, Beilei Zhao, Yahan Liu, Nanping Wang

“…Genomic fragment containing 1.7-kilobase of the promoter region was cloned by using PCR. The luciferase reporter assays demonstrated that overexpression of PPARγ and rosiglitazone, a specific ligand for PPARγ, could significantly upregulate the reporter activity. …”
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Identification and Characterization of a Novel Rat MAVS Variant Modulating NFκB Signaling by Ihsan Nalkiran, Hatice Sevim Nalkiran

“…In this study, we identified and characterized a novel rat MAVS variant (MAVS500) with a twenty-one-nucleotide deletion, resulting in a protein seven amino acids shorter than the wild-type (WT) rat MAVS. The MAVS500 was cloned from the rat bladder cancer cell line, NBT-II, using specific primers, and subsequently sequenced. …”
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Molecular Characterization of Antibiotic Resistance and Genetic Diversity of Klebsiella pneumoniae Strains by Bahareh Arabzadeh, Zeynab Ahmadi, Reza Ranjbar

“…Also, the results obtained from genotyping indicated that the K. pneumoniae strains isolated in this study belonged to the diverse clones.…”
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A phenomenological model of non-genomic variability of luminescent bacterial cells by S. I. Bartsev

“…All experiments were carried out with bacterial clones (genetically homogeneous populations). A typical dynamics of luminous bacterial cells distributions with respect to intensity of light emission at various stages of batch culture growth in a liquid medium was obtained. …”
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Cellular automata modelling of leukaemic stem cell dynamics in acute myeloid leukaemia: insights into predictive outcomes and targeted therapies by Yutaka Saikawa, Toshihiko Komatsuzaki, Nobuaki Nishiyama, Toshihisa Hatta

“…Our study revealed: (i) a correlation between LSC persistence post-induction chemotherapy and risk of AML relapse; (ii) MRD negativity based on LSC count may not reliably predict outcomes, supporting clinical evidence that patients with MRD-negative status can still be at risk of relapse; (iii) prolonged persistence of LSCs post-chemotherapy without disruption of normal haematopoiesis, aligning with clinical observations of dormant AML clones; (iv) early LSC dynamics post-induction chemotherapy, characterized by stochastic behaviours and movement velocities, are insufficient predictors of long-term prognosis; and (v) a distinct spatiotemporal organization of LSCs in later phases post-induction chemotherapy is correlated with long-term outcomes. …”
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Expressional Subpopulation of Cancers Determined by G64, a Co-regulated Module by Jae-Woong Min, Sun Shim Choi

“…Studies of cancer heterogeneity have received considerable attention recently, because the presence or absence of resistant sub-clones may determine whether or not certain therapeutic treatments are effective. …”
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Postharvest Analysis of Lowland Transgenic Tomato Fruits Harboring hpRNAi-ACO1 Construct by Bita Behboodian, Zainon Mohd Ali, Ismanizan Ismail, Zamri Zainal

“…Initially, cDNA corresponding to ACO1 of lowland tomato cultivar (MT1), which has high identity with ACO1 of Solanum lycopersicum in GenBank, was cloned through RT-PCR. Using a partial coding region of ACO1, one hpRNAi transformation vector was constructed and expressed ectopically under the 35S promoter. …”
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Polymyalgia Rheumatica Revealing a Lymphoma: A Two-Case Report by Frank Verhoeven, Xavier Guillot, Mickaël Chouk, Clément Prati, Daniel Wendling

“…Blood exams highlighted the presence of a monoclonal B cell clone. Bone marrow concluded to a B cell lymphoma of the marginal zone. …”
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Determination of mutagenicity of chemical compounds, physical factors and environmental pollutants by the Drosophila melanogaster wing somatic mutation and recombination test by L. P. Zakharenko, I. K. Zakharov

“…Recombination or mutational events in the cell leads to the formation of mutant spots/clones visible by microscopic analysis of the wing surface. …”
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System Efficiency of a Tap Transformer Based Grid Connection Topology Applied on a Direct Driven Generator for Wind Power by Senad Apelfröjd, Sandra Eriksson

“…A full experimental set up of the system has been used in the laboratory study, where a clone of the on-site generator was driven by an induction motor and the system was connected to a resistive load to better evaluate the performance. …”
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Caracterización, procesabilidad y viabilidad técnica de fondos de taza de caucho natural by Natalia Cardona Vásquez, Diego Hernán Giraldo Vásquez, Sandra Milena Velásquez Restrepo

“… En este artículo, se compararon propiedades fsicoquímicas, espectroscópicas, de resistencia a la termooxidación y de procesabilidad de fondos de taza de caucho natural de los clones IAN 873 y FX 3864 respecto del caucho natural técnicamente especifcado grado 10 proveniente de Guatemala, uno de los más importados en Colombia y en América Latina. …”
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DISEASES OF NATURAL RUBBER (HEAVEA BRASILENSIS) INDUCED BY THE CLIMATIC ELEMENTS OF RAINFALL AND TEMPERATURE IN IKPOBA-OKHA LOCAL GOVERNMENT AREA OF EDO STATE, NIGERIA by ABRAHAM EHIZOJIE ILENRE

“…The following recommendations are made: effective disease management strategies, monitoring of climatic conditions, incorporating resistant natural rubber clones and improving cultural practices, sustainable practices to manage diseases, governments’ intervention to encouragenatural rubber farmers annually. …”
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Improvement of the technique applied to preserve species and varieties of <i>Fragaria</i> L. in the field genebank at Krymsk Experiment Breeding Station of VIR by V. N. Podorozhniy, N. A. Piyanina

“…The accessions are sources and donors of traits important for breeding and represent the most valuable material for targeted crossings in programs developing and releasing strawberry cultivars, primarily garden ones.The effectiveness of their utilization can be seen in the adaptable high-yielding cultivars developed at Krymsk Station in recent years: ‘Zenkora’ (Zenga-Zengana × Cardinal) was patented, and ‘Pelageya’ [Elizaveta II (clone Coroleva Elizaveta) × Irma] included in the State Register of the Russian Federation. …”
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Use of Posttranscription Gene Silencing in Squash to Induce Resistance against the Egyptian Isolate of the Squash Leaf Curl Virus by Omnia Taha, Inas Farouk, Abdelhadi Abdallah, Naglaa A. Abdallah

“…In this study full-length genomic clone of SqLCV Egyptian isolated and posttranscriptional gene silencing (PTGS) has been induced to develop virus resistance. …”
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Physically non-cloneable arbiter-type function with non-linear path pairs by A. A. Ivaniuk, A. Y. Shamyna

“…The set of all challenge-response pairs is a random, unpredictable and irreproducible in the cases of implementation of cloned PUF circuits both on single and/or on another chips, also using different technologies. …”
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CAR T cells for treating autoimmune diseases by Dimitrios Mougiakakos, Ulrike Koehl, Stephan Fricke, Ulrich Blache, Sandy Tretbar

“…Furthermore, in preclinical models, researchers have also developed chimeric autoantibody receptor T cells that can eliminate individual B cell clones producing specific autoantibodies, and regulatory CAR T cells that do not eliminate autoreactive immune cells but dampen their wrong activation. …”
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TCR Gene Transfer: MAGE-C2/HLA-A2 and MAGE-A3/HLA-DP4 Epitopes as Melanoma-Specific Immune Targets by Trudy Straetemans, Mandy van Brakel, Sabine van Steenbergen, Marieke Broertjes, Joost Drexhage, Joost Hegmans, Bart N. Lambrecht, Cor Lamers, Pierre van Der Bruggen, Pierre G. Coulie, Reno Debets

“…We molecularly characterized TCRαβ genes of an MC2/A2-specific CD8 and MA3/DP4-specific CD4 T-cell clone derived from melanoma patients who responded clinically to MAGE vaccination. …”
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Potential of a novel and thermostable recombinant phage endolysin of Escherichia phage KW1E_UTAR against Shigella spp. by Selvam Dharshini, Mohd Ismail Nor Ismaliza, Ng Michelle Y. T.

“…In this study, the Escherichia phage KW1E_UTAR gene encoding for endolysin was successfully cloned and expressed in Escherichia coli pET-28a. …”
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