Stem Cell Therapy for Diseases of Livestock Animals: An In-Depth Review by Raghavendra B. Narasimha, Singireddy Shreya, Vijay Anand Jayabal, Vikas Yadav, Prasana Kumar Rath, Bidyut Prava Mishra, Sudhakar Kancharla, Prachetha Kolli, Gowtham Mandadapu, Sudarshan Kumar, Ashok Kumar Mohanty, Manoj Kumar Jena

“…Current development in stem cell research has widened their application in cell therapy, drug discovery, reproductive cloning in animals, and cell models for various diseases. …”
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Ecdysone receptor strongly influences larval–pupal–adult transition and melanization in Tuta absoluta by Xiaodi Wang, Jiajia Wu, Jianyang Guo, Nianwan Yang, Fanghao Wan, Zhichuang Lü, Wanxue Liu

“…Methods In this study, RNA extraction, cDNA synthesis, gene cloning, bioinformatics analysis, and quantitative real-time polymerase chain reaction were used to obtain the full length, conserved domain, and relative expression levels of the EcR. …”
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QTL mapping and candidate gene analysis of element accumulation in rice grains via genome-wide association study and population genetic analysis by Min Li, Mengxue Gao, Jinmei Huang, Shaojuan Jin, Yamei Lv, Lin Wang, Tao Huang, Fengcai Wu, Zhijian Xu, Yinghua Pan, Zhixia Liu, Chaopu Zhang, Yuntao Liang, Lijun Meng, Jianlong Xu

“…More importantly, population genetic analysis revealed 26 and 15 intraspecies divergent regions affecting element concentrations in the Xian and Geng subspecies, respectively, including 25 QTLs identified in this study and 13 previously reported and cloned genes. Conclusions Our findings will facilitate further gene cloning and dissection of the genetic mechanisms of element accumulation in rice grains to improve grain quality.…”
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Molecular Characterization of a Multidrug-Resistant Klebsiella pneumoniae Strain R46 Isolated from a Rabbit by Fei Wu, Yuanyuan Ying, Min Yin, Yi Jiang, Chongyang Wu, Changrui Qian, Qianqian Chen, Kai Shen, Cong Cheng, Licheng Zhu, Kewei Li, Teng Xu, Qiyu Bao, Junwan Lu

“…To investigate the mechanisms of multiple resistance and the horizontal transfer of resistance genes in animal pathogens, we characterized the molecular structures of the resistance gene-related sequences in a multidrug-resistant Klebsiella pneumoniae strain R46 isolated from a rabbit. Molecular cloning was performed to clone the resistance genes, and minimum inhibitory concentrations (MICs) were measured to determine the resistance characteristics of the cloned genes and related strains. …”
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circASAP1 induces renal clear cell carcinoma ferroptosis by binding to HNRNPC and thereby regulating GPX4 by Yunfei Wang, Taowei Yang, Qihao Li, Zhousan Zheng, Lican Liao, Junjie Cen, Wei Chen, Junhang Luo, Yi Xu, Mi Zhou, Jiaxing Zhang

“…High circASAP1 expression was associated with ccRCC cell proliferative viability, invasion, and metastasis in CCK-8, transwell, plate cloning, and EdU experiments. Interaction of circASAP1 with HNRNPC and their downstream target genes was confirmed by RNA pull-down, RNA immunoprecipitation, FISH, silver staining, and mass spectrometry. …”
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Efficient Acquisition of Fully Human Antibody Genes against Self-Proteins by Sorting Single B Cells Stimulated with Vaccines Based on Nitrated T Helper Cell Epitopes by Liangliang Jiang, Tao Jiang, Jianhua Luo, Yanliang Kang, Yue Tong, Xiaoda Song, Xiangdong Gao, Wenbing Yao, Hong Tian

“…We randomly selected eight genes for cloning, six of which expressed antibodies that can bind to HER2. …”
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Identification of QTL for Plant Height Traits in Single-segment Substitution Lines of Wild Rice by Xingxin DAI, Yanjie LIU, Chengxia LI, Rui TANG, Jiamei MU, Xuelin FU

“…It lays a foundation for further cloning of the elite genes for plant height in wild rice, and provides valuable genetic resources for breeding rice varieties with ideal plant height.…”
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Wisdom of the Established Pattern by Joel Janhonen

“…The Wisdom of Repugnance: Why We Should Ban the Cloning of Humans. Val. U. L. Rev. 32(2), 679–705. https://scholar.valpo.edu/vulr/vol32/iss2/12 [3] Mameli, M. (2013). …”
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Designing a multi-epitope vaccine candidate against human rhinovirus C utilizing immunoinformatics approach by Tajul Islam Mamun, Tajul Islam Mamun, Md. Ahad Ali, Md. Nazmul Hosen, Jillur Rahman, Md. Anwarul Islam, Md. Golam Akib, Kamruz Zaman, Md. Masudur Rahman, Md. Masudur Rahman, Ferdaus Mohd Altaf Hossain, Ferdaus Mohd Altaf Hossain, Samir Ibenmoussa, Mohammed Bourhia, Turki M. Dawoud

“…Furthermore, in silico cloning and sequence optimization ensured efficient expression in E. coli, with a Codon Adaptation Index of 0.99 and GC content of 54.58%. …”
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Dissection of the genetic basis and molecular mechanism of ovule number per ovary in oilseed rape (Brassica napus L.) by Muslim Qadir, Muslim Qadir, Muslim Qadir, Xinyi Lin, Farhan Nabi, Kishore Kumar Ashok, Xue-Rong Zhou, Qingbin Sun, Peiman Shi, Xinfa Wang, Jiaqin Shi, Hanzhong Wang

“…These findings propose further insights into the genetic basis and molecular mechanisms of ONPO, which will facilitate future gene cloning and genetic improvement for enhancing seed yield in oilseed rape.…”
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Fine-mapping of PmHHM, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew by Bisheng Fu, Bisheng Fu, Bisheng Fu, Zhixin Lin, Lijuan Yan, Qiaofeng Zhang, Caiyun Liu, Caiyun Liu, Jin Cai, Jin Cai, Wei Guo, Wei Guo, Ying Liu, Wenling Zhai, Shuangjun Gong, Feng Xu, Jizhong Wu, Jizhong Wu, Jizhong Wu

“…Comparative genomic analysis revealed presence/absence variations (PAVs) of the PmHHM locus among common wheat varieties.DiscussionThese closely linked molecular markers will not only benefit the cloning of the gene underlying PmHHM but also facilitate the efficient utilization of the gene in breeding programs.…”
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Identification and expression analysis of c-type and g-type lysozymes genes after Aeromonas hydrophila infection in African catfish by Hasan Nasrullah, Yanti Inneke Nababan, Dwi Hany Yanti, Dian Hardiantho, Sri Nuryati, Muhammad Zairin Junior, Julie Ekasari, Alimuddin Alimuddin

“…In the present study, we report a partial cloning and mRNA expression analysis of c-type and g-type lysozymes in African catfish Clarias gariepinus. …”
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OsLAP3/OsSTRL2, encoding a rice strictosidine synthase, is required for anther cuticle formation and pollen exine patterning in rice by Dai-bo Chen, Ran Zhou, Ran Zhou, Hui-min Wang, Pei-pei Zhang, Zheng-fu Yang, Zheng-fu Yang, Dan-dan Xuan, Ying-xin Zhang, Xiao-deng Zhan, Li-yong Cao, Li-yong Cao, Shi-hua Cheng, Lian-ping Sun, Lian-ping Sun

“…In this study, a male-sterile rice mutant, lap3, was identified, characterized by normal vegetative growth but complete male sterility due to delayed programmed cell death (PCD) in tapetal cells and defects in anther cuticle and pollen exine formation. Map-based cloning revealed that OsLAP3 is a new allele of the strictosidine synthase-like gene, OsSTRL2. …”
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Analysis of Nucleotide Alterations in the E6 Genomic Region of Human Papillomavirus Types 6 and 11 in Condyloma Acuminatum Samples from Brazil by Marina Carrara Dias, Bruna Stuqui, Paola Jocelan Scarin Provazzi, Cíntia Bittar, Natália Maria Candido, Renata Prandini Adum de Matos, Rodolfo Miglioli Badial, Caroline Measso do Bonfim, Patricia Pereira dos Santos Melli, Silvana Maria Quintana, José Antônio Cordeiro, Paula Rahal, Marilia de Freitas Calmon

“…The products were ligated to a cloning vector and five colonies of each sample were sequenced to observe the nucleotide alterations. …”
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USP14 inhibition by degrasyn induces YAP1 degradation and suppresses the progression of radioresistant esophageal cancer by Fang Yuan, Juan Xu, Lingmei Xuan, Chan Deng, Wei Wang, Rong Yang

“…Additionally, we used CCK8, cloning, and migration tests to examine the proliferation and migration of ESCA cells. …”
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ZC3H15 suppression ameliorates bone cancer pain through inhibiting neuronal oxidative stress and microglial inflammation by Li-Quan Huang, Ting-Xuan Yan, Bao-Sheng Wang, Hao Li, Nai-Bao Zhou

“…Additionally, we used CCK8, cloning, and migration tests to examine the proliferation and migration of cancer cells. …”
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Investigating novel Streptomyces bacteriophage endolysins as potential antimicrobial agents by Jindanuch Maneekul, Amanda Chiaha, Rachel Hughes, Faith Labry, Joshua Saito, Matthew Almendares, Brenda N. Banda, Leslie Lopez, Nyeomi McGaskey, Melizza Miranda, Jenil Rana, Brandon R. Zadeh, Lee E. Hughes

“…From 250 putative endolysins, bioinformatic methods were used to select three putative endolysins for cloning and study: LL35lys, Nb26lys, and Tb42lys. …”
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A Series of Novel Alleles of <i>Ehd2</i> Modulating Heading and Salt Tolerance in Rice by Peng Xu, Shulei Hao, Xiaoxia Wen, Guifang Ma, Qinqin Yang, Ling Liu, Galal Bakr Anis, Yingxin Zhang, Lianping Sun, Xihong Shen, Qunen Liu, Daibo Chen, Yongbo Hong, Yuyu Chen, Xiaodeng Zhan, Shihua Cheng, Liyong Cao, Weixun Wu

“…Using MutMap and map-based cloning, the causative gene was identified as a novel allele of <i>Ehd2</i>/<i>OsID1</i>/<i>RID1</i>/<i>Ghd10</i>. …”
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Loop33 × 123 CAR-T targeting CD33 and CD123 against immune escape in acute myeloid leukemia by Haotian Ma, Zhifeng Yan, Runxia Gu, Yingxi Xu, Shaowei Qiu, Haiyan Xing, Kejing Tang, Zheng Tian, Qing Rao, Min Wang, Jianxiang Wang

“…Methods Based on our previously constructed CD33 and CD123 monovalent CAR-T, Loop33 × 123 and Loop123 × 33 CAR-T were constructed with molecular cloning techniques. All CAR-T cells were generated by lentivirus transduction of T cells from healthy donors. …”
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Production de Pennisetum sp. et son utilisation pour la culture de Pleurotus ostreatus au Burundi by Prosper Kiyuku, Samuel Bigawa

“…The results obtained showed that clones S3 and S4 of Pennisetum sp. had a more important average stem and leaf productivity than S1 and S2 clones. …”
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