Unlocking the potential of cacao yield with full sun cultivation by Carolina S. Benjamin, Luiz A. S. Dias, Samuel C. V. Martins, Carlos E. Aucique-Perez, Lucas B. C. Rosmaninho

“…These A high values suggest the clones are well-acclimatized to full sun cultivation. …”
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Plant diversity and transcriptional variability assessed by retrotransposon-based molecular markers by R. N. Kalendar, K. S. Aizharkyn, O. N. Khapilina, A. A. Amenov, D. S. Tagimanova

“…Molecular markers have become crucial part of genetics due to their use in various branches of it, such as positional cloning, which includes identification of genes responsible for desired traits and management of backcrossing programs, as well as in modern plant breeding, and human forensics. …”
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Astrobiological Field Campaign to a Volcanosedimentary Mars Analogue Methane Producing Subsurface Protected Ecosystem: Imuruk Lake (Alaska) by F. Gómez, O. Prieto-Ballesteros, D. Fernández-Remolar, J. A. Rodríguez-Manfredi, M. Fernández-Sampedro, M. Postigo Cacho, J. Torres Redondo, N. Rodríguez, J. Gómez-Elvira, R. Amils

“…Different conventional (enrichment and isolation) and molecular ecology techniques (cloning, fluorescence “in situ” probe hybridization, FISH) have been used for isolation and bacterial identification.…”
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Immortalized Mesenchymal Stem Cells: A Safe Cell Source for Cellular or Cell Membrane-Based Treatment of Glioma by Yuxuan Zhang, Jie Liu, Yunzhao Mo, Zetao Chen, Taoliang Chen, Yan Li, Yaofeng Zheng, Shaokang Deng, Xiangdong Xu, Huajian Chen, Haoqi He, Jiansheng Chen, Tao Jin, Xinlin Sun, Yiquan Ke, Jihui Wang

“…The tumorigenicity of engineered MSCs was evaluated using soft agar cloning assays. Next, the engineered cells were injected into the brain of female BALB/c nude mice. …”
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Prevalence of the Helicobacter pylori babA2 Gene in Children Mainly Depends on the PCR Primer Set Used by Anja Šterbenc, Maja M. Lunar, Matjaž Homan, Boštjan Luzar, Nina Zidar, Mario Poljak

“…The 271 bp PCR primer set missed 32 babA2 cases that were 832 bp and/or 139 bp PCR positive, but tested solely positive in 109 cases. Interestingly, cloning of a subset of 271 bp PCR positive samples revealed amplification of the babA/B gene chimera. …”
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Receptor-like Kinase GOM1 Regulates Glume-Opening in Rice by Xinhui Zhao, Mengyi Wei, Qianying Tang, Lei Tang, Jun Fu, Kai Wang, Yanbiao Zhou, Yuanzhu Yang

“…<i>gom1</i> exhibits dysfunctional lodicules that lead to open glumes following anthesis. Map-based cloning and subsequent complementation tests confirmed that GOM1 encodes a receptor-like kinase (RLK). …”
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Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis by Yang Sun, Sanmao Su, Mengjun Li, Ang Deng

“…Cell counting kit-8 (CCK8), plate cloning assay, and flow cytometry were conducted to evaluate cell proliferation and apoptosis. …”
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Promising markers of CIMP+ colon tumors identified on the basis of TCGA data analysis by G. S. Krasnov, A. D. Beniaminov, R. A. Tychko, G. A. Puzanov, R. O. Novakovskiy, A. V. Kudryavtseva, A. A. Dmitriev

“…Then, the bisulfite conversion of DNA followed by cloning and sequencing was applied to examine the methylation status of CpG sites that were selected as the result of bioinformatics analysis. …”
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Production of recombinant norovirus VP1 protein and its antigenic and immunogenic properties by Vladislav A. Lapin, Dmitry V. Novikov, Ekaterina V. Mokhonova, Dmitry A. Melentyev, Maria I. Tsyganova, Dmitry E. Zaitsev, Viktor V. Novikov

“…Computer analysis of nucleotide and amino acid sequences, molecular cloning, polymerase chain reaction, electrophoresis of nucleic acids in agarose gel and proteins in polacrylamide gel, affinity chromatography, enzyme immunoassay. …”
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Chromosome-scale assembly and annotation of the wild wheat relative Aegilops comosa by Hongna Li, Shams ur Rehman, Rui Song, Liang Qiao, Xiaohua Hao, Jing Zhang, Kairong Li, Lifeng Hou, Wanyi Hu, Le Wang, Shisheng Chen

“…This newly constructed reference genome of Ae. comosa will serve as an important genomic resource for comparative genomic studies and the cloning of agriculturally important genes.…”
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An Effective and Safe Maize Seed Chipping Protocol Using Clipping Pliers With Applications in Small-Scale Genotyping and Marker-Assisted Breeding by Brian Zebosi, John Ssengo, Lander Geadelmann, Erica Unger-Wallace, Erik Vollbrecht

“…In applications such as marker-assisted breeding and positional cloning, tissue sampling and plant tracking are vital steps in the genotyping pipeline. …”
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Construction of novel pJRD215-derived plasmids using chloramphenicol acetyltransferase (cat) gene as a selection marker for Acidithiobacillus caldus. by Rui Wang, Chunmao Lin, Jianqiang Lin, Xin Pang, Xiangmei Liu, Chengjia Zhang, Jianqun Lin, Linxu Chen

“…A new vector pSDU1 carrying the replication and mobilization regions derived from pJRD215, the P2-cat gene cassette and a multiple cloning site from pUC19 was successfully constructed. …”
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Biochemical properties and substrate specificity of GOB-38 in Elizabethkingia anophelis by Ren Liu, Yang Liu, Jiehui Qiu, Qun Ren, Chunping Wei, Dejin Pan, Jianglong Shi, Peng Liu, DanDan Wei, Tianxin Xiang, Na Cheng

“…Our findings indicate that the enzyme GOB-38 displays a wide range of substrates, including broad-spectrum penicillins, 1–4 generation cephalosporins, and carbapenems, potentially contributing to in vitro drug resistance in E. coli through a cloning mechanism. It is important to highlight that GOB-38 exhibits a distinct active site composition compared to GOB-1/18, featuring hydrophilic amino acids Thr51 and Glu141 at both ends of its active center instead of hydrophobic alanine, potentially indicating a preference for imipenem. …”
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Efficient regeneration protocol for producing true-to-type oil palm (Elaeis guineensis (jacq.) through somatic embryogenesis from immature male inflorescence by B. Kalyana Babu, R.K. Mathur, K. Suresh, G. Ravichandran, B. Susanthi, G.B. Patil, Narayana Ruthweek, M. Mahesh

“…This protocol will be instrumental in producing homogeneous oil palm planting material through micropropagation and cloning of mother palms, facilitating the establishment of semi-clonal seed gardens.…”
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Advances in Molecular Genetics of Reproductive Isolation in Rice by Shunmei LI, Zihan ZHENG, Ruiying LIU, Hongze GUO, Lin ZHENG, Yongyao XIE

“…The scientists have made great progress in cloning the causal genes and expounding the molecular genetic basis of RI in rice, which not only deepen our theoretical understanding of the rice evolutionary biology, but also greatly promote the development of distant hybrid rice breeding. …”
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Chicken C4BPM facilitates replication of H5N1 and H9N2 Avian Influenza Viruses by inhibiting the production of type I interferons by Huizi Li, Shishi Wang, Ruihong Guo, Xijiao Chen, Yuanyuan Huang, Weixin Jia, Zhangyong Ning

“…To verify the role of C4BPM in replication of AIVs, C4BPM knockout (C4BPM-KO) and stable overexpressing (C4BPM-overexpressing) DF-1 cells were generated using lentivirus-mediated CRISPR/Cas9 gene editing technology and molecular cloning strategies. Replication of H5N1 and H9N2 AIVs were promoted in C4BPM-overexpressing DF-1 cells with inhibited expression of type I IFNs (IFN-α, IFN-β), protein kinase R (PKR), cholesterol 25-hydroxylase (CH25H), interferon regulatory factor (IRF) 3, and mitochondrial antiviral signaling protein (MAVS) compared to that of the DF-1 cells. …”
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Calcitonin Gene-Related Peptide (Cgrp), Adrenomedullin (Am), Amylin, And Calcitonin (Ct) Receptors And Overlapping Biological Actions by J.A. Fischer, W. Born, R. Muff

“…The structure of a CT receptor was elucidated by the group of Goldring in 1991 through molecular cloning, and of a 60% homologous human CT receptor-like receptor (CRLR) shortly thereafter here. …”
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Mapping and Validation of Quantitative Trait Loci on Yield-Related Traits Using Bi-Parental Recombinant Inbred Lines and Reciprocal Single-Segment Substitution Lines in Rice (<i>Or... by Ghulam Ali Manzoor, Changbin Yin, Luyan Zhang, Jiankang Wang

“…Reciprocal SSSLs not only are the ideal genetic materials for QTL validation, but also provide the opportunity for fine mapping and gene cloning of the validated QTLs.…”
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Zinc finger proteins facilitate adaptation of a global insect pest to climate change by Tianpu Li, Jiao Guo, Guilei Hu, Fang Cao, Haiyin Su, Mengdi Shen, Huimin Wang, Minsheng You, Yuanyuan Liu, Geoff M. Gurr, Shijun You

“…We utilized a comprehensive approach involving cloning and bioinformatics analysis of three ZFPs, PxZNF568, PxZNF93, and PxZNF266, measurement of their expression levels in hot-evolved and control strains, and assessment of catalase enzymatic activity and total antioxidant capacity. …”
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Development of a stable eukaryotic strain producing fully human monoclonal antibody on the basis of the human antibody against ectromelia virus by A. L. Matveev, Ya. A. Khlusevich, I. K. Baykov, I. V.  Babkin, E. P. Goncharova, V. V. Morozova, N. V. Tikunova

“…Using this selection system, a stable clone producing target antibody fh8E was selected with the level of production of about 100 μg/ml. …”
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