Sculpting multi-epitope vaccine against Monkeypox viral strains using immunoinformatics by Zaira Rehman, Ammad Fahim, Maryam Irtash

“…Based on docking scores and physicochemical profile the vaccine construct V1 with beta defensin adjuvant was selected for further analysis. In silico cloning of V1 into pBAD-DEST49 vector showed maximum expression in bacterial system. …”
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Investigation and Identification of let-7a Related Functional Proteins in Gastric Carcinoma by Proteomics by Yimin Zhu, Xingyuan Xiao, Lairong Dong, Zhiming Liu

“…The cell line of SGC-7901 stablely overexpressing let-7a was successfully established by gene cloning. Two-dimensional gel electrophoresis (2-DEy was used to separate the total proteins of SGC-7901/let-7a, SGC-7901/EV and SGC-7901, and PDQuest software was applied to analyze 2-DE images. …”
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Expression of the transcription factor encoding gene <i>StTCP23</i> in potato plants infected with the tuber spindle viroid by N. V. Mironenko, A. S. Orina, N. M. Lashina, О. S. Afanasenko

“…Diversity of RNA molecules in the PSTVd population was revealed using RT, cloning, and sequencing of the viroid. The expression of the StTCP23 gene in infected potato plants was assessed by quantitative PCR with the ef1α gene as a reference.Results. …”
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Utilizing the subtractive proteomics approach to design ensemble vaccine against Candida lusitaniae for immune response stimulation; a bioinformatics study. by Habiba Naz, Rimsha Timotheous, Muhammad Farhan Sarwar, Tariq Nadeem, Mudassar Fareed Awan, Sajed Ali, Sophia Awais, Irfan Ahmed

“…Afterwards, the codon optimization was executed, followed by in-silico cloning of the final vaccine construct. The pet28A plasmid was incorporated for this purpose while, the SnapGene tool was utilized for this particular analysis. …”
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Assessment of heat-killed E. coli expressing Chikungunya virus E2 protein as a candidate vaccine for dual protection against Chikungunya virus and E. coli by Surajit Patra, Surajit Patra, Virendra Gajbhiye, Virendra Gajbhiye, Yogesh A. Karpe, Yogesh A. Karpe

“…Escherichia coli (E.coli), a vital part of human gut microbiota, is utilized in recombinant DNA technology for cloning. However, its high adaptability can lead to severe infections in humans. …”
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Peptide-Based Vaccinology: Experimental and Computational Approaches to Target Hypervariable Viruses through the Fine Characterization of Protective Epitopes Recognized by Monoclon... by Matteo Castelli, Francesca Cappelletti, Roberta Antonia Diotti, Giuseppe Sautto, Elena Criscuolo, Matteo Dal Peraro, Nicola Clementi

“…Studying postinfection and postimmunization sera, as well as cloning and characterization of monoclonal antibodies (mAbs), still represents the best approach to identify protective epitopes. …”
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Potato resistance to quarantine diseases by A. V. Khiutti, O. Yu. Antonova, N. V. Mironenko, T. A. Gavrilenko, O. S. Afanasenko

“…Modern data on the genetics of potato resistance to S. endobioticum, G. rostochiensis and G. pallida, including mapping and cloning of R-genes, are presented. Available literature data on molecular markers of R-genes for marker assisted selection and the evaluation of their effectiveness are presented. …”
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The Invasion and Metastasis of Colon Adenocarcinoma (COAD) Induced by SALL4 by Wenjuan Zhang, Yan Hu, Wenbing Zhang, Ke Yi, Xiaohui Xu, Zhihua Chen

“…The influence of SALL4 on COAD cells invasion and proliferation was detected using plate cloning assay and Transwell. The expressions of EMT-related proteins E-cadherin, N-cadherin, vimentin, and Twist were detected by Western blot. …”
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Genome Structure of Bacillus cereus tsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis by Hui Li, Suping Zhou, Terrance Johnson, Koen Vercruysse, Ouyang Lizhi, Parthasarathy Ranganathan, Nsoki Phambu, Alexander J. Ropelewski, Theodore W. Thannhauser

“…The five PHB genes in tsu1 (phaA, phaB, phaR, phaC, and phaP) were cloned and expressed with TOPO cloning, and the recombinant proteins were validated using peptide mapping of in-gel trypsin digestion followed by mass spectrometry analysis. …”
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Molecular complexity of quantitative immunity in plants: from QTL mapping to functional and systems biology by Chauveau, Carine, Roby, Dominique

“…First, from genetic analyses by QTL (Quantitative Trait Locus) mapping and GWAs (Genome Wide Association studies), the identification, cloning and functional analysis of this gene have been used as a starting point for the exploration of the multiple and coordinated pathways acting together to mount the QDR response dependent on RKS1. …”
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Identification of Pseudoperonospora cubensis RxLR Effector Genes via Genome Sequencing by Rahel Dinsa Guta, Marc Semunyana, Saima Arif, Inyong Jeong, Sun Ha Kim, Jiyoung Min, Sang-Keun Oh

“…A total of 45 RxLR effector genes were identified from the genome of P. cubensis. Cloning and functional characterization of these effectors were performed through transient expression assays in Nicotiana benthamiana leaves. …”
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Identifcation and fine mapping of qHSW1, a major QTL for hundred-seed weight in mungbean by Xuesong Han, Xuesong Han, Long Zhao, Juan Yu, Xingmin Wang, Shilong Zhang, Li Li, Li Li, Changyan Liu, Changyan Liu

“…Fine mapping and map-based cloning procedures, along with progeny testing of recombinants, aided in narrowing the candidate interval for qHSW1 to 506 kb. …”
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Construction of Shoot Apical Meristem cDNA Yeast Library of <i>Brassica napus</i> L. and Screening of Proteins That Interact with the Inflorescence Regulatory Factors BnTFL1s by Lingxiong Zan, Haidong Liu, Xutao Zhao, Dezhi Du, Kaixiang Li

“…<i>BnTFL1s</i> (<i>BnaA10.TFL1</i> and <i>BnaC09.TFL1</i>), which encode the transcription factor <i>TERMINAL FLOWER 1 (TFL1),</i> have previously been identified as candidate genes controlling this trait through map-based cloning. However, the mechanism underlying the effects of the BnTFL1 proteins remains unclear. …”
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qTrustNet Virtual Private Network (VPN): Enhancing Security in the Quantum Era by Hyoungsub Shim, Bongho Kang, Haejung Im, Duhyun Jeon, Seok-Min Kim

“…PUFs offer a hardware-based security mechanism that is resistant to cloning and tampering, ensuring that the encryption keys remain secure against both classical and quantum attacks. …”
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Avirulence depletion assay: Combining R gene-mediated selection with bulk sequencing for rapid avirulence gene identification in wheat powdery mildew. by Lukas Kunz, Jigisha Jigisha, Fabrizio Menardo, Alexandros G Sotiropoulos, Helen Zbinden, Shenghao Zou, Dingzhong Tang, Ralph Hückelhoven, Beat Keller, Marion C Müller

“…Wheat resistance breeding frequently relies on the use of resistance (R) genes that encode diverse immune receptors which detect specific avirulence (AVR) effectors and subsequently induce an immune response. While R gene cloning has accelerated recently, AVR identification in many pathogens including Bgt lags behind, preventing pathogen-informed deployment of resistance sources. …”
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Recombinant short TNF-BD protein from smallpox virus is pharmacologically active in an experimental septic shock model by I. P. Gileva, S. N. Yakubitskiy, I. V. Kolosova, S. N. Shchelkunov

“…Recombinant VARV-CrmB protein has been produced in insect cells using molecular cloning methods and its TNF neutralizing activity has been shown in vitro and in vivo. …”
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Diploid Ancestor Tracing of Allopolyploid Cultivars in <i>Camellia reticulata</i> Based on ITS and <i>RPB</i>2 Sequences by Zhifeng Fan, Wei Zheng, Chengmin Yan, Xiaodan Xu

“…This study aims to trace these diploid ancestors by cloning and sequencing ITS from 25 taxa and <i>RPB</i>2 from 21 taxa across various ploidy levels of <i>C. reticulata</i> and its traditional cultivars and related species, combined with their fruit morphology data. …”
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Revealing the diversity of Fusarium micromycetes in agroecosystems of the North Caucasus plains for replenishing the State Collection of Phytopathogenic Microorganisms of the All-R... by N. S. Zhemchuzhina, M. I. Kiseleva, T. M. Kolomiets, I. B. Ablova, A. P. Glinushkin, S. A. Elizarova

“…The effect of the preceding crop on the frequency of Fusarium species isolated from winter wheat was observed. After series cloning of collected isolates, 21 strains of different fungal species characterized by stable morphology traits and known pathogenic and phytotoxic properties were selected for collection replenishment. …”
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Albino lethal 13, a chloroplast‐imported protein required for chloroplast development in rice by Xiaoqiong Guo, Chunli Wang, Qian Zhu, Wenhua Dongchen, Xiaoling Zhang, Wei Li, Hui Zhang, Cui Zhang, Zar Ni Naing Nant Nyein, Mengting Li, Lijuan Chen, Dongsun Lee

“….), named albino lethal 13 (osal13), which displayed a distinct albino phenotype in leaves, ultimately resulting in seedling lethality. Molecular cloning revealed that OsAL13 encodes a novel rice protein with no homologous gene or known conserved domain. …”
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Sarco(endo)plasmic Reticulum Ca2+-ATPase-2 Gene: Structure and Transcriptional Regulation of the Human Gene by Angel Zarain-Herzberg, Georgina Alvarez-Fernandez

“…Upstream from position -1.5 kb, there is no significant homology among the SERCA2 genes cloned. In addition, the human gene has several repeated sequences mainly of the Alu and L2 type located upstream from position -1.7 kb, spanning in a continuous fashion for more than 40 kb. …”
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