“TRANSHUMANIZATION” OF MODERN EDUCATION by G. L. ILYIN

“…NBICS combine nanotechnology, biotechnology, IT, cognitive and social sciences, genetic engineering, stem cells, and cloning. These research areas converge and create a cumulative effect. …”
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Molecular dynamics simulation based prediction of T-cell epitopes for the production of effector molecules for liver cancer immunotherapy. by Sidra Zafar, Yuhe Bai, Syed Aun Muhammad, Jinlei Guo, Haris Khurram, Saba Zafar, Iraj Muqaddas, Rehan Sadiq Shaikh, Baogang Bai

“…A polyvalent construct of these epitopes was designed using suitable linkers and adjuvant indicated significant binding energy (>-10.5 kcal/mol) with MHC class-I and II molecule. Based on in silico cloning, we found the considerable compatibility of this polyvalent construct with the E. coli expression system and the efficiency of its translation in host. …”
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Development of a multi-epitope chimeric vaccine in silico against Babesia bovis, Theileria annulata, and Anaplasma marginale using computational biology tools and reverse vaccinolo... by Amam Zonaed Siddiki, Sabreena Alam, Farhan Fuad Bin Hossen, Md Abdul Alim

“…The best-designed chimeric vaccine construct was then reverse transcribed and adapted for the host E. coli K12 strain which was later inserted into the pET28a (+) vector for the cloning and expression of the vaccine. The study could be a good initiative for the development of an effective chimeric vaccine against bovine parasites.…”
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Straightforward Procedure for Laboratory Production of DNA Ladder by Vo Thi Thuong Lan, Pham Thi Thanh Loan, Pham Anh Thuy Duong, Le Thi Thanh, Ngo Thi Ha, Ta Bich Thuan

“…This protocol is a combination of routinely employed methods: cloning, PCR, and partial digestion with restriction enzymes. …”
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A Quantitative Risk Evaluation Model for Network Security Based on Body Temperature by Y. P. Jiang, C. C. Cao, X. Mei, H. Guo

“…And the mechanism of increased antibody concentration, which is made up of activating mature detector and cloning memory detector, is mainly used to assess network risk caused by various species of attacks. …”
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Mapping of Novel QTL Regulating Grain Shattering Using Doubled Haploid Population in Rice (Oryza sativa L.) by Gyu-Ho Lee, In-Kyu Kang, Kyung-Min Kim

“…We decide to name this QTL qSh6 and the candidate genes in the qSh6 region can be employed usefully in further research for cloning.…”
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Advance in the Role of Epigenetic Reprogramming in Somatic Cell Nuclear Transfer-Mediated Embryonic Development by Xiaolei Zhang, Shaorong Gao, Xiaoyu Liu

“…However, the low efficiency of the SCNT-mediated reprogramming and the defects of extraembryonic tissues as well as abnormalities of cloned individuals limit the application of reproductive cloning on animals. …”
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Identification of Mutations that Encode Drug Resistance in the Polymerase Gene of the Human Immunodeficiency Virus by Zhengxian Gu, Hengsheng Fang, Horacio Salomon, Qing Gao, Mark A Wainberg

“…The complete reverse transcriptase open reading frames of these viruses, and portions of flanking protease and integrase within the pol gene, were cloned and sequenced by polymerase chain reaction (PCR) techniques. …”
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Divergent Cotton leaf curl Multan betasatellite and three different alphasatellite species associated with cotton leaf curl disease outbreak in Northwest India. by Kajal Kumar Biswas, Nenavath Balram, Marimuthu Elangovan, Supratik Palchoudhury, Utpal Kumar Bhattacharyya, Halima Khatoon, Shilpi Aggarwal, Shruti Godara, Pradeep Kumar, Satish Kumar Sain, Rupesh Arora, Sibnarayan Datta

“…Total plant DNAs were isolated, subjected to rolling circle amplification (RCA), cloning and sequencing. Full-length genome of 12 betasatellites and 13 alphasatellites, those were obtained in the present study, were analyzed. …”
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A dual expression plasmid with Microcin B17 compatible with both prokaryotic and mammalian systems by Agnieszka M. Murakami, Katsuhiro Nagatomo, Hiroshi Koda, Yasutaka Niwa, Manabu Murakami

“…The insertion of the α-peptide gene from lacZ (α-complementation) demonstrated highly efficient cloning of external DNA in the pgMAX-II/MccB17 plasmid. …”
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Beyond Fat Mass: Exploring the Role of Adipokines in Rheumatic Diseases by Morena Scotece, Javier Conde, Rodolfo Gómez, Veronica López, Francisca Lago, Juan Jesus Gómez-Reino, Oreste Gualillo

“…The cloning of leptin in 1994 by Zhang et al. introduced a novel concept about white adipose tissue (WAT) as a very dynamic organ that releases a plethora of immune and inflammatory mediators, such as adipokines and cytokines, which are involved in multiple diseases. …”
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Peculiar Evolutionary History of miR390-Guided TAS3-Like Genes in Land Plants by Maria S. Krasnikova, Denis V. Goryunov, Alexey V. Troitsky, Andrey G. Solovyev, Lydmila V. Ozerova, Sergey Y. Morozov

“…We found relatives of all Physcomitrella patens miR390 and TAS3-like loci in these plant taxa excluding Sphagnopsida. Importantly, cloning and sequencing of Marchantia polymorpha genomic DNA showed miR390 and TAS3-like sequences which were also found among genomic reads of M. polymorpha at NCBI database. …”
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Characterization of the algC Gene Expression Pattern in the Multidrug Resistant Acinetobacter baumannii AIIMS 7 and Correlation with Biofilm Development on Abiotic Surface by Praveen K. Sahu, Pavithra S. Iyer, Sagar H. Barage, Kailas D. Sonawane, Balu A. Chopade

“…Expression levels strongly correlated with specific biofilm stages (scale of 3 to 96 h), coinciding maximum at initial surface attachment stage (9 h) and biofilm maturation stage (48 h). Cloning, heterologous expression, and bioinformatics analyses indicated algC gene product as the bifunctional enzyme phosphomannomutase/phosphoglucomutase (PMM/PGM) of ∼53 kDa size, which augmented biofilms significantly in algC clones compared to controls (lacking algC gene), further localized by scanning electron microscopy. …”
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Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis by Andrés Vacas, Conor Sugden, Óscar Velasco-Rodriguez, Miriam Algarabel-Olona, José Peña-Guerrero, Esther Larrea, Celia Fernández-Rubio, Paul A. Nguewa

“…We have constructed two plasmids expressing the red fluorescent protein mCherry with multiple cloning sites (MCS), adequate for N- and C-terminal fusion protein constructs. …”
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Application of reproductive technologies to the improvement of dairy cattle genomic selection by N. S. Yudin, K. I. Lukyanov, M. I. Voevoda, N. A. Kolchanov

“…Genomic selection can be even more profitable in combination with up-to-date reproductive biotechnologies: semen sexing, multiple ovulation and embryo transfer, ovum pick-up followed by in vitro fertilization, embryo genotyping, cloning of best breeders, etc. In programs of dairy cattle genomic selection, biotechnological procedures with gametes and embryos allow improvement of a variety of parameters determining breeding efficacy: selection intensity, accurate breeding value assessment, and generation interval. …”
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Harnessing marine bacteria for next-gen antibiotics: potent inhibition of S. aureus and Riemerella anatipestifer through in vitro, omics, and chemoinformatics approach with enhance... by Syed Shams ul Hassan, Huizi Jin

“…To investigate the anti-bacterial mechanism, all compounds were docked with two enzymes and TLR4 protein for predicting the vaccine construct, and the best docking score was achieved against RMFP with the highest score of -12.9 for compound 4. In silico cloning was carried out to ensure the expression of proteins generated and were cloned using E. coli as a host. …”
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Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production by Duck Gyun Kim, Boncheol Gu, Yujin Cha, Jeonghan Ha, Yongjae Lee, Gahyeon Kim, Byung-Kwan Cho, Min-Kyu Oh

“…Cas9-BD is also used to capture large BGCs using a developed in vivo cloning method. The modified CRISPR-Cas9 system is successfully applied to many Streptomyces sp., providing versatile and efficient genome editing tools for strain engineering of actinomycetes with high GC content genome.…”
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Protocol to Retrieve Unknown Flanking DNA Using Fork PCR for Genome Walking by Hongjing Wu, Hao Pan, Haixing Li

“…The worth of genome walking includes but is not limited to cloning full-length genes, mining new genes, and discovering regulatory regions of genes. …”
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Molecular Identification of Fungal Communities in a Soil Cultivated with Vegetables and Soil Suppressiveness to Rhizoctonia solani by Silvana Pompéia Val-Moraes, Eliamar Aparecida Nascimbem Pedrinho, Eliana Gertrudes Macedo Lemos, Lucia Maria Carareto-Alves

“…Of the sequenced clones, 22 were obtained from the SMS sample, 18 from the SHC sample, and 6 from the STC sample. …”
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Effect of cutting position in the mother plant and use of auxin on the rooting efficiency of cuttings in four varieties of Cannabis sp. by Axel Voisin, Sebastián Gómez, Esteban Colmann Lerner, Oswaldo Aranda Mosquera, Blanca Villarreal, Sandra Sharry, Christian Weber

“…This work addresses the importance of producing genetically stable plant material, especially in medicinal production where cloning plants with known characteristics is essential for large-scale production. …”
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