Proficiency testing and cross-laboratory method comparison to support standardisation of diatom DNA metabarcoding for freshwater biomonitoring by Valentin Vasselon, Sinziana F. Rivera, Éva Ács, Salomé Baja Almeida, Karl B. Andree, Laure Apothéloz-Perret-Gentil, Bonnie Bailet, Ana Baričević, Kevin K. Beentjes, Juliane Bettig, Agnès Bouchez, Camilla Capelli, Cécile Chardon, Mónika Duleba, Tina Elersek, Clémence Genthon, Maša Jablonska, Louis Jacas, Maria Kahlert, Martyn G. Kelly, Jan-Niklas Macher, Federica Mauri, Marina Moletta-Denat, Andreia Mortágua, Jan Pawlowski, Javier Pérez-Burillo, Martin Pfannkuchen, Erik Pilgrim, Panayiota Pissaridou, Frédéric Rimet, Karmen Stanic, Kálmán Tapolczai, Susanna Theroux, Rosa Trobajo, Berry Van der Hoorn, Marlen I. Vasquez, Marie Vidal, David Wanless, Jonathan Warren, Jonas Zimmermann, Benoît Paix

“…Experiments were performed by each participant on a set of standardised DNA and biofilm samples (river, lake and mock community) to investigate potential systematic and random errors. …”
Get full text

Cooperation Between <i>Rhodococcus qinshengii</i> and <i>Rhodococcus erythropolis</i> for Carbendazim Degradation by Roosivelt Solano-Rodríguez, Fortunata Santoyo-Tepole, Mario Figueroa, Voleta Larios-Serrato, Nora Ruiz-Ordaz, Abigail Pérez-Valdespino, Everardo Curiel-Quesada

“…In this work, <i>Rhodococcus qingshengii</i> RC1 and <i>Rhodococcus erythropolis</i> RC9 were isolated from a bacterial community growing in a biofilm reactor acclimated with microbiota from carbendazim-contaminated soil. …”
Get full text

Microbial community structure and functional traits involved in the adaptation of culturable bacteria within the gut of amphipods from the deepest ocean by Yukun Cui, Yu Xiao, Zhuo Wang, Paiyao Ji, Changhao Zhang, Yongqi Li, Jiasong Fang, Xi Yu

“…In addition, the presence of antibacterial compounds and antibiotic resistance genes, as well as the ability to form a biofilm, facilitated the successful colonization of P. pentosaceus XY62 in the gut environment.IMPORTANCEAmphipods are widely distributed in the Hadal trenches, and the study of their gut microbes has garnered considerable scientific interest. …”
Get full text

Development of monoclonal antibodies against P. gingivalis Mfa1 and their protective capacity in an experimental periodontitis model by Mingya Cao, Siyu Wang, Shengke Zhou, Min Yan, Yu Zou, Yuan Cui, Xinyu Lou, Yichang Gao, Ying Chen, Zijing Han, Yi Qian, Jingying Chen, Xia Li

“…Fimbriae protein Mfa1 is a key virulence factor of P. gingivalis and plays a crucial role in bacterial adhesion, colonization, biofilm formation, and persistent inflammation, making it a promising therapeutic target. …”
Get full text

Identification of microbial exopolymer producers in sandy and muddy intertidal sediments by compound-specific isotope analysis by Hubas, Cédric, Gaubert-Boussarie, Julie, D’Hondt, An-Sofie, Jesus, Bruno, Lamy, Dominique, Meleder, Vona, Prins, Antoine, Rosa, Philippe, Stock, Willem, Sabbe, Koen

“…Extracellular polymeric substances (EPS) refer to a wide variety of high molecular weight molecules secreted outside the cell membrane by biofilm microorganisms. In the present study, EPS from marine microphytobenthic biofilms were extracted and their isotope ratios were analysed. …”
Get full text

Sulforaphane as a promising anti-caries agents: inhibitory effects on Streptococcus mutans and caries control in a rat model by Meijiao Yu, Meijiao Yu, Yu Chen, Yu Chen, Sishi Dong, Sishi Dong, Zhongxin Chen, Zhongxin Chen, Xuelian Jiang, Xuelian Jiang, Yufei Wang, Yufei Wang, Linglin Zhang, Linglin Zhang

“…The growth of S. mutans and the biofilm formation were inhibited by SFN in a dose-dependent manner through suppressing the synthesis of extracellular polysaccharide (EPS) and acid production, as well as decreasing the acid tolerance. …”
Get full text

Multidrug-resistant ESBL E. coli in urban surface waters and public health implications: A Case Study from Goranchatbari, Dhaka by Md. Sakib Hossain, Ahmed Ishtiaque Amin Chowdhury, Mohammad Rafiqul Islam, Ripan Kirtunia, Md. Foysal Abedin, Mohammad Atique Ul Alam, Shampa, Sonia Binte Murshed, Md Shadman Sakib, Siam Alam, M. Shahjahan Mondal, Zahid Hayat Mahmud

“…Isolates of ESBL E. coli underwent analysis for the major ESBL and pathogenic genes, antibiotic susceptibility, biofilm formation, and genetic diversity. However, the existence of E. coli was confirmed in every sample. …”
Get full text

Technological dental sealants: in vitro evaluation of material properties and antibiofilm potential by Witalo Pereira de Jesus, Luiza de Almeida Queiroz Ferreira, Washington Henrique Themoteo da Silva, Fernanda Vieira Belém, Ana Paula Turrioni, Cláudia Silami de Magalhães, Mila Fernandes Moreira Madeira, Ivana Márcia Alves Diniz, Marco Aurelio Benini Paschoal

“…For the MAS test, a bacterial biofilm of S. mutans was induced for five days in BHI broth + 5% sucrose and measurement of CFU/ml (log 10 from serial dilutions). …”
Get full text

Emerging Resistance and Virulence Patterns in <i>Salmonella enterica</i>: Insights into Silver Nanoparticles as an Antimicrobial Strategy by Irina Gheorghe-Barbu, Ilda Czobor Barbu, Rareș-Ionuț Dragomir, Ioana Cristina Marinaș, Miruna Silvia Stan, Radu Pericleanu, Andreea Ștefania Dumbravă, Liviu-Iulian Rotaru, Simona Paraschiv, Leontina Mirela Bănică, Ionuț Pecete, Dan Oțelea, Violeta Corina Cristea, Mircea Ioan Popa, Marilena Monica Țânțu, Marius Surleac

“…Methods: In 2019, community-acquired <i>Salmonella</i> strains were collected and identified using MALDI-TOF mass spectrometry, antibiotic susceptibility profiles have been determined with the MicroScan system, and soluble virulence factors were evaluated using specific culture media, while biofilm formation was quantified in 96-well plates. …”
Get full text

Alleviating <i>Clostridium perfringens</i>-Induced Intestinal Lesions in Chickens Using the Xylanase <i>Cb</i>Xyn10C and Its Binary Cocktail with a Protease by Wenjing Zhang, Zhenzhen Hao, Daoxin Yang, Wangli Ji, Kairui Guo, Xianhua Sun, Shuai Wang, Shuyan Yang, Jianshuang Ma, Tong Wang, Huiying Luo, Bin Yao, Meiling Zhang, Yuan Wang, Huoqing Huang, Xiaoyun Su

“…In this study, a xylanase (<i>Cb</i>Xyn10C), which effectively promotes the growth of probiotics, and a protease, which degrades the biofilm of <i>C. perfringens,</i> were analyzed for their ability to alleviate <i>C. perfringens</i>-induced necrotic enteritis in broiler chickens. …”
Get full text

Application of trichloroisocyanuric acid in controlling kiwifruit bacterial canker disease demonstrates its promising potential as an eco-friendly bactericide by Jianyou Gao, Jiewei Li, Cuixia Liu, Hongjuan Gong, Beibei Qi, Rongxiang Zhu, Liming Xia, Li Li, Shibiao Liu, Qiaosheng Jiang, Kaiyu Ye, Faming Wang

“…Mechanistic investigations revealed that TCCA inhibited bacterial biofilm formation, impaired motility, disrupted cell integrity, and suppressed the expression of virulence-related genes, ultimately leading to bacterial cell death. …”
Get full text

Confocal Microscopy for Detection of Intracellular Bacteria in Urine Samples of patients Suspected with Urinary Tract Infection: A Cross-sectional Observational Study by Bhagyashri Patil-Takbhate, Nilam Memane, Srikanth Tripathy, Nageswari Gandham, Shahzad Mirza

“…Urine samples showing intracellular gram-negative bacteria by Gram staining were further processed for fluorescence confocal microscopy followed by conventional culture and virulence detection by multiplex Polymerase Chain Reaction (PCR). Formation of biofilm ability was assessed by tube method. Quantitative data presented as mean and Standard Deviation (SD) while qualitative variables expressed as frequency (percentage). …”
Get full text

Autoinducer-2 enhances the defense of Vibrio furnissii against oxidative stress and DNA damage by modulation of c-di-GMP signaling via a two-component system by Heng Zhang, Wenjin Zhao, Wenguang Yang, Huimin Zhang, Xinyu Qian, Kai Sun, Qiao Yang, Xihui Shen, Lei Zhang

“…In contrast with another well-known AI-2 receptor LuxP that recognizes the borated form of AI-2, AsrK is found to show higher affinity with AI-2 under borate-depleted conditions, and thus boron has a negative effect on AI-2 sensing by AsrK in regulation of the biofilm and motility phenotypes. AI-2 binds to the extracytoplasmic dCache_1 domain of AsrK to inhibit its autokinase activity, thus decreasing the phosphorylation level of its cognate response regulator AsrR and activating the phosphodiesterase activity of AsrR to degrade the cellular second messenger cyclic di-GMP (c-di-GMP). …”
Get full text

Newly identified c-di-GMP pathway putative EAL domain gene STM0343 regulates stress resistance and virulence in Salmonella enterica serovar Typhimurium by Kaifeng Chen, Lili Li, Nanwei Wang, Zhouping Zhou, Peng Pan, Chenggang Xu, Dage Sun, Jiayi Li, Changzhi Dai, Dai Kuang, Ming Liao, Jianmin Zhang

“…Specifically, it exhibited increases of 95.74% in extracellular protein and 35.96% in exopolysaccharide production by upregulating the expression of relevant genes. As a result, the biofilm formation ability of 269ΔSTM0343 was enhanced by 21.54%, accompanied by a more pronounced red, dry, and rough colony morphology. 269ΔSTM0343 also showed a 19.03% decrease in motility due to the downregulation of flhD expression. …”
Get full text

Synthesis and Antibacterial and Antibiofilm Activity of Iron Oxide Glycerol Nanoparticles Obtained by Coprecipitation Method by Simona Liliana Iconaru, Alina Mihaela Prodan, Philippe Le Coustumer, Daniela Predoi

“…The results indicate that, in the case of P. aeruginosa  1397 biofilms, at concentrations from 0.01 mg/mL to 0.625 mg/mL, the glycerol iron oxide inhibits the ability of this strain to develop biofilms on the inert substratum.…”
Get full text

Megacities as Sources for Pathogenic Bacteria in Rivers and Their Fate Downstream by Wolf-Rainer Abraham

“…They also facilitate gene transfers of antibiotic resistance genes which become an increasing health problem. Beside biofilms, amoebae are carriers of pathogenic bacteria and niches for their survival. …”
Get full text

Revisiting the Synergistic In Vitro Antimicrobial and Antibiofilm Potential of Chlorhexidine Gluconate and Cetrimide in Combination as an Antiseptic and Disinfectant Agent by Diamond Jain, Rimjhim Gupta, Rashmi Mehta, Pratheesh N. Prabhakaran, Deva Kumari, Kulpreet Bhui, Deepa Murali

“…Further, the effects on the biofilms were visualized using brightfield and confocal laser scanning microscopy. …”
Get full text

Benefits of Immobilized Bacteria in Bioremediation of Sites Contaminated with Toxic Organic Compounds by Emanuel Gheorghita Armanu, Simone Bertoldi, Łukasz Chrzanowski, Irina Volf, Hermann J. Heipieper, Christian Eberlein

“…A physical matrix is essential for the formation, maintenance, and survival of bacterial biofilms. By providing such a matrix for bacterial immobilization, the formation of biofilms can be facilitated and accelerated. …”
Get full text

Microbe-assisted fabrication of circularly polarized luminescent bacterial cellulosic hybrids by Yongjie Sun, Dan Zhang, Zhiqiang Dong, Jinxiao Lyu, Chunfei Wang, Jun Gong, Koon Ho Wong, Changfeng Wu, Xuanjun Zhang

“…Enlighted by microbe-assisted asymmetric biosynthesis, herein, we show the in situ bacterial fermentation of Komagataeibacter sucrofermentants to fabricate a series of bacterial cellulosic biofilms with CPL of green, orange, red, and near-infrared colors. …”
Get full text

The Macrophage Inflammatory Proteins MIP1α (CCL3) and MIP2α (CXCL2) in Implant-Associated Osteomyelitis: Linking Inflammation to Bone Degradation by Ulrike Dapunt, Susanne Maurer, Thomas Giese, Matthias Martin Gaida, Gertrud Maria Hänsch

“…These infections are difficult to treat, because many bacterial species form biofilms on implants, which are relatively resistant towards antibiotics. …”
Get full text