Myocarditis and neutrophil-mediated vascular leakage but not cytokine storm associated with fatal murine leptospirosisResearch in context by Stylianos Papadopoulos, David Hardy, Frédérique Vernel-Pauillac, Magali Tichit, Ivo G. Boneca, Catherine Werts

“…On day 3 post infection, the mice were humanely euthanised and blood and organs were collected. Bacterial load, biochemical parameters, cytokine production and leucocyte population were assessed by qPCR, ELISA, cytometry and immunohistochemistry. …”
Get full text

Targeted allele-specific FGFR2 knockdown via human recombinant ferritin nanoparticles for personalized treatment of Crouzon syndrome by Federica Tiberio, Martina Salvati, Luca Polito, Giada Tisci, Alessia Vita, Ornella Parolini, Luca Massimi, Lorena Di Pietro, Pierpaolo Ceci, Gianpiero Tamburrini, Alessandro Arcovito, Elisabetta Falvo, Wanda Lattanzi

Get full text

APOE Christchurch enhances a disease-associated microglial response to plaque but suppresses response to tau pathology by Kristine M. Tran, Nellie E. Kwang, Claire A. Butler, Angela Gomez-Arboledas, Shimako Kawauchi, Cassandra Mar, Donna Chao, Rocio A. Barahona, Celia Da Cunha, Kate I. Tsourmas, Zechuan Shi, Shuling Wang, Sherilyn Collins, Amber Walker, Kai-Xuan Shi, Joshua A. Alcantara, Jonathan Neumann, Duc M. Duong, Nicholas T. Seyfried, Andrea J. Tenner, Frank M. LaFerla, Lindsay A. Hohsfield, Vivek Swarup, Grant R. MacGregor, Kim N. Green

“…We used immunohistochemical and biochemical analysis along with single-cell spatial omics and bulk proteomics to explore the impact of the ApoeCh variant on AD pathological development and the brain’s response to plaques and tau. …”
Get full text

Effects of <i>Enterococcus faecalis</i> Supplementation on Growth Performance, Hepatic Lipid Metabolism, and mRNA Expression of Lipid Metabolism Genes and Intestinal Flora in Geese by Siyu Sun, Yujie Zhao, Zhen Pang, Baoxia Wan, Jiaqi Wang, Zhenyu Wu, Qiuju Wang

“…The 16S rRNA data demonstrated a strong correlation between microbial communities and lipid-related physiological and biochemical indicators (<i>p</i> < 0.05). In conclusion, <i>E. faecalis</i> supplementation promoted fatty acid oxidation, reduced blood lipid levels, alleviated hepatic lipid accumulation, and improved ileal morphology and intestinal flora diversity, thereby enhancing growth performance and lipid metabolism in geese. …”
Get full text

European Guideline on IgG4-related Digestive Disease — UEG and SGF Evidence-based Recommendations by J.-M. Lohr, U. Beuers, M. Vujasinovic, D. Alvaro, J. B. Frøkjær, F. Buttgereit, G. Capurso, E. L. Culver, E. de-Madaria, E. Della-Torre, S. Detlefsen, E. Dominguez-Munoz, P. Czubkowski, N. Ewald, L. Frulloni, N. Gubergrits, D. G. Duman, Th. Hackert, J. Iglesias-Garcia, N. Kartalis, A. Laghi, F. Lammert, F. Lindgren, A. Okhlobystin, G. Oracz, A. Parniczky, R. M. P. Mucelli, V. Rebours, J. Rosendahl, N. Schleinitz, A. Schneider, E. FH van Bommel, C. S. Verbeke, M. P. Vullierme, H. Witt, the UEG guideline working group

“…Response to initial treatment should be assessed at week 2-4 with clinical, biochemical and morphological markers. Maintenance treatment with glucocorticoids should be considered in multi-organ disease or history of relapse. …”
Get full text

The effect of using red algae powder on growth indices, some serum and mucus indices, and mucus protein patterns in common carp (Cyprinus carpio) fry by Fatemeh Abdollahnejad, Seyed Hossein Hoseinifar, Hamed Paknejad, Abdolmajid Hajimoradlou, Mohamdreza Imanpuor

“…Studies have shown that the use of dried algae as an immune stimulant in the aquaculture industry improves the physiological responses of fish to stress and disease, and as an immune stimulant in aquaculture, they increase growth, food efficiency, and biochemical quality of carcasses in various aquatic animals. …”
Get full text

Growth, fat metabolism and hepatic health in largemouth bass fed varying fat-level diets by Digen Qin, Jiale Chen, Junxiong Li, Zhiyou Liu, Weibin Huang, Yuanzhi Yang, Jing Zhang, Beiping Tan, Xiaohui Dong

“…The contents of fat and ash in whole-body, and hepatic fat contents were increased with dietary fat levels (P<0.05), the contents of moisture and crude protein in whole-body and liver exhibited no substantial variations (P>0.05). Serum biochemical indexes and serum alanine aminotransferase (ALT) activity, liver glutathione peroxidase (GSH-Px) and catalase (CAT) activities were increased significantly with rising dietary fat levels (P<0.05), serum aspartate transaminase (AST) and liver superoxide dismutase (SOD) activities had no statistical differences (P>0.05). …”
Get full text

Chemogenomic Screening in a Patient‐Derived 3D Fatty Liver Disease Model Reveals the CHRM1‐TRPM8 Axis as a Novel Module for Targeted Intervention by Sonia Youhanna, Aurino M. Kemas, Shane C. Wright, Yi Zhong, Britta Klumpp, Kathrin Klein, Aikaterini Motso, Maurice Michel, Nicole Ziegler, Mingmei Shang, Pierre Sabatier, Aimo Kannt, Hongda Sheng, Nuria Oliva‐Vilarnau, Florian A. Büttner, Brinton Seashore‐Ludlow, Jonas Schreiner, Maike Windbergs, Martin Cornillet, Niklas K. Björkström, Andreas J. Hülsmeier, Thorsten Hornemann, Jesper V. Olsen, Yi Wang, Roberto Gramignoli, Michael Sundström, Volker M. Lauschke

“…By combining high‐content imaging with scalable biochemical assays and chemogenomic screening, multiple novel targets with anti‐steatotic, anti‐inflammatory, and anti‐fibrotic effects are identified. …”
Get full text

Targeted ErbB4 receptor activation prevents D-galactose-induced neuronal senescence via inhibiting ferroptosis pathway by Ji-Ji Dao, Ji-Ji Dao, Wei Zhang, Wei Zhang, Chong Liu, Chong Liu, Qian Li, Qian Li, Chen-Meng Qiao, Chen-Meng Qiao, Chun Cui, Chun Cui, Yan-Qin Shen, Yan-Qin Shen, Shuang-Xi Chen, Shuang-Xi Chen, Wei-Jiang Zhao, Wei-Jiang Zhao

“…Immunofluorescence staining, western blot, and biochemical assays were primarily employed to assess changes in both senescence- and ferroptosis-related molecules in mouse hippocampal tissues in response to each treatment. …”
Get full text

N-acetylcysteine increased the growth performance of Leuciscus waleckii by improving mitochondrial function, enhancing antioxidant and anti-inflammatory, and inhibiting apoptosis i... by Deng Lai Li, Rui Zhu, Si Tong Meng, Yin Tao Wang, Si Ying Liu, Zhi Yong Yang, Li Fang Wu

“…The results revealed that 3 g/kg-6 g/kg NAC increased FW, WGR, SGR, PER, and FER and improved growth performance and feed utilization in Leuciscus waleckii. 4.50 g/kg-6.00 g/kg NAC increased the intestinal and hepatopancreatic digestive enzyme activities, enhancing digestive function. 3.00 g/kg-4.50 g/kg NAC increased serum ACP and AKP activities, decreased IL-1β and TNF-α levels, and optimized serum biochemical indices in Leuciscus waleckii. Appropriate level of NAC upregulated mRNA expression of PPARγ, ATGL and CPT-1, and HDL-C, CPT-1, ATGL and FAS activities to promote lipid metabolism. 3.00 g/kg-6.00 g/kg NAC promotes hepatopancreatic and intestinal anti-inflammatory and antioxidant capacities by activating Nrf2 and inhibiting the NF-κB signaling pathway. …”
Get full text

Gender Disparity in the Relationship between Prevalence of Thyroid Nodules and Metabolic Syndrome Components: The SHDC-CDPC Community-Based Study by Xiaoying Ding, Ying Xu, Yufan Wang, Xiaohua Li, Chunhua Lu, Jing Su, Yuting Chen, Yuhang Ma, Yanhua Yin, Yong Wu, Yaqiong Jin, Lihua Yu, Junyi Jiang, Naisi Zhao, Qingwu Yan, Andrew S. Greenberg, Haiyan Sun, Mingyu Gu, Li Zhao, Yunhong Huang, Yijie Wu, Chunxian Qian, Yongde Peng

“…A total of 6,798 subjects, including 2201 patients with TN, were enrolled in this study. Anthropometric, biochemical, thyroid ultrasonographic, and other metabolic parameters were all measured. …”
Get full text

The Combined Effect of Garlic (Allium sativum) and Turmeric (Curcuma longa) Extracts to Reduce the Adverse Effects of Aflatoxin B1 in Rats by Hadi Sarir, Saeedeh Zakerian, masood didarkhah

“…At the end of the treatment period, blood samples were collected for biochemical study.Results and Discussion: Daily administration of aflatoxin B1 at a dose of 100 mg/kg body weight for 28 days resulted in increased levels of AST, ALT, ALP, total bilirubin, and malondialdehyde, along with decreased total antioxidant capacity, T3 (triiodothyronine), and T4 (thyroxine). …”
Get full text

Isolation and Characterization of Indigenous <i>Acetobacter</i> Strains from Cashew Apple and Their Potential Use in Vinegar Production by Yaya Anianhou Ouattara, Doudjo Soro, Kouakou Romain Fossou, Ahou Cinthia Ines Yebouet, Ismael Abraham Cissé, Dogbo Marius Akissi, Adolphe Zézé, Emmanuel Assidjo, Kouassi Benjamin Yao

“…Vinegar mother from fermented juice was used to isolate acetic acid bacteria on a standard glucose-based medium. Physiological and biochemical tests followed by 16S rRNA gene analysis and phylogeny were used for isolate characterization. …”
Get full text

Biallelic SLC13A1 loss-of-function variants result in impaired sulfate transport and skeletal phenotypes, including short stature, scoliosis, and skeletal dysplasia by Christina G. Tise, Katie Ashton, Lachlan de Hayr, Kun-Di Lee, Omkar L. Patkar, Emma Krzesinski, Jennifer A. Bassetti, Erin M. Carter, Cathleen Raggio, Andreas Zankl, Anas M. Khanshour, Kristhen N. Atala, Jonathan J. Rios, Carol A. Wise, Ying Zhu, Futao Zhang, Tony Roscioli, Michael Buckley, Robert J. Harvey, Paul A. Dawson

“…In this study, we characterized the clinical, biochemical, and functional impact of biallelic SLC13A1 nonsense and/or missense variants in individuals presenting with a skeletal phenotype. …”
Get full text

Prevalence and Antimicrobial Susceptibility of Bacterial Uropathogens Isolated from Pediatric Patients at Yekatit 12 Hospital Medical College, Addis Ababa, Ethiopia by Yamirot Merga Duffa, Kumera Terfa Kitila, Dereje Mamuye Gebretsadik, Adane Bitew

“…Bacterial isolates were indentified and characterized by Gram stain and by using an array of standard routine biochemical tests. The antimicrobial susceptibility test was carried out by using the Kirby–Bauer disc diffusion method. …”
Get full text

Effects of dietary metabolizable energy and crude protein levels on production performance, meat quality and cecal microbiota of Taihe Silky Fowl during growing period by Yutian Shen, Wentao Li, Lixia Kai, Yuqing Fan, Youping Wu, Fengqin Wang, Yizhen Wang, Zeqing Lu

“…Dietary CP level and the interaction between ME and CP had no significant effects on growth performance, slaughter performance, organ index, serum biochemical indexes and meat quality of Taihe Silky Fowl during growing period. …”
Get full text

Diagnosis and treatment of the irritable bowel syndrome: clinical guidelines of the Russian gastroenterological association and Russian association of coloproctology by V. T. Ivashkin, Yu. A. Shelygin, Ye. K. Baranskaya, Ye. A. Belousova, A. G. Beniashvili, S. V. Vasilyev, A. O. Golovenko, O. V. Golovenko, Ye. G. Grigoryev, N. V. Kostenko, T. L. Lapina, I. D. Loranskaya, O. S. Lyashenko, I. V. Mayev, Ye. A. Poluektova, V. G. Rumyantsev, V. M. Timerbulatov, A. S. Trukhmanov, O. Yu. Fomenko, I. L. Khalif, E. Yu. Chashkova, A. A. Sheptulin, O. S. Shifrin, V. V. Yanovoy

“…Due to complexity of differential diagnostics, IBS should be considered as diagnosis by exclusion that require following investigations: clinical and biochemical blood tests; IgA or IgG anti-tissue transglutaminase antibodies; thyroid hormone levels; fecal occult blood test; glucose or lactulose hydrogen breath test for bacterial overgrowth syndrome; stool test for coliform bacteria, stool test for Clostridium difficile toxins A and B; fecal calprotectin level; abdominal ultrasound; upper and lower endoscopies, at indications - with biopsies. …”
Get full text

Investigating the contribution of understory to radiative transfer simulations through reconstructing 3-D realistic temperate broadleaf forest scenes based on multi-platform laser... by Xiaohan Lin, Ainong Li, Jinhu Bian, Zhengjian Zhang, Xi Nan, Limin Chen, Yi Bai, Yi Deng, Siyuan Li

“…This study provides a successful case for modeling the multi-layered forest structure in natural temperate broadleaf forests, and even offers a theoretical reference for facilitating the retrieval of biochemical and biophysical information from the understory by remote sensing.…”
Get full text

Dynamic conditioning of porcine kidney grafts with extracellular vesicles derived from urine progenitor cells: A proof‐of‐concept study by Perrine Burdeyron, Sébastien Giraud, Maryne Lepoittevin, Nina Jordan, Sonia Brishoual, Maïté Jacquard, Virginie Ameteau, Nadège Boildieu, Estelle Lemarie, Jonathan Daniel, Frédéric Martins, Nicolas Mélis, Marine Coué, Raphaël Thuillier, Henri Leuvenink, Luc Pellerin, Thierry Hauet, Clara Steichen

“…·pUPC‐EVs‐treated kidneys have distinct biochemical, metabolic, and transcriptomic profiles highlighting targets of interest. …”
Get full text

Variation in seed traits among different eelgrass populations in China by Mingjie Liu, Xinhua Wang, Xiaochen Liu, Yu Zhang, Shidong Yue, Xiaomei Zhang, Shaochun Xu, Yi Zhou

“…In this study, the seed morphology, germination, and biochemical composition of different eelgrass populations in China were investigated. …”
Get full text