Scaffold-enabled high-resolution cryo-EM structure determination of RNA by Daniel B. Haack, Boris Rudolfs, Shouhong Jin, Alexandra Khitun, Kevin M. Weeks, Navtej Toor

“…In the case of the TPP riboswitch aptamer domain, the scaffolding approach allowed visualization of the riboswitch ligand binding pocket at 2.5 Å resolution. …”
Get full text

A Sensitive and Selective Electrochemical Aptasensor for Carbendazim Detection by Suthira Pushparajah, Mahnaz Shafiei, Aimin Yu

“…Then, a CBZ-specific aptamer was attached via Pt-sulfur bonds. Upon combining CBZ with the aptamer on the electrode surface, the redox reaction of the electrochemical probe K₄[Fe(CN)₆] is hindered, resulting in a current drop. …”
Get full text

Extraordinary tunable dynamic range of electrochemical aptasensor for accurate detection of ochratoxin A in food samples by Lin Cheng, Hao Qu, Jun Teng, Li Yao, Feng Xue, Wei Chen

“…This electrochemical aptasensor is constructed based on the target induced aptamer-folding detection mechanism and the recognition between OTA and its aptamers results in the conformational change of the aptamer probe and thus signal changes for measurement. …”
Get full text

RNA folding kinetics control riboswitch sensitivity in vivo by David Z. Bushhouse, Jiayu Fu, Julius B. Lucks

“…Understanding how riboswitch sensitivity to ligand (EC50) is controlled is critical to explain how highly conserved aptamer domains are deployed in a variety of contexts with different sensitivity demands. …”
Get full text

ATLAS-seq: a microfluidic single-cell TCR screen for antigen-reactive TCRs by Siwei Luo, Amber Notaro, Lan Lin

“…Here, we develop a microfluidic single-cell screening method for antigen-reactive T cells named ATLAS-seq (Aptamer-based T Lymphocyte Activity Screening and SEQuencing). …”
Get full text

An antifouling polydopamine-based fluorescent aptasensor for determination of arginine kinase by Yanbo Wang, Huan Li, Jinru Zhou, Fangting Wang, Yifan Qian, Linglin Fu

“…With the presence of AK, the PDA-based aptasensor exhibited elevated fluorescent signals as the FAM-labeled aptamer bound to AK and detached from the PDA particles. …”
Get full text

Nanoarchitectonics of Biofunctionalized Hydrogen‐Terminated 2D‐Germanane Heterostructures as Highly Sensitive Biorecognition Transducers: The Case Study of Cocaine Drug by Yiming Lei, Ángel Campos‐Lendínez, Xavier Sala, Jordi García‐Antón, Jose Muñoz

“…Interestingly, the aptamer–cocaine interactions hinder the interfacial electron‐transfer process of the benchmark [Fe(CN)6]3−/4− redox marker with increasing concentration of the cocaine target, leading to a detection limit as low as 4.9 ± 0.1 aM, the lowest one reported in literature by far. …”
Get full text

Preparation, characterisation, and in vitro cancer‐suppression function of RNA nanoparticles carrying miR‐301b‐3p Inhibitor by Junjie Zhao, Niu Niu, Fan Yang, Haitao Liu, Weibo Qi

“…Methods The NPs were composed of miR‐301b‐3p, A549 aptamer (A549apt), and Cyanine 5 in a bottom‐up manner with a 3‐way‐junction (3WJ) structure. …”
Get full text

Tenascin C-Guided Nanosystem for Precision Delivery of Obeticholic Acid in Liver Fibrosis Therapy by Yawen Wang, Lei Yang, Qing Xu, Taiyu Liu, Hongliang He, Lisha Liu, Lifang Yin

“…<b>Methods:</b> We designed a liposomal delivery system functionalized with the GBI-10 aptamer and encapsulating obeticholic acid (OCA lips@Apt) to enhance selective delivery to fibrotic liver tissue while minimizing systemic toxicity. …”
Get full text

Detection of Quadruplex DNA by Gold Nanoparticles by Heather F. Crouse, Alex Doudt, Cassie Zerbe, Swarna Basu

“…Enhancement of PRLS signals greater than 50% was observed at nanomolar DNA concentration, and a lower limit of detection of 2.1 nM was established for three different quadruplex DNA sequences, including the thrombin-inhibiting single-stranded 15 mer aptamer DNA, d(GGTTGGTGTGGTTGG), and the double-stranded 12 mer DNA, d(G4T4G4). …”
Get full text

A critical review of the limitations of current diagnostic techniques for schistosomiasis by Ilemobayo Victor Fasogbon, Erick Nyakundi Ondari, Deusdedit Tusubira, Egwu Chinedu Ogbonnia, Jon Ashley, Swamiappan Sasikumar, Patrick Maduabuchi Aja

“…This review summarizes the published literature on the diagnostics of schistosomiasis based on the techniques of microscopy, serology, molecular, antigen-based and aptamer-based assays. The limitations of each technique were summarized to encourage future research. …”
Get full text

Antivascular Endothelial Growth Factor Agents for Neovascular Age-Related Macular Degeneration by Ilias Zampros, Anna Praidou, Periklis Brazitikos, Panagiotis Ekonomidis, Sofia Androudi

“…Pegaptanib sodium, which is an aptamer and ranibizumab, which is a monoclonal antibody fragment. …”
Get full text

A microscale thermophoresis-based enzymatic RNA methyltransferase assay enables the discovery of DNMT2 inhibitors by Zarina Nidoieva, Mark O. Sabin, Tristan Dewald, Annabelle C. Weldert, Sabrina N. Hoba, Mark Helm, Fabian Barthels

“…This study developed a microscale thermophoresis (MST)-based split aptamer assay for enzymatic MTase investigations, improving current methodologies by offering a non-proprietary, cost-effective, and highly sensitive approach. …”
Get full text

Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites by Antonella Virgilio, Luciano Mayol, Michela Varra, Aldo Galeone, Veronica Esposito

“…Heterochiral oligodeoxynucleotides based on the thrombin binding aptamer sequence, namely, 5′gg3′-3′TT5′-5′ggtgtgg3′-3′TT5′-5′gg3′ (H1), 5′gg3′-3′TT5′-5′gg3′-3′TGT5′-5′gg3′-3′TT5′-5′gg3′ (H2), and 5′gGTTGgtgtgGTTGg3′ (H3), where lower case letters indicate L-residues, have been investigated in their ability to fold in G-quadruplex structures through a combination of gel electrophoresis, circular dichroism, and UV spectroscopy techniques. …”
Get full text

Synthesis of Specifically Modified Oligonucleotides for Application in Structural and Functional Analysis of RNA by Nico Rublack, Hien Nguyen, Bettina Appel, Danilo Springstubbe, Denise Strohbach, Sabine Müller

“…Beyond synthetic RNAs for antisense, aptamer, ribozyme, and siRNA technologies, oligoribonucleotides carrying site-specific modifications for structure and function studies are needed. …”
Get full text

Promising horizons in achondroplasia along with the development of new drugs by Keiichi Ozono, Takuo Kubota, Toshimi Michigami

“…Additionally in development are a tyrosine kinase inhibitor, a soluble FGFR3, an antibody against FGFR3, meclizine, and the FGF2-aptamer. New drugs will bring a brighter future for patients with ACH.…”
Get full text

RNase P cleavage of pseudoknot substrates reveals differences in active site architecture that depend on residue N-1 in the 5’ leader by David M. Kosek, J. Luis Leal, Ema Kikovska-Stojanovska, Guanzhong Mao, Shiying Wu, Samuel C. Flores, Leif A. Kirsebom

“…We show that a small biotin-binding RNA aptamer that folds into a pseudoknot structure acts as a substrate for bacterial RNase P RNA (RPR) with and without the RNase P C5 protein. …”
Get full text

Different electrode system based electrochemical aptasensor for the detection of dengue virus antigen in human serum: A comparative study by Mohd Rahil Hasan, Pradakshina Sharma, Saumitra Singh, Sushil Kumar, Shivani, Kirti Rani, Jagriti Narang

“…Both the electrode types were coated with chemically synthesized ZnO-Nanomaterial, which aids in electron transport, and to make it more selective highly specific DNA-aptamer was used against the DENV antigen. SEM and Uv–Vis spectra approaches were used to characterize the synthesized nanomaterial. …”
Get full text

Detection of Hg2+ Using a Dual-Mode Biosensing Probe Constructed Using Ratiometric Fluorescent Copper Nanoclusters@Zirconia Metal-Organic Framework/N-Methyl Mesoporphyrin IX and Co... by Shikha Jain, Monika Nehra, Neeraj Dilbaghi, Ganga Ram Chaudhary, Sandeep Kumar

“…Moreover, the G-quadruplex (G4) structure of the aptamer enhances the fluorescence emission of NMM (610 nm). …”
Get full text

Colorimetric aptasensor coupled with a deep-learning-powered smartphone app for programmed death ligand-1 expressing extracellular vesicles by Adeel Khan, Adeel Khan, Haroon Khan, Nongyue He, Zhiyang Li, Heba Khalil Alyahya, Yousef A. Bin Jardan

“…Our approach is the development of a colorimetric aptasensor by combining the rapid capturing efficiency of (Fe3O4)-SiO2-TiO2 for EV isolation with PD-L1 aptamer-triggered enzyme-linked hybridization chain reaction (HCR) for signal amplification. …”
Get full text