Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases by Athanasios Mavropoulos, Dimitrios P. Bogdanos, Christos Liaskos, Timoklia Orfanidou, Theodora Simopoulou, Efterpi Zafiriou, Lazaros I. Sakkas, Eirini I. Rigopoulou

“…To overcome these limitations, we have modified methanol-based phosphoflow protocols using several commercially available antibody clones suitable for surface antigens, intracellular cytokines, and p38 MAPK. These included markers of B cells (CD19, CD20, and CD22), T cells (CD3, CD4, and CD8), NK (CD56 and CD7), and dendritic cells (CD11c). …”
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A Model of Insulin Resistance in Mice, Born to Diabetic Pregnancy, Is Associated with Alterations of Transcription-Related Genes in Pancreas and Epididymal Adipose Tissue by Akadiri Yessoufou, Kabirou Moutairou, Naim Akhtar Khan

“…Though IR offspring exhibited reduced epididymal adipose tissue, their adipocytes seemed to be differentiated into macrophage-like cells, as they exhibited upregulated CD14 and CD68 antigens, generally expressed by macrophages. However, there was no peripheral macrophages infiltration into epididymal adipose tissue, as the expression of F4/80, a true macrophage marker, was undetectable. …”
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The Increase of Circulating PD-1- and PD-L1-Expressing Lymphocytes in Endometriosis: Correlation with Clinical and Laboratory Parameters by Monika Walankiewicz, Ewelina Grywalska, Grzegorz Polak, Izabela Korona-Glowniak, Elzbieta Witt, Agata Surdacka, Jan Kotarski, Jacek Rolinski

“…Expression of PD-1 and PD-L1 on T and B cells could represent the hallmark of immune system reaction to chronic antigenic exposition in patients with endometriosis.…”
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Reprogramming of Melanoma Tumor-Infiltrating Lymphocytes to Induced Pluripotent Stem Cells by Hidehito Saito, Keisuke Okita, Noemi Fusaki, Michael S. Sabel, Alfred E. Chang, Fumito Ito

“…TIL-derived iPSCs display embryonic stem cell-like morphology, have normal karyotype, express stem cell-specific surface antigens and pluripotency-associated transcription factors, and have the capacity to differentiate in vitro and in vivo. …”
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An advanced 3D lymphatic system for assaying human cutaneous lymphangiogenesis in a microfluidic platform by Minseop Kim, Sieun Choi, Dong-Hee Choi, Jinchul Ahn, Dain Lee, Euijeong Song, Hyun Soo Kim, Mijin Kim, Sowoong Choi, Soojung Oh, Minsuh Kim, Seok Chung, Phil June Park

“…The lymphatic system plays important roles in removing cell waste, residual proteins, various antigens, and immune cells from tissues to maintain homeostasis and activate the immune system through the drainage of interstitial fluid1,2. …”
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Spectral Imaging of Multi-Color Chromogenic Dyes in Pathological Specimens by Merryn V. E. Macville, Jeroen A. W. M. Van der Laak, Ernst J. M. Speel, Nir Katzir, Yuval Garini, Dirk Soenksen, George McNamara, Peter C. M. de Wilde, Antonius G. J. M. Hanselaar, Anton H.N. Hopman, Thomas Ried

“…A pixel‐by‐pixel spectrum‐based color classification is presented of single‐, double‐, and triple‐color in situ hybridization for centromeric probes in T24 bladder cancer cells, and immunocytochemical staining of nuclear antigens Ki‐67 and TP53 in paraffin‐embedded cervical brush material (AgarCyto). …”
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Lipoprotein Signal Peptide as Adjuvants: Leveraging Lipobox-Driven TLR2 Activation in Modern Vaccine Design by Muhammad Umar, Haroon Afzal, Asad Murtaza, Li-Ting Cheng

“…The lipidation process is essential for TLR2 recognition and the activation of robust immune responses, positioning lipidated bacterial proteins as potent immunomodulators and adjuvants for vaccines against bacterial-, viral-, and cancer-related antigens. The structural diversity and cleavage pathways of bacterial SPs are critical in determining lipidation efficiency and protein localization, influencing their immunogenic potential. …”
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HIV Progression Perturbs the Balance of the Cell-Mediated and Anti-Inflammatory Adaptive and Innate Mycobacterial Immune Response by Andrew R. DiNardo, Anna M. Mandalakas, Gugu Maphalala, Godwin Mtetwa, Temhlanga Mndzebele, Piluca Ustero, Makhosazana Hlatshwayo, Emily M. Mace, Jordan S. Orange, George Makedonas

“…Since T-lymphocyte production of interferon-gamma (IFN-γ) in response to Mycobacterium tuberculosis (Mtb) antigens fails to differentiate disease from latent infection, we applied a comprehensive profiling methodology to define immune biomarkers that reliably predict a patient’s TB risk. …”
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Multiplex Evaluation of Influenza Neutralizing Antibodies with Potential Applicability to In-Field Serological Studies by Eleonora Molesti, Edward Wright, Calogero Terregino, Rafat Rahman, Giovanni Cattoli, Nigel J. Temperton

“…This assay permits the measurement of neutralizing antibody responses against two antigenically distinct HAs in the same serum/plasma sample thus increasing the amount and quality of serological data that can be acquired from valuable sera. …”
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Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells by Margaret R. Dunne, Bozgana A. Mangan, Laura Madrigal-Estebas, Derek G. Doherty

“…Human Vγ9Vδ2 T cells recognise pyrophosphate-based antigens (phosphoantigens) and have multiple functions in innate and adaptive immunity, including a unique ability to activate other cells of the immune system. …”
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Therapeutic proteins immunogenicity: a peptide point of view by Feliciana Real-Fernandez, Fosca Errante, Andrea Di Santo, Anna Maria Papini, Paolo Rovero

“…This review includes studies describing antigenic peptides that enable enhanced ADA detection directly in patients’ sera, as well as the proof of concept that asses the use of peptides instead of proteins, to facilitate the identification of neutralizing ADA.…”
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Laparoscopic Splenectomy to Salvage Renal Transplants from Severe Acute Antibody-Mediated Rejection by Michael Latzko, Sakshi Jasra, Sana Akbar, Harry Sun, Sadanand Palekar

“…The patient had numerous class 1 unacceptable antigens. She was scheduled to undergo an incompatible 1-1-1 mismatch living related donor kidney transplant. …”
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Protective Efficacy of the Calicivirus Valency of the Leucofeligen Vaccine against a Virulent Heterologous Challenge in Kittens by Cynthia Lesbros, Virginie Martin, Wojciech Najbar, Annaele Sanquer, David Mcgahie, Hyone-Myong Eun, Sylvie Gueguen

“…Feline calicivirus (FCV) is a common feline pathogen with a potential for antigenic diversity. This study aimed to evaluate and characterize the protective efficacy of the FCV-F9 valency of a tetravalent vaccine, Leucofeligen, against challenge with an unrelated strain. …”
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HLA-G Expression Is an Independent Predictor for Improved Survival in High Grade Ovarian Carcinomas by M. J. Rutten, F. Dijk, C. D. Savci-Heijink, M. R. Buist, G. G. Kenter, M. J. van de Vijver, E. S. Jordanova

“…Aberrant expression of human leukocyte antigens (HLA) class I has prognostic importance in various cancers. …”
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Engineering hydrophobicity and manufacturability for optimized biparatopic antibody–drug conjugates targeting c-MET by Andreas Evers, Simon Krah, Deniz Demir, Ramona Gaa, Desislava Elter, Christian Schroeter, Stefan Zielonka, Nicolas Rasche, Julia Dotterweich, Christine Knuehl, Achim Doerner

“…The methodologies of in silico hydrophobicity identification and sequence optimization could serve as a blueprint for rapid development of optimal biparatopic ADCs targeting further tumor-associated antigens in the future.…”
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Effects of Severe Hypoxia on Bone Marrow Mesenchymal Stem Cells Differentiation Potential by Claudia Cicione, Emma Muiños-López, Tamara Hermida-Gómez, Isaac Fuentes-Boquete, Silvia Díaz-Prado, Francisco J. Blanco

“…BM-MSCs from the donors displayed only few phenotypical differences in surface antigens expressions. Analyzing marker genes expression levels of the treated cells compared to their control point for each lineage showed a good differentiation in normoxic conditions and the absence of this differentiation capacity in severe hypoxic cultures. …”
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Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia by Hui Liu, Rong Fu, Yihao Wang, Hong Liu, Lijuan Li, Honglei Wang, Jin Chen, Hong Yu, Zonghong Shao

“…Further studies are needed to confirm the antigenicity of these autoantigens.…”
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A novel therapeutic multiepitope vaccine based on oncoprotein E6 and E7 of HPV 16 and 18: An in silico approach by Sari Eka Pratiwi, Ysrafil Ysrafil, Mardhia Mardhia, Mahyarudin Mahyarudin, Muhammad Inam Ilmiawan, Heru Fajar Trianto, Delima Fajar Liana, Yuri Amia

“…Results: The constructed vaccine exhibited antigenic, immunogenic, nonallergenic, nontoxic, stable, and soluble characteristics. …”
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Photodynamic therapy for the precise treatment of localized prostate cancer by Youcheng Xu, Qinyuan Tan, Chong Sun, Yuefeng Jia, Shengxian Li, Xuecheng Yang

“…Over the past 20 years, early diagnosis of prostate cancer has become increasingly prevalent due to the promotion of prostate-specific antigens, and its treatment has become a focal point. …”
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Antarctic krill antioxidant peptides show inferior IgE-binding ability and RBL-2H3 cell degranulation by Jie Ding, Chunyan Zhu, Pengfei Jiang, Libo Qi, Na Sun, Songyi Lin

“…Enzymatic hydrolysis, isolation, and purification might make a great deal of difference in antioxidant activity and antigenicity of peptide components. This study aimed to isolate and purify antioxidant peptide components from Antarctic krill and evaluate their allergenicity of them. …”
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