Seroprevalence of brucellosis in camels and humans in the Al-Qassim region of Saudi Arabia and its implications for public health by Abdulaziz M. Almuzaini, Abdullah S. M. Aljohani, Ahmed I. Alajaji, Ayman Elbehiry, Adil Abalkhail, Abdulrahman Almujaidel, Sahar N. Aljarallah, Hazem R. Sherif, Eman Marzouk, Abdelmaged A. Draz

“…The Rose Bengal Test (RBT), Antigen Rapid Brucella Antibody Test (ARBT), indirect enzyme-linked immunosorbent assay (I-ELISA), and complement fixation test (CFT) were used to detect brucellosis in both camels and humans. …”
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Pancreatic cancer cell-intrinsic transglutaminase-2 promotes T cell suppression through microtubule-dependent secretion of immunosuppressive cytokines by Anton Wellstein, Thomas Seufferlein, Alexander Kleger, Tim Eiseler, Anton Lahusen, Nora Minhöfer, Kim-André Lohse, Christine Blechner, Jessica Lindenmayer, Sabine Windhorst, Yuan-Na Lin

“…Here, we identify a cancer cell-intrinsic mechanism that contributes to a T cell-resistant phenotype and describes potential combinatorial therapy.Methods We used an unbiased screening approach of T cell resistant and sensitive murine KPC (KrasLSL-G12D/+; Trp53fl/fl; Ptf1aCre/+) PDAC cells in a three-dimensional co-culture platform with syngeneic antigen-educated T cells to identify potential cell-intrinsic drivers of T cell suppression in PDAC. …”
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Autoantibodies in type 1 diabetes: Prevalence and clinical profiles by Raja Hazime, Saad Lamjadli, Morad Guennouni, Mounir Belkrachni, Fatima-Ezzohra Eddehbi, Ider Oujamaa, Lahcen Elmoumou, Aicha Bourrahouate, Imane Ait Sab, Hicham Baizri, Nawal El Ansari, Brahim Admou

“…The immunological investigation was based on the detection of T1D related a-Abs, including anti-insulin, anti-glutamic acid decarboxylase (GAD65), anti-insulinoma-associated antigen 2 (IA2), and anti-zinc transporter 8 (ZnT8) specificities. …”
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The Effect of Diabetes-Associated Autoantigens on Cell Processes in Human PBMCs and Their Relevance to Autoimmune Diabetes Development by Jana Vcelakova, Radek Blatny, Zbynek Halbhuber, Michal Kolar, Ales Neuwirth, Lenka Petruzelkova, Tereza Ulmannova, Stanislava Kolouskova, Zdenek Sumnik, Pavlina Pithova, Maria Krivjanska, Dominik Filipp, Katerina Stechova

“…Important pathways that were identified using data from the T1D versus controls comparison were pathways involving antigen presentation by MHCII, the activation of Th17 and Th22 responses, and cytoskeleton rearrangement-related processes. …”
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Key considerations based on pharmacokinetic/pharmacodynamic in the design of antibody-drug conjugates by Yangyang Gao, Yuwei Xia, Yixin Chen, Shiqi Zhou, Yingying Fang, Jieru Yu, Leyin Zhang, Leyin Zhang, Leitao Sun, Leitao Sun, Leitao Sun

“…Through the selection of target antigen, the optimization of the linker, the optimization of novel small molecule toxins as payload, the optimization of ADC, overcoming the multi-drug resistance of ADC, improving the ADC tumor penetration of ADC, surface modification of ADC and surface bystander effect of ADC provide a more comprehensive and accurate framework for designing new ADCs.ResultsWe’ve expounded comprehensively on applying pharmacokinetics or pharmacodynamics while designing ADC to obtain higher efficacy and fewer side effects. …”
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Nationwide representative serosurvey of third-grade school children to evaluate the hepatitis B vaccination impact in Kyrgyzstan, 2022 by Michael Brandl, Gulnara Zhumagulova, Gulbara Ishenapysova, Zuridin Nurmatov, Tatiana Enverovna Kuchuk, Nurzhan Zamirbekova, Gulsunai Sattarova, Saikal Temirbekova, Zhanara Bekenova, Martyna Gassowski, Liudmila Mosina, Antons Mozalevskis, Sandra Dudareva, Siddhartha Sankar Datta

“…In 2016, aligned with the goal of controlling hepatitis B in the WHO European Region, a regional target of 0.5% was set for seroprevalence of hepatitis B surface antigen (HBsAg) among targeted birth cohorts. We conducted a representative nationwide serosurvey to assess the HBsAg prevalence among third-grade school children in Kyrgyzstan in 2022. …”
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Association between clinical-biological characteristics of Klebsiella pneumoniae and 28-day mortality in patients with bloodstream infection by Xiaofeng Yu, Yi Zhao, Xiao Sun, Jiahui Luan, Haiyan Wang, Tianyu Sun, Tongtong Lin, Xia Zhou, Wei Yang, Ziguang Deng, Bo Liu, Hongyun Cao

“…Conclusion The carriage of virulence genes rfbA/B/D, which is responsible for the synthesis of O-antigen, was associated with poor prognosis of KP BSI. …”
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Characteristics of TSPO expression in marmoset EAE by Irene Falk, Dragan Maric, Emily Leibovitch, Pascal Sati, Jennifer Lefeuvre, Nicholas J. Luciano, Joseph Guy, Seung-Kwon Ha, David R. Owen, Franklin Aigbirhio, Paul M. Matthews, Daniel S. Reich, Steven Jacobson

“…In Iba1 + cells in both control and acute EAE tissue, we find that TSPO is co-expressed with markers of antigen presentation (CD74), early activation (MRP14), phagocytosis (CD163) and anti-inflammatory phenotype (Arg1); a high level of TSPO expression is not restricted to a particular microglial phenotype. …”
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Comparison of clinical and virological features in pediatric and adult dengue cases at Insein General Hospital during Myanmar’s 2022 dengue season by Htin Lin, Mya Myat Ngwe Tun, Yin Mon Wint Zin, Khin Theingi Myint, Win Kay Khine, Khine Mya Nwe, Aye Aye Thant, Thin Thin Shwe, Win Mar, Khin Sandar Aye, Khaing Moe Aung, Yuki Takamatsu, Wah Wah Aung, Yi Yi Kyaw, Takeshi Urano, Kouichi Morita, Hlaing Myat Thu

“…Results The dengue non-structural protein-1 antigen (NS1Ag) positivity was 37% in children and 32% in adults. …”
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Distribution of Major HLA-A, -B, -DR, and -DQ Loci Potentially Associated with Multiple Sclerosis in a Healthy Population from Southern Morocco by Abir Fguirouche, Fatimazahra Ouahmani, Ikram Brahim, Raja Hazime, Nissrine Louhab, Najib Kissani, Mohamed Chraa, Brahim Admou

“…<b>Background:</b> Many factors contribute to the development and the progression of Multiple Sclerosis (MS), including Human Leukocyte Antigen (HLA) molecules. Some of them are considered as predisposing, like DRB1*15, DRB1*13, DRB1*03, DRB1*04, DQB1*06, DQB1*02, while HLA A2, HLA B44, DRB1*11, and DRB1*12 are rather considered as protective. …”
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FORCE platform overcomes barriers of oligonucleotide delivery to muscle and corrects myotonic dystrophy features in preclinical models by Timothy Weeden, Tyler Picariello, Brendan Quinn, Sean Spring, Pei-Yi Shen, Qifeng Qiu, Benjamin F. Vieira, Lydia Schlaefke, Ryan J. Russo, Ya-An Chang, Jin Cui, Monica Yao, Aiyun Wen, Nelson Hsia, Tama Evron, Katy Ovington, Pei-Ni Tsai, Nicholas Yoder, Bo Lan, Reshmii Venkatesan, John Hall, Cody A. Desjardins, Mo Qatanani, Scott Hilderbrand, John Najim, Zhenzhi Tang, Matthew K. Tanner, Romesh Subramanian, Charles A. Thornton, Oxana Ibraghimov-Beskrovnaya, Stefano Zanotti

“…The platform consists of an antigen-binding fragment, highly specific for the human transferrin receptor 1 (TfR1), conjugated to an oligonucleotide via a cleavable valine-citrulline linker. …”
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Immune Dysfunction in Rett Syndrome Patients Revealed by High Levels of Serum Anti-N(Glc) IgM Antibody Fraction by Anna Maria Papini, Francesca Nuti, Feliciana Real-Fernandez, Giada Rossi, Caterina Tiberi, Giuseppina Sabatino, Shashank Pandey, Silvia Leoncini, Cinzia Signorini, Alessandra Pecorelli, Roberto Guerranti, Solange Lavielle, Lucia Ciccoli, Paolo Rovero, Claudio De Felice, Joussef Hayek

“…To determine immunoglobulins we used both a conventional agglutination assay and a novel ELISA based on antibody recognition by a surrogate antigen probe, CSF114(Glc), a synthetic N-glucosylated peptide. …”
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Altered immune surveillance of B and T cells in patients with persistent residual lung abnormalities 12 months after severe COVID-19 by Julio Flores-Gonzalez, Ivette Buendia-Roldan, Fernanda Téllez-Quijada, Carlos Peña-Bates, Lucero A. Ramón-Luing, Armando Castorena-Maldonado, Ramcés Falfán-Valencia, Gloria Pérez-Rubio, Moisés Selman, Leslie Chavez-Galan, Leslie Chávez-Galán

“…Cell profiling analysis was done using flow cytometry at 24 h of not antigen-specific in vitro stimulation. Plasma or supernatant levels of IFN-g, IL-4, IL-10, IgM, and IgG were assessed, and 10 patients (5 FRG, 5 PLAG) were randomly selected for detailed immune cell phenotyping and functional analysis of peripheral blood mononuclear cells using flow cytometry. …”
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Adjuvant-free, self−assembling ferritin nanoparticle vaccine coupled with influenza virus hemagglutinin protein carrying M1 and PADRE epitopes elicits cross-protective immune respo... by Yongqiang Zhao, Shuangshuang Guo, Jia Liu, Yating Wang, Bo Wang, Chun Peng, Enqi Du, Enqi Du, Enqi Du

“…This nanoparticle vaccine displayed multi-antigen targets on the surface of Helicobacter pylori ferritin, which consists of the ectodomain of hemagglutinin of the H3N2 virus and three tandem highly conserved influenza M1 epitopes fused with the universal helper T-cell epitope PADRE, named HMP-NP. …”
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Safety profile of sacituzumab govitecan in patients with breast cancer: A systematic review and meta-analysis by Maria Inez Dacoregio, Isabella Michelon, Caio Ernesto do Rego Castro, Francisco Cezar Aquino de Moraes, Guilherme Rossato de Almeida, Lis Victória Ravani, Maysa Vilbert, Ricardo Lima Barros Costa

“…Background: Sacituzumab Govitecan (SG), a first-in-class anti-trophoblast cell surface antigen-2-directed antibody-drug conjugate (ADC), has shown clinically meaningful improvement in outcomes of patients with breast cancer (BC). …”
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Construction and characterization of chimeric FcγR T cells for universal T cell therapy by Juanjuan Zhao, Manling Chen, Xudong Li, Zhaoqi Chen, Wei Li, Rongqun Guo, Min Wang, Zhongxing Jiang, Yongping Song, Jianxiang Wang, Delong Liu

“…Abstract Background Several approaches are being explored for engineering off-the-shelf chimeric antigen receptor (CAR) T cells. In this study, we engineered chimeric Fcγ receptor (FcγR) T cells and tested their potential as a versatile platform for universal T cell therapy. …”
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Protocol for a randomised phase 3 trial evaluating the role of Finasteride in Active Surveillance for men with low and intermediate-risk prostate cancer: the FINESSE Study by Samuel Smith, Peter Sasieni, William Cross, Richard Bryant, Steven Kennish, James Catto, Rachel Hubbard, Simon Faulkner, Rohit Chahal, Mohantha Dooldeniya, Marcus Cumberbatch, Bernard North, Alastair D Lamb, Roseann Kealy, Selina Bhattrai

“…AS includes regular prostate-specific antigen testing, MRI scans and the offer of repeat biopsy (at 3 years, or if imaging suggests progression). …”
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Differential impact of TIM-3 ligands on NK cell function by Xin Zhang, Lazar Vujanovic, Riyue Bao, Robert L Ferris, Tullia C Bruno, Aditi Kulkarni, Hridesh Banerjee, Housaiyin Li, Juncheng Wang, Lawrence P Kane, Pragati Upadhyay, Jennifer L Anderson, Onyedikachi Victor Onyekachi, Lidia M R B Arantes

“…An effective TIM-3-targeting therapy should account for multiple biological factors, including the disease setting, the specific cell types involved and their varying sensitivities to the four putative TIM-3 ligands (galectin-9, phosphatidylserine, high mobility group protein B1 and carcinoembryonic antigen cell adhesion molecule 1), each of which engages a unique binding site on the receptor’s variable immunoglobulin domain. …”
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Impact of the COVID-19 Pandemic on Epidemiological Trends in Pediatric Cervical Abscess-Forming Infections by Shuhei Takahashi, Ai Kishino, Kentaro Miyai, Shigeru Takishima, Tae Omori, Hidehiro Furuno, Ryosei Iemura, Makoto Ono, Keisuke Ogasawara, Akito Sutani, Masayuki Nagasawa

“…No significant changes were found in the pharyngeal streptococcal antigen positivity rate, rate of oral antibiotic use before hospitalization, length of hospital stay, or duration of antibiotic administration before and after the COVID-19 pandemic. …”
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Investigation of 23 Bile Acids in Liver Bile in Benign and Malignant Biliary Stenosis: A Pilot Study by Stanislav Rejchrt, Milos Hroch, Rudolf Repak, Tomas Fejfar, Tomas Douda, Darina Kohoutova, Eva Peterova, Jan Bures

“…Laboratory markers (serum bilirubin>75 μmol/L, carbohydrate antigen 19-9>400 U/mL) and the length of stenosis (>15 mm) can be helpful but are not specific enough. …”
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