Diagnostic Performance of Three ELISAs for Detection of Antibodies against SARS-CoV-2 in Human Samples by Cássio Meira, Dahara Silva, Ivanilson Santos, Breno Barreto, Vinícius Rocha, Emanuelle Santos, Bruna dos Reis, Afrânio Evangelista, Ricardo Ribeiro dos Santos, Bruna Machado, Guilherme Ribeiro, Roberto Badaró, Milena Soares

“…Based on this, we evaluated three enzyme-linked immunoassays (ELISAs) using different antigens from SARS-CoV-2 in a cohort of 161 patients. …”
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Persistence of the Immune Response to an Intramuscular Bivalent (GI.1/GII.4) Norovirus Vaccine in Adults by Geert Leroux-Roels, Robert L. Atmar, Jakob P. Cramer, Ian Escudero, Astrid Borkowski

“…Methods: In this study, 428 adult recipients of various compositions of the norovirus vaccine candidate, HIL-214, were followed for 5 years, to assess immune responses to its virus-like particle antigens, GI.1 and GII.4c. Serum antibodies and peripheral-blood antibody-secreting cells (ASCs) were measured. …”
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Cell-associated flagella enhance the protection conferred by mucosally-administered attenuated Salmonella Paratyphi A vaccines. by Orit Gat, James E Galen, Sharon Tennant, Raphael Simon, William C Blackwelder, David J Silverman, Marcela F Pasetti, Myron M Levine

“…Paratyphi A vaccine, we addressed the role of flagella as a potential protective antigen by comparing cell-associated flagella with exported flagellin subunits expressed by attenuated strains.…”
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A new immunofluorescence determination of Parkinson's disease biomarkers using silver nanoparticles by Chao Qin, Jun Xia, Yong Wen, Jun Wang, Chen Zhong

“…The system's workings are based on antigen-antibody complexes. Identified as Ab1-MBs@α-syn@Ab2-Ag NPs, the immuno-complexes encapsulate α-synuclein between anti-α-synuclein antibodies (Ab1) fixed on amino-MBs and Ag Nanoparticles functionalized with matching Ab2. α-synuclein detection was accomplished at a limit of less than 8 pg/mL through optimization of pH, reaction duration, and antibody concentration. …”
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A Case of Mild Encephalopathy with a Reversible Splenial Lesion Associated with G5P[6]Rotavirus Infection by Tsuyoshi Matsuoka, Toshifumi Yodoshi, Misaki Sugai, Masato Hiyane, Takashi Matsuoka, Hideki Akeda, Masaharu Ohfu, Satoshi Komoto, Koki Taniguchi

“…On day 8 of admission, head MRI revealed that the splenial lesion had disappeared. RV antigen-positive stools suggested that RV had caused MERS. …”
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Nomogram for prognosis prediction in metastatic pancreatic cancer patients undergoing intra-arterial infusion chemotherapy: incorporating immune-inflammation scores and coagulation... by Yifan Yang, Shaoqi Zong, Yongqiang Hua

“…Through Cox regression analysis, a nomogram was ultimately constructed incorporating NLR, PLR, PT, alkaline phosphatase (ALP), carbohydrate antigen 125 (CA125), age, and ablation. The model demonstrated good discriminative ability, with a C-index of 0.722, and the AUC values at 6- and 12-month OS predictions were 0.828 and 0.851 in the training cohort, while in the validation cohort, the corresponding AUC values were 0.754 and 0.791, respectively. …”
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Regulation of Murine Ovarian Epithelial Carcinoma by Vaccination against the Cytoplasmic Domain of Anti-Müllerian Hormone Receptor II by Cagri Sakalar, Suparna Mazumder, Justin M. Johnson, Cengiz Z. Altuntas, Ritika Jaini, Robert Aguilar, Sathyamangla V. Naga Prasad, Denise C. Connolly, Vincent K. Tuohy

“…We found that AMHR2-CD immunization of C57BL/6 females induced a prominent antigen-specific proinflammatory CD4+ T cell response that resulted in a mild transient autoimmune oophoritis that resolved rapidly with no detectable lingering adverse effects on ovarian function. …”
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Clinical Investigation of Benign Asbestos Pleural Effusion by Nobukazu Fujimoto, Kenichi Gemba, Keisuke Aoe, Katsuya Kato, Takako Yokoyama, Ikuji Usami, Kazuo Onishi, Keiichi Mizuhashi, Toshikazu Yusa, Takumi Kishimoto

“…Mean values of hyaluronic acid, adenosine deaminase, and carcinoembryonic antigen in the pleural fluid were 39,840 ng/mL, 23.9 IU/L, and 1.8 ng/mL, respectively. …”
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Predictors of HBsAg seroclearance in HBeAg-negative chronic hepatitis B patients treated with nucleotide analogs plus polyethylene glycol interferon by Yan Peng, Mingzhe Ma, Ting Liu, Wenmin He, Shutao Lin, Wa Zhong, Xiaohui Min

“…IntroductionThe minority of the chronic hepatitis B (CHB) patients received polyethylene glycol interferon (PEG-IFN) combined with nucleotide analogs (NAs) can obtain hepatitis B surface antigen (HBsAg) clearance.MethodsIn order to find out the advantaged population, we retrospectively collected 122 CHB patients treated with NAs alone or NAs plus PEG-IFN for 48 weeks, who were admitted to Sun Yat-sen Memorial Hospital from 2019 to 2024.ResultsWe found HBsAg clearance rate in NAs plus PEG-IFN group was 40.98%, which was significantly higher than that in the NAs group. …”
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GITR-GITRL System, A Novel Player in Shock and Inflammation by Ludovic Tibor Krausz, Rodolfo Bianchini, Simona Ronchetti, Katia Fettucciari, Giuseppe Nocentini, Carlo Riccardi

“…GITR is activated by its ligand, GITRL, mainly expressed on antigen presenting and endothelial cells. Recent evidence suggests that the GITR/GITRL system participates in the development of inflammatory responses, including shock, either due to early response of neutrophils and macrophages, or together with autoimmune/allergic pathogenesis. …”
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Evaluation of serum amylase and lipase in diagnosed Covid-19 survivors in a North Indian Hospital, Lucknow, India by Nabeela Usmani, Mukhtar Ahmad, Saba Khan, Mohammad Mustufa Khan, Haseeb Ahsan, Sadaf Parveen, Roshan Alam

“…Two groups of subjects (70 adults consisting of 35 Covid-19 survivors and 35 controls) aged between 35 to 70 years were selected for the study with mild to moderate Covid-19 diagnosed using RT-PCR/rapid antigen test and a control group of healthy individuals without history of Covid-19. …”
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Molecular Serotyping and Antibiotic Resistance Patterns of Escherichia coli Isolated in Hospital Catering Service in Morocco by Benjelloun Touimi Ghita, Laila Bennani, Sanae Berrada, Moussa Benboubker, Bahia Bennani

“…Molecular amplification shows that 14 E. coli isolates carried the flagellar antigen H7, while there are no isolates showing amplification for O157. …”
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Correlation of Clinical Severity and Laboratory Parameters with Various Serotypes in Dengue Virus: A Hospital-Based Study by Pooja Rao, Achappa Basavaprabhu, Suchitra Shenoy, Nikhil Victor Dsouza, Basavaiah Sridevi Hanaganahalli, Vaman Kulkarni

“…A prospective study was conducted among 106 patients admitted with acute febrile illness having positive NS1 antigen/IgM ELISA. Clinical data were extracted from medical records including demographics, presence of comorbid conditions, clinical presentation, laboratory investigations, and course including length of hospital stay and outcome. …”
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Glut3 overexpression improves environmental glucose uptake and antitumor efficacy of CAR-T cells in solid tumors by Hui Wang, Yi Zhang, Feng Li, Shasha Liu, Shumin Wang, Wenhao Hu, Yue Liang, Chunyi Shen, Yuyu Zhao

“…Furthermore, glucose restriction can contribute to the failure of chimeric antigen receptor T (CAR-T) cell therapy for solid tumors. …”
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CRISPR/Cas9-mediated genomic insertion of functional genes into Lactiplantibacillus plantarum WCFS1 by Kamilla Wiull, Lisa K. Haugen, Vincent G. H. Eijsink, Geir Mathiesen

“…Our system successfully knock-in four expression cassettes varying in length from ~800–1,300 bp with high efficiency and insert an expression cassette encoding a SARS-CoV-2 antigen receptor-binding domain (RBD) with an anchor mediating surface display, which has not been achieved previously using CRISPR/Cas9. …”
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Extracellular vesicle surface engineering with integrins (ITGAL & ITGB2) to specifically target ICAM-1-expressing endothelial cells by Markus Bergqvist, Kyong-Su Park, Nasibeh Karimi, Lijuan Yu, Cecilia Lässer, Jan Lötvall

“…To target these cells, we overexpress the ICAM-1 binding receptor “lymphocyte function-associated antigen-1” (LFA-1) in HEK293F cells, by sequential transfection of plasmids of the two LFA-1 subunits, ITGAL and ITGB2 (CD11a and CD18). …”
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Diabetes insipidus and Guillain-Barré-like syndrome following CAR-T cell therapy: a case report by Christian Koch, Patrick Roth, Juliane Fleischer, Todor Popov, Karl Frontzek, Bettina Schreiner, Markus G. Manz, Simone Unseld, Antonia M. S. Müller, Norman F. Russkamp

“…Background Immune effector cell-associated neurotoxicity syndrome (ICANS) is a common adverse event of CD19-directed chimeric antigen receptor (CAR) T cell therapy. Other neurological adverse events, however, have not methodically been described and studied. …”
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Imply on diagnosis and early prognosis of preoperative [68Ga]Ga-PSMA-11 PET/CT in patients with suspected brain tumours of glial origin by K. Pełka, K. Koczyk, L. Koperski, T. Dziedzic, A. Nowak, L. Królicki, P. Kunert, J. Kunikowska

“…Abstract PET/CT targeting prostate-specific membrane antigen (PSMA) is commonly used in patients with prostate cancer. …”
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Targeting mutant p53: Evaluation of novel anti-p53R175H monoclonal antibodies as diagnostic tools by Diana Spiegelberg, Le-Ann Hwang, Khian Hong Pua, Sashwini Chandra Kumar, Xin Yu Koh, Xiao Hui Koh, Ram Kumar Selvaraju, Kanaga Sabapathy, Marika Nestor, David Lane

“…In vitro, 125I-4H5 and 125I-7B9 demonstrated long shelf life and antigen-specific binding. Our in vivo study design allowed head-to-head comparison of the antibodies in a double tumor model using repeated SPECT/CT imaging, followed by biodistribution and autoradiography. …”
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Insulin autoimmune syndrome in a 3-year-old boy: the youngest to date in China and a narrow review by Ming Cheng, Yuan Ding, Dongmei Wang, Chunxiu Gong

“…High-resolution human leukocyte antigen (HLA) test showed a DRB1*08:03/*12:02 genotype. …”
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