A Spike-Based mRNA Vaccine Encapsulated in Phospholipid 1,2-Dioleoyl-sn-Glycero-3-PhosphoEthanolamine Containing Lipid Nanoparticles Induced Potent B- and T-Cell Responses Associat... by Afshana Quadiri, Swayam Prakash, Latifa Zayou, Nisha Rajeswari Dhanushkodi, Amruth Chilukuri, Gemma Ryan, Kelly Wang, Hawa Vahed, Aziz A. Chentoufi, Lbachir BenMohamed

“…While the LNPs protect mRNA from degradation and efficiently deliver the mRNA to antigen-presenting cells, the effect of lipid composition on the immunogenicity and protective efficacy of mRNA/LNP vaccines is not well characterized. …”
Get full text

Results from the Population-Based Gutenberg Health Study Revealing Four Altered Autoantibodies in Retinal Vein Occlusion Patients by Katharina Bell, Vanessa M. Beutgen, Stefan Nickels, Katrin Lorenz, Yvonne Scheller, Hisham Elbaz, Tunde Peto, Katharina A. Ponto, Andreas Schulz, Philipp S. Wild, Thomas Münzel, Karl J. Lackner, Irene Schmidtmann, Manfred Beutel, Norbert Pfeiffer, Franz H. Grus, Alexander K. Schuster

“…Of all RVO subjects and controls, serum autoantibody profiles were measured using in-house manufactured antigen-antibody microarrays. Results. Of the 59 RVO patients, 3 patients (5%) showed glaucomatous optic disc alterations at baseline, whereas no new glaucoma case was detected at 5-year follow-up. …”
Get full text

CT45A1‐mediated MLC2 (MYL9) phosphorylation promotes natural killer cell resistance and outer cell fate in a cell‐in‐cell structure, potentiating the progression of microsatellite... by Hao‐Wei Teng, Hsiang‐Yueh Huang, Chun‐Chi Lin, Yuh‐Ching Twu, Wen‐Hao Yang, Wen‐Chun Lin, Hsin‐Yi Lan, Yen‐Yu Lin, Wei‐Lun Hwang

“…We showed that cancer/testis antigen family 45 member A1 (CT45A1) expression was upregulated in MSI‐H CRC patients with poor survival outcomes. …”
Get full text

Biochemical failure-free survival of 18F-rhPSMA-7 and 18F-flotufolastat PET-guided salvage radiotherapy for patients with recurrent prostate cancer by Marco M. E. Vogel, Isabel Rauscher, Jürgen E. Gschwend, Türkay Hekimsoy, Nicola Gabler, Charlotte Olufs, Calogero D’Alessandria, Jan C. Peeken, Stephanie E. Combs, Matthias Eiber

“…Abstract Prostate-specific membrane antigen (PSMA)-targeted positron emission tomography (PET) has improved localization of prostate cancer (PC) lesions in biochemical recurrence (BCR) for salvage radiotherapy (SRT). …”
Get full text

High peripheral T cell diversity is associated with lower risk of toxicity and superior response to dual immune checkpoint inhibitor therapy in patients with metastatic NSCLC by Alexandre Reuben, Mehmet Altan, Latasha Little, Jianjun Zhang, Ajay Sheshadri, Hai T Tran, Mara B Antonoff, Stephen G Swisher, John V Heymach, Saumil Gandhi, Natalie Vokes, Ziyi Li, Ruoxing Li, Runzhe Chen, Joshua Baguley, Jefferson Sinson, Greg Lizee

“…For analysis of T-cell repertoire, we performed immunoSEQ to assess the complementary determining region 3β region of the TCR involved in antigen binding.Results A total of 250 samples from 119 patients were analyzed. …”
Get full text

Diez años del “Programa de evaluación de desempeño de laboratorios de inmunogenética” y su impacto en la “Red de donación y trasplantes” by yazmin rocio arias-murillo, María Angélica Salinas-Nova, Yesith Guillermo Toloza-Pérez, Miguel Ángel Castro-Jiménez

“…En las pruebas de HLA (2014), PRA cualitativo (2017 y 2019), pruebas cruzadas (2019) y single antigen (2017 y 2019), el porcentaje de laboratorios con desempeño catalogado como «bueno» fue menor que el esperado. …”
Get full text

Single dose of recombinant baculovirus vaccine expressing sigma B and sigma C genes provides good protection against novel duck reovirus challenge in ducks by Yufei Wang, Siyi Xu, Xia Chen, Yunlong Dou, Xingzhu Yang, Zenglei Hu, Shuang Wu, Xiaoquan Wang, Jiao Hu, Xiufan Liu

“…The double antibody-sandwich ELISA was used for quantitative of σB or σC protein in the inactivated crude antigen. Protective efficacy results revealed that, compared with the whole virus inactivated vaccine, a single dose of 160 ng σB or σC protein showed advantages in inducing serum antibodies, elevating weight, alleviating liver and spleen injury, restraining viral shedding and viral replication in ducklings. …”
Get full text

Dysregulated Circulating Dendritic Cell Function in Ulcerative Colitis Is Partially Restored by Probiotic Strain Lactobacillus casei Shirota by Elizabeth R. Mann, Jialu You, Verena Horneffer-van der Sluis, David Bernardo, Hafid Omar Al-Hassi, Jon Landy, Simon T. Peake, Linda V. Thomas, Cheng T. Tee, Gui Han Lee, Ailsa L. Hart, Parveen Yaqoob, Stella C. Knight

Get full text

Intracardiac Fibrinolysis and Endothelium Activation Related to Atrial Fibrillation Ablation with Different Techniques by Orsolya Hajas, Zsuzsa Bagoly, Noémi K Tóth, Réka Urbancsek, Alexandra Kiss, Kitti B Kovács, Ferenc Sarkady, Attila Nagy, Anna V. Oláh, László Nagy, Marcell Clemens, László Csiba, Zoltán Csanádi

“…A similar trend was observed with IRF (p=0.0676). Both VWF antigen levels and FVIII activity increased after PVI with all the 3 techniques. …”
Get full text

The Role of EGFR/PI3K/Akt/cyclinD1 Signaling Pathway in Acquired Middle Ear Cholesteatoma by Wei Liu, Hongmiao Ren, Jihao Ren, Tuanfang Yin, Bing Hu, Shumin Xie, Yinghuan Dai, Weijing Wu, Zian Xiao, Xinming Yang, Dinghua Xie

“…We examined the expressions of phosphorylated EGF receptor (p-EGFR), phosphorylated Akt (p-Akt), cyclinD1, and proliferating cell nuclear antigen (PCNA) in 40 cholesteatoma samples and 20 samples of normal external auditory canal (EAC) epithelium by immunohistochemical method. …”
Get full text

B7-H3 CAR-T cell therapy combined with irradiation is effective in targeting bulk and radiation-resistant chordoma cancer cells by Kun Wang, Gianpietro Dotti, Soldano Ferrone, Yufeng Wang, Yin P Hung, Tao Ma, Giulia Cattaneo, Xinhui Wang, David O Osei-Hwedieh, Tara A Walhart, Joseph H Schwab

“…Additionally, increased expression of B7-H3 antigens on CCSCs and bulk tumor cells is associated with enhanced CAR-T cell killing in vitro and in vivo xenograft mouse models. …”
Get full text

Comparative study of mouse models of atopic dermatitis by Siqi Ye, Lian Zhu, Tao Ruan, Jinjing Jia, Xiumei Mo, Fenggen Yan, Junfeng Liu, Yu Zhang, Dacan Chen

“…The most common irritant was 2,4-dinitrochlorobenzene (DNCB), followed by Dermatophagoides farinae mite antigen extract (DfE). The modeling period was generally 21–30 days. …”
Get full text

Peptide-based vaccine targeting IL17A attenuates experimental spondyloarthritis in HLA-B27 transgenic rats by Tetsuya Tomita, Hiromi Rakugi, Hiroki Hayashi, Ryuichi Morishita, Jiao Sun, Yuka Yanagida, Shota Yoshida, Satoshi Baba, Akiko Tenma, Masayoshi Toyoura, Sotaro Kawabata, Takako Ehara, Ryoko Asaki, Makoto Sakaguchi, Hideki Tomioka, Munehisa Shimamura, Hironori Nakagami

“…Objectives Spondyloarthritis (SpA) is known as series of immune-mediated inflammatory disease of the axial and peripheral joints. Human leucocyte antigen (HLA)-B27 is a genetic risk factor for SpA. …”
Get full text

The Role of Hibiscus sabdariffa L. (Roselle) in Maintenance of Ex Vivo Murine Bone Marrow-Derived Hematopoietic Stem Cells by Zariyantey Abdul Hamid, Winnie Hii Lin Lin, Basma Jibril Abdalla, Ong Bee Yuen, Elda Surhaida Latif, Jamaludin Mohamed, Nor Fadilah Rajab, Chow Paik Wah, Muhd Khairul Akmal Wak Harto, Siti Balkis Budin

“…Roselle enhanced the survival (P<0.05) of BMCs at 500 and 1000 ng/mL, increased survival of Sca-1+ cells (HSCs) at 500 ng/mL, and maintained HSCs phenotype as shown from nonremarkable changes of surface marker antigen (Sca-1) expression in all experimental groups. …”
Get full text

CD4 T cell depletion increases memory differentiation of endogenous and CAR T cells and enhances the efficacy of Super2 and IL-33-armored CAR T cells against solid tumors by Chrystal Paulos, Mary Jo Turk, Megen C Wittling, Yina H Huang, Asmaa O Mohamed, David Tyler Boone, Shannon L Ferry, Melanie C Peck, Alicia M Santos, Haille E Soderholm

“…Background Responsiveness to chimeric antigen receptor (CAR) T cell therapy correlates with CAR T cell expansion and persistence in vivo. …”
Get full text

Combining a WT1 Vaccine (Galinpepimut-S) With Checkpoint Inhibition (Nivolumab) in Patients With WT1–Expressing Diffuse Pleural Mesothelioma: A Phase 1 Study by Prashasti Agrawal, MD, Michael Offin, MD, Victoria Lai, MD, Michelle S. Ginsberg, MD, Prasad S. Adusumilli, MD, FACS, Valerie W. Rusch, MD, Jennifer L. Sauter, MD, Teresa Ho, BS, Phillip Wong, PhD, Marjorie G. Zauderer, MD

“…Galinpepimut-S (GPS), a tetravalent, non–human leukocyte antigen–restricted, heteroclitic WT1–specific peptide vaccine was safe and effective in early phase clinical trials and upregulates T-cell suppressive programmed death-ligand 1 in the tumor microenvironment of other malignancies. …”
Get full text

Vector‐Free Deep Tissue Targeting of DNA/RNA Therapeutics via Single Capacitive Discharge Conductivity‐Clamped Gene Electrotransfer by Jeremy L. Pinyon, Georg vonJonquieres, Stephen L. Mow, Amr Al Abed, Keng‐Yin Lai, Mathumathi Manoharan, Edward N. Crawford, Stanley H. Xue, Sarah Smith‐Moore, Lisa J. Caproni, Sarah Milsom, Matthias Klugmann, Nigel H. Lovell, Gary D. Housley

“…Delivery of DNAs encoding secreted alkaline phosphatase and Cal/09 influenza virus hemagglutinin antigen generate high systemic circulating recombinant protein levels and antibody titres. …”
Get full text

Spontaneous high clonal expansion of Wilms’ tumor gene 1-specific cytotoxic T-lymphocytes in patients with Wilms’ tumor gene 1-expressing solid tumor by Soyoko Morimoto, Yukie Tanaka, Jun Nakata, Fumihiro Fujiki, Kana Hasegawa, Hiroko Nakajima, Sumiyuki Nishida, Akihiro Tsuboi, Naoki Hosen, Naoki Kagawa, Motohiko Maruno, Akira Myoui, Takayuki Enomoto, Shuichi Izumoto, Mitsugu Sekimoto, Naoya Hashimoto, Toshiki Yoshimine, Atsushi Kumanogoh, Yusuke Oji, Yoshihiro Oka, Haruo Sugiyama

“…The frequencies of WT1126-CTLs were higher in human leukocyte antigen (HLA)-A*02:01+ PTs than in HLA-A*02:01+ HVs, although the difference was not statistically significant. …”
Get full text

Unveiling causal immune cell–gene associations in multiple myeloma: insights from systematic reviews and Mendelian randomization analyses by Hui Zhang, Hui Zhang, Ling Zhang, Jing-Xuan Lian, Zhi-Fu Kou, Yu Zhu, Yu Zhu, Li-Tian Ma, Li-Tian Ma, Jin Zheng, Jin Zheng, Can-Jun Zhao, Can-Jun Zhao

“…BackgroundThe efficacy of novel chimeric antigen receptor T-cell (CAR-T) therapy is inconsistent, likely due to an incomplete understanding of the tumor microenvironment (TME). …”
Get full text

Clinical Characteristics and Treatment Options of Peripheral Spondyloarthritis by ZENG Lulu, JI Xiaojian, HU Lidong, HU Jiawen, ZHANG Yinan, ZHANG Jiaxin, LIU Xingkang, YANG Shiwei, HUANG Feng

“…Compared to axSpA patients, pSpA patients had fewer male patients(62.5% vs. 79.7%, P < 0.001), later onset age(33.8 years vs. 22.0 years, P < 0.001), shorter diagnostic delays(6.0 months vs. 14.2 months, P=0.004), more associated peripheral arthritis(71.7% vs. 9.3%, P < 0.001) and dactylitis(6.5% vs. 0.3%, P < 0.001), more cases of psoriasis(52.7% vs. 1.1%, P < 0.001) and a more common family history of psoriasis(11.4% vs. 3.4%, P < 0.001). pSpA patients had higher levels of inflammatory markers but a lower positive rate of human leukocyte antigen(HLA)-B27(43.5% vs. 87.4%, P < 0.001). A positive HLA-B27 was associated with an earlier onset age, fewer cases of psoriasis, and a family history of ankylosing spondylitis. pSpA patients had a higher proportion of using conventional synthetic disease-modifying antirheumatic drugs(csDMARDs), biologic disease-modifying antirheumatic drugs(bDMARDs), and oral glucocorticoids, and they also more frequently used a combination of bDMARDs and csDMARDs(19.0% vs. 12.2%, P=0.009) or multiple csDMARDs(65.8% vs. 12.5%, P < 0.001). …”
Get full text