NIAID Workshop Report: Systematic Approaches for ESKAPE Bacteria Antigen Discovery by Inka Sastalla, Keehwan Kwon, Clayton Huntley, Kimberly Taylor, Liliana Brown, Tamika Samuel, Lanling Zou

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Equivariant Hopf Bifurcation in a Time-Delayed Ring of Antigenic Variants by Israel Ncube

“…We consider an intrahost malaria model allowing for antigenic variation within a single species. The host’s immune response is compartmentalised into reactions to major and minor epitopes. …”
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Exposure to Environmental Antigens Induces the Development of Germinal Centers in Premature Neonates by N. Wittenbrink, M. Zemlin, K. Bauer, C. Berek

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Borrelia burgdorferi radiosensitivity and Mn antioxidant content: antigenic preservation and pathobiology by Andrés F. Londoño, Ajay Sharma, Jared Sealy, Vipin S. Rana, Shelby D. Foor, Vera Y. Matrosova, Elena K. Gaidamakova, Robert P. Volpe, Michael J. Daly, Brian M. Hoffman, Utpal Pal, J. Stephen Dumler

“…These observations also provide an opportunity for innovative vaccine development strategies employing ionizing radiation to disrupt the B. burgdorferi genome, while maintaining antigenic potency. These fresh insights extend our understanding of the unique biology of B. burgdorferi and open new avenues for considering novel whole-cell Lyme disease vaccines using MDP and irradiation-based inactivation.IMPORTANCEThe study highlights that electron paramagnetic resonance (EPR) spectroscopy is sufficiently sensitive to detect small differences in radiation resistance among Borrelia burgdorferi strains based on their population of Mn2+-metabolite complexes (H-Mn). …”
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Leveraging mRNA technology for antigen based immuno-oncology therapies by Wei Zheng, Michele Ceccarelli, Charalampos S Floudas, Siranush Sarkizova

“…The application of messenger RNA (mRNA) technology in antigen-based immuno-oncology therapies represents a significant advancement in cancer treatment. …”
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Toward enhancement of antibody thermostability and affinity by computational design in the absence of antigen by Mark Hutchinson, Jeffrey A. Ruffolo, Nantaporn Haskins, Michael Iannotti, Giuliana Vozza, Tony Pham, Nurjahan Mehzabeen, Harini Shandilya, Keith Rickert, Rebecca Croasdale-Wood, Melissa Damschroder, Ying Fu, Andrew Dippel, Jeffrey J. Gray, Gilad Kaplan

“…High-quality structural information remains critical for the antibody optimization process, but antibody-antigen complex structures are often unavailable and in silico antibody docking methods are still unreliable. …”
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PfCSP-ferritin nanoparticle malaria vaccine antigen formulated with aluminum-salt and CpG 1018® adjuvants: Preformulation characterization, antigen-adjuvant interactions, and mouse immunogenicity studies by John M. Hickey, Nitya Sharma, Max Fairlamb, Jennifer Doering, Yetunde Adewunmi, Katherine Prieto, Giulia Costa, Benjamin Wizel, Elena A. Levashina, Nicholas J. Mantis, Jean-Philippe Julien, Sangeeta B. Joshi, David B. Volkin

“…A competitive ELISA was developed to assess antigen binding to a PfCSP-specific mAb. The in vitro antigenicity of the 155 antigen was measured upon formulation with adjuvants, including aluminum-salts (i.e. …”
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Chimeric antigen receptor with novel intracellular modules improves antitumor performance of T cells by Pengju Wang, Yiyi Wang, Xiaojuan Zhao, Rui Zheng, Yiting Zhang, Ruotong Meng, Hao Dong, Sixin Liang, Xinyi He, Yang Song, Haichuan Su, Bo Yan, An-Gang Yang, Lintao Jia

“…Abstract The excessive cytokine release and limited persistence represent major challenges for chimeric antigen receptor T (CAR-T) cell therapy in diverse tumors. …”
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Neuroinvasive Cryptococcosis in an Immunocompetent Patient with a Negative Spinal Fluid Cryptococcus Antigen by Rocio C. Garcia-Santibanez, Veenu Gill, Stanley Yancovitz, DeWitt Pyburn

“…Brain MRI showed meningeal enhancement and herniation. Serum Cryptococcus antigen was positive but spinal fluid antigen and cultures were negative. …”
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Flow Cytometric Analysis of T, B, and NK Cells Antigens in Patients with Mycosis Fungoides by Serkan Yazıcı, Emel Bülbül Başkan, Ferah Budak, Barbaros Oral, Şaduman Balaban Adim, Zübeyde Ceylan Kalin, Güven Özkaya, Kenan Aydoğan, Hayriye Saricaoğlu, Şükran Tunali

“…We retrospectively analyzed the clinicopathological correlation and prognostic value of cell surface antigens expressed by peripheral blood mononuclear cells in patients with mycosis fungoides (MF). 121 consecutive MF patients were included in this study. …”
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Surveillance Following Hepatitis B Surface Antigen Loss: An Issue Requiring Attention by Shuai-Wen Huang, Hong Long, Jia-Quan Huang

“…The latest guidelines recommend (hepatitis B surface antigen) HBsAg loss as the ideal treatment target for improving liver function, histopathology, and long-term prognosis. …”
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Intrauterine Growth Restriction: Cytokine Profiles of Trophoblast Antigen-Stimulated Maternal Lymphocytes by Raj Raghupathy, Majedah Al-Azemi, Fawaz Azizieh

“…We studied cytokine production by maternal peripheral blood lymphocytes stimulated by trophoblast antigens. 36 women with a diagnosis of IUGR and 22 healthy women with normal fetal growth were inducted. …”
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Naturally Acquired Antibody Responses to a Synthetic Malaria Antigen AS202.11 by Rebeka Nazareth, Pius Horumpende, Tolbert Sonda, Arnold Ndaro, Edson Mollel, Eliakim Paul, Emmanuel Athanase, Jaffu Chilongola

“…We report a high degree of recognition of AS202.11 by IgG elicited by field P. falciparum strains, suggesting its close similarity to native P. falciparum antigens and possible suitability of the peptide as a future malaria vaccine candidate.…”
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Interrelationship of the Kinin System, Nitric Oxide and Eicosanoids in the Antigen-Induced Arthritis in Rabbits by Fernando Alfonso Salamea Palácios, Gilberto Santos Novaes, Maria Luiza Guzzo, Ieda Maria Magalhães Laurindo, Suzana Beatriz Verissimo de Mello

“…The aim of the present study was to investigate the interrelationship of the kinin system, nitric oxide and eicosanoids in the acute phase of antigen-induced arthritis (AIA) in rabbits. The arthritis was induced in immunized rabbits and the following parameters were evaluated 24 hours later: leukocyte influx (total and differential white cell count), vascular permeability (Evans's blue method), and synovial PMN cell infiltrate. …”
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Preparation of recombinant myoglobin and investigation of the liquid antigen stability for quality control materials by Yu-Hui Wang, Xi-Feng Sun, Chun-Xin Xu, Feng-Qiang Sun, Rong-Rong Wang, Xiao-Kun Bian, Zhan-Zhao Wang, Qiang Wu

“…The concentrations per gradient measured using different kits had no significant difference (p-value>0.05), which indicated that the reactivity between the Mb antigen and Mb antibodies with different epitopes was good. …”
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Blastomyces dermatitidis Yeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis by Alex R. Boyd, Jamie L. VanDyke, Gene M. Scalarone

“…All of the 6 antigenic preparations were able to detect antibody in the serum specimens, but the antigen combinations detected antibody to a higher degree than the individual antigen. …”
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Mapping naturally presented T cell antigens in medulloblastoma based on integrative multi-omics by Julia Velz, Lena K. Freudenmann, Gioele Medici, Marissa Dubbelaar, Malte Mohme, David R. Ghasemi, Jonas Scheid, Daniel J. Kowalewski, Angelica B. Patterson, Anna M. Zeitlberger, Katrin Lamszus, Manfred Westphal, Matthias Eyrich, Martina Messing-Jünger, Andreas Röhrig, Harald Reinhard, Kévin Beccaria, Rogeiro B. Craveiro, Beat M. Frey, Martin Sill, Sven Nahnsen, Marie Gauder, Konstantina Kapolou, Manuela Silginer, Tobias Weiss, Hans-Georg Wirsching, Patrick Roth, Michael Grotzer, Niklaus Krayenbühl, Oliver Bozinov, Luca Regli, Hans-Georg Rammensee, Elisabeth J. Rushing, Felix Sahm, Juliane S. Walz, Michael Weller, Marian C. Neidert

“…Here, we present an atlas of naturally presented T cell antigens in medulloblastoma. We map the human leukocyte antigen (HLA)-presented peptidomes of 28 tumors and perform comparative immunopeptidome profiling against an in-house benign database. …”
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Effects of different vaccination regimes on the immunodiagnosis of tuberculosis in goats and evaluation of defined antigens by Patricia Cuenca-Lara, Patricia Cuenca-Lara, Miriam Blay-Benach, Miriam Blay-Benach, Zoraida Cervera, Zoraida Cervera, Cristian Melgarejo, Cristian Melgarejo, Julia Moraleda, Julia Moraleda, Iker A. Sevilla, Joseba M. Garrido, Mahavir Singh, Gareth J. Jones, Bernat Pérez de Val, Bernat Pérez de Val

“…In addition, the defined antigens ESAT-6, CPF10, and EspC were evaluated as alternative diagnostic reagents to PPDs. …”
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Immune Responses to Tissue-Restricted Nonmajor Histocompatibility Complex Antigens in Allograft Rejection by Ankit Bharat, T. Mohanakumar

“…Chronic diseases that result in end-stage organ damage cause inflammation, which can reveal sequestered self-antigens (SAgs) in that organ and trigger autoimmunity. …”
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Identifying plasma exosome antigens as a potential diagnostic biomarker for tuberculosis disease by Yan Jiang, Li Yan, Baoru Zhou, Jianming Hong, Huixing Tan, Xiaonan Li, Yong Wang

“…The concentration and diameter of exosomes from different groups were detected using NTA. The antigen Ag85A, MPT64, CFP-10 of Mtb in exosomes was detected by ELISA. …”
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