Application of quantitative protein mass spectrometric data in the early predictive analysis of membrane-bound target engagement by monoclonal antibodies by Armin Sepp, Morris Muliaditan

“…In the case of monoclonal antibodies (mAbs) against cell membrane antigens, this requires quantitative insight into the target tissue concentration levels. …”
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The Immunological Plasmodium falciparum Malaria Characteristics of Children in Tajikistan Republic by Nighina M. Khodzhaeva, Alla M. Baranova, Anatoly K. Tokmalaev

“…The imbalance of cytokine profile is an important pathogenic factor in the development of severe and recurrent forms of the disease, since the formation of a defective immune response to parasitic antigens contributes to adverse outcomes. Conclusions. …”
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Effect of innate and induced immunity on infectious bursal disease pathogenesis by A. N. Semina

“…Being the RNA-virus, the agent is specified by high frequency of mutations, which result in the emergence of the strains with modified antigenicity and increased virulence. The molecular basis for the virus pathogenicity and exact cause of the clinical disease and death are still understudied as they are not clearly associated with the disease severity and degree of bursa of Fabricius lesions. …”
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Hep-2000 indirect immunofluorescence assay – An emerging screening tool for detection of antinuclear autoantibodies in various connective tissue disorders by Arpita Singh, Shilpi Sahu, Neha R. Jadhav, Navdeep Kaur

“…Antinuclear Antibodies (ANA) directed against a variety of nuclear antigens detectable in the serum are used for screening, diagnoses, and monitoring of autoimmune diseases, with immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA) being the most widely used methods. …”
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Decreased Circulating T Regulatory Cells in Egyptian Patients with Nonsegmental Vitiligo: Correlation with Disease Activity by Doaa Salah Hegab, Mohamed Attia Saad Attia

“…Dysregulation of Tregs may be one of the factors that can break tolerance to melanocyte self-antigens and contribute to vitiligo pathogenesis. Methods. …”
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diaTracer enables spectrum-centric analysis of diaPASEF proteomics data by Kai Li, Guo Ci Teo, Kevin L. Yang, Fengchao Yu, Alexey I. Nesvizhskii

“…We demonstrate the performance of diaTracer and FragPipe using diaPASEF data from triple-negative breast cancer, cerebrospinal fluid, and plasma samples, data from phosphoproteomics and human leukocyte antigens immunopeptidomics experiments, and low-input data from a spatial proteomics study. …”
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Association of Toll-Like Receptor 2 (TLR2) Expression in Placenta and Intrauterine Exposure to Hepatitis B Virus by Dian Utami, Maisuri T. Chalid, Rina Masadah, Rizalinda Sjahril, Andi Dwi Bahagia Febriani

“…It has been known that toll-like receptors (TLRs) on the placenta respond to antigens, such as zymosan, lipopolysaccharide, and other viral infections. …”
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HLA Class II Defects in Burkitt Lymphoma: Bryostatin-1-Induced 17 kDa Protein Restores CD4+ T-Cell Recognition by Azim Hossain, Jason M. God, Faisal F. Y. Radwan, Shereen Amria, Dan Zhao, Jennifer R. Bethard, Azizul Haque

“…We further demonstrate that BL cells express the components of the class II pathway, and the defect was not caused by faulty Ag/class II interaction, because antigenic peptides bound with measurable affinity to BL-associated class II molecules. …”
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Application of diagnostic methods in the verification of Lyme carditis (clinical case) by K.V. Myndziv, N.I. Yarema, N.Ya. Vereshchahina, V.I. Bondarchuk, U.P. Hevko, O.V. Vayda

“…Total antibodies to antigens of the B. burgdorferi sensu lato complex in blood serum were determined by the ELISA method with test systems Euroimmun AG (Germany). …”
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Long-Term Cultured Human Term Placenta-Derived Mesenchymal Stem Cells of Maternal Origin Displays Plasticity by Vikram Sabapathy, Saranya Ravi, Vivi Srivastava, Alok Srivastava, Sanjay Kumar

“…These cells did not significantly alter cell cycle or apoptosis pattern while maintaining the normal karyotype; they also have limited expression of MHC-II antigens and are Naive for stimulatory factors CD80 and CD 86. …”
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12 Weeks of Combined Endurance and Resistance Training Reduces Innate Markers of Inflammation in a Randomized Controlled Clinical Trial in Patients with Multiple Sclerosis by Nathalie Deckx, Inez Wens, Amber H. Nuyts, Niel Hens, Benedicte Y. De Winter, Gudrun Koppen, Herman Goossens, Pierre Van Damme, Zwi N. Berneman, Bert O. Eijnde, Nathalie Cools

“…Interestingly, the number of CD80+ pDC positively correlates with the presence of IL-10-producing regulatory type 1 cells (Tr1), an important cell type for maintaining peripheral tolerance to self-antigens. In addition, decreased production of the inflammatory mediators, TNF-α and MMP-9, upon Toll-like receptor (TLR) stimulation was found at the end of the exercise program. …”
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PEGDA-based HistoBrick for increasing throughput of cryosectioning and immunohistochemistry in organoid and small tissue studies by Emilie Vuille-dit-Bille, Larissa Utz, Fiona E. Müllner, Valeria J. Arteaga-Moreta, Yanyan Hou, Stefan E. Spirig, Diane Ledroit-Paic, Sarah Heub, Jonas Goldowsky, Gilles Weder, Magdalena Renner

“…Immunostaining on thin tissue sections enables precise visualization of antigens and proteins. However, for cryosectioning small tissues such as organoids, spheroids, and tumoroids there is a lack of standardized, time- and cost-effective methods, limiting the throughput of analysis. …”
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Gastric Carcinoma with Osteoclast-Like Giant Cells Coexisting with Gastrointestinal Spindle Cell Tumor by Christos Poulios, Triantafyllia Koletsa, Antonis Goulas, Georgia Karayannopoulou, Eleni Vrettou, Ioannis Kostopoulos

“…Moreover, in a random section from the gastric fundus, a spindle cell lesion, sized 0.6 cm, was revealed, which was positive for CD117 and CD34 antigens and was diagnosed as gastrointestinal stromal tumor (GIST). …”
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Development of a Novel Sensor System Based on Magnetic Microspheres to Detect Cardiac Troponin T by Junrong Zhang, Hongxiang Liu, Baofeng Xu, Sijun Huang, Rui Liu, Jinming Zhu, Yi Guo, Li Xu

“…Naive human cTnT protein in serum samples and antigens on functional magnetic microspheres will competitively bind with limited specific antibodies. …”
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The amino acids differences in epitopes may promote the different allergenicity of ovomucoid derived from hen eggs and quail eggs by Mengzhen Hao, Shuai Yang, Shiwen Han, Huilian Che

“…The difference in sensitization compare to hen egg ovomucoid may be due to amino acids differences affecting protein structure by changing antigenic epitopes.…”
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Engineered a dual-targeting biomimetic nanomedicine for pancreatic cancer chemoimmunotherapy by Meng Wang, Qida Hu, Junmin Huang, Xinyu Zhao, Shiyi Shao, Fu Zhang, Zhuo Yao, Yuan Ping, Tingbo Liang

“…To this end, we developed a biomimetic dual-targeting nanomedicine (PG@KMCM) where gemcitabine-loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles are coated with a layer of bioengineered cancer cell membrane that stably expresses peptides targeting M2-like macrophages (M2pep) while reserving tumor-associated antigens (TAAs). The PG@KMCM nanomedicine enables the simultaneous targeted delivery of gemcitabine to pancreatic tumor sites and TAMs to potentiate its therapeutic effects. …”
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Attempted Depletion of Passenger Leukocytes by Irradiation in Pigs by Hao-Chih Tai, Xiaocheng Zhu, Yih Jyh Lin, Hidetaka Hara, Mohamed Ezzelarab, Michael Epperley, Mubina A. Quader, David K. C. Cooper

“…The lack of effect may have been related to (i) inadequate depletion of passenger leukocytes, thus not preventing a direct T cell response, (ii) the presence of dead or dying leukocytes (antigens), thus not preventing an indirect T cell response, or (iii) constitutive expression of MHC class II and B7 molecules on the porcine vascular endothelium, activating recipient T cells.…”
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Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases by Athanasios Mavropoulos, Dimitrios P. Bogdanos, Christos Liaskos, Timoklia Orfanidou, Theodora Simopoulou, Efterpi Zafiriou, Lazaros I. Sakkas, Eirini I. Rigopoulou

“…To overcome these limitations, we have modified methanol-based phosphoflow protocols using several commercially available antibody clones suitable for surface antigens, intracellular cytokines, and p38 MAPK. These included markers of B cells (CD19, CD20, and CD22), T cells (CD3, CD4, and CD8), NK (CD56 and CD7), and dendritic cells (CD11c). …”
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A Model of Insulin Resistance in Mice, Born to Diabetic Pregnancy, Is Associated with Alterations of Transcription-Related Genes in Pancreas and Epididymal Adipose Tissue by Akadiri Yessoufou, Kabirou Moutairou, Naim Akhtar Khan

“…Though IR offspring exhibited reduced epididymal adipose tissue, their adipocytes seemed to be differentiated into macrophage-like cells, as they exhibited upregulated CD14 and CD68 antigens, generally expressed by macrophages. However, there was no peripheral macrophages infiltration into epididymal adipose tissue, as the expression of F4/80, a true macrophage marker, was undetectable. …”
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The Increase of Circulating PD-1- and PD-L1-Expressing Lymphocytes in Endometriosis: Correlation with Clinical and Laboratory Parameters by Monika Walankiewicz, Ewelina Grywalska, Grzegorz Polak, Izabela Korona-Glowniak, Elzbieta Witt, Agata Surdacka, Jan Kotarski, Jacek Rolinski

“…Expression of PD-1 and PD-L1 on T and B cells could represent the hallmark of immune system reaction to chronic antigenic exposition in patients with endometriosis.…”
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