Standardised protocol for a prospective cross-sectional multicentre clinical utility evaluation of two dual point-of-care tests in non-clinical settings for the screening of HIV an... by James Kiarie, Karel Blondeel, Igor Toskin, Jordi Casabona, Soe Soe Thwin, Massimo Mirandola, Rosanna Peeling, Ronald Ballard, Laura Fernàndez-López, Inga Upmace, Juliana Reyes-Urueña, Mara Vaselova, Mitja Ćosić, Simon Maljevac, William Mejías, Ander Pazos, Andrii Chernyshev

“…Introduction Point-of-care dual tests (POCTs) for simultaneously detecting antibodies to HIV and syphilis (dual HIV-syphilis POCTs) have been developed recently and show encouraging performance compared with the reference tests in laboratory-based studies. …”
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Comparative evaluation of funduscopy, PCR and serology in the diagnosis of ocular toxoplasmosis at ECWA Eye Hospital, Kano by Biodun Bintu Adebakin, Mayor Orezime Atima, Ugbede Idakwo, Jamilu Abubakar Bala, Alhasan Sharif Abdullahi, Auwal Bello Muhammad, Jah Douglas Pam, Emeka John Dingwoke, Florence Olufunke Darda

“…Serological analysis was conducted using ELISA to detect anti-Toxoplasma IgG antibodies. Additionally, PCR was used to confirm the presence of T. gondii DNA in blood samples. …”
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Evaluation of a PCR-based lateral flow device for detecting Aspergillus and Candida species from clinical specimens by Ariff Khalid, Jacinta Santhanam, Mohd Nizam Tzar, Ang-Lim Chua, Sharifah Fadilah Abdul Wahid, Wan Rahiza Wan Mat, Raha Abd Rahman, Petrick Periyasamy

“…The LFA dipstick, in a modified sandwich format, utilises immobilised antibodies complementary to the fluorophore labels on the PCR products, and gold nanoparticles to form a visible red line that indicates the presence of the targeted fungus. …”
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Anti‐retinal Autoantibodies in Hydroxychloroquine Eye Toxicity by Samuel D. Good, Grazyna Adamus, Michael B. Gorin, Jordan Jacquez, Jennifer Grossman, Isao Matsuura, Ashira Hasan, Brian Skaggs, Maureen McMahon

“…In multivariate analysis accounting for risk factors associated for HCQ eye toxicity, the presence of anti‐arrestin antibodies was associated with a 3.2‐fold increase in the odds of developing HCQ eye toxicity. …”
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Alterations in the social-conditioned place preference and density of dopaminergic neurons in the ventral tegmental area in Clsnt2-KO mice by I. N. Rozhkova, S. V. Okotrub, E. Yu. Brusentsev, K. E. Uldanova, E. A. Chuyko, V. A. Naprimerov, T. V. Lipina, T. G. Amstislavskaya, S. Ya. Amstislavsky

“…At the same time, an increased number of DA and possibly GABA neurons labeled with antibodies against the type 2 dopamine receptor as well as against tyrosine hydroxylase were detected in the VTA of the Clstn2-KO mice. …”
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GFAPβ and GFAPδ Isoforms Expression in Mesenchymal Stem Cells, MSCs Differentiated Towards Schwann-like, and Olfactory Ensheathing Cells by Nidia Jannette Carrillo González, Gabriela Stefania Reyes Gutierrez, Tania Campos-Ordoñez, Rubén D. Castro-Torres, Carlos Beas Zárate, Graciela Gudiño-Cabrera

“…First, the characterization phenotyping was verified by morphology and immunocytochemistry, using p75, CD90, and GFAP antibodies. Then, we found the expression of GFAP isoforms (α, β, and δ) in the three conditions; the expression of the GFAPα (10.95%AUC) and GFAPβ (9.17%AUC) isoforms was predominantly in OECs, followed by dBM-MSCs (α: 3.99%AUC, β: 5.66%AUC) and uBM-MSCs (α: 2.47%AUC, β: 2.97%AUC). …”
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HUBUNGAN EKSPRESI NEGATIF MLH-1 DENGAN GAMBARAN KLINIKOPATOLOGIK KARSINOMA KOLOREKTAL by Selly Alinta Syukri, Tofrizal Tofrizal, Aswiyanti Asri, Salmiah Agus

“…Sampel diambil dari blok paraffin jaringan pembedahan, diklasifikasikan berdasarkanusia, jenis kelamin, lokasi tumor dan subtipe histopatologik. Antibodi MLH-1diproduksioleh D Bio System (DBS). Pewarnaan dilakukan untuk menentukan ada/ tidaknya ekpresi MLH-1 pada inti sel tumor. …”
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Evaluation of Immune Characteristics and Factors Associated with Immune Response following Hepatitis B Vaccination among Ghanaian Adolescents by Samuel Asamoah Sakyi, Joseph Badu Gyapong, Ebenezer Krampah Aidoo, Alfred Effah, Simon Koffie, Oscar Simon Olympio Mensah, Isaac Arddey, Godwin Boakye, Stephen Opoku, Benjamin Amoani, Robert Amadu Ngala

“…Approximately two thirds of adolescents vaccinated at birth lack protective levels of antibodies against hepatitis B virus. Booster vaccines could aid in mounting protective levels of anti-HBsAg. …”
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Intestinal obstruction caused by disseminated mycobacterium avium complex disease following solid organ transplantation: a case report by Akane Mita, Sho Nakakubo, Yusuke Nishimura, Hideki Shima, Masaaki Watanabe, Tsuyoshi Shimamura, Satoshi Konno

“…Disseminated MAC disease occurs mainly in immunocompromised individuals, such as those with acquired immunodeficiency syndrome, hematological malignancies, or those positive for anti-interferon-γ antibodies. However, its occurrence in solid organ transplant recipients is uncommon. …”
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High Seroprevalence of Autoantibodies Typical of Autoimmune Liver Disease in Eastern Ethiopia: Is Chewing of Khat (Catha edulis) a Triggering Factor? by Stian Magnus Staurung Orlien, Tekabe Abdosh Ahmed, Nejib Yusuf Ismael, Nega Berhe, Trine Lauritzen, Svein Gunnar Gundersen, Asgeir Johannessen

“…Seroprevalence of antinuclear (ANA), antismooth muscle (SMA), and antimitochondrial antibodies (AMA) were determined and compared between the groups using logistic regression models to adjust for age and sex. …”
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High prevalence of undocumented SARS-CoV-2 infections revealed by analysis of nucleocapsid-specific IgG responses in diagnosed and undiagnosed individuals. by Kim Blom, Ilias Galanis, Philip Bacchus, Klara Sondén, Ioana Bujila, Tatiana Efimova, Fredrik Garli, Mikael Mansjö, Elin Movert, Aleksandra Pettke, Marie Rapp, Maike Sperk, Sandra Söderholm, Karin Valentin Asin, Sarah Zanetti, Magnus Gisslén, Andreas Bråve, Ramona Groenheit, Jonas Klingström

“…SARS-CoV-2 can induce spike and nucleocapsid protein specific IgG antibodies, which can be detected in seroprevalence studies to identify a previous infection. …”
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Mutations in the <i>A34R</i> gene increase the immunogenicity of vaccinia virus by S. N. Shchelkunov, T. V. Bauer, S. N. Yakubitskiy, A. A. Sergeev, A. S. Kabanov, S. A. Pyankov

“…It was shown that increase in EEV production by recombinant strain VACV LIVP-GFP-A34R does not change the attenuated phenotype characteristic of the parental strain LIVP-GFP, but causes a significantly larger production of VACV-specific antibodies.…”
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Egfr Amplification Specific Gene Expression in Phyllodes Tumours of the Breast by Konstantin Agelopoulos, Christian Kersting, Eberhard Korsching, Hartmut Schmidt, Arno Kuijper, Christian August, Pia Wülfing, Joke Tio, Werner Boecker, Paul J. van Diest, Burkhard Brandt, Horst Buerger

“…The results were verified and extended by means of immunohistochemistry using the tissue microarray method on an extensively characterized series of 58 phyllodes tumours with antibodies against caveolin-1, eps15, EGF, TGF-α, pErk, pAkt and mdm2. …”
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Efficacy and Safety of Ergoferon in Children from 6 Months to 6 Years Old with Acute Respiratory Viral Infections in Contemporary Outpatient Practice: A Multicenter, Double-Blind,... by N. A. Geppe, B. M. Blokhin, O. V. Shamsheva, S. T. Abdrakhmanova, K. A. Alikhanova, G. T. Myrzabekova

“…Derived by technological treatment of antibodies to interferon gamma, histamine, and CD4, Ergoferon was previously proved to modulate its molecular targets promoting effective antiviral protection. …”
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Detection of Phosphorylated Alpha-Synuclein in the Muscularis Propria of the Gastrointestinal Tract Is a Sensitive Predictor for Parkinson’s Disease by Goichi Beck, Yumiko Hori, Yoshito Hayashi, Eiichi Morii, Tetsuo Takehara, Hideki Mochizuki

“…Immunohistochemistry was performed using primary antibodies against α-synuclein (αSyn) and phosphorylated αSyn (pαSyn), after which Lewy pathology in each sample was assessed. …”
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Prognostic importance of human leukocyte antigen DRβ1 gene and protein tyrosine phosphatase nonreceptor Type 22 gene polymorphism in rheumatoid arthritis by Pramod Kumar Verma, Usha Singh, Shyam Kumar Saraf, Narendra Kumar Singh

“…HLA DRβ1 typing was done by low-resolution sequence-specific primer polymerase chain reaction and auto-antibodies (Abs) (rheumatoid factor [RF], anti-cyclic citrullinated peptide 2 (CCP2) Ab, anti-nuclear Ab, double-stranded deoxyribose nucleic acid, anticardiolipin Ab) were done by enzyme-linked immunosorbent assay. …”
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Thyroid Hormone Changes in the Northern Area of Tianjin during the COVID-19 Pandemic by Dong Weiwei, Wu Bei, Wang Hong, Wu Cailan, Shao Hailin, Xu Donghong, Wang Xiaolai, Hao Zhaohu, Li Shijun, Tan Jian, Jia Qiang

“…The detection rate of abnormal thyroid function was decreased in pandemic Group B (69.2% vs. 93.3%, χ2 = 42.725, p<0.01), especially for hypothyroidism (14.29% vs. 35.71%, χ2 = 27.429, p<0.01) and isolated thyroid-related antibodies (25.89% vs. 38.39%, χ2 = 8.023, p<0.01). …”
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Chitinase-3-like-1: a multifaceted player in neuroinflammation and degenerative pathologies with therapeutic implications by Pharaoh Fellow Mwale, Cheng-Ta Hsieh, Ting-Lin Yen, Jing-Shiun Jan, Rajeev Taliyan, Chih-Hao Yang, Wen-Bin Yang

“…Furthermore, therapeutic strategies targeting CHI3L1, such as small-molecule inhibitors and neutralizing antibodies, have shown promise in preclinical studies, demonstrating reduced neuroinflammation, amyloid plaque accumulation, and improved neuronal survival. …”
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Identification of a pathogenic founder variant in the WFS1 gene that causes Wolfram syndrome in the Druze population by Inbal Halabi, Inbal Halabi, Yardena Tenenbaum-Rakover, Yardena Tenenbaum-Rakover, Lena Sagi-Dain, Lena Sagi-Dain, Lena Sagi-Dain, Ilana Koren, Ilana Koren, Ilana Koren

“…Our findings imply that molecular analysis is warranted in children presenting with DM and negative pancreatic antibodies. The identified variant should be considered for genetic testing in individuals of Druze ancestry diagnosed with young-onset non-autoimmune diabetes. …”
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Patient-reported systemic symptoms in women with silicone breast implants: a descriptive cohort study by Karlinde Amber Spit, Miranda Scharff, Christel JM de Blok, Frank B Niessen, Yara Bachour, Prabath W Nanayakkara

“…Furthermore, 56% reported pre-existing allergies at baseline and positive antinuclear antibodies were observed in 23%. At follow-up with a median of 3.3 years (IQR 2–4), 152 women had their implants removed on clinical grounds. …”
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